LOCUS       sequence-320314-        7370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TRE::nCre, inducible nCre expression when co-express with rtTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Mao S, Qi Y, Zhu H, Huang X, Zou Y, Chi T
  TITLE     A Tet/Q Hybrid System for Robust and Versatile Control of Transgene 
            Expression in C. elegans.
  JOURNAL   iScience. 2019 Jan 25;11:224-237. doi: 10.1016/j.isci.2018.12.023. 
            Epub 2018 Dec 27.
  PUBMED    30634168
REFERENCE   2  (bases 1 to 7370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-01-25"; volume: "11:224-237. doi"; pages: " 
            10.1016/j.isci.2018.12.023. Epub 2018 Dec 27"
COMMENT     Sequence Label: TC872
FEATURES             Location/Qualifiers
     source          1..7370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     283..299
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     283..299
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    365..383
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    401..419
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    437..455
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    473..491
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    509..527
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    545..563
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    581..599
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             883..903
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             904..1932
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     terminator      1933..2256
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(2369..2386)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2369..2385)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3207..4001
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3261..3280)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3871..3890
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4702..4718
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4703..4719
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5374..5396
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5434..5452)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5520..5624
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5625..5693,5694..6485)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5843..5862)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6656..7244
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7145..7164
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcaccgtacg tctcgaggaa ttctatcagg tgtacaagta tgaaatgtcg tttcgaacat
       61 atagatgtct cgcaaacgta aatatttatc gattgtcata aaactttgac cttgtgaagt
      121 gtcaaccttg actgtcgaac caccatagtt tggcgcgaat tgagcgtcat aattgtttac
      181 tctcagtgca gtcaacatgt cgagtttcgt gccgaataaa gagcaaacgc ggacagtatt
      241 aattttctgt tttcatttga agaaaacagc tgcggccgga tccaggaaac agctatgacc
      301 atgattacgc caagctacgt aatacgactc actagtgggc agatcttcga atgcatgagt
      361 ttactcccta tcagtgatag agaacgtatg aagagtttac tccctatcag tgatagagaa
      421 cgtatgcaga ctttactccc tatcagtgat agagaacgta taaggagttt actccctatc
      481 agtgatagag aacgtatgac cagtttactc cctatcagtg atagagaacg tatctacagt
      541 ttactcccta tcagtgatag agaacgtata tccagtttac tccctatcag tgatagagaa
      601 cgtatgtcga ggtaggcgtg atcgattttt tgcaaattac gagcgttgta gggggcggag
      661 cgataggtcc tataggtttt ggtatatcat cattcattca ttcattggta cattcattta
      721 cccaccttcc tctttctgag cttctctgga gttctgtgct tcctttttcc cttatcttta
      781 tactgtaatt tttaactttc agaaggaccc aaaggtatgt ttcgaatgat actaacataa
      841 catagaacat tttcaggaga ttggagagac accatgggcg cacccaagaa gaagaggaag
      901 gtgtccaatt tactgaccgt acaccaaaat ttgcctgcat taccggtcga tgcaacgagt
      961 gatgaggttc gcaagaacct gatggacatg ttcagggatc gccaggcgtt ttctgagcat
     1021 acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac
     1081 cggaaatggt ttcccgcaga acctgaagat gttcgcgatt atcttctata tcttcaggcg
     1141 cgcggtctgg cagtaaaaac tatccagcaa catttgggcc agctaaacat gcttcatcgt
     1201 cggtccgggc tgccacgacc aagtgacagc aatgctgttt cactggttat gcggcggatc
     1261 cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact
     1321 gatttcgacc aggttcgttc actcatggaa aatagcgatc gctgccagga tatacgtaat
     1381 ctggcatttc tggggattgc ttataacacc ctgttacgta tagccgaaat tgccaggatc
     1441 agggttaaag atatctcacg tactgacggt gggagaatgt taatccatat tggcagaacg
     1501 aaaacgctgg ttagcaccgc aggtgtagag aaggcactta gcctgggggt aactaaactg
     1561 gtcgagcgat ggatttccgt ctctggtgta gctgatgatc cgaataacta cctgttttgc
     1621 cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca gccagctatc aactcgcgcc
     1681 ctggaaggga tttttgaagc aactcatcga ttgatttacg gcgctaagga tgactctggt
     1741 cagagatacc tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg agatatggcc
     1801 cgcgctggag tttcaatacc ggagatcatg caagctggtg gctggaccaa tgtaaatatt
     1861 gtcatgaact atatccgtaa cctggatagt gaaacagggg caatggtgcg cctgctggaa
     1921 gatggcgatt agatgcaaga tcctttcaag cattcccttc ttctctatca ctcttctttc
     1981 tttttgtcaa aaaattctct cgctaattta tttgcttttt taatgttatt attttatgac
     2041 tttttatagt cactgaaaag tttgcatctg agtgaagtga atgctatcaa aatgtgattc
     2101 tgtctgatgt actttcacaa tctctcttca attccatttt gaagtgcttt aaacccgaaa
     2161 ggttgagaaa aatgcgagcg ctcaaatatt tgtattgtgt tcgttgagtg acccaacaaa
     2221 aagaggaaac tttattgtgc cgccaagaaa aaagtctcct gcaggatatc tggatccacg
     2281 aagcttccca tggtgacgtc accggttcta gatacctagg tgagctctgg taccctctag
     2341 tcaaggcctt aagtgagtcg tattacggac tggccgtcgt tttacaggcc ggatgggcca
     2401 tggcacctta acatttctaa attttagttt aataaaaaaa ttttaaaata atacgagttt
     2461 tgagactaac tttcaataaa aaccaaaaaa ttgcttcaaa aaagagctcg cgccgattta
     2521 cgataaacac agcgcgctac gacactccga aaaccgcgcc gcaccattac attctccgcc
     2581 ccaccgcacg gtttgcggaa ggcgatgaaa ttgctgggcc taaaatttta accctttttt
     2641 tcccgttttt caactgtttt cccgattttt tcgatatttt tttcaggttt ttagttagaa
     2701 aagtatataa aaattgcatt atttgtagtt ttcgctcgta atttgtgata gaaaacggcc
     2761 aatttgccta gatttgtgat tctaccaagt ggaatttttg ggaaaatatc aatattccac
     2821 gatttttatt caaatcataa cgtttcgaaa ttatcagctt aaataaaact aaaataaata
     2881 ataaaatact aaaaaccctg aatttctcgg ttctcggttc agcttgagtt cgttctatac
     2941 acgttatcga tttttgtcat ttcgtttaat catgggtttt gattcatttt aaatatgaaa
     3001 tttgatttat tcctcgtttc gaagttggtt tgtcttgtcc ggccggtaaa caaatgttat
     3061 taaaaaaaat aataaatttc gaaatttttc gtaatgttcc gcaatcgtgt ttttccgttt
     3121 atctcgtctt cccttccttc aaatatacta tttattttct caattcacat attttcagcc
     3181 agccctgctc aacaagtgga ataaaaatga ttgaacaaga tggattgcac gcaggttctc
     3241 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     3301 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     3361 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     3421 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     3481 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     3541 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     3601 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     3661 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcaccagcc gaactgttcg
     3721 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     3781 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     3841 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     3901 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     3961 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agtccaatta ctcttcaaca
     4021 tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaact
     4081 tctcttaatt tctttgtttt ttagcttctt ttaagccacc tctaacaatg aaattgtgta
     4141 gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc
     4201 attaataata attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt
     4261 tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca
     4321 tatgttacgt ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat
     4381 gacgtcaaat catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa
     4441 gtgaaagttt atgaaattaa ttttcctgct tttgcttttt ggggtttccc ctattgtttg
     4501 tcaagatttc gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc
     4561 aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata
     4621 atttgaaagt ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa
     4681 tataccaaac ataactgttt ctcgaggtcg acggtatcga taagcttgat ccggatctgc
     4741 ggcttactca ccagacctgg ccccatccga ttaccaccta ttcgcttcga tgggacacgc
     4801 actcgctgag cagcgcttcg attcttacga aagtgtgaaa aaatggctcg atgaatggtt
     4861 cgccgcaaaa gacgatgagt tctactggcg tggaatccac aaattgcccg agagatggga
     4921 aaaatgtgta gctagcgacg gcaaatactt tgaataaatg attttttctt tttccacaaa
     4981 atttaacgtg ttttttgatt taaaaaaaac gacatttcat acttgtacac ctgatatcta
     5041 gatacctagg tgagctctgg tacccccctg gcgttaccca acttaatcgc cttgcagcac
     5101 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     5161 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
     5221 gcggtatttc acaccgcata tggtgcactc tcagtgcaat ctgctctgat gccgcatagt
     5281 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     5341 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5401 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     5461 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     5521 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5581 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5641 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5701 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5761 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5821 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5881 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5941 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6001 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6061 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6121 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6181 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6241 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6301 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6361 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6421 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6481 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6541 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6601 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6661 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6721 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6781 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     6841 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6901 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6961 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7021 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7081 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7141 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7201 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7261 cctttttacg gttcctggtc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7321 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
//
