LOCUS       sequence-320293-        9627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Li L, Dutra A, Pak E, Labrie JE, Gerstein RM, Pandolfi PP, Recht LD,
            Ross AH
  TITLE     EGFRvIII expression and PTEN loss synergistically induce chromosomal
            instability and glial tumors.
  JOURNAL   Neuro Oncol. 2009 Feb . 11(1):9-21.
  PUBMED    18812521
REFERENCE   2  (bases 1 to 9627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuro 
            Oncol. 2009 Feb . 11(1):9-21."
COMMENT     Sequence Label: MSCV-XZ066-EGFRvIII
FEATURES             Location/Qualifiers
     source          1..9627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(4599..4615)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4600..4616)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    4628..5197
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4647..5199
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4791..4808)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5018..5037
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5211..5213,5214..5216,5217..5930)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5256..5277)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5517..5536)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5864..5885
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6022..6536
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6698..6714
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6722..6728,6729..6746,6747..6752))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6767..6788
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6905..6922)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7076..7664)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7156..7175)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7835..8626,8627..8695))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8696..8800)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8868..8886
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8924..8946)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9046..9065
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9259..9281
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9409..9428
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatctctc gagcggccgc tctagagagc
     1441 ttatcgattc tagccgagtc cccgcctcgc cgccaacgcc acaaccaccg cgcacggccc
     1501 cctgactccg tccagtattg atcgggagag ccggagcgag ctcttcgggg agcagcgatg
     1561 cgaccctccg ggacggccgg ggcagcgttc ctggcgctgc tggctgcgct ctgcccggcg
     1621 agtcgggctc tggaggaaaa gaaaggtaat tatgtggtga cagatcacgg ctcgtgcgtc
     1681 cgagcctgtg gggccgacag ctatgagatg gaggaagacg gcgtccgcaa gtgtaagaag
     1741 tgcgaagggc cttgccgcaa agtgtgtaac ggaataggta ttggtgaatt taaagactca
     1801 ctctccataa atgctacgaa tattaaacac ttcaaaaact gcacctccat cagtggcgat
     1861 ctccacatcc tgccggtggc atttaggggt gactccttca cacatactcc tcctctggat
     1921 ccacaggaac tggatattct gaaaaccgta aaggaaatca cagggttttt gctgattcag
     1981 gcttggcctg aaaacaggac ggacctccat gcctttgaga acctagaaat catacgcggc
     2041 aggaccaagc aacatggtca gttttctctt gcagtcgtca gcctgaacat aacatccttg
     2101 ggattacgct ccctcaagga gataagtgat ggagatgtga taatttcagg aaacaaaaat
     2161 ttgtgctatg caaatacaat aaactggaaa aaactgtttg ggacctccgg tcagaaaacc
     2221 aaaattataa gcaacagagg tgaaaacagc tgcaaggcca caggccaggt ctgccatgcc
     2281 ttgtgctccc ccgagggctg ctggggcccg gagcccaggg actgcgtctc ttgccggaat
     2341 gtcagccgag gcagggaatg cgtggacaag tgcaaccttc tggagggtga gccaagggag
     2401 tttgtggaga actctgagtg catacagtgc cacccagagt gcctgcctca ggccatgaac
     2461 atcacctgca caggacgggg accagacaac tatatccagt gtgcccacta cattgacggc
     2521 ccccactgcg tcaagacctg cccggcagga gtcatgggag aaaacaacac cctggtctgg
     2581 aagtacgcag acgccggcca tgtgtgccac ctgtgccatc caaactgcac ctacggatgc
     2641 actgggccag gtcttgaagg ctgtccaacg aatgggccta agatcccgtc catcgccact
     2701 gggatggtgg gggccctcct cttgctgctg gtggtggccc tggggatcgg cctcttcatg
     2761 cgaaggcgcc acatcgttcg gaagcgcacg ctgcggaggc tgctgcagga gagggagctt
     2821 gtggagcctc ttacacccag tggagaagct cccaaccaag ctctcttgag gatcttgaag
     2881 gaaactgaat tcaaaaagat caaagtgctg ggctccggtg cgttcggcac ggtgtataag
     2941 ggactctgga tcccagaagg tgagaaagtt aaaattcccg tcgctatcaa ggaattaaga
     3001 gaagcaacat ctccgaaagc caacaaggaa atcctcgatg aagcctacgt gatggccagc
     3061 gtggacaacc cccacgtgtg ccgcctgctg ggcatctgcc tcacctccac cgtgcaactc
     3121 atcacgcagc tcatgccctt cggctgcctc ctggactatg tccgggaaca caaagacaat
     3181 attggctccc agtacctgct caactggtgt gtgcagatcg caaagggcat gaactacttg
     3241 gaggaccgtc gcttggtgca ccgcgacctg gcagccagga acgtactggt gaaaacaccg
     3301 cagcatgtca agatcacaga ttttgggctg gccaaactgc tgggtgcgga agagaaagaa
     3361 taccatgcag aaggaggcaa agtgcctatc aagtggatgg cattggaatc aattttacac
     3421 agaatctata cccaccagag tgatgtctgg agctacgggg tgaccgtttg ggagttgatg
     3481 acctttggat ccaagccata tgacggaatc cctgccagcg agatctcctc catcctggag
     3541 aaaggagaac gcctccctca gccacccata tgtaccatcg atgtctacat gatcatggtc
     3601 aagtgctgga tgatagacgc agatagtcgc ccaaagttcc gtgagttgat catcgaattc
     3661 tccaaaatgg cccgagaccc ccagcgctac cttgtcattc agggggatga aagaatgcat
     3721 ttgccaagtc ctacagactc caacttctac cgtgccctga tggatgaaga agacatggac
     3781 gacgtggtgg atgccgacga gtacctcatc ccacagcagg gcttcttcag cagcccctcc
     3841 acgtcacgga ctcccctcct gagctctctg agtgcaacca gcaacaattc caccgtggct
     3901 tgcattgata gaaatgggct gcaaagctgt cccatcaagg aagacagctt cttgcagcga
     3961 tacagctcag accccacagg cgccttgact gaggacagca tagacgacac cttcctccca
     4021 gtgcctgaat acataaacca gtccgttccc aaaaggcccg ctggctctgt gcagaatcct
     4081 gtctatcaca atcagcctct gaaccccgcg cccagcagag acccacacta ccaggacccc
     4141 cacagcactg cagtgggcaa ccccgagtat ctcaacactg tccagcccac ctgtgtcaac
     4201 agcacattcg acagccctgc ccactgggcc cagaaaggca gccaccaaat tagcctggac
     4261 aaccctgact accagcagga cttctttccc aaggaagcca agccaaatgg catctttaag
     4321 ggctccacag ctgaaaatgc agaataccta agggtcgcgc cacaaagcag tgaatttatt
     4381 ggagcatgac cacggaggat agtatgagcc ctaaaaatcc agactctttc gatacccagg
     4441 accaagccac agcaggtcct ccatcccaac agccatgccc gcattagctc ttagacccac
     4501 agactggttt tgcaacgttt acaccgacta gccaggaagt atccttcgcg ggacgtcctt
     4561 tgtttacgtc ccgtcggcgc tgaatccccc aagcttatcg ataccgtcga cctcgaggtt
     4621 aacgaattcc gccccccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     4681 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     4741 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     4801 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     4861 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     4921 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     4981 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     5041 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     5101 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     5161 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc atggtgagca
     5221 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     5281 acggccacaa gttcagcgtg tctggcgagg gcgagggcga tgccacctac ggcaagctga
     5341 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     5401 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     5461 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     5521 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     5581 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     5641 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     5701 cgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     5761 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     5821 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     5881 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa tgaattaatt
     5941 aagaattgcg gccgcgtcga cctgcagcca agcttatcga taaaataaaa gattttattt
     6001 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     6061 gtaacgccat tttgcaaggc atggaaaata cataactgag aatagagaag ttcagatcaa
     6121 ggttaggaac agagagacag cagaatatgg gccaaacagg atatctgtgg taagcagttc
     6181 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt
     6241 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     6301 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     6361 tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc
     6421 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
     6481 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatgggt
     6541 aacagtttct tgaagttgga gaacaacatt ctgagggtag gagtcgaata ttaagtaatc
     6601 ctgactcaat tagccactgt tttgaatcca catactccaa tactcctgaa atagttcatt
     6661 atggacagcg cagaaagagc tggggagaat tgtgaaattg ttatccgctc acaattccac
     6721 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     6781 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     6841 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     6901 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6961 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7021 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7081 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7141 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7201 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7261 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7321 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7381 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7441 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7501 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7561 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7621 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7681 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7741 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7801 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7861 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7921 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7981 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8041 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8101 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8161 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8221 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8281 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8341 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8401 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8461 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8521 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8581 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8641 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8701 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8761 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8821 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     8881 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     8941 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     9001 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     9061 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     9121 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     9181 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     9241 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggcgcaa ggaatggtgc
     9301 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
     9361 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
     9421 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
     9481 gtagaggcga ttagtccaat ttgttaaaga caggatatca gtggtccagg ctctagtttt
     9541 gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa aataaaagat
     9601 tttatttagt ctccagaaaa agggggg
//
