LOCUS       sequence-320167-       16037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the BREX type I defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16037)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 16037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG002 (BREX type I)
FEATURES             Location/Qualifiers
     source          1..16037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..546)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      14483..14530
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(14750..15409)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     15324..15343
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(15410..15512)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(15904..15921)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg
      121 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc
      181 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
      241 acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      301 acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag
      361 ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc
      421 gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa
      481 ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga
      541 tctcaagaag atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc
      601 tcttcaaatg tagcacctga agtcagcccc atacgatata agttgtaatt ctcatgttag
      661 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc
      721 gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc aacagcacca
      781 cgttcatctt ccttttttaa ctgattttac agagacttta atacagttaa aattttattt
      841 cctgagctgt aatcgattaa gttgatgcat ttaatgggaa tgatataggg tcatttccag
      901 tctcacttat agaaatggct aaagcatgac tctcgccaaa accgtttatg tgttgtacat
      961 aacgcgatca tccctctcac aaattgcctt ttctcatggc atctcgcccg gtcccccatt
     1021 acaatcactt tttgtttttt gcgagctgca ttccagtctt cagagggttt ttcgatgatt
     1081 aaaaatgaca aggcatggat aggagacttg ctgggcggac cgctcatgag cagggaaagc
     1141 cgcgtcattg ccgaactgtt gctaaccgat cccgatgaac agacatggca agagcaaatt
     1201 gttggccaca acattttaca agcctcttct cctaacaccg caaaacgtta cgcggcaaca
     1261 atcaggcttc gcctgaacac gctggataaa agcgcgtgga cattgattgc cgaaggtagt
     1321 gaacgggaac gccaacaact tctgtttgtg gctctgatgc tacattcgcc ggtagttaag
     1381 gattttctgg ctgaagtggt gaacgatctg cgcaggcagt tcaaggaaaa gttgcctggc
     1441 aatagctgga acgaatttgt gaatagccag gttcgcctac atccggtact cgccagctac
     1501 tcagattcat ctattgcaaa aatgggaaac aatctggtga aggcgcttgc tgaagcgggt
     1561 tatgtggata cgccccgcag acgtaacctg caggcagttt accttttacc ggaaactcag
     1621 gcagtgttac agcgcctggg acaacaggac ttgatatcta ttctggaggg aaaacggtga
     1681 tagatcccgt tcttgaatat cgcctgtctc aaatccagag tcgcattaac gaagatcgct
     1741 tcctcaaaaa taacggctcc ggaaatgaaa ttggtttttg gatctttgat tatcccgcgc
     1801 agtgcgaact gcaggtacgg gagcatttga aatatctgct ccggcatctg gaaaaggacc
     1861 ataaatttgc ctgtctgaat gtcttccaaa tcatcatcga tatgctcaat gaacgcggcc
     1921 ttttcgagcg cgtctgccag caggaagtca aagtgggtac tgagacgctg aaaaagcagc
     1981 ttgctggtcc gttaaatcag aaaaagatcg ctgattttat agcgaaaaaa gtcgatctgg
     2041 ctgcccagga ttttgtcatt cttaccggca tgggcaacgc ctggccatta gtacgcggtc
     2101 atgaactgat gagtgccttg caggatgtca tggggttcac cccactgctg atgttttatc
     2161 ctggcaccta cagcgggtac aacctttccc cgctcacaga caccggttca caaaattatt
     2221 atcgcgcttt cagactggta ccagatacgg gacccgcagc aacattgaat cctcaatgaa
     2281 gagcataaca atgaatattg aacagatttt tgaaaaacct ctaaaacgaa atataaacgg
     2341 ggtagtcaaa gcagagcaaa ccgatgatgc cagcgcgtac atcgagttag atgaatatgt
     2401 catcacccgc gaactggaaa accatcttcg ccatttcttc gaatcctatg ttcctgccac
     2461 tggcccggaa cggatccgta tggaaaacaa gatcggcgta tgggtttcag gcttcttcgg
     2521 ttcaggtaaa tcgcacttta ttaagattct ttcttatctt ttatctaacc gcaaagttac
     2581 acataacggt acggaacgta atgcttactc cttctttgaa gataaaatca aagatgcatt
     2641 attccttgcc gatattaaca aagcggtgca ttacccgact gaagtcattc tgttcaatat
     2701 tgattcgcgt gccaacgtag atgacaaaga agatgccatt cttaaagtct tcctgaaagt
     2761 tttcaacgaa cgcattggat actgcgctga ttttccgcat attgcccatc ttgagcgcga
     2821 gctggataaa cgcggtcagt atgaaacctt taaagccgcg tttgccgata tcaatggctc
     2881 gcgctgggaa gacgagcgcg acgcttacta cttcatcagc gatgacatgg cacaagcatt
     2941 aagccaggcc acgcagcaga gtcttgaatc ctcccgccaa tgggtggaac aactcgacaa
     3001 aaacttcccg ctggatatca ataatttttg ccagtgggta aaagagtggc tggatgacaa
     3061 tggtaagaac atcctcttta tggtggatga agtcggtcag ttcattggca aaaatacgca
     3121 aatgatgctg aagctgcaga ctattactga aaaccttggg gtaatttgcg gtggccgcgc
     3181 atgggttatc gtgacttcgc aggccgatat caacgcggca atcggtggta tgagcagtcg
     3241 cgacggacag gacttctcca agatccaggg gcgcttctct acacgcctgc aactttccag
     3301 ctctaacaca tcagaagtta tccagaaacg tttgttggta aagactgacg aagcaaaagc
     3361 ggcactggca aaagtgtggc aagagaaagc cgatatcctg cgtaaccagc tggcttttga
     3421 cactacaaca actactgcac tacgtccttt taccagcgaa gaagagttcg ttgacaacta
     3481 cccgtttgtc ccgtggcact atcagattct gcaaaaagtg tttgaatcta ttcggacgaa
     3541 aggtgcagcg ggtaaacaat tggccatggg tgagcgttct cagctggagg cattccagac
     3601 ggcggcgcag caaatctcag cgcaagggct ggattctctg gtgcctttct ggcgcttcta
     3661 tgccgccatt gagagcttcc tggaacctgc cgttagccgc accatcactc aggcttgcca
     3721 gaatggcatt cttgatgagt tcgatggcaa cctgcttaaa acgctgttcc tgatccgcta
     3781 tgtggaaacg ctgaaaagca ccctggataa cctggtcaca ttgtctatcg ataggatcga
     3841 tgccgataaa gttgagttgc gccgccgggt cgaaaaaagt ctcaacacgc ttgaacgcct
     3901 gatgctcatt gcgcgcgttg aagataaata tgtgttcctg accaacgaag agaaagagat
     3961 cgaaaacgag atccgtaacg ttgatgtcga tttctctgcg atcaacaaaa aactggcatc
     4021 gatcatcttt gatgacattc tgaaaagccg taaatatcgt tatccggcta acaagcaaga
     4081 ctttgatatc agccgcttcc tgaacgggca tccattagac ggcgcagtgc ttaacgatct
     4141 ggtggtgaag atcctgaccc ctaaagatcc gacttattcg ttctataaca gcgatgcgac
     4201 ctgtcgccct tatacgtcag aaggcgacgg ctgtattttg attcgtctgc ccgaagaggg
     4261 ccgtacctgg agcgatattg atttagtcgt ccagactgaa aagttcctca aagataacgc
     4321 cgggcaacgt ccggaacagg caaccctgct ctcagaaaaa gcgcgtgaaa acagcaaccg
     4381 ggaaaaatta ctccgtgttc agttggaatc actacttgca gaagcagacg tctgggcgat
     4441 tggcgaacgc ttaccgaaaa aatcctccac gccatcgaac attgtcgatg aagcctgccg
     4501 ttacgtgatt gaaaacacct tcggcaagct gaagatgctg cggcctttta acggtgacat
     4561 ctcccgtgaa attcatgcat tactgacggt tgagaacgac accgaactgg atctcggtaa
     4621 cctcgaagag tccaaccccg acgccatgcg cgaggtagaa acctggatca gcatgaatat
     4681 cgaatacaat aaacctgtgt atttacgcga tattctgaac cattttgcgc gtcgccctta
     4741 tggctggccc gaagacgaag tgaaactgct agtagcccgt ctggcctgca aaggtaaatt
     4801 cagcttcagc cagcaaaaca acaacgtcga gcgaaaacag gcgtgggagt tatttaataa
     4861 cagccgccgc catagcgaat tgcgtctgca taaagttcgc cgtcatgatg aagcgcaggt
     4921 gcgtaaagcc gcgcaaacca tggctgacat cgctcagcag ccgtttaacg aacgggaaga
     4981 gccggcgctg gttgaacata ttcgtcaggt atttgaagag tggaagcaag agctgaacgt
     5041 attccgcgcc aaggcagagg gcggaaacaa tccggggaaa aacgagattg aatccggtct
     5101 gcgcctgctt aatgccattc ttaatgagaa agaagatttt gccctgatcg aaaaagtctc
     5161 atcgctgaaa gatgaacttc tggatttcag cgaagaccgt gaagatttgg tcgacttcta
     5221 ccgtaagcaa ttcgccacct ggcaaaaact gggtgctgcg ctgaatggca gctttaaatc
     5281 taaccgcagc gcgctggaaa aagacgccgc agcggttaaa gcgctgggcg agctggaaag
     5341 catctggcaa atgccggaac cttataagca tctcaatcgc atcacgccgt tgattgaaca
     5401 ggtccagaac gtcaaccatc agttagtcga acagcatcgc cagcacgccc tcgaacgcat
     5461 tgacgcccgc attgaggaaa gccgtcaacg cttgctggaa gcgcacgcca cgtcggagct
     5521 gcaaaacagc gttctgctgc cgatgcaaaa agccagaaaa cgcgctgaag tcagccagtc
     5581 gattccggaa attttggcgg aacagcaaga gacaaaagcg ctgcaaatgg atgcagataa
     5641 aaagattaac ctgtggatcg acgagctgcg taaaaagcaa gaagcacaac tccgggcagc
     5701 aaatgaagct aaacgcgctg ccgactcaga acagacttat gttgtggtgg aaaaaaccgt
     5761 tatccaaccg gtaccgaaaa aaacgcatct ggtgaatgtc gccagtgaga tgcgtaatgc
     5821 caccggtggt gaagttctgg aaacgaccga acaggtggaa aaggcgctcg acacgttacg
     5881 cacaacgctg ctggccgtca ttaaagcagg cgatcgcatt cgccttcagt aactcccatt
     5941 tcagggcagc actctgctgc cctttgcagg attttctatg aataccaata acattaaaaa
     6001 atatgcccca caggcccgta acgacttccg cgatgcggtg atccagaagc taacgacgct
     6061 tgggatcgct gcagataaaa aaggcaattt gcagattgcc gaggccgaaa ccattggcga
     6121 gaccgtgcgt tacggtcagt ttgattaccc gttatcgacc cttccccgcc gcgaacggct
     6181 ggtaaaacgc gcccgtgagc agggttttga ggtgctggtt gagcactgcg cctacacctg
     6241 gtttaaccgc ttatgtgcaa ttcgctatat ggagctacac ggttatcttg agcacggctt
     6301 ccgtatgttg tcccacccgg agacgccgac cgcgtttgag gtgctggatc atgtgccgga
     6361 agtggcagaa gccctgctgc cggaaaataa ggcgcagctg gttgaaatga agctttccgg
     6421 taatcaggac gaagccctgt accgcgaact gctgctgggg cagtgccacg ccctgcacca
     6481 cgcgatgccg ttcctgtttg aagcggtaga tgacgaagcg gaactgctgt tgccggataa
     6541 cctgacccgt accgactcta ttctgcgtgg gctggttgat gatattccgg aagaagactg
     6601 ggagcaggta gaggttatcg gctggctgta tcagttctat atttcggaaa agaaagatgc
     6661 cgtgattggc aaagtggtga agagcgaaga tattcctgcc gccacccagc tgtttacgcc
     6721 aaactggatt gtgcagtatc tggtacaaaa ctccgttggc cgccagtggt tgcagaccta
     6781 cccggactcg ccgctgaaag acaaaatgga gtactacatc gagcctgcgg aacaaacgcc
     6841 ggaagtgcag gcgcagctgg cggcgattac cccagccagc attgaacccg aaagtattaa
     6901 agtgctcgac ccagcctgcg gctccggtca tattttgatt gaagcctata atgtgctgaa
     6961 aaatatctac gaagagcgtg gttatcgcgg gcgtgatatt ccacaactga ttctggaaaa
     7021 taatattttt ggtcttgata tcgacgaccg cgcggcacag ctttccggct ttgcattatt
     7081 aatgatggcg cgtcaggatg accgcagaat atttacccgc gatgtacgtc tgaatattgt
     7141 ctctttgcag gaaagcctgc atctggatat cgccaaactc tggcagcaac tgaatttcca
     7201 ccagcaggta caaaccggca gtatggggga tatgtttgct gaaaataacg cgttaaccca
     7261 aactgacagc gcagaatatc agctgctgat gcgcacgctg aaacgctttg tgaatgcaaa
     7321 aacgctgggc tcactgattc aggtgccgca ggaagaagaa gcggaactga aggtattcct
     7381 ggacgcgttg tatcgcctgg aacaggaagg cgatttccag cagaagacgg cggcaaaagc
     7441 gtttattccg tttattcagc aggcgtggat tttagcgcag cgatatgatg cggtagtggc
     7501 gaatccgccg tatatggggg gtaattatat ggagacagaa cttaagaatt tcgtctcttc
     7561 ttactaccct caaggaaagg cggatcttta ttcttcattt atggtcagat tacttttaca
     7621 attaaaagat aatcgcactt taagcctaat gacccccttt acttggatga atttatcatc
     7681 atttgaagag ctccgaaaaa ttatacttac aaatttcagc attcagtcat tagtacagcc
     7741 tgaatatcat tcattttttg agtcagctta tgtcccaatt tgtgctttta gcatttcaaa
     7801 taccccatta agctggaatg caaaattttt tgatttatca gatttttatg gagaaaaaaa
     7861 tcaagctcca aattttcagt atgcaattaa aaatgacaat aaatgtcatt ggaaatataa
     7921 cagaatcacc acggactttc tatgtactcc cggatatatc attgcttact ctctgcctga
     7981 ttctgcgtta tcttgcttca aaacatccaa aaaacttcat gatgtttgca atctaaaaca
     8041 aggattaatt actggtgata atgaaagata cctaagattc tggcatgaaa tcagctataa
     8101 ctctttcagt ctcaatgaaa aaagaaaaaa aacaaaatgg ttcccatatc aaaaaggtgg
     8161 tgcataccgt aaatggtatg gtaataatga ttatgttgtt gactgggaga atgatggtta
     8221 ttccattaaa aacttttata atgacaaagg taaattacgc tcacgccctc aaaacataca
     8281 attttattgt aaagagggtt taacatggac aagtttaact atttcgtcac tatcgatgag
     8341 atatgtacca aatggatata tttttgatgc aaaaggacct atgtgttttc cgaaatcctc
     8401 tttggatatc tggaatattc ttggctatgc gaatagcaaa gtaatagata tatttctcaa
     8461 acaattagcg cccaccatgg attattctca agggcctgtt ggaaatgtcc cattcaaatt
     8521 taacgatggt gatttgaacg agataataaa agaactcgta aacattcaca aacgtgactg
     8581 ggatgaaaat gaaacatctt ttgagtttaa gagagatatg ttggttcatt tttcaagaga
     8641 tattaacact attaagggta gttttacact aaggcaaggg gaaaataaaa aagcgattaa
     8701 cagaacaaaa tttttagaag aaatgaataa ctctttcttt ataaattgct ttaatctaac
     8761 tgatatttta tctccagaaa ttgaactaaa caaaatcacg ttaacgcatg caactattga
     8821 aattgatatt caaaaaataa tttcatatgc aataggctgc caaatgggac gttactccct
     8881 tgatcgcgaa ggtctggtat acgctcatga aggcaataat ggcttcgccg atcttgtcgc
     8941 cgaaggtgct tataaaagct tcccggctga tagtgacggc attctgccgc taatggatga
     9001 agagtggttt gacgatgacg tcacctctcg cgtcaaggag tttatccgca ccgtttgggg
     9061 cgaagaatat ttgcgcgaaa acctcgattt tatagccgaa gttctcaagc ccaaaaaagg
     9121 cgaatctgcg ctggagacca ttcgtcgcta tctttccacc cagttctgga aagatcatct
     9181 gaaaatgtat aaaaagcgtc caatctactg gctattcagc tccggtaaag agaaagcgtt
     9241 tgagtgcttg gtgtatctgc atcgctataa cgatgccacg ctgtcgagaa tgcgtaccga
     9301 atatgtggtg ccgctgctgg cgcgttatca ggccaatatt gatcgcctga acgatcaact
     9361 tgatgaggct tctggcggtg aatccacacg tctgaaacgc gaacgcgaca gcctgatcaa
     9421 aaaattcagc gaactgcgca gctatgacga tcgcctgcgt cactatgctg atatgagaat
     9481 cagtattgat ctcgacgatg gcgttaaggt taactacggc aagtttggcg atctgctggc
     9541 agatgtcaaa gccatcaccg gcaatgcccc agaggtgatc taaaccagac ggcacgttct
     9601 cctgttgccg ggttctgccc ggtggcaaat accaccggga aacgcgccgc tgctgacatt
     9661 tctccacctc acttcatgat aaaatgcgcc accgtgtcaa aatctccttt tcgcgttttg
     9721 gcgctttctt attcatcgta acaacatggg attgtgaact tgcaaaatca ggactttatt
     9781 gctggcctta aagctaaatt tgccgaacat cgcatcgttt tctggcacga tcccgataaa
     9841 cgttttattg aggaactgga acagctcaag cttgaaagcg tcacgctaat caacatgacc
     9901 cacgagtcac agctggcggt aaaaaaacgc atcgagattg atgagccaga acagcagttc
     9961 ctgctgtggt tcccccatga tgcgccgcct catgaacaag actggctgct ggatatccgc
    10021 ctttacagca gcgaattcca tgccgatttt gccgccatca ccctgaacac gctgggcatt
    10081 ccccagcttg gcctgcgcga gcatattcag cgacgcaagg ccttcttcag cactaaacgc
    10141 acgcaggcgc tgaaaaatct ggcgacagaa caggaagatg aagcctcgct ggataagaaa
    10201 atgattgcgg tgatcgctgg cgcaaagacc gcgaaaaccg aagacatttt gttcaacctg
    10261 attacccagt acgttaacca acaaatagaa gacgacagcg aactggaaaa cacgcaggcg
    10321 atgctgaaac gccacggtct ggactcggta ttgtgggaaa tgctcaacca cgaaatgggc
    10381 taccaggcag aggagccatc gctggaaaac ctgctcctga aactgttttg taccgatctc
    10441 tctgcccagg ccgacccaca gcagcgcgcc tggctggaaa aaaatgtcct gctgacgcca
    10501 tccggcagag catctgccct ggcatttatg gtgacctggc gtgccgatcg tcgctataaa
    10561 gaggcttatg actactgcgc tcagcaaatg caggccgccc tgcacccgga agatcattac
    10621 cgactcagct cgccgtatga tttgcacgaa tgcgaaacca ccctcagcat cgaacaaacc
    10681 attattcatg cgctggtaac acagctgctg gaagagagca ccacgctcga tcgggaagcc
    10741 tttaaaaaac tgctctctga gcgccagagc aaatactggt gtcagacaca accagagtat
    10801 tacgccatct atgacgcatt gcgccaggct gagcggttgc tgaacctgcg caatcgccac
    10861 atcgatggtt tccactacca ggacagcgcc accttctgga aagcctactg cgaagaactg
    10921 ttccgcttcg accaggctta tcgcctgttt aatgaatatg ccttgctggt tcacagcaaa
    10981 ggagcgatga tcctcaagag cctggatgat tatatcgagg cgctctacag caactggtat
    11041 ctggcagagt taagccgtaa ctggaacgaa gtgctggaag cggaaaatcg tatgcaggcg
    11101 tggcaaatcc ctggcgtgcc gcgtcagcag aacttcttca atgaggtggt gaagccacag
    11161 ttccaaaatc cgcaaatcaa acgcgtgttc gtgataattt ccgatgccct gcgttatgaa
    11221 gtggcggagg agctggggaa tcaaatcaat accgagaaac gctttaccgc agaactgcgc
    11281 tcgcagctcg gcgtgctccc cagctacacc caactgggaa tggcggcatt gctgccccat
    11341 gaacaacttt gctatcaacc cggtaacggc gacatcgttt atgctgatgg gctgtcgacc
    11401 tcgggtattc ctaaccgcga taccattctg aagaactata agggaatggc gataaaatcg
    11461 aaggaccttc tggagttaaa aaatcaggaa gggcgagacc ttattcgcga ttacgaagtg
    11521 gtgtatatct ggcataacac gattgatgcc actggcgaca cggcatccac ggaagataaa
    11581 accttcgaag cgtgccgcac ggcggtggct gaactgaaag atttagtcac caaggtgatc
    11641 aaccgcctcc acggcacacg catttttgtt acggcggatc acggtttcct gttccagcaa
    11701 caggcgcttt cggttcagga taaaaccact ctgcaaatta agccggaaaa caccatcaag
    11761 aaccacaaac gctttattat cggccatcag cttcccgccg atgatttttg ctggaaaggg
    11821 aaagtggcgg ataccgcagg cgtgagcgac aacagcgagt tcctgattcc gaaagggatc
    11881 cagcgcttcc atttctctgg cggcgcgcgc ttcgttcatg gcggcaccat gttgcaggag
    11941 gtttgcgttc cggtattgca gataaaagcc ctgcaaaaaa ccgccgcaga aaaacagcca
    12001 cagcgccgcc cggtggatat tgtcgcttac catccgatga ttaagctagt gaacaatatc
    12061 gataaagtga gcctgttgca gacgcatccg gtgggcgaac tttatgaacc gcgtatcctg
    12121 aacatttaca ttgtcgacaa cgccaacaat gtggtctcgg gcaaagagcg catcagcttt
    12181 gacagtgata acaacaccat ggaaaaacgc gtacgcgaag ttacgctgaa gctgattggc
    12241 gctaacttca accgtcgcaa tgagtactgg ttgatactgg aagacgcaca aacggaaacg
    12301 gggtatcaga agtacccggt cattatcgat ctggcgttcc aggatgattt cttctaagtg
    12361 aggcgatatg caaacccatc atgatttacc tgtttcaggc gtatccgcag gggaaattgc
    12421 ctccgagggt tacgatctgg acgccctgct gaaccagcat tttgctggtc gcgtggtgcg
    12481 taaagatctc accaagcaac tcaaggaagg ggcaaacgtc ccggtgtatg tgcttgagta
    12541 tctgctcggc atgtactgcg cctctgacga tgacgatgtg gtcgagcaag ggttgcaaaa
    12601 cgttaagcgt attctggctg ataactatgt gcgcccggat gaagcggaga aagtgaagtc
    12661 gctgatccgc gagcgtggtt cgtacaaaat catcgataaa gtgtcggtga aactgaacca
    12721 gaaaaaagac gtttacgaag cccagctttc taacctcggc atcaaagacg cgctggtgcc
    12781 ctcgcagatg gttaaagaca acgagaagct actgacgggc ggtatctggt gcatgattac
    12841 cgtcaactat ttctttgaag aagggcagaa gacctcaccc ttctcattga tgacgctcaa
    12901 gcctatccag atgccgaata tggatatgga agaggtgttc gatgcgcgta aacactttaa
    12961 ccgtgaccag tggatcgatg tgctgctgcg ctcggtgggt atggagcccg ccaatattga
    13021 gcaacgcacc aaatggcacc ttatcacccg tatgatcccg ttcgtggaga acaactataa
    13081 cgtttgcgag ctggggccgc gtggcaccgg taaaagccat gtgtataaag agtgttctcc
    13141 taactccctg ttagtttccg gcgggcaaac gaccgttgcc aacttgttct acaacatggc
    13201 cagtcgccag atcggcctgg ttggcatgtg ggatgtggta gcgttcgacg aagtcgcggg
    13261 gatcactttc aaagataaag acggcgtgca aatcatgaaa gattacatgg cgtcaggatc
    13321 tttctctcgc ggcagagatt cgattgaagg taaagcgtcg atggttttcg tcggcaacat
    13381 caatcaaagc gtagagactc tcgttaaaac cagccatttg ctggcaccat ttccgactgc
    13441 gatgattgat acagcatttt tcgaccgctt tcatgcctat attcccggtt gggaaatccc
    13501 caaaatgcgc ccggaattct ttaccaaccg ttacgggctg attacggatt atctcgctga
    13561 atatatgcgc gaaatgcgca aacgcagttt ctctgatgcg attgataaat tctttaagct
    13621 gggtaacaac ctcaaccagc gtgacgttat tgccgttcga cgtaccgtgt cggggttgtt
    13681 aaaactcatg catcccgatg gcgcgtacag caaagaagat gtgcgagtct gcctgaccta
    13741 tgcgatggaa gttcgccgcc gcgtgaaaga gcaacttaaa aaactgggcg gtctggagtt
    13801 cttcgatgtg aactttagct acatcgacaa cgaaacgctg gaagagtttt ttgtgagcgt
    13861 accggaacag ggcggcagcg aacttattcc tgccggaatg ccaaagccgg gtgttgtgca
    13921 tctggtcact caggcagaaa gcggcatgac cgggctgtat cgttttgaaa cacagatgac
    13981 tgccggtaat ggtaagcata gtgtatcggg tctgggttca aatacctccg cgaaagaagc
    14041 tatccgcgtc ggtttcgatt acttcaaagg caatttgaat cgggtaagcg cggccgcgaa
    14101 attctccgat catgaatatc accttcatgt cgttgaactg cataatactg gcccaagcac
    14161 cgcaaccagt cttgctgcgc ttatcgcttt atgttcgata ttgctggcaa aaccggtgca
    14221 ggaacagatg gtggtgttgg gcagtatgac gcttggtggg gtaattaacc cggtgcagga
    14281 tcttgccgcc agtttgcagc tcgccttcga cagcggtgca aaacgggttc tgttgccgat
    14341 gtcctcggct atggatattc caacggttcc ggcagagtta tttaccaagt ttcaggtgag
    14401 tttttactca gacccggttg atgctgttta taaggcgctg ggtgtgaatt aacgtagtaa
    14461 ctattttaat gaaccgcaat aactagcata accccttggg gcctctaaac gggtcttgag
    14521 gggttttttg ctgaaacctc aggcatttga gaagcacacg gtcacactgc ttccggtagt
    14581 caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc
    14641 gacgaccggg tcgaatttgc tttcgaattt ctgccattca tccgcttatt atcacttatt
    14701 caggcgtagc accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc
    14761 cctgccactc atcgcaatac tgttgtaatt catttaacat tctgccgaca tggaagccat
    14821 cacagacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
    14881 aatatttgcc catagtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
    14941 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
    15001 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
    15061 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
    15121 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
    15181 tcgccatacg gaactctgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
    15241 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
    15301 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
    15361 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
    15421 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
    15481 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc
    15541 agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg
    15601 tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt
    15661 gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt
    15721 tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg
    15781 agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg
    15841 caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc
    15901 ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta
    15961 cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg
    16021 gccgcggcaa agccgtt
//
