LOCUS       sequence-320107-       10505 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This insert, when expressed in the same cell with other split Cre 
            component, forms Cre recombinase through protein trans-splicing 
            using split intein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10505)
  AUTHORS   Luan H, Kuzin A, Odenwald WF, White BH
  TITLE     Cre-assisted fine-mapping of neural circuits using orthogonal split 
            inteins.
  JOURNAL   Elife. 2020 Apr 14;9. pii: 53041. doi: 10.7554/eLife.53041.
  PUBMED    32286225
REFERENCE   2  (bases 1 to 10505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-04-14"; volume: "9. pii: 53041. doi"; pages: " 
            10.7554/eLife.53041"
COMMENT     Sequence Label: HJP-208-IVS-Syn21-CreAB-NrdJ-1N-WPREUw
FEATURES             Location/Qualifiers
     source          1..10505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     67..85
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    113..237
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(262..267,268..285,286..292)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             346..1005
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(412..431)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1347..1652
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1531..1550
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1693..1817)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          2005..2071
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     misc_feature    3182..3770
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3235..3255)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3653..3664)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4031..4047)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4031..4047)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(5058..5672,5743..5874,6078..6393,6455..7109,
                     7184..7457,7859..7930))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     8382..8400
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    8625..8694
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(8781..9369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8861..8880)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9540..10331,10332..10400))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10163..10182
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10401..10505)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgagtc taagaaacca ttattatcat gacattaacc
       61 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattcgttta tcacaagttt
      121 gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat
      181 tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt cactatggcg
      241 gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt
      301 ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
      361 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
      421 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
      481 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
      541 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
      601 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
      661 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
      721 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
      781 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
      841 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
      901 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
      961 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc
     1021 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
     1081 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
     1141 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
     1201 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
     1261 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
     1321 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
     1381 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
     1441 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
     1501 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
     1561 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
     1621 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
     1681 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
     1741 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
     1801 ttcttgtaca aagtggtgat aaacggccgg ccgagctcgc ccggggatcg agcgcagcgg
     1861 tataaaaggg cgcggggtgg ctgagagcat cagttgtgaa tgaatgttcg agccgagcag
     1921 acgtgccgct gccttcgtta atatcctttg aataagccaa ctttgaatca caagacgcat
     1981 accaaacggt accgctagaa aaaggtaggt tcaaccactg atgcctaggc acaccgaaac
     2041 gactaaccct aattcttatc ctttacttca gggctagcaa cttaaaaaaa aaaatcaaaa
     2101 tgagtaactt gctcacggta caccaaaatt tgccggccct gcctgtggac gccacgtccg
     2161 acgaggtccg aaagaatctg atggacatgt tccgtgacag gcaggccttt agcgagcaca
     2221 cctggaagat gctgctctcg gtgtgcaggt cctgggccgc ttggtgcaag ctgaacaacc
     2281 gaaagtggtt tcccgcggag cccgaggacg tccgcgatta tctgctgtac ctgcaggctc
     2341 gcggactggc cgtcaaaaca atacagcaac atttgggtca gctgaacatg ctccacaggc
     2401 gcagcggact gccccggcca tccgatagca atgcagtgag cctggtaatg cgtcgaatac
     2461 gcaaggaaaa cgtggacgcc ggcgagaggg ccaaacaggc gttggctttc gaacgcaccg
     2521 atttcgatca ggtgcggtcg ttgatggaga acagcgatcg atgccaggac atccggaact
     2581 tggcgttttt gggcattgcc tataacaccc tccttcgcat cgccgagata gcgcgtatcc
     2641 gagttaaaga catttcccgt acagacggag gccgcatgct tattcacatc ggacgcacga
     2701 aaaccctggt cagcactgct ggcgtggaga aagcactttc cctgggtgtg acgaaattgg
     2761 tcgagcgatg gatcagtgtg agtggcgtcg ccgatgaccc caacaattat ctgttctgca
     2821 gggtgcgcaa aaatggagtg gcagccccct gcctggtggg cagcagcgag atcatcaccc
     2881 gcaactacgg caagaccacc atcaaggagg tggtggagat cttcgataac gataagaaca
     2941 tccaggtgct ggccttcaac acccacaccg ataacatcga gtgggccccc atcaaggccg
     3001 cccagctgac ccgccccaac gccgagctgg tggagctgga gatcgatacc ctgcacggcg
     3061 tgaagaccat ccgctgcacc cccgatcacc ccgtgtacac caagaaccgc ggctacgtgc
     3121 gcgccgatga gctgaccgat gatgatgagc tggtggtggc catctaaacc ggtgtttaaa
     3181 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     3241 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     3301 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     3361 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     3421 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     3481 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     3541 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     3601 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     3661 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     3721 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgaagctta
     3781 tcgataccgt cgactaaagc caaatagaaa attattcagt tcctggctta agtttttaaa
     3841 agtgatatta tttatttggt tgtaaccaac caaaagaatg taaataacta atacataatt
     3901 atgttagttt taagttagca acaaattgat tttagctata ttagctactt ggttaataaa
     3961 tagaatatat ttatttaaag ataattgcgt ttttattgtc agggagtgag tttgcttaaa
     4021 aactcgttta gatccactag ttctagagcg gccgctggcc acgggtgcgc atgatcgtgc
     4081 tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat
     4141 caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa
     4201 caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc
     4261 cctgcaccat tatgttccgg aggcgcgccc tagttccagt gaaatccaag cattttctaa
     4321 attaaatgta ttcttattat tatagttgtt atttttgata tatataaaca acactattat
     4381 gcccaccatt tttttgagat gcatctacac aaggaacaaa cactggatgt cactttcagt
     4441 tcaaattgta acgctaatca ctccgaacag gtcacaaaaa attaccttaa aaagtcataa
     4501 tattaaatta gaataaatat agctgtgagg gaaatatata caaatatatt ggagcaaata
     4561 aattgtacat acaaatattt attactaatt tctattgaga cgaaatgaac cactcggaac
     4621 catttgagcg aaccgaatcg cgcggaacta acgacagtcg ctccaaggtc gtcgaacaaa
     4681 aggtgaatgt gttgcggaga gcgggtggga gacagcgaaa gagcaactac gaaacgtggt
     4741 gtggtggagg tgaattatga agagggcgcg cgatttgaaa agtatgtata taaaaaatat
     4801 atcccggtgt tttatgtagc gataaacgag tttttgatgt aaggtatgca ggtgtgtaag
     4861 tcttttggtt agaagacaaa tccaaagtct acttgtgggg atgttcgaag gggaaatact
     4921 tgtattctat aggtcatatc ttgtttttat tggcacaaat ataattacat tagctttttg
     4981 agggggcaat aaacagtaaa cacgatggta ataatggtaa aaaaaaaaaa caagcagtta
     5041 tttcggatat atgtcggcta ctccttgcgt cgggcccgaa gtcttagagc cagatatgcg
     5101 agcacccgga agctcacgat gagaatggcc agacccacgt agtccagcgg cagatcggcg
     5161 gcggagaagt taagcgtctc caggatgacc ttgcccgaac tggggcacgt ggtgttcgac
     5221 gatgtgcagc taatttcgcc cggctccacg tccgcccatt ggttaatcag cagaccctcg
     5281 ttggcgtaac ggaaccatga gaggtacgac aaccatttga ggtatactgg caccgagccc
     5341 gagttcaaga agaagccgcc aaagagcagg aatggtatga taaccggcgg acccacagac
     5401 agcgccatcg aggtcgagga gctggcgcag gatattagat atccgaagga cgttgacaca
     5461 ttggccacca gagtgaccag cgccaggcag ttgaagaagt gcagcactcc ggcccgcagt
     5521 ccgatcatcg gataggcaat cgccgtgaag accagtggca ctgtgagaaa aagcggcaat
     5581 tcggcaatcg ttttgcccag aaagtatgtg tcacagcgat aaagtcgact tcgggcctcc
     5641 ctcataaaaa ctggcagctc tgaggtgaac acctaaatcg aatcgattca ttagaaagtt
     5701 agtaaattat tgaaatgcaa atgtattcta aacatgactt acatttatcg tggcaaagac
     5761 gttttgaaag gtcatgttgg tcaggaagag gaagatggct ccgttgatat tcatcacacc
     5821 cacttgcgtg agttgttggc ccaaaaagat gaggccaatc aagatggcaa ccatctgcaa
     5881 attaaaatgt tactcgcatc tcattaatat tcgcgagtta aatgaaattt atttatcttc
     5941 tgcaaaacta taaactatac atctcattga aaaaaactaa gaagggtgtg gaatcaggca
     6001 attctatcta aaatctagcg aatttgtttc caagaattgt aagcgttata tcatttgttt
     6061 ccactggaac cactcaccgt tgtctgaata agtcgcactt ttacgaggag tggttccttg
     6121 agcaccgaca gccaggatcg ccacaggacc gcccggaact gcatgaacca ggtggccttg
     6181 taggtgtacc cattctccgg ctgctccagt ggcttctcca gatttttggt ggccaacaac
     6241 tgctccatat cccgggctac tttgctaatg gcaaaattgt cgcatatctt ggcgatccga
     6301 tcacgggact cgatctcccg tccgggcaca acggccaaca cctgtacgta aaagtccgcc
     6361 ggattgtagt tggtaggaca ctgggcaccc acgctggata ggagttgaga tgttatgtaa
     6421 tactagatac ccttaataaa cacatcgaac tcactaggaa aagaagtcga cggcttcgct
     6481 gggagtgccc aagaaagcta ccctgccctc ggccatcaga aggatcttgt caaagagctc
     6541 aaacagctcg gaagacggct gatgaatggt caggatgacg gtcttgccct tctgcgacag
     6601 cttcttcagc acctggacga cgctgtgggc ggtaaaggag tccagtccgg aggtgggctc
     6661 atcgcagatc agaagcggcg gatcggttag agcctcggag gcgaatgcca gacgcttcct
     6721 ttctccgccg gacagacctt tcaccctgcc gggcacaccg atgatcgtgt gctgacattt
     6781 gctgagcgaa agctcctgga tcacctgatc cacgcgggcc actcgctgcc gataggtcag
     6841 atgtcgtggc atccgcacca tggcttggaa aatcaggtgt tccctggccg ttagggagcc
     6901 gataaagagg tcatcctgct ggacataggc gcacctggcc tgcatctcct tggcgtccac
     6961 aggttggcca ttgagcagtc gcatcccgga tggcgatact tggatgccct gcggcgatcg
     7021 aaaggcaagg gcattcagca gggtcgtctt tccggcaccg gaactgccca tcacggccaa
     7081 aagttcgccc ggataggcca cgccgcaaac tgagtttcaa attggtaatt ggacccttta
     7141 ttaagatttc acacagatca gccgactgcg aatagaaact caccgttctt gagcaaatgt
     7201 ttcctgggcg ccggtatgtg tcgctcgttg cagaatagtc cgcgtgtccg gttgaccagc
     7261 tgccgccatc cggagcccgg ctgattgacc gccccaaaga tgtccatatt gtgccaggca
     7321 taggtgaggt tctcggctag ttggccgctc cctgaaccgg agtcctccgg cggactgggt
     7381 ggccggagcg tgccgtagtt tttggcctgc ccgaagccct ggttaatgca gctctgcgaa
     7441 gccgctccgc tgtcaccctg caatgatagg ggatctcaaa tatcaactac aagcgttatg
     7501 ctcatctaac cccgaacaaa aagtaccccg aagtatccta cgaagtaggt ttatactttt
     7561 atttattttt tgtgcatcta ggatcagctt aaaatatctg gttgttatat tttttgtaaa
     7621 aaagaatata gtcgaaaatg aatgccttta gatgtcttga tcatgatatg atctcaaaaa
     7681 ttgtcttata tagcgagaac agctaccaga ataatctgtt tcgtgtcact atttgtttgt
     7741 gcaattgcgg tttgggattt ttgtgggtcg cagttctcac gccgcagaca atttgatgtt
     7801 gcaatcgcag ttcctataga tcaagtgaac ttaagatgta tgcacatgta ctactcacat
     7861 tgttcagatg ctcggcagat gggtgtttgc tgcctccgcg aattaatagc tcctgatcct
     7921 cttggcccat tgccgggatt tttcacactt tcccctgctt acccacccaa aaccaatcac
     7981 caccccaatc actcaaaaaa caaacaaaaa taagaagcga gaggagtttt ggcacagcac
     8041 tttgtgttta attgatggcg taaaccgctt ggagcttcgt cacgaaaccg ctgacaaaat
     8101 gcaactgaag gcggacattg acgctacgta acgctacaaa cggtggcgaa agagatagcg
     8161 gacgcagcgg cgaaagagac ggcgatattt ctgtggacag agaaggaggc aaacagcgct
     8221 gactttgagt ggaatgtcat tttgagtgag aggtaatcga aagaacctgg tacatcaaat
     8281 acccttggat cgaagtaaat ttaaaactga tcagataagt tcaatgatat ccagtgcagt
     8341 aaaaaaaaaa aatgtttttt ttatctactt tccgcaaaaa tgggttttat taacttacat
     8401 acatactagg cgcgcccata tgttcggctt gtcgacatgc ccgccgtgac cgtcgagaac
     8461 ccgctgacgc tgccccgcgt atccgcaccc gccgacgccg tcgcacgtcc cgtgctcacc
     8521 gtgaccaccg cgcccagcgg tttcgagggc gagggcttcc cggtgcgccg cgcgttcgcc
     8581 gggatcaact accgccacct cgacccgttc atcatgatgg accagatggg tgaggtggag
     8641 tacgcgcccg gggagcccaa gggcacgccc tggcacccgc accgcggctt cgagaccgtg
     8701 acctacatcg tcgacggtaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8761 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8821 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8881 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8941 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9001 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9061 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9121 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9181 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9241 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9301 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9361 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9421 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9481 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9541 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9601 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9661 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9721 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9781 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9841 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9901 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9961 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10021 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10081 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10141 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10201 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10261 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10321 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10381 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10441 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10501 ccgcg
//
