LOCUS       sequence-320205-        7267 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing full-length CX3CL1 (fractalkine) in 
            photoreceptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Wang SK, Xue Y, Rana P, Hong CM, Cepko CL
  TITLE     Soluble CX3CL1 gene therapy improves cone survival and function in 
            mouse models of retinitis pigmentosa.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 May 14;116(20):10140-10149. doi: 
            10.1073/pnas.1901787116. Epub 2019 Apr 29.
  PUBMED    31036641
REFERENCE   2  (bases 1 to 7267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1901787116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-05-14- 14"; volume: "116"; issue: "20"; pages: 
            "10140-10149"
COMMENT     Sequence Label: AAV-hRedOpsin-fCX3CL1
FEATURES             Location/Qualifiers
     source          1..7267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   248..377
                     /label=AAV2 ITR
     regulatory      2524..2533
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3777..3797)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4195..4206)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4273..4290)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4279..4486
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4574..4703
                     /label=AAV2 ITR
     primer_bind     complement(4722..4739)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4722..4738)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4731..4753)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4880..5335
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4967..4986)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5177..5198
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5362..5466
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5467..5535,5536..6327)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVVMVTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5685..5704)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6498..7086
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6987..7006
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggctg cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc
      301 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc
      361 catcactagg ggttccttgt agttaatgat taacccgcca tgctacttat ctacgtagcc
      421 atgctctagg aagatcggaa ttcgccctta agctagccta cagcagccag ggtgagatta
      481 tgaggctgag ctgagaatat caagactgta ccgagtaggg ggccttggca agtgtggaga
      541 gcccggcagc tggggcagag ggcggagtac ggtgtgcgtt tacggacctc ttcaaacgag
      601 gtaggaaggt cagaagtcaa aaagggaaca aatgatgttt aaccacacaa aaatgaaaat
      661 ccaatggttg gatatccatt ccaaatacac aaaggcaacg gataagtgat ccgggccagg
      721 cacagaaggc catgcacccg taggattgca ctcagagctc ccaaatgcat aggaatagaa
      781 gggtgggtgc aggaggctga ggggtgggga aagggcatgg gtgtttcatg aggacagagc
      841 ttccgtttca tgcaatgaaa agagtttgga gacggatggt ggtgactgga ctatacactt
      901 acacacggta gcgatggtac actttgtatt atgtatattt taccacgatc tttttaaagt
      961 gtcaaaggca aatggccaaa tggttccttg tcctatagct gtagcagcca tcggctgtta
     1021 gtgacaaagc ccctgagtca agatgacagc agcccccata actcctaatc ggctctcccg
     1081 cgtggagtca tttaggagta gtcgcattag agacaagtcc aacatctaat cttccaccct
     1141 ggccagggcc ccagctggca gcgagggtgg gagactccgg gcagagcaga gggcgctgac
     1201 attggggccc ggcctggctt gggtccctct ggcctttccc caggggccct ctttccttgg
     1261 ggctttcttg ggccgccact gctcccgctc ctctcccccc atcccacccc ctcaccccct
     1321 cgttcttcat atccttctct agtgctccct ccactttcat ccacccttct gcaagagtgt
     1381 gggaccacaa atgagttttc acctggcctg gggacacacg tgcccccaca ggtgctgagt
     1441 gactttctag gacagtaatc tgctttaggc taaaatggga cttgatcttc tgttagccct
     1501 aatcatcaat tagcagagcc ggtgaaggtg cagaacctac cgcctttcca ggcctcctcc
     1561 cacctctgcc acctccactc tccttcctgg gatgtggggg ctggcacacg tgtggcccag
     1621 ggcattggtg ggattgcact gagctgggtc attagcgtaa tcctggacaa gggcagacag
     1681 ggcgagcgga gggccagctc cggggctcag gcaaggctgg gggcttcccc cagacacccc
     1741 actcctcctc tgctggaccc ccacttcata gggcacttcg tgttctcaaa gggcttccaa
     1801 atagcatggt ggccttggat gcccagggaa gcctcagagt tgcttatctc cctctagaca
     1861 gaaggggaat ctcggtcaag agggagaggt cgccctgttc aaggccaccc agccagctca
     1921 tggcggtaat gggacaaggc tggccagcca tcccaccctc agaagggacc cggtggggca
     1981 ggtgatctca gaggaggctc acttctgggt ctcacattct tggatccggt tccaggcctc
     2041 ggccctaaat agtctccctg ggctttcaag agaaccacat gagaaaggag gattcgggct
     2101 ctgagcagtt tcaccaccca ccccccagtc tgcaaatcct gacccgtggg tccacctgcc
     2161 ccaaaggcgg acgcaggaca gtagaaggga acagagaaca cataaacaca gagagggcca
     2221 cagcggctcc cacagtcacc gccaccttcc tggcggggat gggtggggcg tctgagtttg
     2281 gttcccagca aatccctctg agccgccctt gcgggctcgc ctcaggagca ggggagcaag
     2341 aggtgggagg aggaggtcta agtcccaggc ccaattaaga gatcaggtag tgtagggttt
     2401 gggagctttt aaggtgaaga ggcccgggct gatcccacag gccagtataa agcgccgtga
     2461 ccctcaggtg atgcgccagg gccggctgcc gtcggggaca gggctttcca tagcggccgc
     2521 gccgccacca tggctccctc gccgctcgcg tggctgctgc gcctggccgc gttcttccat
     2581 ttgtgtactc tgctgccggg tcagcacctc ggcatgacga aatgcgaaat catgtgcgac
     2641 aagatgacct cacgaatccc agtggctttg ctcatccgct atcagctaaa ccaggagtcc
     2701 tgcggcaagc gtgccattgt cctggagacg acacagcaca gacgcttctg tgctgacccg
     2761 aaggagaaat gggtccaaga cgccatgaag catctggatc accaggctgc tgccctcact
     2821 aaaaatggtg gcaagtttga gaagcgggtg gacaatgtga cacctgggat caccttggcc
     2881 actaggggac tgtccccatc tgccctgaca aagcctgaat ccgccacatt ggaagacctt
     2941 gctttggaac tgactactat ttcccaggag gccaggggga ccatggggac ttcccaagag
     3001 ccaccggcag cagtgaccgg atcatctctc tcaacttccg aggcacagga tgcagggctt
     3061 acggctaagc ctcagagcat tggaagtttt gaggcggctg acatctccac caccgtttgg
     3121 ccgagtcctg ctgtctacca atctggatct agctcctggg ctgaggaaaa agctactgag
     3181 tccccctcca ctacagcccc atctcctcag gtgtccacta cttcaccttc aaccccagag
     3241 gaaaatgttg ggtccgaagg ccaaccccca tgggtccagg gacaggacct cagtccagag
     3301 aagtctctag ggtctgagga gataaaccca gttcatactg ataatttcca ggagaggggg
     3361 cctggcaaca cagtccaccc ctcagtggct cccatctcct ctgaagagac ccccagccca
     3421 gagctggtgg cctcgggcag ccaggctcct aagatagagg aacccatcca tgccactgca
     3481 gatccccaga aactgagtgt gcttatcact cctgtccccg acacccaggc agccacaagg
     3541 aggcaggcag tggggctact ggctttcctt ggtcttcttt tctgcctagg ggtggccatg
     3601 tttgcttacc agagccttca gggctgtccc cgcaaaatgg cgggggaaat ggtagaaggc
     3661 ctccgctacg tcccccgtag ctgtggcagt aactcatacg tcctggtgcc agtgtgaacc
     3721 ggtaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3781 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3841 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3901 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3961 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4021 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4081 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     4141 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4201 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4261 cttcgagatc tgcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     4321 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     4381 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     4441 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggactcg agttaagggc
     4501 gaattcccga ttaggatctt cctagagcat ggctacgtag ataagtagca tggcgggtta
     4561 atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     4621 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     4681 tcagtgagcg agcgagcgcg cagccttaat taacctaatt cactggccgt cgttttacaa
     4741 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4801 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4861 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4921 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4981 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5041 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5101 gatggttcac gtagtgggcc atcgccccga tagacggttt ttcgcccttt gacgctggag
     5161 ttcacgttcc tcaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5221 gtctattctt ttgatttata agggattttt ccgatttcgg cctattggtt aaaaaatgag
     5281 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttat aatttcaggt
     5341 ggcatctttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5401 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5461 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5521 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5581 ggtgcacgag tgggttacat cgaactggat ctcaatagtg gtaagatcct tgagagtttt
     5641 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5701 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5761 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5821 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5881 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5941 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     6001 acgatgcctg tagtaatggt aacaacgttg cgcaaactat taactggcga actacttact
     6061 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     6121 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6181 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6241 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6301 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6361 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6421 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6481 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6541 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6601 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6661 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6721 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6781 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6841 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6901 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6961 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7021 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7081 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg cggttttgct
     7141 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7201 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7261 gcggaag
//
