LOCUS       sequence-320246-       11106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian CG-to-GC base editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, 
            Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, 
            Adamson B, Liu DR
  TITLE     Efficient C*G-to-G*C base editors developed using CRISPRi screens, 
            target-library analysis, and machine learning.
  JOURNAL   Nat Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z.
  PUBMED    34183861
REFERENCE   2  (bases 1 to 11106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z."
COMMENT     Sequence Label: pCMV_UdgX-EE-UdgX-nCas9-RBMX
FEATURES             Location/Qualifiers
     source          1..11106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(501..524,525..527,528..4601)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             501..4601
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5901..5921
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5930..5947
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5970..5987)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5976..6200
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6271..6287)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6271..6287)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6284..6306)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6295..6311
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6319..6325,6326..6343,6344..6349))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6364..6385
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6502..6519)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6673..7261)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6753..6772)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7432..8223,8224..8292))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8055..8074
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8293..8397)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8476..8495)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8667..9046
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9047..9250
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9201..9221
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9292..9311
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9292..9310
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9360..9380
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagccctgga ggccgccgac atcgataggg acgccctgta cgtgaccaac gccgtgaagc
       61 acttcaagtt tacacgcgcc gcaggaggca agaggagaat ccacaagacc ccctctcgga
      121 cagaggtggt ggcctgcaga ccttggctga tcgccgagat gaccagcgtg gagccagatg
      181 tggtggtgct gctgggagca acagcagcaa aggccctgct gggcaatgac ttcagggtga
      241 cccagcacag gggagaggtg ctgcacgtgg acgatgtgcc aggcgatcct gccctggtgg
      301 caaccgtgca ccctagctcc ctgctgagag gcccaaagga ggagcgggaa tccgccttcg
      361 ctgggctggt ggacgacctg agagtggctg ccgatgtgag acctagcggt ggtagttctg
      421 gcggtagctc aggatctgaa actcccggca catctgaaag cgcgactccc gaatcatctg
      481 gtggatcttc aggtggaagt gacaagaagt acagcatcgg cctggccatc ggcaccaact
      541 ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc
      601 tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca
      661 gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac
      721 ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg
      781 acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc
      841 ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca
      901 tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct
      961 atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga
     1021 accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc
     1081 tgttcgagga aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca
     1141 gactgagcaa gagcagacgg ctggaaaatc tgatcgccca gctgcccggc gagaagaaga
     1201 atggcctgtt cggaaacctg attgccctga gcctgggcct gacccccaac ttcaagagca
     1261 acttcgacct ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc
     1321 tggacaacct gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga
     1381 acctgtccga cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg
     1441 cccccctgag cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc
     1501 tgaaagctct cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga
     1561 gcaagaacgg ctacgccggc tacattgacg gcggagccag ccaggaagag ttctacaagt
     1621 tcatcaagcc catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaca
     1681 gagaggacct gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc
     1741 acctgggaga gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg
     1801 acaaccggga aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc
     1861 tggccagggg aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc
     1921 cctggaactt cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc atcgagcgga
     1981 tgaccaactt cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt
     2041 acgagtactt caccgtgtat aacgagctga ccaaagtgaa atacgtgacc gagggaatga
     2101 gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga
     2161 ccaaccggaa agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct
     2221 tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc
     2281 acgatctgct gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca
     2341 ttctggaaga tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac
     2401 ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga
     2461 gatacaccgg ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt
     2521 ccggcaagac aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc
     2581 agctgatcca cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg
     2641 gccagggcga tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga
     2701 agggcatcct gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca
     2761 agcccgagaa catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga
     2821 agaacagccg cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga
     2881 tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact
     2941 acctgcagaa tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg
     3001 actacgatgt ggaccatatc gtgcctcaga gctttctgaa ggacgactcc atcgacaaca
     3061 aggtgctgac cagaagcgac aagaaccggg gcaagagcga caacgtgccc tccgaagagg
     3121 tcgtgaagaa gatgaagaac tactggcggc agctgctgaa cgccaagctg attacccaga
     3181 gaaagttcga caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg
     3241 gcttcatcaa gagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc
     3301 tggactcccg gatgaacact aagtacgacg agaatgacaa gctgatccgg gaagtgaaag
     3361 tgatcaccct gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag
     3421 tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa
     3481 ccgccctgat caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc gactacaagg
     3541 tgtacgacgt gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca
     3601 agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg
     3661 gcgagatccg gaagcggcct ctgatcgaga caaacggcga aaccggggag atcgtgtggg
     3721 ataagggccg ggattttgcc accgtgcgga aagtgctgag catgccccaa gtgaatatcg
     3781 tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga
     3841 acagcgataa gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg
     3901 acagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca
     3961 agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg
     4021 agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga
     4081 tcatcaagct gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg
     4141 cctctgccgg cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact
     4201 tcctgtacct ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga
     4261 aacagctgtt tgtggaacag cacaagcact acctggacga gatcatcgag cagatcagcg
     4321 agttctccaa gagagtgatc ctggccgacg ctaatctgga caaagtgctg tccgcctaca
     4381 acaagcaccg ggataagccc atcagagagc aggccgagaa tatcatccac ctgtttaccc
     4441 tgaccaatct gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga
     4501 ggtacaccag caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc
     4561 tgtacgagac acggatcgac ctgtctcagc tgggaggtga ctctggagga tctagcggag
     4621 gatcctctgg cagcgagaca ccaggaacaa gcgagtcagc aacaccagag agcagtggcg
     4681 gcagcagcgg cggcagcatg gttgaagcag atcgcccagg aaagctcttc attggtgggc
     4741 ttaatacgga aacaaatgag aaagctcttg aagcagtatt tggcaaatat ggacgaatag
     4801 tggaagtact cttgatgaaa gaccgtgaaa ccaacaaatc aagaggattt gcttttgtca
     4861 cctttgaaag cccagcagac gctaaggatg cagccagaga catgaatgga aagtcattag
     4921 atggaaaagc catcaaggtg gaacaagcca ccaaaccatc atttgaaagt ggtagacgtg
     4981 gaccgcctcc acctccaaga agtagaggcc ctccaagagg tcttagaggt ggaagaggag
     5041 gaagtggagg aaccagggga cctccctcac ggggaggaca catggatgac ggtggatatt
     5101 ccatgaattt taacatgagt tcttccaggg gaccactccc agtaaaaaga ggaccaccac
     5161 caagaagtgg gggtcctcct cctaagagat ctgcaccttc aggaccagtt cgcagtagca
     5221 gtggaatggg aggaagagct cctgtatcac gtggaagaga tagttatgga ggtccacctc
     5281 gaagggaacc gctgccctct cgtagagatg tttatttgtc cccaagagat gatgggtatt
     5341 ctactaaaga cagctattca agcagagatt acccaagttc tcgtgatact agagattatg
     5401 caccaccacc acgagattat acttaccgtg attatggtca ttccagttca cgtgatgact
     5461 atccatcaag aggatatagc gatagagatg gatatggtcg tgatcgtgac tattcagatc
     5521 atccaagtgg aggttcctac agagattcat atgagagtta tggtaactca cgtagtgctc
     5581 cacctacacg agggcccccg ccatcttatg gtggaagcag tcgctatgat gattacagca
     5641 gctcacgtga cggatatggt ggaagtcgag acagttactc aagcagccga agtgatctct
     5701 actcaagtgg tcgtgatcgg gttggcagac aagaaagagg gcttccccct tctatggaaa
     5761 gggggtaccc tcctccacgt gattcctaca gcagttcaag ccgcggagca ccaagaggtg
     5821 gtggccgtgg aggaagccga tctgatagag ggggaggcag aagcagatac aaaagaaccg
     5881 ccgacggcag cgaattcgag cccaagaaga agaggaaagt ctaaccggtc atcatcacca
     5941 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     6001 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     6061 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     6121 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     6181 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct cgataccgtc
     6241 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     6301 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctagggtgc
     6361 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6421 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcgggaagag gcggtttgcg
     6481 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6541 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6601 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6661 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6721 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6781 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6841 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6901 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6961 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7021 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7081 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7141 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7201 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7261 agaagatcct ttgatctttt ctacggggtc tgacactcag tggaacgaaa actcacgtta
     7321 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     7381 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     7441 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7501 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7561 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     7621 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     7681 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     7741 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7801 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7861 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7921 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7981 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8041 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8101 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8161 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8221 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     8281 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     8341 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     8401 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcgatctc ccgatcccct
     8461 agggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     8521 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     8581 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     8641 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     8701 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     8761 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     8821 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     8881 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     8941 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     9001 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     9061 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     9121 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     9181 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     9241 aagcagagct ggtttagtga accgtcagat ccgctagaga tccgcggccg ctaatacgac
     9301 tcactatagg gagagccgcc accatgaaac ggacagccga cggaagcgag ttcgagtcac
     9361 caaagaagaa gcggaaagtc atggccggag cacaggattt tgtcccacat actgccgatc
     9421 tggctgagct ggctgccgcc gctggagagt gtagaggatg cgggctgtac cgggatgcca
     9481 cacaggccgt gttcggagca ggcggcagga gcgcccgcat catgatgatc ggcgagcagc
     9541 caggcgataa ggaggacctg gccggcctgc catttgtggg accagcaggc cggctgctgg
     9601 acagagccct ggaggccgcc gacatcgata gggacgccct gtacgtgacc aacgccgtga
     9661 agcacttcaa gtttacacgc gccgcaggag gcaagaggag aatccacaag accccctctc
     9721 ggacagaggt ggtggcctgc agaccttggc tgatcgccga gatgaccagc gtggagccag
     9781 atgtggtggt gctgctggga gcaacagcag caaaggccct gctgggcaat gacttcaggg
     9841 tgacccagca caggggagag gtgctgcacg tggacgatgt gccaggcgat cctgccctgg
     9901 tggcaaccgt gcaccctagc tccctgctga gaggcccaaa ggaggagcgg gaatccgcct
     9961 tcgctgggct ggtggacgac ctgagagtgg ctgccgatgt gagaccttct ggaggatcta
    10021 gcggaggctc ctctggcagc gagacaccag gaacaagcga gtcagcaaca ccagagagca
    10081 gtggcggcag cagcgggggg tcatcctcag agactgggcc tgtcgccgtc gatccaaccc
    10141 tgcgccgccg gattgaacct cacgagtttg aagtgttctt tgacccccgg gagctgagaa
    10201 aggagacatg cctgctgtac gagatcaact ggggaggcag gcactccatc tggaggcaca
    10261 cctctcagaa cacaaataag cacgtggagg tgaacttcat cgagaagttt accacagagc
    10321 ggtacttctg ccccaatacc agatgtagca tcacatggtt tctgagctgg tccccttgcg
    10381 gagagtgtag cagggccatc accgagttcc tgtccagata tccacacgtg acactgttta
    10441 tctacatcgc caggctgtat caccacgcag acccagagaa taggcagggc ctggaggatc
    10501 tgatcagctc cggcgtgacc atccagatca tgacagagca ggagtccggc tactgctggc
    10561 ggaacttcgt gaattattct cctagcaacg aggcccactg gcctaggtac ccacacctgt
    10621 gggtgcgcct gtacgtgctg gagctgtatt gcatcatcct gggcctgccc ccttgtctga
    10681 atatcctgcg gagaaagcag ccccagctga ccttctttac aatcgccctg cagtcttgtc
    10741 actatcagag gctgccaccc cacatcctgt gggccacagg cctgaagtct ggaggatcta
    10801 gcggaggatc ctctggcagc gagacaccag gaacaagcga gtcagcaaca ccagagagca
    10861 gtggcggcag cagcggcggc agcatggccg gagcacagga ttttgtccca catactgccg
    10921 atctggctga gctggctgcc gccgctggag agtgtagagg atgcgggctg taccgggatg
    10981 ccacacaggc cgtgttcgga gcaggcggca ggagcgcccg catcatgatg atcggcgagc
    11041 agccaggcga taaggaggac ctggccggcc tgccatttgt gggaccagca ggccggctgc
    11101 tggaca
//
