LOCUS       sequence-320192-       10373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for TAZ with N-terminal FLAG and His 
            tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10373)
  AUTHORS   Yang Z, Nakagawa K, Sarkar A, Maruyama J, Iwasa H, Bao Y, 
            Ishigami-Yuasa M, Ito S, Kagechika H, Hata S, Nishina H, Abe S, 
            Kitagawa M, Hata Y
  TITLE     Screening with a novel cell-based assay for TAZ activators 
            identifies a compound that enhances myogenesis in C2C12 cells and 
            facilitates muscle repair in the muscle injury model.
  JOURNAL   Mol Cell Biol. 2014 Feb 18.
  PUBMED    24550007
REFERENCE   2  (bases 1 to 10373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2014 Feb 18."
COMMENT     Sequence Label: pLenti-EF-FH-TAZ-ires-blast
FEATURES             Location/Qualifiers
     source          1..10373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        178..1340
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          409..1331
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1288..1308
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1374..1397
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1404..1421
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    3298..3873
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3467..3484)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3694..3713
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3919..4317
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(4372..4392)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4377..4706
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4557..4692
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4718..4765
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4784..5158
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             5251..5484
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5556..5677
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5593..5612)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5647..5666
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5717..5852
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5872..5891)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5873..5891)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5901..5918)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5901..5917)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5910..5932)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6059..6514
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6146..6165)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6356..6377
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6540..6644
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6645..6713,6714..7505)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6863..6882)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7676..8264
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8165..8184
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8418..8435
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8552..8573
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8588..8593,8594..8611,8612..8618)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8626..8642
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8631..8653
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8650..8666
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8650..8666
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8685..8705
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8687..8705
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8733..8959
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8960..9140
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9187..9312
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9805..10038
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10223..10267
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
       61 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
      121 ccattcgatt agtgaacgga tctcgacggt atcgattaat taattgctag ctcgtgaggc
      181 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
      241 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
      301 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
      361 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
      421 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
      481 tccacctggc tccagtacgt gattcttgat cccgagctgg agccaggggc gggccttgcg
      541 ctttaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
      601 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
      661 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
      721 cgggccagga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
      781 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
      841 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
      901 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
      961 ggccgcttcc cggccctgct ccagggggct caaaatggag gacgcggcgc tcgggagagc
     1021 gggcgggtga gtcacccaca caaaggaaag gggcctttcc gtcctcagcc gtcgcttcat
     1081 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctgg agcttttgga
     1141 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     1201 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     1261 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     1321 ttccatttca ggtgtcgtga agcttgttaa cgtttaaact agccggggcc atggactaca
     1381 aagacgatga cgacaaggaa ttgcaccatc atcatcatca tgaattcacg cgctcaggct
     1441 cagcttcgct gcccgcccag aagatgaatc cggcctcggc gccccctccg ctcccgccgc
     1501 ctgggcagca agtgatccac gtcacgcagg acctagacac agacctcgaa gccctcttca
     1561 actctgtcat gaatccgaag cctagctcgt ggcggaagaa gatcctgccg gagtctttct
     1621 ttaaggagcc tgattcgggc tcgcactcgc gccagtccag caccgactcg tcgggcggcc
     1681 acccggggcc tcgactggct gggggtgccc agcatgtccg ctcgcactcg tcgcccgcgt
     1741 ccctgcagct gggcaccggc gcgggtgctg cgggtagccc cgcgcagcag cacgcgcacc
     1801 tccgccagca gtcctacgac gtgaccgacg agctgccact gcccccgggc tgggagatga
     1861 ccttcacggc cactggccag aggtacttcc tcaatcacat agaaaaaatc accacatggc
     1921 aagaccctag gaaggcgatg aatcagcctc tgaatcatat gaacctccac cctgccgtca
     1981 gttccacacc agtgcctcag aggtccatgg cagtatccca gccaaatctc gtgatgaatc
     2041 accaacacca gcagcagatg gcccccagta ccctgagcca gcagaaccac cccactcaga
     2101 acccacccgc agggctcatg agtatgccca atgcgctgac cactcagcag cagcagcagc
     2161 agaaactgcg gcttcagaga atccagatgg agagagaaag gattcgaatg cgccaagagg
     2221 agctcatgag gcaggaagct gccctctgtc gacagctccc catggaagct gagactcttg
     2281 ccccagttca ggctgctgtc aacccaccca cgatgacccc agacatgaga tccatcacta
     2341 ataatagctc agatcctttc ctcaatggag ggccatatca ttcgagggag cagagcactg
     2401 acagtggcct ggggttaggg tgctacagtg tccccacaac tccggaggac ttcctcagca
     2461 atgtggatga gatggataca ggagaaaacg caggacaaac acccatgaac atcaatcccc
     2521 aacagacccg tttccctgat ttccttgact gtcttccagg aacaaacgtt gacttaggaa
     2581 ctttggaatc tgaagacctg atccccctct tcaatgatgt agagtctgct ctgaacaaaa
     2641 gtgagccctt tctaacctgg ctgtaatcac taccattgta acttggatgt agccatgacc
     2701 ttacatttcc tgggcctctt ggaaaaagtg atggagcaga gcaagtctgc aggtgcacca
     2761 cttcccgcct ccatgactcg tgctccctcc tttttatgtt gccagtttaa tcattgcctg
     2821 gttttgattg agagtaactt aagttaaaca taaataaata ttctattttc attttctgca
     2881 agcctgcgtt cttgtgacag attatacaga attgtgtctg caggattgat tatgcagaat
     2941 acttttctct ttcttctctg ctgccccatg gctaagcttt atgggtgtta attgaaattt
     3001 atacaccaat tgattttaaa ccataaaaag ctgaccacag gcagttactt ctgagggcat
     3061 cttggtccag gaaatgtgca caaaattcga cctgatttac agtttcaaaa actgtattga
     3121 tgacagtagt accaaatgct ttaaaaacta tttaacttga gctttaaaaa tcattgtatg
     3181 gatagtaaaa ttctactgta tggaatacaa tgtaattttg aatccaaaaa aaaaaaaaaa
     3241 aaaactcgac ccgggcggcc gcactcgaca attctgcatc tagggcggcc aattccgccc
     3301 ctctcccccc cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     3361 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     3421 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     3481 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     3541 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     3601 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     3661 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     3721 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     3781 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     3841 gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccggg atcctctagc
     3901 aaggtgagga actaaaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa
     3961 agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca
     4021 gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga
     4081 ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact
     4141 tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga
     4201 caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag
     4261 ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagca
     4321 cttcgtggcg gcccgcgtac cggttagtaa tgagtttgga attaattctg tggaatgtgt
     4381 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4441 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     4501 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     4561 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     4621 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     4681 tttttggagg cctaggcttt tgcaaaaagc tccccctgtt gacaattaat catcggcata
     4741 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     4801 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     4861 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     4921 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     4981 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     5041 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     5101 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     5161 cgtgctacga gatttaaatg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     5221 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     5281 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     5341 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5401 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5461 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     5521 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     5581 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5641 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     5701 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5761 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5821 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     5881 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     5941 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     6001 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     6061 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6121 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6181 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6241 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6301 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6361 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6421 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6481 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     6541 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6601 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6661 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6721 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6781 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6841 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6901 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6961 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7021 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7081 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7141 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7201 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7261 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7321 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7381 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7441 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7501 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7561 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7621 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7681 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7741 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7801 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7861 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7921 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7981 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8041 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8101 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8161 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8221 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8281 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8341 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8401 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     8461 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     8521 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     8581 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     8641 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     8701 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     8761 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     8821 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     8881 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     8941 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     9001 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9061 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9121 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     9181 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     9241 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     9301 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     9361 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     9421 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     9481 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     9541 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     9601 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     9661 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     9721 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     9781 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     9841 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     9901 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     9961 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
    10021 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
    10081 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
    10141 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
    10201 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
    10261 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
    10321 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tag
//
