LOCUS       sequence-320295-        8838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C to T DNA base editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Lee S, Ding N, Sun Y, Yuan T, Li J, Yuan Q, Liu L, Yang J, Wang Q, 
            Kolomeisky AB, Hilton IB, Zuo E, Gao X
  TITLE     Single C-to-T substitution using engineered APOBEC3G-nCas9 base 
            editors with minimum genome- and transcriptome-wide off-target 
            effects.
  JOURNAL   Sci Adv. 2020 Jul 15;6(29):eaba1773. doi: 10.1126/sciadv.aba1773. 
            eCollection 2020 Jul.
  PUBMED    32832622
REFERENCE   2  (bases 1 to 8838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-15"; volume: "6(29):eaba1773. doi"; pages: " 
            10.1126/sciadv.aba1773. eCollection 2020 Jul"
COMMENT     Sequence Label: A3G-BE4.4
FEATURES             Location/Qualifiers
     source          1..8838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        55..258
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     209..229
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     300..319
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        300..318
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             368..388
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1046..1069,1070..1072,1073..5146)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1046..5146
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5177..5425
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5456..5704
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5747..5767
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5776..5793
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5816..5833)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5822..6046
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6117..6133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6117..6133)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6130..6152)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6141..6157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6165..6171,6172..6189,6190..6195))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6210..6231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6348..6365)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6519..7107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6599..6618)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7278..8069,8070..8138))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7901..7920
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8139..8243)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8322..8341)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8513..8838,1..54)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
       61 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      121 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      181 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      241 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagagatc cgcggccgct
      301 aatacgactc actataggga gagccgccac catgaaacgg acagccgacg gaagcgagtt
      361 cgagtcacca aagaagaagc ggaaagtcga cccccccacc ttcaccttca acttcaacaa
      421 tgagccttgg gtgcggggca gacacgagac ctacctgtgc tatgaggtgg agcggatgca
      481 caacgacaca tgggtgctgc tgaaccagag gaggggcttt ctgtgcaatc aggcccccca
      541 caagcacggc ttcctggagg gcaggcacgc agagctgtgc ttcctggacg tgatcccatt
      601 ctggaagctg gacctggatc aggactaccg cgtgacctgt tttacatctt ggagcccctg
      661 cttctcctgt gcccaggaga tggccaagtt tatctccaag aacaagcacg tgtctctgtg
      721 catcttcacc gccaggatct acgacgatca gggcaggtgt caggagggac tgcgcacact
      781 ggccgaggca ggagccaaga tcagcatcat gacctatagc gagtttaagc actgctggga
      841 tacattcgtg gaccaccagg gctgtccatt ccagccctgg gatggcctgg acgagcactc
      901 ccaggacctg tctggcaggc tgagggccat cctgcagaac caggagaatt ctggaggatc
      961 tagcggagga tcctctggca gcgagacacc aggaacaagc gagtcagcaa caccagagag
     1021 cagtggcggc agcagcggcg gcagcgacaa gaagtacagc atcggcctgg ccatcggcac
     1081 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
     1141 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt
     1201 cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac
     1261 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
     1321 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
     1381 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
     1441 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct
     1501 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
     1561 cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa
     1621 ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc
     1681 tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa
     1741 gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa
     1801 gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga
     1861 cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc
     1921 caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac
     1981 caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac
     2041 cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga
     2101 ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta
     2161 caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct
     2221 gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca
     2281 gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct
     2341 gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg
     2401 ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat
     2461 caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga
     2521 gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct
     2581 gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg
     2641 aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt
     2701 caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga
     2761 gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac
     2821 ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga
     2881 ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga
     2941 ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg
     3001 gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa
     3061 gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt
     3121 catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt
     3181 gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat
     3241 taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg
     3301 gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg
     3361 acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag
     3421 ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct
     3481 gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct
     3541 gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga
     3601 caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga
     3661 agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac
     3721 ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa
     3781 ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca
     3841 gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt
     3901 gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta
     3961 caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt
     4021 gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta
     4081 caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac
     4141 cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc
     4201 caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt
     4261 gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa
     4321 tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa
     4381 gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg
     4441 cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa
     4501 gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag
     4561 cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga
     4621 cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat
     4681 gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt
     4741 gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga
     4801 gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat
     4861 cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc
     4921 ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt
     4981 taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg
     5041 gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac
     5101 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggtgacagcg gcgggagcgg
     5161 cgggagcggg gggagcacta atctgagcga catcattgag aaggagactg ggaaacagct
     5221 ggtcattcag gagtccatcc tgatgctgcc tgaggaggtg gaggaagtga tcggcaacaa
     5281 gccagagtct gacatcctgg tgcacaccgc ctacgacgag tccacagatg agaatgtgat
     5341 gctgctgacc tctgacgccc ccgagtataa gccttgggcc ctggtcatcc aggattctaa
     5401 cggcgagaat aagatcaaga tgctgagcgg aggatccgga ggatctggag gcagcaccaa
     5461 cctgtctgac atcatcgaga aggagacagg caagcagctg gtcatccagg agagcatcct
     5521 gatgctgccc gaagaagtcg aagaagtgat cggaaacaag cctgagagcg atatcctggt
     5581 ccataccgcc tacgacgaga gtaccgacga aaatgtgatg ctgctgacat ccgacgcccc
     5641 agagtataag ccctgggctc tggtcatcca ggattccaac ggagagaaca aaatcaaaat
     5701 gctgtctggc ggctcaaaaa gaaccgccga cggcagcgaa ttcgagccca agaagaagag
     5761 gaaagtctaa ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg
     5821 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5881 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5941 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     6001 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     6061 agaaccagct ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca
     6121 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6181 agcataaagt gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6241 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6301 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6361 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6421 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6481 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6541 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6601 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6661 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6721 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6781 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6841 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6901 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6961 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7021 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7081 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7141 actcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7201 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7261 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7321 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7381 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7441 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7501 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7561 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7621 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7681 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7741 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7801 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7861 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7921 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7981 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8041 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8101 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8161 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8221 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     8281 tcgggagatc gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc
     8341 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     8401 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     8461 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
     8521 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     8581 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     8641 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     8701 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     8761 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     8821 gcccagtaca tgacctta
//
