LOCUS       sequence-320097-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MAT2A in E. coli BL21(DE3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Firestone RS, Schramm VL
  TITLE     The Transition-State Structure for Human MAT2A from Isotope Effects.
  JOURNAL   J Am Chem Soc. 2017 Oct 4;139(39):13754-13760. doi: 
            10.1021/jacs.7b05803. Epub 2017 Sep 20.
  PUBMED    28880543
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.7b05803"; journalName: "J Am Chem Soc"; date: 
            "2017-10-4- 4"; volume: "139"; issue: "39"; pages: "13754-13760"
COMMENT     Sequence Label: pET-MAT2A
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             91..111
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttnnnnctan natnntttgt ttaactttaa gaaggagata tacatatgca ccatcatcat
       61 catcattctt ctggtgtaga tctgggtacc gagaacctgt acttccaatc catgaacgga
      121 cagctcaacg gcttccatga ggcgttcatc gaggagggca cattcctttt cacctcagag
      181 tcggtcgggg aaggccaccc agataagatt tgtgaccaaa tcagtgatgc tgtccttgat
      241 gcccaccttc agcaggatcc tgatgccaaa gtagcttgtg aaactgttgc taaaactgga
      301 atgatccttc ttgctgggga aattacatcc agagctgctg ttgactacca gaaagtggtt
      361 cgtgaagctg ttaaacacat tggatatgat gattcttcca aaggttttga ctacaagact
      421 tgtaacgtgc tggtagcctt ggagcaacag tcaccagata ttgctcaagg tgttcatctt
      481 gacagaaatg aagaagacat tggtgctgga gaccagggct taatgtttgg ctatgccact
      541 gatgaaactg aggagtgtat gcctttaacc attgtcttgg cacacaagct aaatgccaaa
      601 ctggcagaac tacgccgtaa tggcactttg ccttggttac gccctgattc taaaactcaa
      661 gttactgtgc agtatatgca ggatcgaggt gctgtgcttc ccatcagagt ccacacaatt
      721 gttatatctg ttcagcatga tgaagaggtt tgtcttgatg aaatgaggga tgccctaaag
      781 gagaaagtca tcaaagcagt tgtgcctgcg aaataccttg atgaggatac aatctaccac
      841 ctacagccaa gtggcagatt tgttattggt gggcctcagg gtgatgctgg tttgactggn
      901 cgcaaaatca ttgtggannn cttatggngg ttggggtgnn ncat
//
