LOCUS       sequence-320240-       10305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian CG-to-GC base editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, 
            Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, 
            Adamson B, Liu DR
  TITLE     Efficient C*G-to-G*C base editors developed using CRISPRi screens, 
            target-library analysis, and machine learning.
  JOURNAL   Nat Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z.
  PUBMED    34183861
REFERENCE   2  (bases 1 to 10305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z."
COMMENT     Sequence Label: pCMV_RBMX-eA3A-UdgX-nCas9
FEATURES             Location/Qualifiers
     source          1..10305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             694..714
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3397..3420,3421..3423,3424..7497)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3397..7497
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7540..7560
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7569..7586
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7609..7626)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7615..7839
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7910..7926)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7910..7926)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7923..7945)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7934..7950
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7958..7964,7965..7982,7983..7988))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8003..8024
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8141..8158)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8312..8900)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8392..8411)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9071..9862,9863..9931))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9694..9713
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9932..10036)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10115..10134)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtcatggtt
      721 gaagcagatc gcccaggaaa gctcttcatt ggtgggctta atacggaaac aaatgagaaa
      781 gctcttgaag cagtatttgg caaatatgga cgaatagtgg aagtactctt gatgaaagac
      841 cgtgaaacca acaaatcaag aggatttgct tttgtcacct ttgaaagccc agcagacgct
      901 aaggatgcag ccagagacat gaatggaaag tcattagatg gaaaagccat caaggtggaa
      961 caagccacca aaccatcatt tgaaagtggt agacgtggac cgcctccacc tccaagaagt
     1021 agaggccctc caagaggtct tagaggtgga agaggaggaa gtggaggaac caggggacct
     1081 ccctcacggg gaggacacat ggatgacggt ggatattcca tgaattttaa catgagttct
     1141 tccaggggac cactcccagt aaaaagagga ccaccaccaa gaagtggggg tcctcctcct
     1201 aagagatctg caccttcagg accagttcgc agtagcagtg gaatgggagg aagagctcct
     1261 gtatcacgtg gaagagatag ttatggaggt ccacctcgaa gggaaccgct gccctctcgt
     1321 agagatgttt atttgtcccc aagagatgat gggtattcta ctaaagacag ctattcaagc
     1381 agagattacc caagttctcg tgatactaga gattatgcac caccaccacg agattatact
     1441 taccgtgatt atggtcattc cagttcacgt gatgactatc catcaagagg atatagcgat
     1501 agagatggat atggtcgtga tcgtgactat tcagatcatc caagtggagg ttcctacaga
     1561 gattcatatg agagttatgg taactcacgt agtgctccac ctacacgagg gcccccgcca
     1621 tcttatggtg gaagcagtcg ctatgatgat tacagcagct cacgtgacgg atatggtgga
     1681 agtcgagaca gttactcaag cagccgaagt gatctctact caagtggtcg tgatcgggtt
     1741 ggcagacaag aaagagggct tcccccttct atggaaaggg ggtaccctcc tccacgtgat
     1801 tcctacagca gttcaagccg cggagcacca agaggtggtg gccgtggagg aagccgatct
     1861 gatagagggg gaggcagaag cagatactct ggaggatcta gcggaggctc ctctggctct
     1921 gagacacctg gcacaagcga gagcgcaaca cctgaaagca gcgggggcag cagcgggggg
     1981 tcagaggcaa gcccagcaag cggcccacgg cacctgatgg acccccacat tttcaccagc
     2041 aacttcaaca acggaatcgg acgacacaag acctacctgt gctatgaggt ggagaggctg
     2101 gacaacggca catccgtgaa gatggatcag cacagaggct tcctgcacgg ccaggccaag
     2161 aatctgctgt gcggcttcta cggccggcac gcagagctgc gctttctgga cctggtgcca
     2221 tctctgcagc tggatcccgc ccagatctat agggtgacct ggttcatcag ctggtcccct
     2281 tgctttagct ggggatgtgc aggagaggtg agggccttcc tgcaggagaa cacacacgtg
     2341 aggctgagaa tctttgccgc ccggatctac gactatgatc ccctgtacaa ggaggccctg
     2401 cagatgctga gggacgcagg agcccaggtg tctatcatga cctatgatga gttcaagcac
     2461 tgctgggaca catttgtgga tcaccagggc tgtccctttc agccttggga cggcctggac
     2521 gagcattccc aggcactgag cggacggctg agggcaattc tgcagaacca gggcaattct
     2581 ggcggatcta gcggaggatc ctctggcagc gagacaccag gaacaagcga gtcagcaaca
     2641 ccagagagca gtggcggcag cagcggcggc agcatggccg gagcacagga ttttgtccca
     2701 catactgccg atctggctga gctggctgcc gccgctggag agtgtagagg atgcgggctg
     2761 taccgggatg ccacacaggc cgtgttcgga gcaggcggca ggagcgcccg catcatgatg
     2821 atcggcgagc agccaggcga taaggaggac ctggccggcc tgccatttgt gggaccagca
     2881 ggccggctgc tggacagagc cctggaggcc gccgacatcg atagggacgc cctgtacgtg
     2941 accaacgccg tgaagcactt caagtttaca cgcgccgcag gaggcaagag gagaatccac
     3001 aagaccccct ctcggacaga ggtggtggcc tgcagacctt ggctgatcgc cgagatgacc
     3061 agcgtggagc cagatgtggt ggtgctgctg ggagcaacag cagcaaaggc cctgctgggc
     3121 aatgacttca gggtgaccca gcacagggga gaggtgctgc acgtggacga tgtgccaggc
     3181 gatcctgccc tggtggcaac cgtgcaccct agctccctgc tgagaggccc aaaggaggag
     3241 cgggaatccg ccttcgctgg gctggtggac gacctgagag tggctgccga tgtgagacct
     3301 agcggtggta gttctggcgg tagctcagga tctgaaactc ccggcacatc tgaaagcgcg
     3361 actcccgaat catctggtgg atcttcaggt ggaagtgaca agaagtacag catcggcctg
     3421 gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc
     3481 aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga
     3541 gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga
     3601 agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag
     3661 atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag
     3721 gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac
     3781 gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc
     3841 gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg
     3901 atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg
     3961 cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag
     4021 gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg
     4081 cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc
     4141 cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac
     4201 acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg
     4261 tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac
     4321 accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac
     4381 caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag
     4441 attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag
     4501 gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg
     4561 ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc
     4621 atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt
     4681 tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc
     4741 tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc
     4801 gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag
     4861 agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc
     4921 aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac
     4981 gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg
     5041 gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc
     5101 aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc
     5161 tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat
     5221 gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga
     5281 gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag
     5341 cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc
     5401 atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc
     5461 aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag
     5521 aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc
     5581 agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa
     5641 gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc
     5701 acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa
     5761 gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag
     5821 aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac
     5881 atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac
     5941 gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac
     6001 gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc
     6061 aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc
     6121 gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag
     6181 cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg
     6241 atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat
     6301 ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg
     6361 aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg
     6421 tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc
     6481 ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag
     6541 attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc
     6601 ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg
     6661 ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct
     6721 atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag
     6781 aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg
     6841 gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg
     6901 gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa
     6961 gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc
     7021 cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc
     7081 tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc
     7141 gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc
     7201 atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa
     7261 gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc
     7321 atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc
     7381 accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac
     7441 cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggtgactct
     7501 ggcggctcaa aaagaaccgc cgacggcagc gaattcgagc ccaagaagaa gaggaaagtc
     7561 taaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc
     7621 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     7681 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     7741 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     7801 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     7861 gctggggctc gataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     7921 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7981 agtgtaaagc ctagggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8041 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8101 cgggaagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8161 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8221 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8281 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8341 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8401 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8461 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8521 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8581 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8641 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8701 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     8761 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8821 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8881 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacactcagt
     8941 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9001 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9061 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9121 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9181 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9241 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9301 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9361 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9421 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9481 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9541 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9601 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9661 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9721 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9781 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9841 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9901 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9961 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10021 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
    10081 atcgatctcc cgatccccta gggtcgactc tcagtacaat ctgctctgat gccgcatagt
    10141 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
    10201 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
    10261 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgtt
//
