LOCUS       sequence-320291-        5855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split GFP reporter expressing GFP(beta1-10) following C>U editing of
            the target site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Huang X, Lv J, Li Y, Mao S, Li Z, Jing Z, Sun Y, Zhang X, Shen S, 
            Wang X, Di M, Ge J, Huang X, Zuo E, Chi T
  TITLE     Programmable C-to-U RNA editing using the human APOBEC3A deaminase.
  JOURNAL   EMBO J. 2020 Nov 16;39(22):e104741. doi: 10.15252/embj.2020104741. 
            Epub 2020 Oct 15.
  PUBMED    33058229
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2020-11-16"; volume: "39(22):e104741. doi"; pages: " 
            10.15252/embj.2020104741. Epub 2020 Oct 15"
COMMENT     Sequence Label: TC1541A
FEATURES             Location/Qualifiers
     source          1..5855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(34..825,826..894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     657..676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(895..999)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1064..1367
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1368..1571
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1522..1542
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1602..1611
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3659..3679
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    4073..4661
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4126..4146)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4544..4555)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4664..4680)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4665..4681)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4682..4699)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4688..4895
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4959..4976)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5130..5718)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5210..5229)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
       61 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
      121 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
      181 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
      241 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
      301 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
      361 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
      421 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
      481 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
      541 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
      601 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
      661 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
      721 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
      781 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      841 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
      901 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
      961 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1021 acctgacgtc taagaaacca ttattatcat gacattaacc tatcgttaca taacttacgg
     1081 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1141 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1201 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1261 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1321 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1381 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1441 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     1501 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1561 taagcagagc tggtttagtg aaccgtcaga tcgaattcgc cgccaccatg gaagtaagtg
     1621 ctcttgaaaa agaggtgtct gcccttgaaa aggaagtctc agcactggaa aaggaggtat
     1681 ccgcacttga gaaggaggta agtgccctgg agaagggcgg cagcggcggc atgtccaagg
     1741 gagaggagct gttcaccgga gtggtgccaa tcctggtgga gctggacggc gatgtgaacg
     1801 gccacaagtt ttctgtgcgc ggagagggag agggcgacgc aaccatcggc aagctgacac
     1861 tgaagttcat ctgcaccaca ggcaagctgc ccgtgccttg gccaaccctg gtgaccacac
     1921 tgacatacgg cgtgcagtgt ttctctaggt atccagacca catgaagaga cacgatttct
     1981 ttaagagcgc catgccagag ggatacgtgc aggagcggac catctccttc aaggacgatg
     2041 gcaagtataa gacccgcgcc gtggtgaagt ttgagggcga tacactggtg aacaggatcg
     2101 agctgaaggg caccgacttc aaggaggatg gcaatatcct gggccacaag ctggagtaca
     2161 actttaatag ccacaacgtg tatatcacag ccgacaagca gaagaacggc atcaaggcca
     2221 acttcaccgt tcgccacaat gtggaggacg gctccgtgca ggtactgggt ccattaactt
     2281 ctcccggagg cgcgaccgtc aggctcatac tccaacgggt cgtgccagcc ataaaatcac
     2341 cccaattatc catcttggct attgggaggc tgacctgaaa tatttacaag ccagacattc
     2401 ttagtgtttt atggagcagt gggtgatctg ctggtcccca gggtggcgtc ttgggagatg
     2461 acctctgagg ttccccctcc gctcccccca ccccccacgg atttctgcta agaaggctgg
     2521 aaggaaatgc ggggcgcaga ccaaaagatt aatattgatg ttcccttcta aagagaaaca
     2581 aatgttttgg taactttcac tgaagggaga tccaagcggt accagtggag agttggctga
     2641 ggagctaaag gggtgaaaaa ttcgggccgg gcatgatttt cccaagagtt ccaggatccg
     2701 accagcatcg tggcagcttg ggagaggtcc aggggaaggg aggaccagcg ctaggcctgc
     2761 gcgcgggaag agtttgtttt taattaaaag gaaactccct tttttccccc tctgtaagaa
     2821 cgatatattt cagagcatgg atttgtttac cagactggaa tctggaaaaa ataaatcggg
     2881 agaatgcatt tttcttttga tttttacaat agggaatgat tctgcagttg aaacttaaac
     2941 cctttctatc ctcaaaacaa gttgatttgc aggcgacagg ccctctgtgg ttcttcttcc
     3001 caggcctttg cgggggacca gtgtcttcag gtggggaggg gggacggtga agggtgaagc
     3061 tggtggaaga agtctcttta gagtgttcag agacaagtag ccaaccgatc ctcggagagg
     3121 ggcaggagga acctttctcg gttctgaatt ggaggtgcga gatcttcagg tttcatctcc
     3181 agtctaagta attaaaaccc cacgaaagtg tgagggtttt aacgtaagat ggagacagag
     3241 aaaagcagtt taagaccgag agaaggcaga agccccggat gctcacagat gcagaacaca
     3301 gcactgagcc ctgctcaagg gtagggagag ggttgagtac gttagctggg cctttgaggt
     3361 gtcttttggg atctttcccc ctccttccat gcttctcaat cgtgtgcgct cctcttttct
     3421 ccagctggcc gatcactatc aacaaaacac acctatcggc gacggacccg tgctgctgcc
     3481 tgataatcac tatctgtcca cccaaaccgt gctgtctaag gacccaaacg agaaggcggc
     3541 cgctacttgc caccgacgac caaaatatcg agcagacacc gtgaggataa attattcgaa
     3601 tattacgacc aacggagcgg ccgctaagcg caccgccgat ggctccgagt tcgaatcccc
     3661 taaaaagaaa agaaaggtgg gaggaggatc tggcgggaag gttagcgcac tgaaggaaaa
     3721 agtttcagca ctgaaagaga aggtgagtgc cctcaaagag aaagtctccg ctctcaagga
     3781 aaaagtctca gcccttaaag agagaaaggt gggaggagga tccggcggga actcctctcc
     3841 accagcagtc accctgacgc acccaatcac caaaatcgat accaaataca tcatgacatg
     3901 catgtcggcc gacctggagg tcgtcacgag cacctgggtg ctcgttggcg gcgtcctggc
     3961 tgctctggcc gcgtattgcc tgtcaacagg ctgcgtggtc atagtgggca ggatcgtctt
     4021 gtccgggaag ccggcaatta tacctgacag ggaggttctc taggctagct agaatcaacc
     4081 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4141 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4201 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4261 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4321 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4381 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4441 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     4501 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4561 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4621 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     4681 acctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     4741 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     4801 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     4861 gaggattggg aagacaatag caggcatgct ggggagcatt aatgaatcgg ccaacgcgcg
     4921 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     4981 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5041 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     5101 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5161 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5221 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5281 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     5341 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5401 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5461 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5521 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5581 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5641 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5701 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5761 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5821 atccttttaa attaaaaatg aagttttaaa tcaat
//
