LOCUS       sequence-320094-       10104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This insert, when expressed in the same cell with other split Cre 
            component, forms Cre recombinase through protein trans-splicing 
            using split intein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Luan H, Kuzin A, Odenwald WF, White BH
  TITLE     Cre-assisted fine-mapping of neural circuits using orthogonal split 
            inteins.
  JOURNAL   Elife. 2020 Apr 14;9. pii: 53041. doi: 10.7554/eLife.53041.
  PUBMED    32286225
REFERENCE   2  (bases 1 to 10104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-04-14"; volume: "9. pii: 53041. doi"; pages: " 
            10.7554/eLife.53041"
COMMENT     Sequence Label: HJP-177-IVS-Syn21-gp41-1C-CreBC-WPREw
FEATURES             Location/Qualifiers
     source          1..10104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     267..285
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    510..579
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(666..1254)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(746..765)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1425..2216,2217..2285))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2048..2067
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2286..2390)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2457..2475
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    2503..2627
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2652..2657,2658..2675,2676..2682)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2736..3395
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2802..2821)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3737..4042
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3921..3940
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4083..4207)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          4246..4312
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     misc_feature    5171..5759
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5224..5244)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5642..5653)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6020..6036)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6020..6036)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(7047..7661,7732..7863,8067..8382,8444..9098,
                     9173..9446,9848..9919))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 cattgacgct acgtaacgct acaaacggtg gcgaaagaga tagcggacgc agcggcgaaa
       61 gagacggcga tatttctgtg gacagagaag gaggcaaaca gcgctgactt tgagtggaat
      121 gtcattttga gtgagaggta atcgaaagaa cctggtacat caaataccct tggatcgaag
      181 taaatttaaa actgatcaga taagttcaat gatatccagt gcagtaaaaa aaaaaaatgt
      241 tttttttatc tactttccgc aaaaatgggt tttattaact tacatacata ctaggcgcgc
      301 ccatatgttc ggcttgtcga catgcccgcc gtgaccgtcg agaacccgct gacgctgccc
      361 cgcgtatccg cacccgccga cgccgtcgca cgtcccgtgc tcaccgtgac caccgcgccc
      421 agcggtttcg agggcgaggg cttcccggtg cgccgcgcgt tcgccgggat caactaccgc
      481 cacctcgacc cgttcatcat gatggaccag atgggtgagg tggagtacgc gcccggggag
      541 cccaagggca cgccctggca cccgcaccgc ggcttcgaga ccgtgaccta catcgtcgac
      601 ggtaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      661 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      721 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      781 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      841 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      901 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      961 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1021 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1081 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1141 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1201 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1261 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1321 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1381 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1441 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1501 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1561 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1621 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1681 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1741 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1801 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1861 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     1921 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     1981 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2041 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     2101 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2161 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     2221 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     2281 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2341 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2401 cgaaaagtgc cacctgagtc taagaaacca ttattatcat gacattaacc tataaaaata
     2461 ggcgtatcac gaggcccttt cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa
     2521 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
     2581 aaacagacta cataatactg taaaacacaa catatccagt cactatggcg gccgcattag
     2641 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg
     2701 atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
     2761 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     2821 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     2881 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     2941 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     3001 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     3061 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     3121 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     3181 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     3241 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     3301 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     3361 aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc ttactaaaag
     3421 ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata
     3481 tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt
     3541 tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta
     3601 agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat
     3661 caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag
     3721 aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg
     3781 tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc
     3841 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
     3901 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt
     3961 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
     4021 cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg
     4081 accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     4141 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     4201 aagtggtgat aaacggccgg ccagatctgg taccgctaga aaaaggtagg ttcaaccact
     4261 gatgcctagg cacaccgaaa cgactaaccc taattcttat cctttacttc agggctagca
     4321 acttaaaaaa aaaaatcaaa atgatgctga agaagatcct gaagatcgag gagctggatg
     4381 agcgcgagct gatcgatatc gaggtgagcg gcaaccacct gttctacgcc aacgatatcc
     4441 tgacccacaa cagcaatgca gtgagcctgg taatgcgtcg aatacgcaag gaaaacgtgg
     4501 acgccggcga gagggccaaa caggcgttgg ctttcgaacg caccgatttc gatcaggtgc
     4561 ggtcgttgat ggagaacagc gatcgatgcc aggacatccg gaacttggcg tttttgggca
     4621 ttgcctataa caccctcctt cgcatcgccg agatagcgcg tatccgagtt aaagacattt
     4681 cccgtacaga cggaggccgc atgcttattc acatcggacg cacgaaaacc ctggtcagca
     4741 ctgctggcgt ggagaaagca ctttccctgg gtgtgacgaa attggtcgag cgatggatca
     4801 gtgtgagtgg cgtcgccgat gaccccaaca attatctgtt ctgcagggtg cgcaaaaatg
     4861 gagtggcagc cccctcggca acaagtcagt tgagtacgcg cgcgttggag ggaatcttcg
     4921 aggccactca ccgattgatt tacggtgcaa aagatgactc cggccaacgt tacttggcct
     4981 ggtccggtca cagcgcacgt gtgggtgccg cccgtgacat ggcgagggcc ggtgtttcca
     5041 tcccagaaat catgcaagcg ggcggatgga ccaacgtgaa tattgtaatg aactacattc
     5101 gtaatctgga tagtgaaaca ggcgcaatgg tgcgcctgct ggaggacgga gattaaaccg
     5161 gtgtttaaac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5221 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5281 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5341 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5401 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5461 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     5521 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     5581 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     5641 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     5701 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     5761 tgaagcttat cgataccgtc gactaaagcc aaatagaaaa ttattcagtt cctggcttaa
     5821 gtttttaaaa gtgatattat ttatttggtt gtaaccaacc aaaagaatgt aaataactaa
     5881 tacataatta tgttagtttt aagttagcaa caaattgatt ttagctatat tagctacttg
     5941 gttaataaat agaatatatt tatttaaaga taattgcgtt tttattgtca gggagtgagt
     6001 ttgcttaaaa actcgtttag atccactagt tctagagcgg ccgctggcca cgggtgcgca
     6061 tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc
     6121 agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga
     6181 cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga
     6241 agtcagcgcc ctgcaccatt atgttccgga ggcgcgccct agttccagtg aaatccaagc
     6301 attttctaaa ttaaatgtat tcttattatt atagttgtta tttttgatat atataaacaa
     6361 cactattatg cccaccattt ttttgagatg catctacaca aggaacaaac actggatgtc
     6421 actttcagtt caaattgtaa cgctaatcac tccgaacagg tcacaaaaaa ttaccttaaa
     6481 aagtcataat attaaattag aataaatata gctgtgaggg aaatatatac aaatatattg
     6541 gagcaaataa attgtacata caaatattta ttactaattt ctattgagac gaaatgaacc
     6601 actcggaacc atttgagcga accgaatcgc gcggaactaa cgacagtcgc tccaaggtcg
     6661 tcgaacaaaa ggtgaatgtg ttgcggagag cgggtgggag acagcgaaag agcaactacg
     6721 aaacgtggtg tggtggaggt gaattatgaa gagggcgcgc gatttgaaaa gtatgtatat
     6781 aaaaaatata tcccggtgtt ttatgtagcg ataaacgagt ttttgatgta aggtatgcag
     6841 gtgtgtaagt cttttggtta gaagacaaat ccaaagtcta cttgtgggga tgttcgaagg
     6901 ggaaatactt gtattctata ggtcatatct tgtttttatt ggcacaaata taattacatt
     6961 agctttttga gggggcaata aacagtaaac acgatggtaa taatggtaaa aaaaaaaaac
     7021 aagcagttat ttcggatata tgtcggctac tccttgcgtc gggcccgaag tcttagagcc
     7081 agatatgcga gcacccggaa gctcacgatg agaatggcca gacccacgta gtccagcggc
     7141 agatcggcgg cggagaagtt aagcgtctcc aggatgacct tgcccgaact ggggcacgtg
     7201 gtgttcgacg atgtgcagct aatttcgccc ggctccacgt ccgcccattg gttaatcagc
     7261 agaccctcgt tggcgtaacg gaaccatgag aggtacgaca accatttgag gtatactggc
     7321 accgagcccg agttcaagaa gaagccgcca aagagcagga atggtatgat aaccggcgga
     7381 cccacagaca gcgccatcga ggtcgaggag ctggcgcagg atattagata tccgaaggac
     7441 gttgacacat tggccaccag agtgaccagc gccaggcagt tgaagaagtg cagcactccg
     7501 gcccgcagtc cgatcatcgg ataggcaatc gccgtgaaga ccagtggcac tgtgagaaaa
     7561 agcggcaatt cggcaatcgt tttgcccaga aagtatgtgt cacagcgata aagtcgactt
     7621 cgggcctccc tcataaaaac tggcagctct gaggtgaaca cctaaatcga atcgattcat
     7681 tagaaagtta gtaaattatt gaaatgcaaa tgtattctaa acatgactta catttatcgt
     7741 ggcaaagacg ttttgaaagg tcatgttggt caggaagagg aagatggctc cgttgatatt
     7801 catcacaccc acttgcgtga gttgttggcc caaaaagatg aggccaatca agatggcaac
     7861 catctgcaaa ttaaaatgtt actcgcatct cattaatatt cgcgagttaa atgaaattta
     7921 tttatcttct gcaaaactat aaactataca tctcattgaa aaaaactaag aagggtgtgg
     7981 aatcaggcaa ttctatctaa aatctagcga atttgtttcc aagaattgta agcgttatat
     8041 catttgtttc cactggaacc actcaccgtt gtctgaataa gtcgcacttt tacgaggagt
     8101 ggttccttga gcaccgacag ccaggatcgc cacaggaccg cccggaactg catgaaccag
     8161 gtggccttgt aggtgtaccc attctccggc tgctccagtg gcttctccag atttttggtg
     8221 gccaacaact gctccatatc ccgggctact ttgctaatgg caaaattgtc gcatatcttg
     8281 gcgatccgat cacgggactc gatctcccgt ccgggcacaa cggccaacac ctgtacgtaa
     8341 aagtccgccg gattgtagtt ggtaggacac tgggcaccca cgctggatag gagttgagat
     8401 gttatgtaat actagatacc cttaataaac acatcgaact cactaggaaa agaagtcgac
     8461 ggcttcgctg ggagtgccca agaaagctac cctgccctcg gccatcagaa ggatcttgtc
     8521 aaagagctca aacagctcgg aagacggctg atgaatggtc aggatgacgg tcttgccctt
     8581 ctgcgacagc ttcttcagca cctggacgac gctgtgggcg gtaaaggagt ccagtccgga
     8641 ggtgggctca tcgcagatca gaagcggcgg atcggttaga gcctcggagg cgaatgccag
     8701 acgcttcctt tctccgccgg acagaccttt caccctgccg ggcacaccga tgatcgtgtg
     8761 ctgacatttg ctgagcgaaa gctcctggat cacctgatcc acgcgggcca ctcgctgccg
     8821 ataggtcaga tgtcgtggca tccgcaccat ggcttggaaa atcaggtgtt ccctggccgt
     8881 tagggagccg ataaagaggt catcctgctg gacataggcg cacctggcct gcatctcctt
     8941 ggcgtccaca ggttggccat tgagcagtcg catcccggat ggcgatactt ggatgccctg
     9001 cggcgatcga aaggcaaggg cattcagcag ggtcgtcttt ccggcaccgg aactgcccat
     9061 cacggccaaa agttcgcccg gataggccac gccgcaaact gagtttcaaa ttggtaattg
     9121 gaccctttat taagatttca cacagatcag ccgactgcga atagaaactc accgttcttg
     9181 agcaaatgtt tcctgggcgc cggtatgtgt cgctcgttgc agaatagtcc gcgtgtccgg
     9241 ttgaccagct gccgccatcc ggagcccggc tgattgaccg ccccaaagat gtccatattg
     9301 tgccaggcat aggtgaggtt ctcggctagt tggccgctcc ctgaaccgga gtcctccggc
     9361 ggactgggtg gccggagcgt gccgtagttt ttggcctgcc cgaagccctg gttaatgcag
     9421 ctctgcgaag ccgctccgct gtcaccctgc aatgataggg gatctcaaat atcaactaca
     9481 agcgttatgc tcatctaacc ccgaacaaaa agtaccccga agtatcctac gaagtaggtt
     9541 tatactttta tttatttttt gtgcatctag gatcagctta aaatatctgg ttgttatatt
     9601 ttttgtaaaa aagaatatag tcgaaaatga atgcctttag atgtcttgat catgatatga
     9661 tctcaaaaat tgtcttatat agcgagaaca gctaccagaa taatctgttt cgtgtcacta
     9721 tttgtttgtg caattgcggt ttgggatttt tgtgggtcgc agttctcacg ccgcagacaa
     9781 tttgatgttg caatcgcagt tcctatagat caagtgaact taagatgtat gcacatgtac
     9841 tactcacatt gttcagatgc tcggcagatg ggtgtttgct gcctccgcga attaatagct
     9901 cctgatcctc ttggcccatt gccgggattt ttcacacttt cccctgctta cccacccaaa
     9961 accaatcacc accccaatca ctcaaaaaac aaacaaaaat aagaagcgag aggagttttg
    10021 gcacagcact ttgtgtttaa ttgatggcgt aaaccgcttg gagcttcgtc acgaaaccgc
    10081 tgacaaaatg caactgaagg cgga
//
