LOCUS       sequence-320095-       10253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This insert, when expressed in the same cell with other split Cre 
            component, forms Cre recombinase through protein trans-splicing 
            using split intein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Luan H, Kuzin A, Odenwald WF, White BH
  TITLE     Cre-assisted fine-mapping of neural circuits using orthogonal split 
            inteins.
  JOURNAL   Elife. 2020 Apr 14;9. pii: 53041. doi: 10.7554/eLife.53041.
  PUBMED    32286225
REFERENCE   2  (bases 1 to 10253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-04-14"; volume: "9. pii: 53041. doi"; pages: " 
            10.7554/eLife.53041"
COMMENT     Sequence Label: HJP-207-IVS-Syn21-gp41-1C-CreBC-WPREUw
FEATURES             Location/Qualifiers
     source          1..10253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..125
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(150..155,156..173,174..180)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             234..893
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(300..319)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1235..1540
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1419..1438
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1581..1705)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          1893..1959
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     misc_feature    2818..3406
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2871..2891)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3289..3300)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3667..3683)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3667..3683)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(4694..5308,5379..5510,5714..6029,6091..6745,
                     6820..7093,7495..7566))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     8018..8036
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    8261..8330
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(8417..9005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8497..8516)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9176..9967,9968..10036))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9799..9818
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10037..10141)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10208..10226
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
       61 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      121 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
      181 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
      241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      361 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      421 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      481 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      541 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      601 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      661 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
      721 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
      781 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
      841 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
      901 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
      961 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     1021 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1081 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1141 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     1201 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     1261 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     1321 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     1381 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     1441 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     1501 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     1561 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     1621 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     1681 gttcagcttt cttgtacaaa gtggtgataa acggccggcc gagctcgccc ggggatcgag
     1741 cgcagcggta taaaagggcg cggggtggct gagagcatca gttgtgaatg aatgttcgag
     1801 ccgagcagac gtgccgctgc cttcgttaat atcctttgaa taagccaact ttgaatcaca
     1861 agacgcatac caaacggtac cgctagaaaa aggtaggttc aaccactgat gcctaggcac
     1921 accgaaacga ctaaccctaa ttcttatcct ttacttcagg gctagcaact taaaaaaaaa
     1981 aatcaaaatg atgctgaaga agatcctgaa gatcgaggag ctggatgagc gcgagctgat
     2041 cgatatcgag gtgagcggca accacctgtt ctacgccaac gatatcctga cccacaacag
     2101 caatgcagtg agcctggtaa tgcgtcgaat acgcaaggaa aacgtggacg ccggcgagag
     2161 ggccaaacag gcgttggctt tcgaacgcac cgatttcgat caggtgcggt cgttgatgga
     2221 gaacagcgat cgatgccagg acatccggaa cttggcgttt ttgggcattg cctataacac
     2281 cctccttcgc atcgccgaga tagcgcgtat ccgagttaaa gacatttccc gtacagacgg
     2341 aggccgcatg cttattcaca tcggacgcac gaaaaccctg gtcagcactg ctggcgtgga
     2401 gaaagcactt tccctgggtg tgacgaaatt ggtcgagcga tggatcagtg tgagtggcgt
     2461 cgccgatgac cccaacaatt atctgttctg cagggtgcgc aaaaatggag tggcagcccc
     2521 ctcggcaaca agtcagttga gtacgcgcgc gttggaggga atcttcgagg ccactcaccg
     2581 attgatttac ggtgcaaaag atgactccgg ccaacgttac ttggcctggt ccggtcacag
     2641 cgcacgtgtg ggtgccgccc gtgacatggc gagggccggt gtttccatcc cagaaatcat
     2701 gcaagcgggc ggatggacca acgtgaatat tgtaatgaac tacattcgta atctggatag
     2761 tgaaacaggc gcaatggtgc gcctgctgga ggacggagat taaaccggtg tttaaacaat
     2821 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2881 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2941 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3001 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3061 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3121 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3181 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3241 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3301 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3361 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga agcttatcga
     3421 taccgtcgac taaagccaaa tagaaaatta ttcagttcct ggcttaagtt tttaaaagtg
     3481 atattattta tttggttgta accaaccaaa agaatgtaaa taactaatac ataattatgt
     3541 tagttttaag ttagcaacaa attgatttta gctatattag ctacttggtt aataaataga
     3601 atatatttat ttaaagataa ttgcgttttt attgtcaggg agtgagtttg cttaaaaact
     3661 cgtttagatc cactagttct agagcggccg ctggccacgg gtgcgcatga tcgtgctcct
     3721 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc
     3781 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac
     3841 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg
     3901 caccattatg ttccggaggc gcgccctagt tccagtgaaa tccaagcatt ttctaaatta
     3961 aatgtattct tattattata gttgttattt ttgatatata taaacaacac tattatgccc
     4021 accatttttt tgagatgcat ctacacaagg aacaaacact ggatgtcact ttcagttcaa
     4081 attgtaacgc taatcactcc gaacaggtca caaaaaatta ccttaaaaag tcataatatt
     4141 aaattagaat aaatatagct gtgagggaaa tatatacaaa tatattggag caaataaatt
     4201 gtacatacaa atatttatta ctaatttcta ttgagacgaa atgaaccact cggaaccatt
     4261 tgagcgaacc gaatcgcgcg gaactaacga cagtcgctcc aaggtcgtcg aacaaaaggt
     4321 gaatgtgttg cggagagcgg gtgggagaca gcgaaagagc aactacgaaa cgtggtgtgg
     4381 tggaggtgaa ttatgaagag ggcgcgcgat ttgaaaagta tgtatataaa aaatatatcc
     4441 cggtgtttta tgtagcgata aacgagtttt tgatgtaagg tatgcaggtg tgtaagtctt
     4501 ttggttagaa gacaaatcca aagtctactt gtggggatgt tcgaagggga aatacttgta
     4561 ttctataggt catatcttgt ttttattggc acaaatataa ttacattagc tttttgaggg
     4621 ggcaataaac agtaaacacg atggtaataa tggtaaaaaa aaaaaacaag cagttatttc
     4681 ggatatatgt cggctactcc ttgcgtcggg cccgaagtct tagagccaga tatgcgagca
     4741 cccggaagct cacgatgaga atggccagac ccacgtagtc cagcggcaga tcggcggcgg
     4801 agaagttaag cgtctccagg atgaccttgc ccgaactggg gcacgtggtg ttcgacgatg
     4861 tgcagctaat ttcgcccggc tccacgtccg cccattggtt aatcagcaga ccctcgttgg
     4921 cgtaacggaa ccatgagagg tacgacaacc atttgaggta tactggcacc gagcccgagt
     4981 tcaagaagaa gccgccaaag agcaggaatg gtatgataac cggcggaccc acagacagcg
     5041 ccatcgaggt cgaggagctg gcgcaggata ttagatatcc gaaggacgtt gacacattgg
     5101 ccaccagagt gaccagcgcc aggcagttga agaagtgcag cactccggcc cgcagtccga
     5161 tcatcggata ggcaatcgcc gtgaagacca gtggcactgt gagaaaaagc ggcaattcgg
     5221 caatcgtttt gcccagaaag tatgtgtcac agcgataaag tcgacttcgg gcctccctca
     5281 taaaaactgg cagctctgag gtgaacacct aaatcgaatc gattcattag aaagttagta
     5341 aattattgaa atgcaaatgt attctaaaca tgacttacat ttatcgtggc aaagacgttt
     5401 tgaaaggtca tgttggtcag gaagaggaag atggctccgt tgatattcat cacacccact
     5461 tgcgtgagtt gttggcccaa aaagatgagg ccaatcaaga tggcaaccat ctgcaaatta
     5521 aaatgttact cgcatctcat taatattcgc gagttaaatg aaatttattt atcttctgca
     5581 aaactataaa ctatacatct cattgaaaaa aactaagaag ggtgtggaat caggcaattc
     5641 tatctaaaat ctagcgaatt tgtttccaag aattgtaagc gttatatcat ttgtttccac
     5701 tggaaccact caccgttgtc tgaataagtc gcacttttac gaggagtggt tccttgagca
     5761 ccgacagcca ggatcgccac aggaccgccc ggaactgcat gaaccaggtg gccttgtagg
     5821 tgtacccatt ctccggctgc tccagtggct tctccagatt tttggtggcc aacaactgct
     5881 ccatatcccg ggctactttg ctaatggcaa aattgtcgca tatcttggcg atccgatcac
     5941 gggactcgat ctcccgtccg ggcacaacgg ccaacacctg tacgtaaaag tccgccggat
     6001 tgtagttggt aggacactgg gcacccacgc tggataggag ttgagatgtt atgtaatact
     6061 agataccctt aataaacaca tcgaactcac taggaaaaga agtcgacggc ttcgctggga
     6121 gtgcccaaga aagctaccct gccctcggcc atcagaagga tcttgtcaaa gagctcaaac
     6181 agctcggaag acggctgatg aatggtcagg atgacggtct tgcccttctg cgacagcttc
     6241 ttcagcacct ggacgacgct gtgggcggta aaggagtcca gtccggaggt gggctcatcg
     6301 cagatcagaa gcggcggatc ggttagagcc tcggaggcga atgccagacg cttcctttct
     6361 ccgccggaca gacctttcac cctgccgggc acaccgatga tcgtgtgctg acatttgctg
     6421 agcgaaagct cctggatcac ctgatccacg cgggccactc gctgccgata ggtcagatgt
     6481 cgtggcatcc gcaccatggc ttggaaaatc aggtgttccc tggccgttag ggagccgata
     6541 aagaggtcat cctgctggac ataggcgcac ctggcctgca tctccttggc gtccacaggt
     6601 tggccattga gcagtcgcat cccggatggc gatacttgga tgccctgcgg cgatcgaaag
     6661 gcaagggcat tcagcagggt cgtctttccg gcaccggaac tgcccatcac ggccaaaagt
     6721 tcgcccggat aggccacgcc gcaaactgag tttcaaattg gtaattggac cctttattaa
     6781 gatttcacac agatcagccg actgcgaata gaaactcacc gttcttgagc aaatgtttcc
     6841 tgggcgccgg tatgtgtcgc tcgttgcaga atagtccgcg tgtccggttg accagctgcc
     6901 gccatccgga gcccggctga ttgaccgccc caaagatgtc catattgtgc caggcatagg
     6961 tgaggttctc ggctagttgg ccgctccctg aaccggagtc ctccggcgga ctgggtggcc
     7021 ggagcgtgcc gtagtttttg gcctgcccga agccctggtt aatgcagctc tgcgaagccg
     7081 ctccgctgtc accctgcaat gataggggat ctcaaatatc aactacaagc gttatgctca
     7141 tctaaccccg aacaaaaagt accccgaagt atcctacgaa gtaggtttat acttttattt
     7201 attttttgtg catctaggat cagcttaaaa tatctggttg ttatattttt tgtaaaaaag
     7261 aatatagtcg aaaatgaatg cctttagatg tcttgatcat gatatgatct caaaaattgt
     7321 cttatatagc gagaacagct accagaataa tctgtttcgt gtcactattt gtttgtgcaa
     7381 ttgcggtttg ggatttttgt gggtcgcagt tctcacgccg cagacaattt gatgttgcaa
     7441 tcgcagttcc tatagatcaa gtgaacttaa gatgtatgca catgtactac tcacattgtt
     7501 cagatgctcg gcagatgggt gtttgctgcc tccgcgaatt aatagctcct gatcctcttg
     7561 gcccattgcc gggatttttc acactttccc ctgcttaccc acccaaaacc aatcaccacc
     7621 ccaatcactc aaaaaacaaa caaaaataag aagcgagagg agttttggca cagcactttg
     7681 tgtttaattg atggcgtaaa ccgcttggag cttcgtcacg aaaccgctga caaaatgcaa
     7741 ctgaaggcgg acattgacgc tacgtaacgc tacaaacggt ggcgaaagag atagcggacg
     7801 cagcggcgaa agagacggcg atatttctgt ggacagagaa ggaggcaaac agcgctgact
     7861 ttgagtggaa tgtcattttg agtgagaggt aatcgaaaga acctggtaca tcaaataccc
     7921 ttggatcgaa gtaaatttaa aactgatcag ataagttcaa tgatatccag tgcagtaaaa
     7981 aaaaaaaatg ttttttttat ctactttccg caaaaatggg ttttattaac ttacatacat
     8041 actaggcgcg cccatatgtt cggcttgtcg acatgcccgc cgtgaccgtc gagaacccgc
     8101 tgacgctgcc ccgcgtatcc gcacccgccg acgccgtcgc acgtcccgtg ctcaccgtga
     8161 ccaccgcgcc cagcggtttc gagggcgagg gcttcccggt gcgccgcgcg ttcgccggga
     8221 tcaactaccg ccacctcgac ccgttcatca tgatggacca gatgggtgag gtggagtacg
     8281 cgcccgggga gcccaagggc acgccctggc acccgcaccg cggcttcgag accgtgacct
     8341 acatcgtcga cggtaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8401 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8461 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8521 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8581 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8641 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8701 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8761 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8821 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8881 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8941 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9001 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9061 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9121 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9181 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9241 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9301 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9361 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9421 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9481 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9541 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9601 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9661 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9721 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9781 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9841 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9901 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9961 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10021 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10081 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    10141 gcacatttcc ccgaaaagtg ccacctgagt ctaagaaacc attattatca tgacattaac
    10201 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattcgttt atc
//
