LOCUS       sequence-320105-       10031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This insert, when expressed in the same cell with other split Cre 
            component, forms Cre recombinase through protein trans-splicing 
            using split intein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Luan H, Kuzin A, Odenwald WF, White BH
  TITLE     Cre-assisted fine-mapping of neural circuits using orthogonal split 
            inteins.
  JOURNAL   Elife. 2020 Apr 14;9. pii: 53041. doi: 10.7554/eLife.53041.
  PUBMED    32286225
REFERENCE   2  (bases 1 to 10031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-04-14"; volume: "9. pii: 53041. doi"; pages: " 
            10.7554/eLife.53041"
COMMENT     Sequence Label: HJP-194-IVS-Syn21-CreA-gp41-1N-WPREUw
FEATURES             Location/Qualifiers
     source          1..10031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(153..171)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        238..342
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(343..411,412..1203)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(561..580)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1374..1962
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1863..1882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2049..2118
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2343..2361)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2813..2884,3286..3559,3634..4288,4350..4665,4869..5000,
                     5071..5685)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     6696..6712
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6696..6712
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    6973..7561
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7079..7090
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7488..7508
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     intron          8198..8264
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     protein_bind    8452..8576
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(8617..8922)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8719..8738)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(9264..9923)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     9838..9857
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(9977..9983,9984..10001,10002..10007))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt tttgtacaaa
      121 cttgtgataa acgaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
      181 taatgtcatg ataataatgg tttcttagac tcaggtggca cttttcgggg aaatgtgcgc
      241 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      301 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
      361 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
      421 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
      481 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
      541 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
      601 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      661 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      721 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      781 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      841 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      901 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      961 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     1021 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     1081 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     1141 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     1201 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     1261 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     1321 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1381 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1441 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     1501 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     1561 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1621 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1681 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1741 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1801 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1861 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1921 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1981 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttaccgtc gacgatgtag
     2041 gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg
     2101 tactccacct cacccatctg gtccatcatg atgaacgggt cgaggtggcg gtagttgatc
     2161 ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga aaccgctggg cgcggtggtc
     2221 acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg atacgcgggg cagcgtcagc
     2281 gggttctcga cggtcacggc gggcatgtcg acaagccgaa catatgggcg cgcctagtat
     2341 gtatgtaagt taataaaacc catttttgcg gaaagtagat aaaaaaaaca tttttttttt
     2401 ttactgcact ggatatcatt gaacttatct gatcagtttt aaatttactt cgatccaagg
     2461 gtatttgatg taccaggttc tttcgattac ctctcactca aaatgacatt ccactcaaag
     2521 tcagcgctgt ttgcctcctt ctctgtccac agaaatatcg ccgtctcttt cgccgctgcg
     2581 tccgctatct ctttcgccac cgtttgtagc gttacgtagc gtcaatgtcc gccttcagtt
     2641 gcattttgtc agcggtttcg tgacgaagct ccaagcggtt tacgccatca attaaacaca
     2701 aagtgctgtg ccaaaactcc tctcgcttct tatttttgtt tgttttttga gtgattgggg
     2761 tggtgattgg ttttgggtgg gtaagcaggg gaaagtgtga aaaatcccgg caatgggcca
     2821 agaggatcag gagctattaa ttcgcggagg cagcaaacac ccatctgccg agcatctgaa
     2881 caatgtgagt agtacatgtg catacatctt aagttcactt gatctatagg aactgcgatt
     2941 gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac aaaaatccca aaccgcaatt
     3001 gcacaaacaa atagtgacac gaaacagatt attctggtag ctgttctcgc tatataagac
     3061 aatttttgag atcatatcat gatcaagaca tctaaaggca ttcattttcg actatattct
     3121 tttttacaaa aaatataaca accagatatt ttaagctgat cctagatgca caaaaaataa
     3181 ataaaagtat aaacctactt cgtaggatac ttcggggtac tttttgttcg gggttagatg
     3241 agcataacgc ttgtagttga tatttgagat cccctatcat tgcagggtga cagcggagcg
     3301 gcttcgcaga gctgcattaa ccagggcttc gggcaggcca aaaactacgg cacgctccgg
     3361 ccacccagtc cgccggagga ctccggttca gggagcggcc aactagccga gaacctcacc
     3421 tatgcctggc acaatatgga catctttggg gcggtcaatc agccgggctc cggatggcgg
     3481 cagctggtca accggacacg cggactattc tgcaacgagc gacacatacc ggcgcccagg
     3541 aaacatttgc tcaagaacgg tgagtttcta ttcgcagtcg gctgatctgt gtgaaatctt
     3601 aataaagggt ccaattacca atttgaaact cagtttgcgg cgtggcctat ccgggcgaac
     3661 ttttggccgt gatgggcagt tccggtgccg gaaagacgac cctgctgaat gcccttgcct
     3721 ttcgatcgcc gcagggcatc caagtatcgc catccgggat gcgactgctc aatggccaac
     3781 ctgtggacgc caaggagatg caggccaggt gcgcctatgt ccagcaggat gacctcttta
     3841 tcggctccct aacggccagg gaacacctga ttttccaagc catggtgcgg atgccacgac
     3901 atctgaccta tcggcagcga gtggcccgcg tggatcaggt gatccaggag ctttcgctca
     3961 gcaaatgtca gcacacgatc atcggtgtgc ccggcagggt gaaaggtctg tccggcggag
     4021 aaaggaagcg tctggcattc gcctccgagg ctctaaccga tccgccgctt ctgatctgcg
     4081 atgagcccac ctccggactg gactccttta ccgcccacag cgtcgtccag gtgctgaaga
     4141 agctgtcgca gaagggcaag accgtcatcc tgaccattca tcagccgtct tccgagctgt
     4201 ttgagctctt tgacaagatc cttctgatgg ccgagggcag ggtagctttc ttgggcactc
     4261 ccagcgaagc cgtcgacttc ttttcctagt gagttcgatg tgtttattaa gggtatctag
     4321 tattacataa catctcaact cctatccagc gtgggtgccc agtgtcctac caactacaat
     4381 ccggcggact tttacgtaca ggtgttggcc gttgtgcccg gacgggagat cgagtcccgt
     4441 gatcggatcg ccaagatatg cgacaatttt gccattagca aagtagcccg ggatatggag
     4501 cagttgttgg ccaccaaaaa tctggagaag ccactggagc agccggagaa tgggtacacc
     4561 tacaaggcca cctggttcat gcagttccgg gcggtcctgt ggcgatcctg gctgtcggtg
     4621 ctcaaggaac cactcctcgt aaaagtgcga cttattcaga caacggtgag tggttccagt
     4681 ggaaacaaat gatataacgc ttacaattct tggaaacaaa ttcgctagat tttagataga
     4741 attgcctgat tccacaccct tcttagtttt tttcaatgag atgtatagtt tatagttttg
     4801 cagaagataa ataaatttca tttaactcgc gaatattaat gagatgcgag taacatttta
     4861 atttgcagat ggttgccatc ttgattggcc tcatcttttt gggccaacaa ctcacgcaag
     4921 tgggtgtgat gaatatcaac ggagccatct tcctcttcct gaccaacatg acctttcaaa
     4981 acgtctttgc cacgataaat gtaagtcatg tttagaatac atttgcattt caataattta
     5041 ctaactttct aatgaatcga ttcgatttag gtgttcacct cagagctgcc agtttttatg
     5101 agggaggccc gaagtcgact ttatcgctgt gacacatact ttctgggcaa aacgattgcc
     5161 gaattgccgc tttttctcac agtgccactg gtcttcacgg cgattgccta tccgatgatc
     5221 ggactgcggg ccggagtgct gcacttcttc aactgcctgg cgctggtcac tctggtggcc
     5281 aatgtgtcaa cgtccttcgg atatctaata tcctgcgcca gctcctcgac ctcgatggcg
     5341 ctgtctgtgg gtccgccggt tatcatacca ttcctgctct ttggcggctt cttcttgaac
     5401 tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt ccgttacgcc
     5461 aacgagggtc tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat tagctgcaca
     5521 tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc tggagacgct taacttctcc
     5581 gccgccgatc tgccgctgga ctacgtgggt ctggccattc tcatcgtgag cttccgggtg
     5641 ctcgcatatc tggctctaag acttcgggcc cgacgcaagg agtagccgac atatatccga
     5701 aataactgct tgtttttttt ttttaccatt attaccatcg tgtttactgt ttattgcccc
     5761 ctcaaaaagc taatgtaatt atatttgtgc caataaaaac aagatatgac ctatagaata
     5821 caagtatttc cccttcgaac atccccacaa gtagactttg gatttgtctt ctaaccaaaa
     5881 gacttacaca cctgcatacc ttacatcaaa aactcgttta tcgctacata aaacaccggg
     5941 atatattttt tatatacata cttttcaaat cgcgcgccct cttcataatt cacctccacc
     6001 acaccacgtt tcgtagttgc tctttcgctg tctcccaccc gctctccgca acacattcac
     6061 cttttgttcg acgaccttgg agcgactgtc gttagttccg cgcgattcgg ttcgctcaaa
     6121 tggttccgag tggttcattt cgtctcaata gaaattagta ataaatattt gtatgtacaa
     6181 tttatttgct ccaatatatt tgtatatatt tccctcacag ctatatttat tctaatttaa
     6241 tattatgact ttttaaggta attttttgtg acctgttcgg agtgattagc gttacaattt
     6301 gaactgaaag tgacatccag tgtttgttcc ttgtgtagat gcatctcaaa aaaatggtgg
     6361 gcataatagt gttgtttata tatatcaaaa ataacaacta taataataag aatacattta
     6421 atttagaaaa tgcttggatt tcactggaac tagggcgcgc ctccggaaca taatggtgca
     6481 gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt
     6541 tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg
     6601 tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag
     6661 gagcacgatc atgcgcaccc gtggccagcg gccgctctag aactagtgga tctaaacgag
     6721 tttttaagca aactcactcc ctgacaataa aaacgcaatt atctttaaat aaatatattc
     6781 tatttattaa ccaagtagct aatatagcta aaatcaattt gttgctaact taaaactaac
     6841 ataattatgt attagttatt tacattcttt tggttggtta caaccaaata aataatatca
     6901 cttttaaaaa cttaagccag gaactgaata attttctatt tggctttagt cgacggtatc
     6961 gataagcttc aggcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc
     7021 gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat
     7081 tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg
     7141 cgagcagcca aggaaaggac gatgatttcc ccgacaacac cacggaattg tcagtgccca
     7201 acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa
     7261 tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac
     7321 cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc
     7381 acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca
     7441 tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag
     7501 gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat
     7561 tgtttaaaca ccggtttact ccttcacgta caggcacatg ccctccttca ggccgccgct
     7621 gatgttcatc tcgccggtct gggtggggaa caggtgctcc tcgctgcaga tgatctcctt
     7681 gccatcctcc agggtgatct tgtagctctt cttcttgctc ttggggaaca cgttcagcac
     7741 ctcgttgtag ccggtgttgc tcagcaccag atcgcccacc tggatgttgc tgatctcctt
     7801 catgccctgg ggggtctgca cctgggtctt cagatccagg caatcggatg gccggggcag
     7861 tccgctgcgc ctgtggagca tgttcagctg acccaaatgt tgctgtattg ttttgacggc
     7921 cagtccgcga gcctgcaggt acagcagata atcgcggacg tcctcgggct ccgcgggaaa
     7981 ccactttcgg ttgttcagct tgcaccaagc ggcccaggac ctgcacaccg agagcagcat
     8041 cttccaggtg tgctcgctaa aggcctgcct gtcacggaac atgtccatca gattctttcg
     8101 gacctcgtcg gacgtggcgt ccacaggcag ggccggcaaa ttttggtgta ccgtgagcaa
     8161 gttactcatt ttgatttttt tttttaagtt gctagccctg aagtaaagga taagaattag
     8221 ggttagtcgt ttcggtgtgc ctaggcatca gtggttgaac ctaccttttt ctagcggtac
     8281 cgtttggtat gcgtcttgtg attcaaagtt ggcttattca aaggatatta acgaaggcag
     8341 cggcacgtct gctcggctcg aacattcatt cacaactgat gctctcagcc accccgcgcc
     8401 cttttatacc gctgcgctcg atccccgggc gagctcggcc ggccgtttat caccactttg
     8461 tacaagaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt
     8521 ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc actatggtcg
     8581 acctgcagac tggctgtgta taagggagcc tgacatttat attccccaga acatcaggtt
     8641 aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac
     8701 ggagaccggc acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg
     8761 caccaccggg taaagttcac gggagacttt atctgacagc agacgtgcac tggccagggg
     8821 gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca caaacagacg
     8881 ataacggctc tctcttttat aggtgtaaac cttaaactgc atttcaccag cccctgttct
     8941 cgtcagcaaa agagccgttc atttcaataa accgggcgac ctcagccatc ccttcctgat
     9001 tttccgcttt ccagcgttcg gcacgcagac gacgggcttc attctgcatg gttgtgctta
     9061 ccagaccgga gatattgaca tcatatatgc cttgagcaac tgatagctgt cgctgtcaac
     9121 tgtcactgta atacgctgct tcatagcata cctctttttg acatacttcg ggtatacata
     9181 tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat acgcatactg ttatctggct
     9241 tttagtaagc cggatccacg cgtttacgcc ccgccctgcc actcatcgca gtactgttgt
     9301 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     9361 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     9421 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     9481 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     9541 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     9601 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     9661 acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca
     9721 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     9781 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     9841 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     9901 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgacggatcc
     9961 taactcaaaa tccacacatt atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    10021 aatgcggccg c
//
