LOCUS       sequence-320253-       12119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12119)
  AUTHORS   Verma P, Zhou Y, Cao Z, Deraska PV, Deb M, Arai E, Li W, Shao Y, 
            Puentes L, Li Y, Patankar S, Mach RH, Faryabi RB, Shi J, Greenberg 
            RA
  TITLE     ALC1 links chromatin accessibility to PARP inhibitor response in 
            homologous recombination-deficient cells.
  JOURNAL   Nat Cell Biol. 2021 Jan 18. pii: 10.1038/s41556-020-00624-3. doi: 
            10.1038/s41556-020-00624-3.
  PUBMED    33462394
REFERENCE   2  (bases 1 to 12119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2021 Jan 18. pii: 10.1038/s41556-020-00624-3. doi: 
            10.1038/s41556-020-00624-3."
COMMENT     Sequence Label: SaLgCP
FEATURES             Location/Qualifiers
     source          1..12119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_RNA        complement(1372..1447)
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     promoter        complement(3329..3577)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(3387..3406)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(3557..3577)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    3584..3701
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3770..3981
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      4001..4010
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4010..4033
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4040..4060
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4085..7240
                     /codon_start=1
                     /product="Cas9 endonuclease from the Staphylococcus aureus 
                     Type II CRISPR/Cas system"
                     /label=SaCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="KRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEG
                     RRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEF
                     SAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEV
                     RGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGW
                     KDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQ
                     IIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEI
                     IENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAI
                     NLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIK
                     VINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAK
                     YLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVKQ
                     EENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFS
                     VQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERN
                     KGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIF
                     ITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKDN
                     DKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKD
                     NGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNL
                     DVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLN
                     RIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQI
                     IKKG"
     CDS             7241..7288
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7304..7360
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7361..7960
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7361..7380)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7857..7877
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7976..8564
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8029..8049)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8447..8458)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8636..8869
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8947..9068
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8984..9003)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9038..9057
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9108..9243
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9263..9282)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9264..9282)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9292..9309)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9292..9308)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9301..9323)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9450..9905
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9537..9556)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9747..9768
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9931..10035
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10036..10104,10105..10896)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10254..10273)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11067..11655
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11556..11575
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11809..11826
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11943..11964
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11979..11984,11985..12002,12003..12009)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12017..12033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12022..12044
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12041..12057
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12041..12057
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12076..12096
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12078..12096
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcgc catttgtctc aagatctaga attcaaaaaa atctcgccaa
     1381 caagttgacg agataaacac ggcattttgc cttgtttaag tagattctgt ttccagagta
     1441 cttaaacaga gacgtacaaa aaagagcaag aagctaaaaa agatttaaaa attattttta
     1501 gcgcagttaa tggaacagga actaaattta ccccaaaaat attacgtgaa tcaggatata
     1561 acgttattga ggttgaagag catgcatttg aagatgaaac atttaaaaat gttgtaaatc
     1621 caaatccaga atttgatcct gcatgaaaaa taccgcttga atatggtatt aaacatgatg
     1681 cagatattat tattatgaat gacccagatg ctgacagatt tggaatggca ataaaacatg
     1741 atggtcattt tgtaagatta gatggaaatc aaacaggacc aattttaatt gattgaaaat
     1801 tatcaaatct aaaacgctta aatagcattc caaaaaatcc ggctctatat tcaagttttg
     1861 taacaagtga tttgggtgat agaatcgctc atgaaaaata tggagttaat attgtaaaaa
     1921 ctttaactgg atttaaatga atgggtagag aaattgctaa agaagaagat aacggattaa
     1981 attttgtttt tgcttatgaa gaaagttatg gatatgtaat tgatgactca gctagagata
     2041 aagatggaat acaagcttct atattaatag cagaggctgc ttgattttat aaaaaacaaa
     2101 ataaaacatt agtagactat ttagaagatt tatttaaaga aatgggtgca tattacactt
     2161 tcactttaaa cttgaatttt aaaccagaag aaaagaaatt aaaaattgaa ccattaatga
     2221 aatcattgag agcaacaccc ttaactcaaa ttgctggact taaagttgtt aatgttgaag
     2281 actacatcga tggaatgtat aatatgccag gacaagactt actaaaattt tatttagaag
     2341 ataagtcatg atttgctgtt cgcccaagtg gaactgaacc taaactaaaa atttatttta
     2401 taggtgttgg tgaatctgtt caaaacgcta aagttaaagt agacgaaatt attaaagaat
     2461 taaaattaaa aatgaatata taggagaaaa aatgaaacta aacaaatata tagatcacac
     2521 attattaaaa caagatgcta cgaaagctga aattaaacaa ttatgtgatg aagcaattga
     2581 atttgatttt gcaacagttt gtgttaattc atattgaaca agctattgta aagaattatt
     2641 aaaaggcaca aatgtaggaa taacaaatgt tgtaggtttt cctctaggtg catgcacaac
     2701 agctacaaaa gcattcgaag tttctgaagc aattaaagat ggtgcaacag aaattgatat
     2761 ggtattaaat attggtgcat taaaagacaa aaattatgaa ttagttttag aagacatgaa
     2821 agctgtaaaa aaagcagctg gatcacatgt tgttaaatgt attatggaaa attgtttatt
     2881 aacaaaagaa gaaatcatga aagcttgtga aatagctgtt gaagctggat tagaatttgt
     2941 taaaacatca acaggatttt caaaatcagg tgcaacattt gaagatgtta aactaatgaa
     3001 gtcagttgtt aaagacaatg ctttagttaa agcagctggt ggagttagaa catttgaaga
     3061 tgctcaaaaa atgattgaag caggagctga ccgcttagga acaagtggtg gagtagctat
     3121 tattaaaggt gaagaaaaca acgcgagtta ctaaaactag cgttttttta ttttgctcat
     3181 ttttattaaa agtttgcaaa aaggaacata aaaattctaa ttattgatac taaagttatt
     3241 aaaaagaaga ttttggttga ttttataaag gtcatagaat ataatatttt agcatgtgta
     3301 ttttgtgtgc tcatttacaa ccgtctccgg tgtttcgtcc tttccacaag atatataaag
     3361 ccaagaaatc gaaatacttt caagttacgg taagcatatg atagtccatt ttaaaacata
     3421 attttaaaac tgcaaactac ccaagaaatt attactttct acgtcacgta ttttgtacta
     3481 atatctttgt gtttacagtc aaattaattc taattatctc tctaacagcc ttgtatcgta
     3541 tatgcaaata tgaaggaatc atgggaaata ggccctccca caattttaaa agaaaagggg
     3601 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     3661 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     3721 gcagagatcc actttggcgc cggctcgagt ggctccggtg cccgtcagtg ggcagagcgc
     3781 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
     3841 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     3901 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     3961 gggtttgccg ccagaacaca ggtgtcgtga cgcgggatcc gccaccatgg attacaaaga
     4021 cgatgacgat aagatggccc caaagaagaa gcggaaggtc ggtatccacg gagtcccagc
     4081 agccaagcgg aactacatcc tgggcctgga catcggcatc accagcgtgg gctacggcat
     4141 catcgactac gagacacggg acgtgatcga tgccggcgtg cggctgttca aagaggccaa
     4201 cgtggaaaac aacgagggca ggcggagcaa gagaggcgcc agaaggctga agcggcggag
     4261 gcggcataga atccagagag tgaagaagct gctgttcgac tacaacctgc tgaccgacca
     4321 cagcgagctg agcggcatca acccctacga ggccagagtg aagggcctga gccagaagct
     4381 gagcgaggaa gagttctctg ccgccctgct gcacctggcc aagagaagag gcgtgcacaa
     4441 cgtgaacgag gtggaagagg acaccggcaa cgagctgtcc accaaagagc agatcagccg
     4501 gaacagcaag gccctggaag agaaatacgt ggccgaactg cagctggaac ggctgaagaa
     4561 agacggcgaa gtgcggggca gcatcaacag attcaagacc agcgactacg tgaaagaagc
     4621 caaacagctg ctgaaggtgc agaaggccta ccaccagctg gaccagagct tcatcgacac
     4681 ctacatcgac ctgctggaaa cccggcggac ctactatgag ggacctggcg agggcagccc
     4741 cttcggctgg aaggacatca aagaatggta cgagatgctg atgggccact gcacctactt
     4801 ccccgaggaa ctgcggagcg tgaagtacgc ctacaacgcc gacctgtaca acgccctgaa
     4861 cgacctgaac aatctcgtga tcaccaggga cgagaacgag aagctggaat attacgagaa
     4921 gttccagatc atcgagaacg tgttcaagca gaagaagaag cccaccctga agcagatcgc
     4981 caaagaaatc ctcgtgaacg aagaggatat taagggctac agagtgacca gcaccggcaa
     5041 gcccgagttc accaacctga aggtgtacca cgacatcaag gacattaccg cccggaaaga
     5101 gattattgag aacgccgagc tgctggatca gattgccaag atcctgacca tctaccagag
     5161 cagcgaggac atccaggaag aactgaccaa tctgaactcc gagctgaccc aggaagagat
     5221 cgagcagatc tctaatctga agggctatac cggcacccac aacctgagcc tgaaggccat
     5281 caacctgatc ctggacgagc tgtggcacac caacgacaac cagatcgcta tcttcaaccg
     5341 gctgaagctg gtgcccaaga aggtggacct gtcccagcag aaagagatcc ccaccaccct
     5401 ggtggacgac ttcatcctga gccccgtcgt gaagagaagc ttcatccaga gcatcaaagt
     5461 gatcaacgcc atcatcaaga agtacggcct gcccaacgac atcattatcg agctggcccg
     5521 cgagaagaac tccaaggacg cccagaaaat gatcaacgag atgcagaagc ggaaccggca
     5581 gaccaacgag cggatcgagg aaatcatccg gaccaccggc aaagagaacg ccaagtacct
     5641 gatcgagaag atcaagctgc acgacatgca ggaaggcaag tgcctgtaca gcctggaagc
     5701 catccctctg gaagatctgc tgaacaaccc cttcaactat gaggtggacc acatcatccc
     5761 cagaagcgtg tccttcgaca acagcttcaa caacaaggtg ctcgtgaagc aggaagaaaa
     5821 cagcaagaag ggcaaccgga ccccattcca gtacctgagc agcagcgaca gcaagatcag
     5881 ctacgaaacc ttcaagaagc acatcctgaa tctggccaag ggcaagggca gaatcagcaa
     5941 gaccaagaaa gagtatctgc tggaagaacg ggacatcaac aggttctccg tgcagaaaga
     6001 cttcatcaac cggaacctgg tggataccag atacgccacc agaggcctga tgaacctgct
     6061 gcggagctac ttcagagtga acaacctgga cgtgaaagtg aagtccatca atggcggctt
     6121 caccagcttt ctgcggcgga agtggaagtt taagaaagag cggaacaagg ggtacaagca
     6181 ccacgccgag gacgccctga tcattgccaa cgccgatttc atcttcaaag agtggaagaa
     6241 actggacaag gccaaaaaag tgatggaaaa ccagatgttc gaggaaaagc aggccgagag
     6301 catgcccgag atcgaaaccg agcaggagta caaagagatc ttcatcaccc cccaccagat
     6361 caagcacatt aaggacttca aggactacaa gtacagccac cgggtggaca agaagcctaa
     6421 tagagagctg attaacgaca ccctgtactc cacccggaag gacgacaagg gcaacaccct
     6481 gatcgtgaac aatctgaacg gcctgtacga caaggacaat gacaagctga aaaagctgat
     6541 caacaagagc cccgaaaagc tgctgatgta ccaccacgac ccccagacct accagaaact
     6601 gaagctgatt atggaacagt acggcgacga gaagaatccc ctgtacaagt actacgagga
     6661 aaccgggaac tacctgacca agtactccaa aaaggacaac ggccccgtga tcaagaagat
     6721 taagtattac ggcaacaaac tgaacgccca tctggacatc accgacgact accccaacag
     6781 cagaaacaag gtcgtgaagc tgtccctgaa gccctacaga ttcgacgtgt acctggacaa
     6841 tggcgtgtac aagttcgtga ccgtgaagaa tctggatgtg atcaaaaaag aaaactacta
     6901 cgaagtgaat agcaagtgct atgaggaagc taagaagctg aagaagatca gcaaccaggc
     6961 cgagtttatc gcctccttct acaacaacga tctgatcaag atcaacggcg agctgtatag
     7021 agtgatcggc gtgaacaacg acctgctgaa ccggatcgaa gtgaacatga tcgacatcac
     7081 ctaccgcgag tacctggaaa acatgaacga caagaggccc cccaggatca ttaagacaat
     7141 cgcctccaag acccagagca ttaagaagta cagcacagac attctgggca acctgtatga
     7201 agtgaaatct aagaagcacc ctcagatcat caaaaagggc aagcgtcctg ctgctactaa
     7261 gaaagctggt caagctaaga aaaagaaagc tagcggcagc ggcgccacca acttcagcct
     7321 gctgaagcag gccggcgacg tggaggagaa ccccggcccc atgaccgagt acaagcccac
     7381 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     7441 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     7501 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     7561 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     7621 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     7681 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     7741 cgtcggagtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     7801 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     7861 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     7921 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga acgcgttaag tcgacaatca
     7981 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     8041 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     8101 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     8161 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     8221 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     8281 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     8341 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     8401 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     8461 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     8521 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca
     8581 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     8641 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     8701 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8761 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8821 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt
     8881 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     8941 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     9001 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9061 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     9121 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     9181 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     9241 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     9301 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     9361 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     9421 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     9481 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     9541 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     9601 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     9661 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     9721 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     9781 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     9841 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     9901 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     9961 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
    10021 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
    10081 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
    10141 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
    10201 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
    10261 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
    10321 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
    10381 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
    10441 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
    10501 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
    10561 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
    10621 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
    10681 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
    10741 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
    10801 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
    10861 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
    10921 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    10981 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    11041 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    11101 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    11161 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    11221 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    11281 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    11341 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    11401 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    11461 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    11521 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    11581 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    11641 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    11701 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    11761 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    11821 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
    11881 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    11941 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    12001 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
    12061 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagc
//
