LOCUS       sequence-320115-       12044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible expression of Atg4B(C74A) fused with 
            mStrawberry to inhibit autophagy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12044)
  AUTHORS   Yamamoto K, Venida A, Yano J, Biancur DE, Kakiuchi M, Gupta S, Sohn 
            ASW, Mukhopadhyay S, Lin EY, Parker SJ, Banh RS, Paulo JA, Wen KW, 
            Debnath J, Kim GE, Mancias JD, Fearon DT, Perera RM, Kimmelman AC
  TITLE     Autophagy promotes immune evasion of pancreatic cancer by degrading 
            MHC-I.
  JOURNAL   Nature. 2020 May;581(7806):100-105. doi: 10.1038/s41586-020-2229-5. 
            Epub 2020 Apr 22.
  PUBMED    32376951
REFERENCE   2  (bases 1 to 12044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2229-5"; journalName: "Nature"; date: "2020-05";
            volume: "581"; issue: "7806"; pages: "100-105"
COMMENT     Sequence Label: pINDUCER20_mStrawberry_Atg4BC74A
FEATURES             Location/Qualifiers
     source          1..12044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2731..3438
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /label=mStrawberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILTPNFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVELYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2876..2894)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3144..3163
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(4631..4655)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4708..4734
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4820..6031
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5868..5887
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6090..6707,6708..6713,6714..6749,6750..6752,6753..6788,
                     6789..6791,6792..6827,6828..6836)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6874..7447
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7041..7058)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7268..7287
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7463..8257
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7463..8257
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7517..7536)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8127..8146
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    8434..9022
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8487..8507)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8905..8916)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9097..9330
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9449..9467)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9535..9639
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9640..9708,9709..10500)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9858..9877)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10671..11259
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11160..11179
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11413..11430
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11670..11691
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11706..11711,11712..11729,11730..11736)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11744..11760
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11749..11771
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11768..11784
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11768..11784
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11834..11853
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggcttcacc atggtgagca agggcgagga gaataacatg
     2761 gccatcatca aggagttcat gcgcttcaag gtgcgcatgg agggctccgt gaacggccac
     2821 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     2881 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctaac ccccaacttc
     2941 acctacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     3001 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     3061 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     3121 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     3181 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gatgaggctg
     3241 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     3301 cccgtgcagc tgcccggcgc ctacatcgtc ggcatcaagt tggacatcac ctcccacaac
     3361 gaggactaca ccatcgtgga actgtacgaa cgcgccgagg gccgccactc caccggcggc
     3421 atggacgagc tgtacaagag atccatggac gcagccactt tgacatatga tactctccgg
     3481 tttgctgaat ttgaagattt ccctgagacc tcagagcctg tttggattct gggcagaaaa
     3541 tacagcattt tcacagagaa ggacgaaatc ttgtctgatg ttgcatccag actttggttt
     3601 acatacagga gaaactttcc agctattggg ggaactggcc ctacttcaga cacaggctgg
     3661 ggtgcaatgc ttcggtgtgg acagatgatc tttgcccagg ccctggtatg ccggcactta
     3721 ggtcgagatt ggaggtggac tcagcggaag aggcagcctg acagctactt taatgtcctc
     3781 aatgctttcc tcgacaggaa ggacagctac tattccatcc atcagatagc gcaaatggga
     3841 gttggcgaag gcaagtctat aggccagtgg tacgggccga acactgttgc ccaggtcctc
     3901 aagaaacttg ctgtattcga cacatggagt tccttggctg ttcacatagc aatggacaac
     3961 actgtggtga tggaggaaat cagaaggtta tgcagggcca accttccctg tgttggggcg
     4021 gctgcacttc ctactgattc agagaggcac tgtaatgggt tccctgctgg agctgaagtc
     4081 accaacaggc catcggcttg gagaccacta gtgcttctca tccctctccg cctgggactg
     4141 acagacatca atgaggccta tgtggagacg ctgaagcact gtttcatgat gccccagtcc
     4201 ctgggtgtta ttggagggaa gcccaacagt gcccactact ttattggcta tgttggggag
     4261 gagctcatct atctggaccc ccacactaca cagcctgcag tggagctgac tgacagctgc
     4321 ttcatccccg acgagagctt ccactgccag caccctccct ctaggatggg cattggagag
     4381 cttgacccgt ccattgctgt ggggtttttc tgtaagacgg aggaagactt taacgactgg
     4441 tgtcagcaag tcaaaaagct atcccagctt ggaggtgccc tgcccatgtt tgagctggtg
     4501 gaacagcagc cttcccatct ggcctgccag gatgtcctga acctgtccct agactcttct
     4561 gatgtagaga gactggagag gttttttgac tctgaggatg aagactttga aatcttatcc
     4621 ctctgataaa acccagcttt cttgtacaaa gtggttgata tccagcacag tggcggccgc
     4681 tcgagtctag agggcccgcg gttcgaatac ccatacgacg tcccagacta cgcttagtaa
     4741 tgattaatta aactagaaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     4801 agtaacccac ccaagatctg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc
     4861 ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc
     4921 gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat
     4981 cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca
     5041 gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg
     5101 tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttccg
     5161 tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg tcacttggtg
     5221 agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc ggtgggacgg
     5281 aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa
     5341 ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga atggaagacg
     5401 cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa
     5461 cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga gatgggctgg
     5521 ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt
     5581 ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag tgcacccgta cctttgggag
     5641 cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg cagggtgggg
     5701 ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct
     5761 agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg gataagtgag
     5821 gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga agctccggtt
     5881 ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg caccttttga
     5941 aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa
     6001 attctggccg tttttggctt ttttgttaga cgaagcttgg taccgagctc ggatctccac
     6061 cccgtaccgg tcctgcagtc gaattcacca tgtctagact ggacaagagc aaagtcataa
     6121 acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg
     6181 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc
     6241 tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg
     6301 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc
     6361 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg
     6421 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac
     6481 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc
     6541 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga
     6601 gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg
     6661 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg
     6721 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg
     6781 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaactaa
     6841 gtaaggatcc gcggccgcac tagaggaatt ccgcccctct cccccccccc cccctaacgt
     6901 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     6961 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     7021 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     7081 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     7141 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     7201 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     7261 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     7321 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     7381 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     7441 atgataagct taccggtcca ccatgattga acaagatgga ttgcacgcag gttctccggc
     7501 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     7561 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     7621 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     7681 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     7741 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     7801 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     7861 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     7921 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     7981 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     8041 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     8101 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     8161 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     8221 catcgccttc tatcgccttc ttgacgagtt cttctgatgt acaagtaaag cggccgcgac
     8281 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     8341 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt tagtccctcc
     8401 caattcgata tcaagcttat cgcgatagat cctaatcaac ctctggatta caaaatttgt
     8461 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8521 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     8581 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     8641 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     8701 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     8761 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     8821 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     8881 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     8941 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     9001 tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg
     9061 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     9121 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     9181 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     9241 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     9301 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     9361 atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa
     9421 gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     9481 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     9541 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9601 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     9661 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9721 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     9781 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     9841 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     9901 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     9961 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    10021 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    10081 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    10141 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    10201 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    10261 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    10321 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    10381 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    10441 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    10501 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    10561 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    10621 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    10681 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10741 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    10801 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    10861 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    10921 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    10981 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    11041 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    11101 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    11161 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    11221 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    11281 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    11341 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    11401 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    11461 cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt
    11521 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
    11581 gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac
    11641 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
    11701 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
    11761 tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca
    11821 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    11881 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    11941 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    12001 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
