LOCUS       sequence-320311-        9283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pdpy30::loxP::mcherry::let-858terminator::loxP::GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   Mao S, Qi Y, Zhu H, Huang X, Zou Y, Chi T
  TITLE     A Tet/Q Hybrid System for Robust and Versatile Control of Transgene 
            Expression in C. elegans.
  JOURNAL   iScience. 2019 Jan 25;11:224-237. doi: 10.1016/j.isci.2018.12.023. 
            Epub 2018 Dec 27.
  PUBMED    30634168
REFERENCE   2  (bases 1 to 9283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-01-25"; volume: "11:224-237. doi"; pages: " 
            10.1016/j.isci.2018.12.023. Epub 2018 Dec 27"
COMMENT     Sequence Label: TC646
FEATURES             Location/Qualifiers
     source          1..9283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     283..299
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     283..299
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(1580..1613)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2911..2944)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(3006..3034)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3782..3802
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      3846..4169
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(4282..4299)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4282..4298)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5120..5914
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5174..5193)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5784..5803
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6615..6631
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6616..6632
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7287..7309
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7347..7365)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7433..7537
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7538..7606,7607..8398)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7756..7775)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8569..9157
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9058..9077
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcaccgtacg tctcgaggaa ttctatcagg tgtacaagta tgaaatgtcg tttcgaacat
       61 atagatgtct cgcaaacgta aatatttatc gattgtcata aaactttgac cttgtgaagt
      121 gtcaaccttg actgtcgaac caccatagtt tggcgcgaat tgagcgtcat aattgtttac
      181 tctcagtgca gtcaacatgt cgagtttcgt gccgaataaa gagcaaacgc ggacagtatt
      241 aattttctgt tttcatttga agaaaacagc tgcggccgga tccaggaaac agctatgacc
      301 atgattacgc caagctacgt aatacgactc actagtgggc agatcttcga atgcatcaaa
      361 agtgtcttat ctagattttt aaaaatttgt tcttctccac ttgttctctt aataaaataa
      421 attctaaatt ttgaacacta attagtgcac taatcaatgt tttgaatttt gaataaaata
      481 attttaaaat tttaaatgtt gtaaattttt agtgatactg taggtacaag gaaactattt
      541 tattgaattc ttgatcaaag aaggtaggca gtttatataa ataatcttat tgttagatac
      601 cattttgaat atatgtttca gttccagaaa aataagctat acatagtatc gatttggaac
      661 atatataacg aaaacagacg tcatgaaatc acacattgga aaagtgagaa agttgttgag
      721 tagaggaggt gacaaaagtg agatgccaac tcacttatcc attctactcg ttttgtgctc
      781 ttttccactt tctgatgtct gctcatcttc ttattcttca agcgttttgt ctattctcac
      841 acctctccaa tctttcagtt tctgttttta atgtctagac acgcgtgtaa cgtacaacat
      901 aaatgtttga cgtcaaccag atgtagtaca ataaaaaata tattcagatt acctagaaaa
      961 ttacttcggt catgaccacc aattttttga tttttcgatt ttcgaaaccg acatcgttga
     1021 agaacattca ggtcataatt ttttatttga aaaataatgt tttctatgta ttaattcggt
     1081 ttagtcgatc gaatatgtac agttgagaat tactagagta cggtgcgcaa gtatgcaaac
     1141 ttgcgtaacg gtgtttacac atttgtcacg tttctctgcg tctcttcgtc tcaacaatgc
     1201 ttgcactccg tcacattttc gaattgtcgg ccgatatcgg tatttagata aattcagtgc
     1261 tctccttttt ttctccattt tactttcatc aatctttttt aaaaatcaat attcagcaaa
     1321 ctattgaaaa attaattcaa aaaacacgac tggtcggaag ttatttgcat aatcaataga
     1381 aatttcgttt tccgctcgct gtcagtgttt tgtcctgttt gtatttccca gtgcactttt
     1441 cgagtggctc aagtttttat ctattttcct gcaaacttgc atatattgtt ttcttccaga
     1501 agcctttgat atctcaataa ttttatactt gtttcagaaa tcgagcaaaa accaaggtat
     1561 gtttcgaatg atactaataa taacttcgta tagcatacat tatacgaagt tataacataa
     1621 catagaacat tttcagatgg tctcaaaggg tgaagaagat aacatggcaa ttattaaaga
     1681 gtttatgcgt ttcaaggtgc atatggaggg atctgtcaat gggcatgagt ttgaaattga
     1741 aggtgaagga gaaggccgac catatgaggg aacacaaacc gcaaaactaa aggtaagttt
     1801 aaacatatat atactaacta accctgatta tttaaatttt caggtaacta aaggcggacc
     1861 attaccattc gcctgggaca tcctctctcc acagttcatg tatggaagta aagcttatgt
     1921 taaacatccg gcagatatac cagattattt gaaactttca ttcccggagg gttttaagtg
     1981 ggaacgcgta atgaattttg aagacggagg agttgttaca gtgacgcaag actcaaggta
     2041 agtttaaaca gttcggtact aactaaccat acatatttaa attttcagcc tccaagatgg
     2101 agaatttatt tataaagtca aacttcgagg aacgaatttc ccctcggatg gacctgttat
     2161 gcagaagaag actatgggat gggaagcttc aagtgaaaga atgtaccctg aagacggtgc
     2221 tcttaaggga gagattaaac aacgtcttaa attgaaagat ggaggacatt acgatgctga
     2281 ggtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc aggtgaagac
     2341 aacttacaaa gccaaaaaac cagttcagct gccaggagcg tacaatgtta atattaaact
     2401 ggatatcacc tcccacaacg aggattacac tatcgttgag caatatgaaa gagctgaagg
     2461 gcggcactcg acaggtggca tggatgaatt gtataagtag ggatgatcga cgccgacgtc
     2521 gttgaatttt caaattttaa atactgaata tttgtttttt ttcctattat ttatttattc
     2581 tctttgtgtt ttttttcttg ctttctaaaa aattaattca atccaaatct aaacattttt
     2641 ttttctcttt ccgtctccca attcgtattc cgctcctctc atctgaacac aatgtgcaag
     2701 tttatttatc ttctcgcttt catttcatta ggacgtgggg ggaattggtg gaagggggaa
     2761 acacacaaaa ggatgatgga aatgaaataa ggacacacaa tatgcaacaa cattcaattc
     2821 agaaatatgg aggaaggttt aaaagaaaac ataaaaatat atagaggagg aaggaaaact
     2881 aaggtaagtt taaactttct catactaata ataacttcgt atagcataca ttatacgaag
     2941 ttatattaac taacgcgctc tatttaaatt ttcagatgag taaaggagaa gaacttttca
     3001 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
     3061 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
     3121 ctactggaaa actacctgtt ccatgggtaa gtttaaacat atatatacta actaaccctg
     3181 attatttaaa ttttcagcca acacttgtca ctactttctg ttatggtgtt caatgcttct
     3241 cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt
     3301 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtaagttta
     3361 aacagttcgg tactaactaa ccatacatat ttaaattttc aggtgctgaa gtcaagtttg
     3421 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
     3481 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
     3541 acaaacaaaa gaatggaatc aaagttgtaa gtttaaacat gattttacta actaactaat
     3601 ctgatttaaa ttttcagaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
     3661 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     3721 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     3781 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     3841 aatagatgca agatcctttc aagcattccc ttcttctcta tcactcttct ttctttttgt
     3901 caaaaaattc tctcgctaat ttatttgctt ttttaatgtt attattttat gactttttat
     3961 agtcactgaa aagtttgcat ctgagtgaag tgaatgctat caaaatgtga ttctgtctga
     4021 tgtactttca caatctctct tcaattccat tttgaagtgc tttaaacccg aaaggttgag
     4081 aaaaatgcga gcgctcaaat atttgtattg tgttcgttga gtgacccaac aaaaagagga
     4141 aactttattg tgccgccaag aaaaaagtct cctgcaggat atctggatcc acgaagcttc
     4201 ccatggtgac gtcaccggtt ctagatacct aggtgagctc tggtaccctc tagtcaaggc
     4261 cttaagtgag tcgtattacg gactggccgt cgttttacag gccggatggg ccatggcacc
     4321 ttaacatttc taaattttag tttaataaaa aaattttaaa ataatacgag ttttgagact
     4381 aactttcaat aaaaaccaaa aaattgcttc aaaaaagagc tcgcgccgat ttacgataaa
     4441 cacagcgcgc tacgacactc cgaaaaccgc gccgcaccat tacattctcc gccccaccgc
     4501 acggtttgcg gaaggcgatg aaattgctgg gcctaaaatt ttaacccttt ttttcccgtt
     4561 tttcaactgt tttcccgatt ttttcgatat ttttttcagg tttttagtta gaaaagtata
     4621 taaaaattgc attatttgta gttttcgctc gtaatttgtg atagaaaacg gccaatttgc
     4681 ctagatttgt gattctacca agtggaattt ttgggaaaat atcaatattc cacgattttt
     4741 attcaaatca taacgtttcg aaattatcag cttaaataaa actaaaataa ataataaaat
     4801 actaaaaacc ctgaatttct cggttctcgg ttcagcttga gttcgttcta tacacgttat
     4861 cgatttttgt catttcgttt aatcatgggt tttgattcat tttaaatatg aaatttgatt
     4921 tattcctcgt ttcgaagttg gtttgtcttg tccggccggt aaacaaatgt tattaaaaaa
     4981 aataataaat ttcgaaattt ttcgtaatgt tccgcaatcg tgtttttccg tttatctcgt
     5041 cttcccttcc ttcaaatata ctatttattt tctcaattca catattttca gccagccctg
     5101 ctcaacaagt ggaataaaaa tgattgaaca agatggattg cacgcaggtt ctccggccgc
     5161 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     5221 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     5281 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     5341 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     5401 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     5461 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     5521 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     5581 tcaggatgat ctggacgaag agcatcaggg gctcgcacca gccgaactgt tcgccaggct
     5641 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     5701 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     5761 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     5821 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     5881 cgccttctat cgccttcttg acgagttctt ctgagtccaa ttactcttca acatccctac
     5941 atgctctttc tccctgtgct cccaccccct atttttgtta ttatcaaaaa acttctctta
     6001 atttctttgt tttttagctt cttttaagcc acctctaaca atgaaattgt gtagattcaa
     6061 aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata
     6121 ataattctat cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttacttct
     6181 gataaatttt tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta
     6241 cgtttcagtt tatgaccgca atttttattt cttcgcacgt ctgggcctct catgacgtca
     6301 aatcatgctc atcgtgaaaa agttttggag tatttttgga atttttcaat caagtgaaag
     6361 tttatgaaat taattttcct gcttttgctt tttggggttt cccctattgt ttgtcaagat
     6421 ttcgaggacg gcgtttttct tgctaaaatc acaagtattg atgagcacga tgcaagaaag
     6481 atcggaagaa ggtttgggtt tgaggctcag tggaaggtga gtagaagttg ataatttgaa
     6541 agtggagtag tgtctatggg gtttttgcct taaatgacag aatacattcc caatatacca
     6601 aacataactg tttctcgagg tcgacggtat cgataagctt gatccggatc tgcggcttac
     6661 tcaccagacc tggccccatc cgattaccac ctattcgctt cgatgggaca cgcactcgct
     6721 gagcagcgct tcgattctta cgaaagtgtg aaaaaatggc tcgatgaatg gttcgccgca
     6781 aaagacgatg agttctactg gcgtggaatc cacaaattgc ccgagagatg ggaaaaatgt
     6841 gtagctagcg acggcaaata ctttgaataa atgatttttt ctttttccac aaaatttaac
     6901 gtgttttttg atttaaaaaa aacgacattt catacttgta cacctgatat ctagatacct
     6961 aggtgagctc tggtaccccc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     7021 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     7081 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     7141 ttcacaccgc atatggtgca ctctcagtgc aatctgctct gatgccgcat agttaagcca
     7201 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     7261 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     7321 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     7381 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     7441 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7501 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7561 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7621 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7681 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7741 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7801 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7861 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7921 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7981 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8041 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8101 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8161 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8221 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8281 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8341 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8401 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8461 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8521 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8581 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8641 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8701 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8761 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8821 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8881 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8941 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9001 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9061 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9121 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9181 acggttcctg gtcttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9241 ttctgtggat aaccgtatta ccgcctttga gtgagctgat acc
//
