LOCUS       sequence-320174-        6663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the dsr2 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 6663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG023 (dsr2)
FEATURES             Location/Qualifiers
     source          1..6663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3934..3981
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(4201..4860)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4775..4794
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4861..4963)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(5355..5372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5489..6034)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5569..5588)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggcggggcg agcattgcgc gtcggtgtta ccaattctat atggacattg agcaattcaa
       61 atataataaa ggttgggtat atttcgtcct caacgatgtc aaaaactgca aaagcgtatt
      121 ataattcaga tcattttcag accacctatt ttaatcatgc atgcaaaatg gaatatgtga
      181 tgacaaataa aaacaaaatc aaaccattat taaataatat atccgctcgc ctttgggatg
      241 gtcgtgcagc tatattgata ggagctgggt tcagtcggaa tgcaaagcca ttaacaagca
      301 aggcaagaaa gtttccaatg tggaacgact taggtgacat tttttatgaa agtgtttact
      361 gcaaaaaaaa cgacaataga tattcaaatg tattgaagct aggagatgaa gttcaggctg
      421 catttggtag agcgacactt gataaattaa tcatggatca tgttccagat aaagaatatg
      481 aaccatccaa attacatgtt tcccttcttt ccttgccgtg gattgatgtt tttacgacta
      541 attatgatac attacttgag cgagcaagtg ttaatgtcga ctccagaaaa tatgacattg
      601 tccttaataa aaatgattta atgaatgctg aaagaccaag aattataaaa ctgcatggta
      661 gcttcccatc agaaaggccc ttcatagtta cggaggaaga ttacagaaag tatcctttag
      721 aaaattctcc ttttgtgaat accgttcaac aatcattgat tgagaatact ctatgtctga
      781 taggattttc gggtgacgat cctaacttct taaattggat tggttggata agagataatc
      841 ttggcacaga aaattcaccc aaaatatact tgatcggtct tttttcattt aatgaagcac
      901 aacgtaagct tttagaaaaa agaaatattt ccattgttga tttaagtttt ctaggtgatt
      961 ttggcaagga tcattatcta gcacaccaac gctttatcca attcttatac gaatcaaaaa
     1021 atcgagacaa cctaatagag tggccaatag aaaccaatta tgacagaatt gtttttaatg
     1081 atggcattga attaaaaact gagaaaatta aaaagtgtat cttagaatgg gctcagtcaa
     1141 gacaatcata cccgaactgg cttattttgc cggaatcaaa cagaagtaat ttatggcaaa
     1201 acactataga ttggttatct gttgctaatt atgatgtcgc ttgggatggt tctgatgatc
     1261 ttgattttgg atatgaaatt acatggcgac taaataaagc tttgctacca attttcaatg
     1321 atacatcaga attcttattt aagttgattg aaaaatatga gatcaattac gtttcgggga
     1381 taaataataa aatcattgac tttgatgaaa aatactctca tataaccctc agtttaatga
     1441 gattctgtcg acaagaaaac cttattgata aatggaagaa tctaaacgat ttattaattc
     1501 aaaatcttga tcgattaaca ccagaggtaa aatctgatta ttattatgaa aatatattat
     1561 tttcatactt caatttaaac ttcgatgaag ccagaaacaa actctccaac tgggaaacga
     1621 ataaactcct cccccatcat gaaataaaaa gagcaggatt acttgccgaa tttggaatgc
     1681 ttgatgaagc aatcaatctt cttgaagaaa ctttatctac gattcgaaga aacagtttgc
     1741 tttcatctag aaacattgac tattccagtg aatctcaaga agcatatgga atctatattt
     1801 tgcgaatgtt taaacggagt ttgcgtttag atagcaaaga tgacgattat tcatctgagt
     1861 ataactcgcg gttggctaca ttatcacaat atcgcagcga tcctgaaaac gaaataaaat
     1921 acctagaaat taaactagag tcactaccag gtaccttcaa gaataccaat gacacggatt
     1981 tcgatcttaa caaaagaacg gtgaccactt atttaggagg aagcccaaca gaagtgaggt
     2041 cattagatgc ttttagtttc tttctactgg cagaggaact tggcctccct ttccacatac
     2101 caggaatgaa catttttagt ggaatagttg agaatgcagc tcgacatatt tatcaatact
     2161 ctccagagtg ggctattttt tcaatattta gaacatttaa caaggataag gccaagagtc
     2221 tattcaatcg aaatagaatt tcgtctcttg agcgaaaaaa ggttgaagat ttatttgatg
     2281 gatactacaa aaaatatgag caaattatca caaaaaaaat agaagataga ttaaacgata
     2341 aacttgagat agaaatttct acgctatcaa tcattcctga aattctttcc cggctagtta
     2401 caaaagtatc atttaataaa aagaaagaca ttattcacct tttgcttaaa ctgtttaact
     2461 cggataattt tcatcaatac atggagacta aagatctatt aaagcgcact atttccaatt
     2521 tgagcgactt acaaaagatc tcactaatag atattttcat tgatttcccc tccgcgcctc
     2581 ccaataccca attacatatg ggtcaaagat acaacttcct tactccattt gaatgtctat
     2641 taggggttac aataaccccc ccaaaagaaa actctaaaaa aatcgcatct gcaaaattaa
     2701 aaaaagatat aaacgattta aaaagtgata atttagactt gaggaaagct gtatcacaaa
     2761 agctcataac attatataac ctagaaatgc ttaacaaatc tgacacgact aaacttataa
     2821 aaaacctttg gtcaaagcgt gataactttg gattcccaat aggcagtggt tactataaat
     2881 ttttctttat aaacaacctt aacccagata atgaaaatat agccgacaaa ttcatttcta
     2941 taattaaaac atacaaattt cctgtgcaag aaggaaaaag agttagtatt acaggtgggt
     3001 tagatgagta ttgtactgaa ctcaatggag cgctacacca tataagtctt ccagagaaaa
     3061 ccctatctga aataatttca aaaatacatg actggtatgt caaggatcgg gcctggcttg
     3121 aaaaaagaga tgatttagcc aaggagttca ctcttagatt cagaaatatc acaaatatca
     3181 taacgacaat tttagaacac cataaggaca aattacatgc tgaatctata aatgaaatat
     3241 caagcctact agataaaatg aaagaagaca agatacctgt aaactcagca gtaacaatgc
     3301 tttgtctgaa aaataaaagc acttacctcg agagaataaa agatatagag aatggactat
     3361 atagctttaa taaagatgat gttattgaag ctatcaactc aacttatgtc tttattagaa
     3421 acaatgaatt tccactaacc atcattcaag ctatcagcga taaaatcgca tgggatagaa
     3481 accctcgcct tcctgattgc tacaatttaa ttgcatatat aattaactcg tgtgaattta
     3541 ctcttccaga ttatttaata gagaaaatcc ttcgagggct ggcatatcaa ataaacattg
     3601 atgatagaga ttttgttgat aacaatgaat atttgaatca ccttgagaaa aaacttagtg
     3661 caacaaagct ggctgcttct atgtttagaa aaaatgaaac actaggtatt gaccaacctt
     3721 ctatcattca agagtggaaa aacatgtgca actctagaaa tgagttcgat gaaattagga
     3781 atgaatggaa caacaatata taaataaagg aagaacaccc aatttatatt gggtgttctg
     3841 ttcacgaaac ccttttacca taatcgaatg gcaatataaa ttgagattga aatttattct
     3901 catctaatta atcagcccac cattgcgcaa taactagcat aaccccttgg ggcctctaaa
     3961 cgggtcttga ggggtttttt gctgaaacct caggcatttg agaagcacac ggtcacactg
     4021 cttccggtag tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc
     4081 tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat
     4141 tatcacttat tcaggcgtag caccaggcgt ttaagggcac caataactgc cttaaaaaaa
     4201 ttacgccccg ccctgccact catcgcaata ctgttgtaat tcatttaaca ttctgccgac
     4261 atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     4321 gccttgcgta taatatttgc ccatagtgaa aacgggggcg aagaagttgt ccatattggc
     4381 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
     4441 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     4501 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     4561 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     4621 accgtctttc atcgccatac ggaactctgg atgagcattc atcaggcggg caagaatgtg
     4681 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     4741 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     4801 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     4861 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     4921 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca
     4981 aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa
     5041 gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt
     5101 actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct
     5161 gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc
     5221 agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt
     5281 gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg
     5341 atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg
     5401 gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg
     5461 gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc
     5521 acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg
     5581 cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg
     5641 tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag
     5701 gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc
     5761 ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca
     5821 gcagccactg gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta
     5881 aactgaaagg acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga
     5941 gttggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga
     6001 gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa
     6061 atatttctag atttcagtgc aatttatctc ttcaaatgta gcacctgaag tcagccccat
     6121 acgatataag ttgtaattct catgttagtc atgccccgcg cccaccggaa ggagctgact
     6181 gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt
     6241 acattaattg cgttgcgcag taagacaagg gttgagcagg ctactaatcg ttacacaggc
     6301 taacaaaggc atattaagac gatttgtagc gctgtaacct tgaaaattat gtacaagcgc
     6361 cccgcattac gtcgttttaa aggccatcgg attcaggccc gacgcggctt cacgcgatta
     6421 taaccgtgaa aaatcccccc cgcatagaac ctgaattatc cccgccgccg cgcagaactg
     6481 acagcgcttc agaaccgtta accctctcag aaatcccgct tttttactgt aaaaaaccat
     6541 gcataaggtg catggttttg catgcgtttc accgacactg aatcccccgc cagcgccagc
     6601 agtagcgtgc cctgaggccg ttaatgcacc cgtattaaaa gcgccctgtt aagcgagcag
     6661 gcg
//
