LOCUS       sequence-320099-        9839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This insert, when expressed in the same cell with other split Cre 
            component, forms Cre recombinase through protein trans-splicing 
            using split intein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Luan H, Kuzin A, Odenwald WF, White BH
  TITLE     Cre-assisted fine-mapping of neural circuits using orthogonal split 
            inteins.
  JOURNAL   Elife. 2020 Apr 14;9. pii: 53041. doi: 10.7554/eLife.53041.
  PUBMED    32286225
REFERENCE   2  (bases 1 to 9839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-04-14"; volume: "9. pii: 53041. doi"; pages: " 
            10.7554/eLife.53041"
COMMENT     Sequence Label: HJP-196-IVS-Syn21-NrdJ-1C-CreC-WPREUw
FEATURES             Location/Qualifiers
     source          1..9839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(92..110)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(562..633,1035..1308,1383..2037,2099..2414,2618..2749,
                     2820..3434)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4445..4461
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4445..4461
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    4722..5310
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4828..4839
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5237..5257
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     intron          5755..5821
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     protein_bind    6009..6133
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(6174..6479)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(6276..6295)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(6821..7480)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7395..7414
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(7534..7540,7541..7558,7559..7564))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(7589..7713)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7741..7759)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7826..7930
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7931..7999,8000..8791)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8149..8168)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8962..9550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9451..9470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    9637..9706
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 cgggtgcgga tacgcggggc agcgtcagcg ggttctcgac ggtcacggcg ggcatgtcga
       61 caagccgaac atatgggcgc gcctagtatg tatgtaagtt aataaaaccc atttttgcgg
      121 aaagtagata aaaaaaacat tttttttttt tactgcactg gatatcattg aacttatctg
      181 atcagtttta aatttacttc gatccaaggg tatttgatgt accaggttct ttcgattacc
      241 tctcactcaa aatgacattc cactcaaagt cagcgctgtt tgcctccttc tctgtccaca
      301 gaaatatcgc cgtctctttc gccgctgcgt ccgctatctc tttcgccacc gtttgtagcg
      361 ttacgtagcg tcaatgtccg ccttcagttg cattttgtca gcggtttcgt gacgaagctc
      421 caagcggttt acgccatcaa ttaaacacaa agtgctgtgc caaaactcct ctcgcttctt
      481 atttttgttt gttttttgag tgattggggt ggtgattggt tttgggtggg taagcagggg
      541 aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg agctattaat tcgcggaggc
      601 agcaaacacc catctgccga gcatctgaac aatgtgagta gtacatgtgc atacatctta
      661 agttcacttg atctatagga actgcgattg caacatcaaa ttgtctgcgg cgtgagaact
      721 gcgacccaca aaaatcccaa accgcaattg cacaaacaaa tagtgacacg aaacagatta
      781 ttctggtagc tgttctcgct atataagaca atttttgaga tcatatcatg atcaagacat
      841 ctaaaggcat tcattttcga ctatattctt ttttacaaaa aatataacaa ccagatattt
      901 taagctgatc ctagatgcac aaaaaataaa taaaagtata aacctacttc gtaggatact
      961 tcggggtact ttttgttcgg ggttagatga gcataacgct tgtagttgat atttgagatc
     1021 ccctatcatt gcagggtgac agcggagcgg cttcgcagag ctgcattaac cagggcttcg
     1081 ggcaggccaa aaactacggc acgctccggc cacccagtcc gccggaggac tccggttcag
     1141 ggagcggcca actagccgag aacctcacct atgcctggca caatatggac atctttgggg
     1201 cggtcaatca gccgggctcc ggatggcggc agctggtcaa ccggacacgc ggactattct
     1261 gcaacgagcg acacataccg gcgcccagga aacatttgct caagaacggt gagtttctat
     1321 tcgcagtcgg ctgatctgtg tgaaatctta ataaagggtc caattaccaa tttgaaactc
     1381 agtttgcggc gtggcctatc cgggcgaact tttggccgtg atgggcagtt ccggtgccgg
     1441 aaagacgacc ctgctgaatg cccttgcctt tcgatcgccg cagggcatcc aagtatcgcc
     1501 atccgggatg cgactgctca atggccaacc tgtggacgcc aaggagatgc aggccaggtg
     1561 cgcctatgtc cagcaggatg acctctttat cggctcccta acggccaggg aacacctgat
     1621 tttccaagcc atggtgcgga tgccacgaca tctgacctat cggcagcgag tggcccgcgt
     1681 ggatcaggtg atccaggagc tttcgctcag caaatgtcag cacacgatca tcggtgtgcc
     1741 cggcagggtg aaaggtctgt ccggcggaga aaggaagcgt ctggcattcg cctccgaggc
     1801 tctaaccgat ccgccgcttc tgatctgcga tgagcccacc tccggactgg actcctttac
     1861 cgcccacagc gtcgtccagg tgctgaagaa gctgtcgcag aagggcaaga ccgtcatcct
     1921 gaccattcat cagccgtctt ccgagctgtt tgagctcttt gacaagatcc ttctgatggc
     1981 cgagggcagg gtagctttct tgggcactcc cagcgaagcc gtcgacttct tttcctagtg
     2041 agttcgatgt gtttattaag ggtatctagt attacataac atctcaactc ctatccagcg
     2101 tgggtgccca gtgtcctacc aactacaatc cggcggactt ttacgtacag gtgttggccg
     2161 ttgtgcccgg acgggagatc gagtcccgtg atcggatcgc caagatatgc gacaattttg
     2221 ccattagcaa agtagcccgg gatatggagc agttgttggc caccaaaaat ctggagaagc
     2281 cactggagca gccggagaat gggtacacct acaaggccac ctggttcatg cagttccggg
     2341 cggtcctgtg gcgatcctgg ctgtcggtgc tcaaggaacc actcctcgta aaagtgcgac
     2401 ttattcagac aacggtgagt ggttccagtg gaaacaaatg atataacgct tacaattctt
     2461 ggaaacaaat tcgctagatt ttagatagaa ttgcctgatt ccacaccctt cttagttttt
     2521 ttcaatgaga tgtatagttt atagttttgc agaagataaa taaatttcat ttaactcgcg
     2581 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     2641 catctttttg ggccaacaac tcacgcaagt gggtgtgatg aatatcaacg gagccatctt
     2701 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcatgt
     2761 ttagaataca tttgcatttc aataatttac taactttcta atgaatcgat tcgatttagg
     2821 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     2881 acacatactt tctgggcaaa acgattgccg aattgccgct ttttctcaca gtgccactgg
     2941 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3001 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3061 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3121 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3181 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3241 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3301 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3361 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     3421 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     3481 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     3541 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     3601 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     3661 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     3721 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     3781 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     3841 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     3901 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     3961 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4021 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4081 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4141 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4201 agggcgcgcc tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac
     4261 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag
     4321 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc
     4381 cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccagcgg
     4441 ccgctctaga actagtggat ctaaacgagt ttttaagcaa actcactccc tgacaataaa
     4501 aacgcaatta tctttaaata aatatattct atttattaac caagtagcta atatagctaa
     4561 aatcaatttg ttgctaactt aaaactaaca taattatgta ttagttattt acattctttt
     4621 ggttggttac aaccaaataa ataatatcac ttttaaaaac ttaagccagg aactgaataa
     4681 ttttctattt ggctttagtc gacggtatcg ataagcttca ggcggggagg cggcccaaag
     4741 ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag
     4801 caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt
     4861 cccgcgcaga atccaggtgg caacacaggc gagcagccaa ggaaaggacg atgatttccc
     4921 cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag
     4981 gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag
     5041 gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca
     5101 caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga
     5161 tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat
     5221 taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct
     5281 ttcacaaatt ttgtaatcca gaggttgatt gtttaaacac cggtttaatc tccgtcctcc
     5341 agcaggcgca ccattgcgcc tgtttcacta tccagattac gaatgtagtt cattacaata
     5401 ttcacgttgg tccatccgcc cgcttgcatg atttctggga tggaaacacc ggccctcgcc
     5461 atgtcacggg cggcacccac acgtgcgctg tgaccggacc aggccaagta acgttggccg
     5521 gagtcatctt ttgcaccgta aatcaatcgg tgagtggcct cgaagattcc ctccaacgcg
     5581 cgcgtactca actgacttgt tgccgagttg tgcaccagga tatcgttggc gaagaagttg
     5641 tgggtgctgg tctggatatc gtaggtatcc tcgttgctca cgatcttgcg gctcttcagc
     5701 ttgccgatgt aggtcttggc ctccattttg attttttttt ttaagttgct agccctgaag
     5761 taaaggataa gaattagggt tagtcgtttc ggtgtgccta ggcatcagtg gttgaaccta
     5821 cctttttcta gcggtaccgt ttggtatgcg tcttgtgatt caaagttggc ttattcaaag
     5881 gatattaacg aaggcagcgg cacgtctgct cggctcgaac attcattcac aactgatgct
     5941 ctcagccacc ccgcgccctt ttataccgct gcgctcgatc cccgggcgag ctcggccggc
     6001 cgtttatcac cactttgtac aagaaagctg aacgagaaac gtaaaatgat ataaatatca
     6061 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
     6121 tccagtcact atggtcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt
     6181 ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc
     6241 acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag
     6301 ctttcatccc cgatatgcac caccgggtaa agttcacggg agactttatc tgacagcaga
     6361 cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt
     6421 acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt
     6481 tcaccagccc ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc gggcgacctc
     6541 agccatccct tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt
     6601 ctgcatggtt gtgcttacca gaccggagat attgacatca tatatgcctt gagcaactga
     6661 tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcatacct ctttttgaca
     6721 tacttcgggt atacatatca gtatatattc ttataccgca aaaatcagcg cgcaaatacg
     6781 catactgtta tctggctttt agtaagccgg atccacgcgt ttacgccccg ccctgccact
     6841 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     6901 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     6961 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     7021 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     7081 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     7141 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     7201 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     7261 ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     7321 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     7381 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     7441 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     7501 aaaatctcga cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg
     7561 taaagcctgg ggtgcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta
     7621 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
     7681 cgtttctcgt tcagcttttt tgtacaaact tgtgataaac gaattcttga agacgaaagg
     7741 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagactc
     7801 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7861 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7921 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7981 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8041 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8101 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8161 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8221 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8281 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8341 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8401 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8461 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8521 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8581 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8641 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8701 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8761 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8821 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8881 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9241 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9301 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9361 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9421 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9481 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9541 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9601 tgctcacatg ttaccgtcga cgatgtaggt cacggtctcg aagccgcggt gcgggtgcca
     9661 gggcgtgccc ttgggctccc cgggcgcgta ctccacctca cccatctggt ccatcatgat
     9721 gaacgggtcg aggtggcggt agttgatccc ggcgaacgcg cggcgcaccg ggaagccctc
     9781 gccctcgaaa ccgctgggcg cggtggtcac ggtgagcacg ggacgtgcga cggcgtcgg
//
