LOCUS       sequence-320169-        4522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Ec86 retron defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG007 (Ec86 retron)
FEATURES             Location/Qualifiers
     source          1..4522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2293..2340
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2560..3219)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3134..3153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3220..3322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(3714..3731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3848..4393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3928..3947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
       61 aatgagtgag ctaacttaca ttaattgcgt tgcgcagctc atgttatgca tgtgcatgaa
      121 aaccactgca taaagcgggc aggcgtggcg gggatacgag cgcgcgccat gtggtatgga
      181 gattggatct attcataact tgatgtataa agtagaaaaa aaagcgggga gattatgaat
      241 aaaaaattta ccgatgagca gcaacaacag cttataggac atctcacaaa gaaaggcttc
      301 tatcgaggag ctaatattaa aataaccatt tttctatgtg gtggtgacgt tgctaatcat
      361 caatcttggc gtcatcaatt atcacaattt ttagcaaagt tcagtgatgt tgatatattt
      421 tatccagaag atctatttga tgatcttttg gctggtcaag ggcagcatag ccttttaagt
      481 ttagaaaata ttctggctga agctgtcgat gtaataattt tatttcctga aagtccgggg
      541 tctttcacag agcttggtgc gttctctaat aatgaaaact taaggagaaa gttgatttgc
      601 attcaagatg caaaatttaa atcaaaacgt agctttatta actatggtcc tgttcgcctg
      661 ttgcgtaagt ttaattcaaa atctgttttg cgttgtagtt caaatgaact aaaagaaatg
      721 tgtgattcat ctattgatgt tgccagaaaa ttacgattat ataaaaaatt aatggcatct
      781 attaagaagg ttaggaaaga aaataaagta tcaaaagata ttggaaatat attatacgca
      841 gagcggtttc tattgccttg tatctattta ctggatagtg tcaactaccg cacactgtgt
      901 gaactagctt ttaaagcgat aaagcaagat gatgttttat ctaaaattat tgttagatcc
      961 gttgtttctc gtctaataaa tgaacgaaaa atacttcaaa tgactgatgg ttatcaggtc
     1021 actgctttgg gggctagcta tgttaggagc gtctttgata gaaagacact tgaccgattg
     1081 cggcttgaga ttatgaattt tgaaaaccgt agaaaatcaa catttaacta tgataagatt
     1141 ccgtatgcgc acccttagcg agaggtttat cattaaggtc aacctctgga tgttgtttcg
     1201 gcatcctgca ttgaatctga gttactgtct gttttccttg ttggaacgga gagcatcgcc
     1261 tgatgctctc cgagccaacc aggaaacccg ttttttctga cgtaagggtg cgcaactttc
     1321 atgaaatccg ctgaatattt gaacactttt agattgagaa atctcggcct acctgtcatg
     1381 aacaatttgc atgacatgtc taaggcgact cgcatatctg ttgaaacact tcggttgtta
     1441 atctatacag ctgattttcg ctataggatc tacactgtag aaaagaaagg cccagagaag
     1501 agaatgagaa ccatttacca accttctcga gaacttaaag ccttacaagg atgggttcta
     1561 cgtaacattt tagataaact gtcgtcatct cctttttcta ttggatttga aaagcaccaa
     1621 tctattttga ataatgctac cccgcatatt ggggcaaact ttatactgaa tattgatttg
     1681 gaggattttt tcccaagttt aactgctaac aaagtttttg gagtgttcca ttctcttggt
     1741 tataatcgac taatatcttc agttttgaca aaaatatgtt gttataaaaa tctgctacca
     1801 caaggtgctc catcatcacc taaattagct aatctaatat gttctaaact tgattatcgt
     1861 attcagggtt atgcaggtag tcggggcttg atatatacga gatatgccga tgatctcacc
     1921 ttatctgcac agtctatgaa aaaggttgtt aaagcacgtg attttttatt ttctataatc
     1981 ccaagtgaag gattggttat taactcaaaa aaaacttgta ttagtgggcc tcgtagtcag
     2041 aggaaagtta caggtttagt tatttcacaa gagaaagttg ggataggtag agaaaaatat
     2101 aaagaaatta gagcaaagat acatcatata ttttgcggta agtcttctga gatagaacac
     2161 gttaggggat ggttgtcatt tattttaagt gtggattcaa aaagccatag gagattaata
     2221 acttatatta gcaaattaga aaaaaaatat ggaaagaacc ctttaaataa agcgaagacc
     2281 taatcgcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     2341 ctgaaacctc aggcatttga gaagcacacg gtcacactgc ttccggtagt caataaaccg
     2401 gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc gacgaccggg
     2461 tcgaatttgc tttcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc
     2521 accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc
     2581 atcgcaatac tgttgtaatt catttaacat tctgccgaca tggaagccat cacagacggc
     2641 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     2701 catagtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     2761 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
     2821 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     2881 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     2941 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca tcgccatacg
     3001 gaactctgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     3061 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     3121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     3181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     3241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     3301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
     3361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
     3421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
     3481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
     3541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
     3601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
     3661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
     3721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
     3781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
     3841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
     3901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
     3961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     4021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     4081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     4141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     4201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     4261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     4321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     4381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     4441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     4501 atgttagtca tgccccgcgc cc
//
