LOCUS       sequence-320098-       10140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This insert, when expressed in the same cell with other split Cre 
            component, forms Cre recombinase through protein trans-splicing 
            using split intein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Luan H, Kuzin A, Odenwald WF, White BH
  TITLE     Cre-assisted fine-mapping of neural circuits using orthogonal split 
            inteins.
  JOURNAL   Elife. 2020 Apr 14;9. pii: 53041. doi: 10.7554/eLife.53041.
  PUBMED    32286225
REFERENCE   2  (bases 1 to 10140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-04-14"; volume: "9. pii: 53041. doi"; pages: " 
            10.7554/eLife.53041"
COMMENT     Sequence Label: HJP-180-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREw
FEATURES             Location/Qualifiers
     source          1..10140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     326..344
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    569..638
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(725..1313)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(805..824)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1484..2275,2276..2344))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2107..2126
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2345..2449)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2516..2534
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    2562..2686
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2711..2716,2717..2734,2735..2741)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2795..3454
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2861..2880)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3796..4101
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3980..3999
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4142..4266)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          4305..4371
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     misc_feature    5266..5854
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5319..5339)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5737..5748)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6115..6131)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6115..6131)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(7142..7756,7827..7958,8162..8477,8539..9193,
                     9268..9541,9943..10014))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 ggcgtaaacc gcttggagct tcgtcacgaa accgctgaca aaatgcaact gaaggcggac
       61 attgacgcta cgtaacgcta caaacggtgg cgaaagagat agcggacgca gcggcgaaag
      121 agacggcgat atttctgtgg acagagaagg aggcaaacag cgctgacttt gagtggaatg
      181 tcattttgag tgagaggtaa tcgaaagaac ctggtacatc aaataccctt ggatcgaagt
      241 aaatttaaaa ctgatcagat aagttcaatg atatccagtg cagtaaaaaa aaaaaatgtt
      301 ttttttatct actttccgca aaaatgggtt ttattaactt acatacatac taggcgcgcc
      361 catatgttcg gcttgtcgac atgcccgccg tgaccgtcga gaacccgctg acgctgcccc
      421 gcgtatccgc acccgccgac gccgtcgcac gtcccgtgct caccgtgacc accgcgccca
      481 gcggtttcga gggcgagggc ttcccggtgc gccgcgcgtt cgccgggatc aactaccgcc
      541 acctcgaccc gttcatcatg atggaccaga tgggtgaggt ggagtacgcg cccggggagc
      601 ccaagggcac gccctggcac ccgcaccgcg gcttcgagac cgtgacctac atcgtcgacg
      661 gtaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      721 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      781 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      841 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      901 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      961 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1021 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1081 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1141 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1201 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1261 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1321 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1381 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1441 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     1501 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1561 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1621 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1681 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     1741 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     1801 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     1861 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     1921 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1981 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2041 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2101 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2161 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2221 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2281 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2341 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2401 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     2461 gaaaagtgcc acctgagtct aagaaaccat tattatcatg acattaacct ataaaaatag
     2521 gcgtatcacg aggccctttc gtcttcaaga attcgtttat cacaagtttg tacaaaaaag
     2581 ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
     2641 aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg
     2701 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     2761 tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     2821 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     2881 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     2941 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     3001 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     3061 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     3121 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     3181 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     3241 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     3301 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     3361 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     3421 acagtactgc gatgagtggc agggcggggc gtaaacgcgt ggatccggct tactaaaagc
     3481 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     3541 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt
     3601 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     3661 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     3721 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     3781 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     3841 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
     3901 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     3961 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
     4021 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     4081 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
     4141 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     4201 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
     4261 agtggtgata aacggccggc cagatctggt accgctagaa aaaggtaggt tcaaccactg
     4321 atgcctaggc acaccgaaac gactaaccct aattcttatc ctttacttca gggctagcaa
     4381 cttaaaaaaa aaaatcaaaa tgatgctgaa gaagatcctg aagatcgagg agctggatga
     4441 gcgcgagctg atcgatatcg aggtgagcgg caaccacctg ttctacgcca acgatatcct
     4501 gacccacaac agcaatgcag tgagcctggt aatgcgtcga atacgcaagg aaaacgtgga
     4561 cgccggcgag agggccaaac aggcgttggc tttcgaacgc accgatttcg atcaggtgcg
     4621 gtcgttgatg gagaacagcg atcgatgcca ggacatccgg aacttggcgt ttttgggcat
     4681 tgcctataac accctccttc gcatcgccga gatagcgcgt atccgagtta aagacatttc
     4741 ccgtacagac ggaggccgca tgcttattca catcggacgc acgaaaaccc tggtcagcac
     4801 tgctggcgtg gagaaagcac tttccctggg tgtgacgaaa ttggtcgagc gatggatcag
     4861 tgtgagtggc gtcgccgatg accccaacaa ttatctgttc tgcagggtgc gcaaaaatgg
     4921 agtggcagcc ccctgcctgg tgggcagcag cgagatcatc acccgcaact acggcaagac
     4981 caccatcaag gaggtggtgg agatcttcga taacgataag aacatccagg tgctggcctt
     5041 caacacccac accgataaca tcgagtgggc ccccatcaag gccgcccagc tgacccgccc
     5101 caacgccgag ctggtggagc tggagatcga taccctgcac ggcgtgaaga ccatccgctg
     5161 cacccccgat caccccgtgt acaccaagaa ccgcggctac gtgcgcgccg atgagctgac
     5221 cgatgatgat gagctggtgg tggccatcta aaccggtgtt taaacaatca acctctggat
     5281 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5341 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5401 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5461 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5521 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5581 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5641 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     5701 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     5761 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     5821 acgagtcgga tctccctttg ggccgcctcc ccgcctgaag cttatcgata ccgtcgacta
     5881 aagccaaata gaaaattatt cagttcctgg cttaagtttt taaaagtgat attatttatt
     5941 tggttgtaac caaccaaaag aatgtaaata actaatacat aattatgtta gttttaagtt
     6001 agcaacaaat tgattttagc tatattagct acttggttaa taaatagaat atatttattt
     6061 aaagataatt gcgtttttat tgtcagggag tgagtttgct taaaaactcg tttagatcca
     6121 ctagttctag agcggccgct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac
     6181 ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg
     6241 aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt
     6301 cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt
     6361 ccggaggcgc gccctagttc cagtgaaatc caagcatttt ctaaattaaa tgtattctta
     6421 ttattatagt tgttattttt gatatatata aacaacacta ttatgcccac catttttttg
     6481 agatgcatct acacaaggaa caaacactgg atgtcacttt cagttcaaat tgtaacgcta
     6541 atcactccga acaggtcaca aaaaattacc ttaaaaagtc ataatattaa attagaataa
     6601 atatagctgt gagggaaata tatacaaata tattggagca aataaattgt acatacaaat
     6661 atttattact aatttctatt gagacgaaat gaaccactcg gaaccatttg agcgaaccga
     6721 atcgcgcgga actaacgaca gtcgctccaa ggtcgtcgaa caaaaggtga atgtgttgcg
     6781 gagagcgggt gggagacagc gaaagagcaa ctacgaaacg tggtgtggtg gaggtgaatt
     6841 atgaagaggg cgcgcgattt gaaaagtatg tatataaaaa atatatcccg gtgttttatg
     6901 tagcgataaa cgagtttttg atgtaaggta tgcaggtgtg taagtctttt ggttagaaga
     6961 caaatccaaa gtctacttgt ggggatgttc gaaggggaaa tacttgtatt ctataggtca
     7021 tatcttgttt ttattggcac aaatataatt acattagctt tttgaggggg caataaacag
     7081 taaacacgat ggtaataatg gtaaaaaaaa aaaacaagca gttatttcgg atatatgtcg
     7141 gctactcctt gcgtcgggcc cgaagtctta gagccagata tgcgagcacc cggaagctca
     7201 cgatgagaat ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg
     7261 tctccaggat gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt
     7321 cgcccggctc cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc
     7381 atgagaggta cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagc
     7441 cgccaaagag caggaatggt atgataaccg gcggacccac agacagcgcc atcgaggtcg
     7501 aggagctggc gcaggatatt agatatccga aggacgttga cacattggcc accagagtga
     7561 ccagcgccag gcagttgaag aagtgcagca ctccggcccg cagtccgatc atcggatagg
     7621 caatcgccgt gaagaccagt ggcactgtga gaaaaagcgg caattcggca atcgttttgc
     7681 ccagaaagta tgtgtcacag cgataaagtc gacttcgggc ctccctcata aaaactggca
     7741 gctctgaggt gaacacctaa atcgaatcga ttcattagaa agttagtaaa ttattgaaat
     7801 gcaaatgtat tctaaacatg acttacattt atcgtggcaa agacgttttg aaaggtcatg
     7861 ttggtcagga agaggaagat ggctccgttg atattcatca cacccacttg cgtgagttgt
     7921 tggcccaaaa agatgaggcc aatcaagatg gcaaccatct gcaaattaaa atgttactcg
     7981 catctcatta atattcgcga gttaaatgaa atttatttat cttctgcaaa actataaact
     8041 atacatctca ttgaaaaaaa ctaagaaggg tgtggaatca ggcaattcta tctaaaatct
     8101 agcgaatttg tttccaagaa ttgtaagcgt tatatcattt gtttccactg gaaccactca
     8161 ccgttgtctg aataagtcgc acttttacga ggagtggttc cttgagcacc gacagccagg
     8221 atcgccacag gaccgcccgg aactgcatga accaggtggc cttgtaggtg tacccattct
     8281 ccggctgctc cagtggcttc tccagatttt tggtggccaa caactgctcc atatcccggg
     8341 ctactttgct aatggcaaaa ttgtcgcata tcttggcgat ccgatcacgg gactcgatct
     8401 cccgtccggg cacaacggcc aacacctgta cgtaaaagtc cgccggattg tagttggtag
     8461 gacactgggc acccacgctg gataggagtt gagatgttat gtaatactag atacccttaa
     8521 taaacacatc gaactcacta ggaaaagaag tcgacggctt cgctgggagt gcccaagaaa
     8581 gctaccctgc cctcggccat cagaaggatc ttgtcaaaga gctcaaacag ctcggaagac
     8641 ggctgatgaa tggtcaggat gacggtcttg cccttctgcg acagcttctt cagcacctgg
     8701 acgacgctgt gggcggtaaa ggagtccagt ccggaggtgg gctcatcgca gatcagaagc
     8761 ggcggatcgg ttagagcctc ggaggcgaat gccagacgct tcctttctcc gccggacaga
     8821 cctttcaccc tgccgggcac accgatgatc gtgtgctgac atttgctgag cgaaagctcc
     8881 tggatcacct gatccacgcg ggccactcgc tgccgatagg tcagatgtcg tggcatccgc
     8941 accatggctt ggaaaatcag gtgttccctg gccgttaggg agccgataaa gaggtcatcc
     9001 tgctggacat aggcgcacct ggcctgcatc tccttggcgt ccacaggttg gccattgagc
     9061 agtcgcatcc cggatggcga tacttggatg ccctgcggcg atcgaaaggc aagggcattc
     9121 agcagggtcg tctttccggc accggaactg cccatcacgg ccaaaagttc gcccggatag
     9181 gccacgccgc aaactgagtt tcaaattggt aattggaccc tttattaaga tttcacacag
     9241 atcagccgac tgcgaataga aactcaccgt tcttgagcaa atgtttcctg ggcgccggta
     9301 tgtgtcgctc gttgcagaat agtccgcgtg tccggttgac cagctgccgc catccggagc
     9361 ccggctgatt gaccgcccca aagatgtcca tattgtgcca ggcataggtg aggttctcgg
     9421 ctagttggcc gctccctgaa ccggagtcct ccggcggact gggtggccgg agcgtgccgt
     9481 agtttttggc ctgcccgaag ccctggttaa tgcagctctg cgaagccgct ccgctgtcac
     9541 cctgcaatga taggggatct caaatatcaa ctacaagcgt tatgctcatc taaccccgaa
     9601 caaaaagtac cccgaagtat cctacgaagt aggtttatac ttttatttat tttttgtgca
     9661 tctaggatca gcttaaaata tctggttgtt atattttttg taaaaaagaa tatagtcgaa
     9721 aatgaatgcc tttagatgtc ttgatcatga tatgatctca aaaattgtct tatatagcga
     9781 gaacagctac cagaataatc tgtttcgtgt cactatttgt ttgtgcaatt gcggtttggg
     9841 atttttgtgg gtcgcagttc tcacgccgca gacaatttga tgttgcaatc gcagttccta
     9901 tagatcaagt gaacttaaga tgtatgcaca tgtactactc acattgttca gatgctcggc
     9961 agatgggtgt ttgctgcctc cgcgaattaa tagctcctga tcctcttggc ccattgccgg
    10021 gatttttcac actttcccct gcttacccac ccaaaaccaa tcaccacccc aatcactcaa
    10081 aaaacaaaca aaaataagaa gcgagaggag ttttggcaca gcactttgtg tttaattgat
//
