LOCUS       sequence-320104-        9690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This insert, when expressed in the same cell with other split Cre 
            component, forms Cre recombinase through protein trans-splicing 
            using split intein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Luan H, Kuzin A, Odenwald WF, White BH
  TITLE     Cre-assisted fine-mapping of neural circuits using orthogonal split 
            inteins.
  JOURNAL   Elife. 2020 Apr 14;9. pii: 53041. doi: 10.7554/eLife.53041.
  PUBMED    32286225
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-04-14"; volume: "9. pii: 53041. doi"; pages: " 
            10.7554/eLife.53041"
COMMENT     Sequence Label: HJP-179-IVS-Syn21-NrdJ-1C-CreC-WPREw
FEATURES             Location/Qualifiers
     source          1..9690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..125
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(150..155,156..173,174..180)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             234..893
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(300..319)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1235..1540
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1419..1438
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1581..1705)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          1744..1810
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     misc_feature    2255..2843
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2308..2328)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2726..2737)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3104..3120)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3104..3120)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(4131..4745,4816..4947,5151..5466,5528..6182,
                     6257..6530,6932..7003))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     7455..7473
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    7698..7767
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(7854..8442)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7934..7953)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8613..9404,9405..9473))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9236..9255
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9474..9578)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9645..9663
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
       61 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      121 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
      181 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
      241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      361 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      421 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      481 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      541 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      601 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      661 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
      721 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
      781 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
      841 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
      901 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
      961 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     1021 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1081 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1141 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     1201 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     1261 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     1321 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     1381 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     1441 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     1501 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     1561 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     1621 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     1681 gttcagcttt cttgtacaaa gtggtgataa acggccggcc agatctggta ccgctagaaa
     1741 aaggtaggtt caaccactga tgcctaggca caccgaaacg actaacccta attcttatcc
     1801 tttacttcag ggctagcaac ttaaaaaaaa aaatcaaaat ggaggccaag acctacatcg
     1861 gcaagctgaa gagccgcaag atcgtgagca acgaggatac ctacgatatc cagaccagca
     1921 cccacaactt cttcgccaac gatatcctgg tgcacaactc ggcaacaagt cagttgagta
     1981 cgcgcgcgtt ggagggaatc ttcgaggcca ctcaccgatt gatttacggt gcaaaagatg
     2041 actccggcca acgttacttg gcctggtccg gtcacagcgc acgtgtgggt gccgcccgtg
     2101 acatggcgag ggccggtgtt tccatcccag aaatcatgca agcgggcgga tggaccaacg
     2161 tgaatattgt aatgaactac attcgtaatc tggatagtga aacaggcgca atggtgcgcc
     2221 tgctggagga cggagattaa accggtgttt aaacaatcaa cctctggatt acaaaatttg
     2281 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     2341 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     2401 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     2461 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     2521 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     2581 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     2641 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     2701 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     2761 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     2821 ctccctttgg gccgcctccc cgcctgaagc ttatcgatac cgtcgactaa agccaaatag
     2881 aaaattattc agttcctggc ttaagttttt aaaagtgata ttatttattt ggttgtaacc
     2941 aaccaaaaga atgtaaataa ctaatacata attatgttag ttttaagtta gcaacaaatt
     3001 gattttagct atattagcta cttggttaat aaatagaata tatttattta aagataattg
     3061 cgtttttatt gtcagggagt gagtttgctt aaaaactcgt ttagatccac tagttctaga
     3121 gcggccgctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
     3181 ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
     3241 actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
     3301 tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggaggcgcg
     3361 ccctagttcc agtgaaatcc aagcattttc taaattaaat gtattcttat tattatagtt
     3421 gttatttttg atatatataa acaacactat tatgcccacc atttttttga gatgcatcta
     3481 cacaaggaac aaacactgga tgtcactttc agttcaaatt gtaacgctaa tcactccgaa
     3541 caggtcacaa aaaattacct taaaaagtca taatattaaa ttagaataaa tatagctgtg
     3601 agggaaatat atacaaatat attggagcaa ataaattgta catacaaata tttattacta
     3661 atttctattg agacgaaatg aaccactcgg aaccatttga gcgaaccgaa tcgcgcggaa
     3721 ctaacgacag tcgctccaag gtcgtcgaac aaaaggtgaa tgtgttgcgg agagcgggtg
     3781 ggagacagcg aaagagcaac tacgaaacgt ggtgtggtgg aggtgaatta tgaagagggc
     3841 gcgcgatttg aaaagtatgt atataaaaaa tatatcccgg tgttttatgt agcgataaac
     3901 gagtttttga tgtaaggtat gcaggtgtgt aagtcttttg gttagaagac aaatccaaag
     3961 tctacttgtg gggatgttcg aaggggaaat acttgtattc tataggtcat atcttgtttt
     4021 tattggcaca aatataatta cattagcttt ttgagggggc aataaacagt aaacacgatg
     4081 gtaataatgg taaaaaaaaa aaacaagcag ttatttcgga tatatgtcgg ctactccttg
     4141 cgtcgggccc gaagtcttag agccagatat gcgagcaccc ggaagctcac gatgagaatg
     4201 gccagaccca cgtagtccag cggcagatcg gcggcggaga agttaagcgt ctccaggatg
     4261 accttgcccg aactggggca cgtggtgttc gacgatgtgc agctaatttc gcccggctcc
     4321 acgtccgccc attggttaat cagcagaccc tcgttggcgt aacggaacca tgagaggtac
     4381 gacaaccatt tgaggtatac tggcaccgag cccgagttca agaagaagcc gccaaagagc
     4441 aggaatggta tgataaccgg cggacccaca gacagcgcca tcgaggtcga ggagctggcg
     4501 caggatatta gatatccgaa ggacgttgac acattggcca ccagagtgac cagcgccagg
     4561 cagttgaaga agtgcagcac tccggcccgc agtccgatca tcggataggc aatcgccgtg
     4621 aagaccagtg gcactgtgag aaaaagcggc aattcggcaa tcgttttgcc cagaaagtat
     4681 gtgtcacagc gataaagtcg acttcgggcc tccctcataa aaactggcag ctctgaggtg
     4741 aacacctaaa tcgaatcgat tcattagaaa gttagtaaat tattgaaatg caaatgtatt
     4801 ctaaacatga cttacattta tcgtggcaaa gacgttttga aaggtcatgt tggtcaggaa
     4861 gaggaagatg gctccgttga tattcatcac acccacttgc gtgagttgtt ggcccaaaaa
     4921 gatgaggcca atcaagatgg caaccatctg caaattaaaa tgttactcgc atctcattaa
     4981 tattcgcgag ttaaatgaaa tttatttatc ttctgcaaaa ctataaacta tacatctcat
     5041 tgaaaaaaac taagaagggt gtggaatcag gcaattctat ctaaaatcta gcgaatttgt
     5101 ttccaagaat tgtaagcgtt atatcatttg tttccactgg aaccactcac cgttgtctga
     5161 ataagtcgca cttttacgag gagtggttcc ttgagcaccg acagccagga tcgccacagg
     5221 accgcccgga actgcatgaa ccaggtggcc ttgtaggtgt acccattctc cggctgctcc
     5281 agtggcttct ccagattttt ggtggccaac aactgctcca tatcccgggc tactttgcta
     5341 atggcaaaat tgtcgcatat cttggcgatc cgatcacggg actcgatctc ccgtccgggc
     5401 acaacggcca acacctgtac gtaaaagtcc gccggattgt agttggtagg acactgggca
     5461 cccacgctgg ataggagttg agatgttatg taatactaga tacccttaat aaacacatcg
     5521 aactcactag gaaaagaagt cgacggcttc gctgggagtg cccaagaaag ctaccctgcc
     5581 ctcggccatc agaaggatct tgtcaaagag ctcaaacagc tcggaagacg gctgatgaat
     5641 ggtcaggatg acggtcttgc ccttctgcga cagcttcttc agcacctgga cgacgctgtg
     5701 ggcggtaaag gagtccagtc cggaggtggg ctcatcgcag atcagaagcg gcggatcggt
     5761 tagagcctcg gaggcgaatg ccagacgctt cctttctccg ccggacagac ctttcaccct
     5821 gccgggcaca ccgatgatcg tgtgctgaca tttgctgagc gaaagctcct ggatcacctg
     5881 atccacgcgg gccactcgct gccgataggt cagatgtcgt ggcatccgca ccatggcttg
     5941 gaaaatcagg tgttccctgg ccgttaggga gccgataaag aggtcatcct gctggacata
     6001 ggcgcacctg gcctgcatct ccttggcgtc cacaggttgg ccattgagca gtcgcatccc
     6061 ggatggcgat acttggatgc cctgcggcga tcgaaaggca agggcattca gcagggtcgt
     6121 ctttccggca ccggaactgc ccatcacggc caaaagttcg cccggatagg ccacgccgca
     6181 aactgagttt caaattggta attggaccct ttattaagat ttcacacaga tcagccgact
     6241 gcgaatagaa actcaccgtt cttgagcaaa tgtttcctgg gcgccggtat gtgtcgctcg
     6301 ttgcagaata gtccgcgtgt ccggttgacc agctgccgcc atccggagcc cggctgattg
     6361 accgccccaa agatgtccat attgtgccag gcataggtga ggttctcggc tagttggccg
     6421 ctccctgaac cggagtcctc cggcggactg ggtggccgga gcgtgccgta gtttttggcc
     6481 tgcccgaagc cctggttaat gcagctctgc gaagccgctc cgctgtcacc ctgcaatgat
     6541 aggggatctc aaatatcaac tacaagcgtt atgctcatct aaccccgaac aaaaagtacc
     6601 ccgaagtatc ctacgaagta ggtttatact tttatttatt ttttgtgcat ctaggatcag
     6661 cttaaaatat ctggttgtta tattttttgt aaaaaagaat atagtcgaaa atgaatgcct
     6721 ttagatgtct tgatcatgat atgatctcaa aaattgtctt atatagcgag aacagctacc
     6781 agaataatct gtttcgtgtc actatttgtt tgtgcaattg cggtttggga tttttgtggg
     6841 tcgcagttct cacgccgcag acaatttgat gttgcaatcg cagttcctat agatcaagtg
     6901 aacttaagat gtatgcacat gtactactca cattgttcag atgctcggca gatgggtgtt
     6961 tgctgcctcc gcgaattaat agctcctgat cctcttggcc cattgccggg atttttcaca
     7021 ctttcccctg cttacccacc caaaaccaat caccacccca atcactcaaa aaacaaacaa
     7081 aaataagaag cgagaggagt tttggcacag cactttgtgt ttaattgatg gcgtaaaccg
     7141 cttggagctt cgtcacgaaa ccgctgacaa aatgcaactg aaggcggaca ttgacgctac
     7201 gtaacgctac aaacggtggc gaaagagata gcggacgcag cggcgaaaga gacggcgata
     7261 tttctgtgga cagagaagga ggcaaacagc gctgactttg agtggaatgt cattttgagt
     7321 gagaggtaat cgaaagaacc tggtacatca aatacccttg gatcgaagta aatttaaaac
     7381 tgatcagata agttcaatga tatccagtgc agtaaaaaaa aaaaatgttt tttttatcta
     7441 ctttccgcaa aaatgggttt tattaactta catacatact aggcgcgccc atatgttcgg
     7501 cttgtcgaca tgcccgccgt gaccgtcgag aacccgctga cgctgccccg cgtatccgca
     7561 cccgccgacg ccgtcgcacg tcccgtgctc accgtgacca ccgcgcccag cggtttcgag
     7621 ggcgagggct tcccggtgcg ccgcgcgttc gccgggatca actaccgcca cctcgacccg
     7681 ttcatcatga tggaccagat gggtgaggtg gagtacgcgc ccggggagcc caagggcacg
     7741 ccctggcacc cgcaccgcgg cttcgagacc gtgacctaca tcgtcgacgg taacatgtga
     7801 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7861 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7921 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7981 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8041 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8101 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8161 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8221 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8281 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8341 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8401 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8461 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8521 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8581 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8641 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8701 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8761 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8821 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8881 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8941 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9001 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9061 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9121 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9181 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9241 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9301 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9361 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9421 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9481 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9541 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9601 cctgagtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     9661 ggccctttcg tcttcaagaa ttcgtttatc
//
