LOCUS       sequence-320245-        9849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian CG-to-GC base editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, 
            Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, 
            Adamson B, Liu DR
  TITLE     Efficient C*G-to-G*C base editors developed using CRISPRi screens, 
            target-library analysis, and machine learning.
  JOURNAL   Nat Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z.
  PUBMED    34183861
REFERENCE   2  (bases 1 to 9849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z."
COMMENT     Sequence Label: pCMV_UdgX-APOBEC1-UdgX-NG-nCas9
FEATURES             Location/Qualifiers
     source          1..9849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     246..265
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        246..264
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             314..334
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1058..1741
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             2561..6661
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6704..6724
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6733..6750
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6773..6790)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6779..7003
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7074..7090)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7074..7090)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7087..7109)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7098..7114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7122..7128,7129..7146,7147..7152))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7167..7188
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7305..7322)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7476..8064)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7556..7575)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8235..9026,9027..9095))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8858..8877
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9096..9200)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9279..9298)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9470..9849
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg
      241 gccgctaata cgactcacta tagggagagc cgccaccatg aaacggacag ccgacggaag
      301 cgagttcgag tcaccaaaga agaagcggaa agtcatggcc ggagcacagg attttgtccc
      361 acatactgcc gatctggctg agctggctgc cgccgctgga gagtgtagag gatgcgggct
      421 gtaccgggat gccacacagg ccgtgttcgg agcaggcggc aggagcgccc gcatcatgat
      481 gatcggcgag cagccaggcg ataaggagga cctggccggc ctgccatttg tgggaccagc
      541 aggccggctg ctggacagag ccctggaggc cgccgacatc gatagggacg ccctgtacgt
      601 gaccaacgcc gtgaagcact tcaagtttac acgcgccgca ggaggcaaga ggagaatcca
      661 caagaccccc tctcggacag aggtggtggc ctgcagacct tggctgatcg ccgagatgac
      721 cagcgtggag ccagatgtgg tggtgctgct gggagcaaca gcagcaaagg ccctgctggg
      781 caatgacttc agggtgaccc agcacagggg agaggtgctg cacgtggacg atgtgccagg
      841 cgatcctgcc ctggtggcaa ccgtgcaccc tagctccctg ctgagaggcc caaaggagga
      901 gcgggaatcc gccttcgctg ggctggtgga cgacctgaga gtggctgccg atgtgagacc
      961 ttctggagga tctagcggag gctcctctgg cagcgagaca ccaggaacaa gcgagtcagc
     1021 aacaccagag agcagtggcg gcagcagcgg ggggtcatcc tcagagactg ggcctgtcgc
     1081 cgtcgatcca accctgcgcc gccggattga acctcacgag tttgaagtgt tctttgaccc
     1141 ccgggagctg agaaaggaga catgcctgct gtacgagatc aactggggag gcaggcactc
     1201 catctggagg cacacctctc agaacacaaa taagcacgtg gaggtgaact tcatcgagaa
     1261 gtttaccaca gagcggtact tctgccccaa taccagatgt agcatcacat ggtttctgag
     1321 ctggtcccct tgcggagagt gtagcagggc catcaccgag ttcctgtcca gatatccaca
     1381 cgtgacactg tttatctaca tcgccaggct gtatcaccac gcagacccaa ggaataggca
     1441 gggcctgcgc gatctgatca gctccggcgt gaccatccag atcatgacag agcaggagtc
     1501 cggctactgc tggcggaact tcgtgaatta ttctcctagc aacgaggccc actggcctag
     1561 gtacccacac ctgtgggtgc gcctgtacgt gctggagctg tattgcatca tcctgggcct
     1621 gcccccttgt ctgaatatcc tgcggagaaa gcagccccag ctgaccttct ttacaatcgc
     1681 cctgcagtct tgtcactatc agaggctgcc accccacatc ctgtgggcca caggcctgaa
     1741 gtctggagga tctagcggag gatcctctgg cagcgagaca ccaggaacaa gcgagtcagc
     1801 aacaccagag agcagtggcg gcagcagcgg cggcagcatg gccggagcac aggattttgt
     1861 cccacatact gccgatctgg ctgagctggc tgccgccgct ggagagtgta gaggatgcgg
     1921 gctgtaccgg gatgccacac aggccgtgtt cggagcaggc ggcaggagcg cccgcatcat
     1981 gatgatcggc gagcagccag gcgataagga ggacctggcc ggcctgccat ttgtgggacc
     2041 agcaggccgg ctgctggaca gagccctgga ggccgccgac atcgataggg acgccctgta
     2101 cgtgaccaac gccgtgaagc acttcaagtt tacacgcgcc gcaggaggca agaggagaat
     2161 ccacaagacc ccctctcgga cagaggtggt ggcctgcaga ccttggctga tcgccgagat
     2221 gaccagcgtg gagccagatg tggtggtgct gctgggagca acagcagcaa aggccctgct
     2281 gggcaatgac ttcagggtga cccagcacag gggagaggtg ctgcacgtgg acgatgtgcc
     2341 aggcgatcct gccctggtgg caaccgtgca ccctagctcc ctgctgagag gcccaaagga
     2401 ggagcgggaa tccgccttcg ctgggctggt ggacgacctg agagtggctg ccgatgtgag
     2461 acctagcggt ggtagttctg gcggtagctc aggatctgaa actcccggca catctgaaag
     2521 cgcgactccc gaatcatctg gtggatcttc aggtggaagt gacaagaagt acagcatcgg
     2581 cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc
     2641 cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat
     2701 cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc
     2761 cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa
     2821 cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga
     2881 agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta
     2941 ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa
     3001 ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt
     3061 cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct
     3121 ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg gcgtggacgc
     3181 caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca
     3241 gctgcccggc gagaagaaga atggcctgtt cggaaacctg attgccctga gcctgggcct
     3301 gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc agctgagcaa
     3361 ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga
     3421 cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca tcctgagagt
     3481 gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat acgacgagca
     3541 ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg agaagtacaa
     3601 agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg gcggagccag
     3661 ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga
     3721 actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg
     3781 cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga
     3841 tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat
     3901 cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgaccagaaa
     3961 gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg gcgcttccgc
     4021 ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg agaaggtgct
     4081 gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga ccaaagtgaa
     4141 atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat
     4201 cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta
     4261 cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa
     4321 cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga
     4381 caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga
     4441 cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat
     4501 gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa
     4561 cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt
     4621 cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat
     4681 ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc
     4741 cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt
     4801 gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca
     4861 gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat
     4921 caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa
     4981 cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact
     5041 ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga gctttctgaa
     5101 ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg gcaagagcga
     5161 caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc agctgctgaa
     5221 cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct
     5281 gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac
     5341 aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa
     5401 gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa
     5461 ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta
     5521 cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt
     5581 cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga
     5641 aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac
     5701 cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga
     5761 aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag
     5821 catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga
     5881 gtctatcagg cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc
     5941 taagaagtac ggcggcttcg tcagccccac cgtggcctat tctgtgctgg tggtggccaa
     6001 agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat
     6061 catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa
     6121 agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa
     6181 cggccggaag agaatgctgg cctctgccag attcctgcag aagggaaacg aactggccct
     6241 gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc
     6301 ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga
     6361 gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga
     6421 caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa
     6481 tatcatccac ctgtttaccc tgaccaatct gggagcccct agggccttca agtactttga
     6541 caccaccatc gaccggaagg tgtacaggag caccaaagag gtgctggacg ccaccctgat
     6601 ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggtga
     6661 ctctggcggc tcaaaaagaa ccgccgacgg cagcgaattc gagcccaaga agaagaggaa
     6721 agtctaaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact
     6781 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     6841 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     6901 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     6961 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     7021 accagctggg gctcgatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     7081 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7141 ataaagtgta aagcctaggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7201 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7261 cgcgcgggaa gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7321 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7381 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7441 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7501 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7561 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7621 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7681 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7741 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7801 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7861 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7921 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7981 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8041 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacact
     8101 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8161 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8221 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8281 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8341 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8401 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8461 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8521 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8581 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8641 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8701 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8761 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8821 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8881 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8941 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9001 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9061 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9121 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9181 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     9241 ggagatcgat ctcccgatcc cctagggtcg actctcagta caatctgctc tgatgccgca
     9301 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     9361 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     9421 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     9481 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     9541 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     9601 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     9661 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     9721 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     9781 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     9841 attaccatg
//
