LOCUS       sequence-320317-        8318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  YFP fusion of Sec24C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Stephens DJ, Lin-Marq N, Pagano A, Pepperkok R, Paccaud JP
  TITLE     COPI-coated ER-to-Golgi transport complexes segregate from COPII in 
            close proximity to ER exit sites.
  JOURNAL   J Cell Sci. 2000 Jun;113 ( Pt 12):2177-85.
  PUBMED    10825291
REFERENCE   2  (bases 1 to 8318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2000-06"; volume: "113 ( Pt 12)"; pages: "2177-85"
COMMENT     Sequence Label: pEYFP-Sec24C
FEATURES             Location/Qualifiers
     source          1..8318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5106..5227
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5143..5162)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5197..5216
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5234..5689)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5371..5392)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5583..5602
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5716..5820
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5822..6179
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5822..6013
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5845..5865)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6030..6165
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6214..7008
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6214..7008
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6268..6287)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6878..6897
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7196..7215)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7240..7287
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7616..8204
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8105..8124
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacgaattc
     1381 aacgtcaacc agtcagttcc acctgtgcca ccatttgggc agccccagcc catctaccca
     1441 gggtatcatc agtccagcta tggtgggcaa tcagggtcca cagcccccgc cattccctat
     1501 ggagcctaca atggcccagt accaggctat cagcaaacac ctccccaagg tatgtcaaga
     1561 gccccacctt cctcgggggc acctccagcc tcaacagcac aggctccttg tggccaggct
     1621 gcatatggcc agtttggcca aggagatgta cagaatgggc caagctccac tgttcagatg
     1681 caaaggctgc ctgggtctca gccatttggg tccccattgg cccctgtggg caaccagcca
     1741 cctgtgcttc agccctatgg ccctcccccg acaagtgcac aggtggctac gcagctgtct
     1801 ggaatgcaga tcagcggtgc tgtggcccca gcccctcctt cttcagggct gggctttggc
     1861 ccaccaacat cgctggcttc agcctcagga agtttcccta actctggtct gtatggctcc
     1921 tatcctcagg gccaggctcc tccccttagc caggcccaag gtcatcctgg gatccagact
     1981 ccccagcgat ctgccccatc acaggcctcc agcttcacac ccccagcttc agggggtcct
     2041 cggctgcctt cgatgactgg tccactcctg cctggacaga gttttggagg gccctcagtg
     2101 agccagccca accatgtgtc ttcacctcct caagctctgc cccctggcac ccagatgact
     2161 gggcccctgg gaccactgcc acctatgcac tccccgcagc agccaggcta tcagccccaa
     2221 caaaatggtt ccttcggacc agcccggggc cctcagtcta attatggagg cccctaccca
     2281 gcagcaccca cctttggcag tcagcctggg cctcctcagc cactgcctcc taagcgcctg
     2341 gaccctgatg ccatcccaag ccctattcag gtcattgaag atgacaggaa caaccggggt
     2401 acagagccat ttgttactgg agtacggggc caggtgccac ccttagtcac taccaacttc
     2461 ctggtgaaag accaagggaa tgcaagtccc cgatacatcc gatgtacatc ctataatatc
     2521 ccttgcacat ctgacatggc taagcaggct caggtgcccc tggcagcagt catcaaaccg
     2581 ctggcaaggc tgcccccaga ggaggcttca ccgtatgttg tggaccatgg ggaatctggc
     2641 cctttgcgct gcaaccgctg caaagcatac atgtgtccct tcatgcagtt cattgaagga
     2701 gggaggcgtt tccagtgctg tttttgcagc tgtatcaatg atgttccccc ccagtatttt
     2761 cagcacctgg atcataccgg caaacgtgtg gatgcttatg accgccctga gctatccctg
     2821 ggctcttatg aattcttggc cactgtagat tactgcaaga acaataagtt ccccagccct
     2881 cctgccttta tcttcatgat tgacgtctcc tacaatgcca tcaggactgg tcttgttagg
     2941 ctcctctgtg aggagctcaa gtcactgtta gactttctac ctagggaggg tggggcagaa
     3001 gagtcagcaa tccgcgttgg ctttgtcacc tacaataagg tgctccactt ctataatgtg
     3061 aagagctcat tggcccagcc acagatgatg gttgtgtctg atgtggctga catgtttgtg
     3121 ccactgctgg atggcttcct ggtcaacgtc aatgagtctc gggcagttat caccagctta
     3181 ttggatcaga ttccagaaat gtttgcagac acaagggaaa cagagacagt atttgtacca
     3241 gttatccagg ctggaatgga ggctctgaag gctgctgagt gtgcagggaa gctctttcta
     3301 ttccatacat ccctgcccat tgcagaggcc ccagggaaac tgaagaacag agatgacagg
     3361 aagctgatca atacagacaa ggagaagact ctgttccagc ctcagacagg tgcctatcag
     3421 accctggcca aagagtgtgt ggcccaaggc tgctgtgtag atctctttct cttccctaac
     3481 cagtatgtgg atgtggccac actctctgtt gtgccccagc tcactggtgg ctctgtctac
     3541 aaatatgctt cctttcaggt ggagaacgac caggagcggt tcctgagtga cctgcgtcgt
     3601 gatgtccaga aggttgttgg ctttgatgct gtgatgcggg tccggacaag cactggtatc
     3661 cgtgctgtag atttctttgg agctttctac atgagcaaca cgacagatgt ggagctggct
     3721 gggctagatg gggacaaaac agtgactgtg gagttcaagc atgacgatcg gctcaatgaa
     3781 gagagcggag ctctcctgca gtgtgccctg ctttacacca gctgtgcagg gcagcgtcgg
     3841 ctccgcatcc ataatctggc cctgaactgc tgcacccagc tggctgatct atatcgaaac
     3901 tgtgagactg acacgctcat caactacatg gccaagtttg catatcgggg agtcctgaat
     3961 agccctgtga aggctgttcg tgacacgctc atcacccagt gtgcccagat cctggcctgt
     4021 tacagaaaga actgtgctag cccctcctct gcaggacagt tgatccttcc tgagtgcatg
     4081 aagctactcc cagtttacct gaactgtgtg ttgaagagtg atgtcctgca gcctggagct
     4141 gaagtcacta ctgatgaccg tgcctatgtc cgacagctag ttacctccat ggatgtgact
     4201 gagaccaatg tcttcttcta ccctcggctc ttacctttga caaagtctcc cgttgagagt
     4261 actaccgaac caccagcagt tcgagcctct gaagagcgtc taagcaatgg ggatatatat
     4321 ttactggaga atgggctcaa cctcttcctc tgggtgggag caagcgtcca acagggtgtt
     4381 gtccagagcc ttttcagcgt ctcctccttc agtcagatca ccagtggttt gagtgttctg
     4441 ccagttctgg ataatccact gtccaagaag gttcgaggcc tcattgatag cttacgggca
     4501 cagagatccc ggtacatgaa gcttaccgtg gtgaaacagg aagacaagat ggagatgctg
     4561 ttcaagcact tcctggtgga agacaagagt ctgagtgggg gagcatctta tgtggacttt
     4621 ctctgtcata tgcacaagga gattcggcag ctactgagct aaagcaagtg ggtaaatggc
     4681 atagggccca ggctagcttc cagaaagcac cccaggatgt cagagaaatt gggacagtaa
     4741 catatcttat gtaagctgac ctcagtctct ctggggggag ggggagatat aaggagacac
     4801 cttctttctg ggctcaagta tcctgccact ctgtcatgtc ctgctgatgg aaggtgcccc
     4861 tgttccctca ttctaccctc tttttcctgc taatcctgtc ataatgaatg tagcttctca
     4921 gttcactgta tatgattcgg tattgggggt ttggaggcac ccagaccctg gcaatgggat
     4981 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg
     5041 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     5101 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5161 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5221 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     5281 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     5341 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     5401 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     5461 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     5521 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     5581 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     5641 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     5701 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5761 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5821 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     5881 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     5941 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6001 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     6061 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     6121 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     6181 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     6241 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     6301 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     6361 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     6421 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     6481 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     6541 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     6601 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     6661 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     6721 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     6781 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     6841 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     6901 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     6961 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     7021 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     7081 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     7141 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     7201 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     7261 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     7321 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     7381 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     7441 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     7501 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7741 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8281 cccctgattc tgtggataac cgtattaccg ccatgcat
//
