LOCUS       sequence-320241-       11340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian CG-to-GC base editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11340)
  AUTHORS   Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, 
            Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, 
            Adamson B, Liu DR
  TITLE     Efficient C*G-to-G*C base editors developed using CRISPRi screens, 
            target-library analysis, and machine learning.
  JOURNAL   Nat Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z.
  PUBMED    34183861
REFERENCE   2  (bases 1 to 11340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z."
COMMENT     Sequence Label: pCMV_POLD2-APOBEC1-UdgX-NG-nCas9-UdgX
FEATURES             Location/Qualifiers
     source          1..11340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             694..714
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2218..2901
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             3721..7821
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8575..8595
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8604..8621
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8644..8661)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8650..8874
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8945..8961)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8945..8961)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8958..8980)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8969..8985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8993..8999,9000..9017,9018..9023))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9038..9059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9176..9193)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9347..9935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9427..9446)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10106..10897,10898..10966))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10729..10748
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10967..11071)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11150..11169)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtcatgttt
      721 tctgagcagg ctgcccagag ggcccacact ctactgtccc caccatcagc caacaatgcc
      781 acctttgccc gggtgccagt ggcaacctac accaactcct cacaaccctt ccggctagga
      841 gagcgcagct ttagccggca gtatgcccac atttatgcca cccgcctcat ccaaatgaga
      901 cccttcctgg agaaccgggc ccagcagcac tggggcagtg gagtgggagt gaagaagctg
      961 tgtgaactgc agcctgagga gaagtgctgt gtggtgggca ctctgttcaa ggccatgccg
     1021 ctgcagccct ccatcctgcg ggaggtcagc gaggagcaca acctgctccc ccagcctcct
     1081 cggagtaaat acatacaccc agatgacgag ctggtcttgg aagatgaact gcagcgtatc
     1141 aaactaaaag gcaccattga cgtgtcaaag ctggttacgg ggactgtcct ggctgtgttt
     1201 ggctccgtga gagacgacgg gaagtttctg gtggaggact attgctttgc tgaccttgct
     1261 ccccagaagc ccgcaccccc acttgacaca gataggtttg tgctactggt gtccggcctg
     1321 ggcctgggtg gcggtggagg cgagagcctg ctgggcaccc agctgctggt ggatgtggtg
     1381 acggggcagc ttggggacga aggggagcag tgcagcgccg cccacgtctc ccgggttatc
     1441 ctcgctggca acctcctcag ccacagcacc cagagcaggg attctatcaa taaggccaaa
     1501 tacctcacca agaaaaccca ggcagccagc gtggaggctg ttaagatgct ggatgagatc
     1561 ctcctgcagc tgagcgcctc agtgcccgtg gacgtgatgc caggcgagtt tgatcccacc
     1621 aattacacgc tcccccagca gcccctccac ccctgcatgt tcccgctggc cactgcctac
     1681 tccacgctcc agctggtcac caacccctac caggccacca ttgatggagt cagatttttg
     1741 gggacatcag gacagaacgt gagtgacatt ttccgataca gcagcatgga ggatcacttg
     1801 gagatcctgg agtggaccct gcgggtccgt cacatcagcc ccacagcccc tgacactcta
     1861 ggttgttacc ccttctacaa aactgacccg ttcatcttcc cagagtgccc gcatgtctac
     1921 ttttgtggca acacccccag ctttggctcc aaaatcatcc gaggtcctga ggaccagaca
     1981 gtgctgttgg tgactgtccc tgacttcagt gccacgcaga ccgcctgcct tgtgaacctg
     2041 cgcagcctgg cctgccagcc catcagcttc tcgggcttcg gggcagagga cgatgacctg
     2101 ggaggcctgg ggctgggccc ctctggagga tctagcggag gctcctctgg cagcgagaca
     2161 ccaggaacaa gcgagtcagc aacaccagag agcagtggcg gcagcagcgg ggggtcatcc
     2221 tcagagactg ggcctgtcgc cgtcgatcca accctgcgcc gccggattga acctcacgag
     2281 tttgaagtgt tctttgaccc ccgggagctg agaaaggaga catgcctgct gtacgagatc
     2341 aactggggag gcaggcactc catctggagg cacacctctc agaacacaaa taagcacgtg
     2401 gaggtgaact tcatcgagaa gtttaccaca gagcggtact tctgccccaa taccagatgt
     2461 agcatcacat ggtttctgag ctggtcccct tgcggagagt gtagcagggc catcaccgag
     2521 ttcctgtcca gatatccaca cgtgacactg tttatctaca tcgccaggct gtatcaccac
     2581 gcagacccaa ggaataggca gggcctgcgc gatctgatca gctccggcgt gaccatccag
     2641 atcatgacag agcaggagtc cggctactgc tggcggaact tcgtgaatta ttctcctagc
     2701 aacgaggccc actggcctag gtacccacac ctgtgggtgc gcctgtacgt gctggagctg
     2761 tattgcatca tcctgggcct gcccccttgt ctgaatatcc tgcggagaaa gcagccccag
     2821 ctgaccttct ttacaatcgc cctgcagtct tgtcactatc agaggctgcc accccacatc
     2881 ctgtgggcca caggcctgaa gtctggagga tctagcggag gatcctctgg cagcgagaca
     2941 ccaggaacaa gcgagtcagc aacaccagag agcagtggcg gcagcagcgg cggcagcatg
     3001 gccggagcac aggattttgt cccacatact gccgatctgg ctgagctggc tgccgccgct
     3061 ggagagtgta gaggatgcgg gctgtaccgg gatgccacac aggccgtgtt cggagcaggc
     3121 ggcaggagcg cccgcatcat gatgatcggc gagcagccag gcgataagga ggacctggcc
     3181 ggcctgccat ttgtgggacc agcaggccgg ctgctggaca gagccctgga ggccgccgac
     3241 atcgataggg acgccctgta cgtgaccaac gccgtgaagc acttcaagtt tacacgcgcc
     3301 gcaggaggca agaggagaat ccacaagacc ccctctcgga cagaggtggt ggcctgcaga
     3361 ccttggctga tcgccgagat gaccagcgtg gagccagatg tggtggtgct gctgggagca
     3421 acagcagcaa aggccctgct gggcaatgac ttcagggtga cccagcacag gggagaggtg
     3481 ctgcacgtgg acgatgtgcc aggcgatcct gccctggtgg caaccgtgca ccctagctcc
     3541 ctgctgagag gcccaaagga ggagcgggaa tccgccttcg ctgggctggt ggacgacctg
     3601 agagtggctg ccgatgtgag acctagcggt ggtagttctg gcggtagctc aggatctgaa
     3661 actcccggca catctgaaag cgcgactccc gaatcatctg gtggatcttc aggtggaagt
     3721 gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc
     3781 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
     3841 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
     3901 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
     3961 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
     4021 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
     4081 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
     4141 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
     4201 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
     4261 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
     4321 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg
     4381 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg
     4441 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
     4501 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
     4561 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
     4621 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
     4681 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
     4741 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
     4801 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
     4861 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag
     4921 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
     4981 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
     5041 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
     5101 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg
     5161 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac
     5221 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat
     5281 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc
     5341 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
     5401 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
     5461 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
     5521 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
     5581 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
     5641 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
     5701 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
     5761 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc
     5821 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
     5881 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
     5941 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
     6001 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
     6061 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
     6121 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
     6181 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc
     6241 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac
     6301 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
     6361 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
     6421 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
     6481 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
     6541 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
     6601 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
     6661 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac
     6721 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
     6781 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
     6841 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct
     6901 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
     6961 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
     7021 acaggcggct tcagcaaaga gtctatcagg cccaagagga acagcgataa gctgatcgcc
     7081 agaaagaagg actgggaccc taagaagtac ggcggcttcg tcagccccac cgtggcctat
     7141 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
     7201 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
     7261 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
     7321 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccag attcctgcag
     7381 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
     7441 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
     7501 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
     7561 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
     7621 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
     7681 agggccttca agtactttga caccaccatc gaccggaagg tgtacaggag caccaaagag
     7741 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
     7801 ctgtctcagc tgggaggtga ctctggagga tctagcggag gatcctctgg cagcgagaca
     7861 ccaggaacaa gcgagtcagc aacaccagag agcagcggcg gcagcagcgg cggcagcatg
     7921 gccggagcac aggattttgt cccacatact gccgatctgg ctgagctggc tgccgccgct
     7981 ggagagtgta gaggatgcgg gctgtaccgg gatgccacac aggccgtgtt cggagcaggc
     8041 ggcaggagcg cccgcatcat gatgatcggc gagcagccag gcgataagga ggacctggcc
     8101 ggcctgccat ttgtgggacc agcaggccgg ctgctggaca gagccctgga ggccgccgac
     8161 atcgataggg acgccctgta cgtgaccaac gccgtgaagc acttcaagtt tacacgcgcc
     8221 gcaggaggca agaggagaat ccacaagacc ccctctcgga cagaggtggt ggcctgcaga
     8281 ccttggctga tcgccgagat gaccagcgtg gagccagatg tggtggtgct gctgggagca
     8341 acagcagcaa aggccctgct gggcaatgac ttcagggtga cccagcacag gggagaggtg
     8401 ctgcacgtgg acgatgtgcc aggcgatcct gccctggtgg caaccgtgca ccctagctcc
     8461 ctgctgagag gcccaaagga ggagcgggaa tccgccttcg ctgggctggt ggacgacctg
     8521 agagtggctg ccgatgtgag acctaaaaga accgccgacg gcagcgaatt cgagcccaag
     8581 aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa acccgctgat
     8641 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     8701 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     8761 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     8821 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     8881 aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc ttggcgtaat
     8941 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9001 gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa ctcacattaa
     9061 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     9121 gaatcggcca acgcgcggga agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9181 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9241 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9301 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9361 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9421 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9481 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9541 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9601 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9661 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9721 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9781 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9841 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9901 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9961 ggtctgacac tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10021 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10081 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    10141 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10201 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10261 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10321 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10381 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10441 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10501 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10561 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10621 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10681 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10741 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10801 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10861 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10921 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10981 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    11041 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    11101 tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct
    11161 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
    11221 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
    11281 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
//
