LOCUS       sequence-320355-       11106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian CG-to-GC base editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, 
            Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, 
            Adamson B, Liu DR
  TITLE     Efficient C*G-to-G*C base editors developed using CRISPRi screens, 
            target-library analysis, and machine learning.
  JOURNAL   Nat Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z.
  PUBMED    34183861
REFERENCE   2  (bases 1 to 11106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z."
COMMENT     Sequence Label: pCMV_UdgX-Anc689-UdgX-nCas9-RBMX
FEATURES             Location/Qualifiers
     source          1..11106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(684..707,708..710,711..4784)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             684..4784
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6084..6104
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6113..6130
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6153..6170)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6159..6383
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6454..6470)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6454..6470)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6467..6489)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6478..6494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6502..6508,6509..6526,6527..6532))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6547..6568
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6685..6702)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6856..7444)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6936..6955)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7615..8406,8407..8475))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8238..8257
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8476..8580)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8659..8678)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8850..9229
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9230..9433
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9384..9404
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9475..9494
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9475..9493
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9543..9563
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tggctgagct ggctgccgcc gctggagagt gtagaggatg cgggctgtac cgggatgcca
       61 cacaggccgt gttcggagca ggcggcagga gcgcccgcat catgatgatc ggcgagcagc
      121 caggcgataa ggaggacctg gccggcctgc catttgtggg accagcaggc cggctgctgg
      181 acagagccct ggaggccgcc gacatcgata gggacgccct gtacgtgacc aacgccgtga
      241 agcacttcaa gtttacacgc gccgcaggag gcaagaggag aatccacaag accccctctc
      301 ggacagaggt ggtggcctgc agaccttggc tgatcgccga gatgaccagc gtggagccag
      361 atgtggtggt gctgctggga gcaacagcag caaaggccct gctgggcaat gacttcaggg
      421 tgacccagca caggggagag gtgctgcacg tggacgatgt gccaggcgat cctgccctgg
      481 tggcaaccgt gcaccctagc tccctgctga gaggcccaaa ggaggagcgg gaatccgcct
      541 tcgctgggct ggtggacgac ctgagagtgg ctgccgatgt gagacctagc ggtggtagtt
      601 ctggcggtag ctcaggatct gaaactcccg gcacatctga aagcgcgact cccgaatcat
      661 ctggtggatc ttcaggtgga agtgacaaga agtacagcat cggcctggcc atcggcacca
      721 actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg
      781 tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg
      841 acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca
      901 gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg
      961 acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg
     1021 agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca
     1081 ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga
     1141 tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc
     1201 tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc
     1261 agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg
     1321 ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga
     1381 agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga
     1441 gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg
     1501 acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca
     1561 agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca
     1621 aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc
     1681 tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc
     1741 agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca
     1801 agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga
     1861 acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga
     1921 tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga
     1981 aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc
     2041 ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca
     2101 ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc
     2161 ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc
     2221 tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa
     2281 tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca
     2341 agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt
     2401 gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat
     2461 accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg
     2521 acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg
     2581 aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc
     2641 ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc
     2701 agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca
     2761 tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt
     2821 ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta
     2881 agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc
     2941 acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac
     3001 agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc
     3061 agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt
     3121 actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt
     3181 ccgactacga tgtggaccat atcgtgcctc agagctttct gaaggacgac tccatcgaca
     3241 acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag
     3301 aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc
     3361 agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg
     3421 ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga
     3481 tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga
     3541 aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca
     3601 aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg
     3661 gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca
     3721 aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg
     3781 ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca
     3841 acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt
     3901 gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata
     3961 tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga
     4021 ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct
     4081 tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt
     4141 ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct
     4201 tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc
     4261 tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc
     4321 tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga
     4381 acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc
     4441 agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca
     4501 gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct
     4561 acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta
     4621 ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga
     4681 agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg
     4741 gcctgtacga gacacggatc gacctgtctc agctgggagg tgactctgga ggatctagcg
     4801 gaggatcctc tggcagcgag acaccaggaa caagcgagtc agcaacacca gagagcagtg
     4861 gcggcagcag cggcggcagc atggttgaag cagatcgccc aggaaagctc ttcattggtg
     4921 ggcttaatac ggaaacaaat gagaaagctc ttgaagcagt atttggcaaa tatggacgaa
     4981 tagtggaagt actcttgatg aaagaccgtg aaaccaacaa atcaagagga tttgcttttg
     5041 tcacctttga aagcccagca gacgctaagg atgcagccag agacatgaat ggaaagtcat
     5101 tagatggaaa agccatcaag gtggaacaag ccaccaaacc atcatttgaa agtggtagac
     5161 gtggaccgcc tccacctcca agaagtagag gccctccaag aggtcttaga ggtggaagag
     5221 gaggaagtgg aggaaccagg ggacctccct cacggggagg acacatggat gacggtggat
     5281 attccatgaa ttttaacatg agttcttcca ggggaccact cccagtaaaa agaggaccac
     5341 caccaagaag tgggggtcct cctcctaaga gatctgcacc ttcaggacca gttcgcagta
     5401 gcagtggaat gggaggaaga gctcctgtat cacgtggaag agatagttat ggaggtccac
     5461 ctcgaaggga accgctgccc tctcgtagag atgtttattt gtccccaaga gatgatgggt
     5521 attctactaa agacagctat tcaagcagag attacccaag ttctcgtgat actagagatt
     5581 atgcaccacc accacgagat tatacttacc gtgattatgg tcattccagt tcacgtgatg
     5641 actatccatc aagaggatat agcgatagag atggatatgg tcgtgatcgt gactattcag
     5701 atcatccaag tggaggttcc tacagagatt catatgagag ttatggtaac tcacgtagtg
     5761 ctccacctac acgagggccc ccgccatctt atggtggaag cagtcgctat gatgattaca
     5821 gcagctcacg tgacggatat ggtggaagtc gagacagtta ctcaagcagc cgaagtgatc
     5881 tctactcaag tggtcgtgat cgggttggca gacaagaaag agggcttccc ccttctatgg
     5941 aaagggggta ccctcctcca cgtgattcct acagcagttc aagccgcgga gcaccaagag
     6001 gtggtggccg tggaggaagc cgatctgata gagggggagg cagaagcaga tacaaaagaa
     6061 ccgccgacgg cagcgaattc gagcccaaga agaagaggaa agtctaaccg gtcatcatca
     6121 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     6181 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     6241 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     6301 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     6361 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgatacc
     6421 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6481 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctaggg
     6541 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6601 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggaa gaggcggttt
     6661 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6721 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6781 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6841 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6901 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6961 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7021 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7081 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7141 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7201 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7261 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7321 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7381 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7441 tcaagaagat cctttgatct tttctacggg gtctgacact cagtggaacg aaaactcacg
     7501 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7561 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7621 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7681 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7741 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7801 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7861 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7921 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7981 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8041 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8101 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8161 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8221 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8281 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8341 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8401 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8461 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8521 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8581 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat ctcccgatcc
     8641 cctagggtcg actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     8701 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     8761 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     8821 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     8881 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     8941 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     9001 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     9061 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     9121 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     9181 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     9241 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     9301 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     9361 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     9421 tataagcaga gctggtttag tgaaccgtca gatccgctag agatccgcgg ccgctaatac
     9481 gactcactat agggagagcc gccaccatga aacggacagc cgacggaagc gagttcgagt
     9541 caccaaagaa gaagcggaaa gtcatggccg gagcacagga ttttgtccca catactgccg
     9601 atctggctga gctggctgcc gccgctggag agtgtagagg atgcgggctg taccgggatg
     9661 ccacacaggc cgtgttcgga gcaggcggca ggagcgcccg catcatgatg atcggcgagc
     9721 agccaggcga taaggaggac ctggccggcc tgccatttgt gggaccagca ggccggctgc
     9781 tggacagagc cctggaggcc gccgacatcg atagggacgc cctgtacgtg accaacgccg
     9841 tgaagcactt caagtttaca cgcgccgcag gaggcaagag gagaatccac aagaccccct
     9901 ctcggacaga ggtggtggcc tgcagacctt ggctgatcgc cgagatgacc agcgtggagc
     9961 cagatgtggt ggtgctgctg ggagcaacag cagcaaaggc cctgctgggc aatgacttca
    10021 gggtgaccca gcacagggga gaggtgctgc acgtggacga tgtgccaggc gatcctgccc
    10081 tggtggcaac cgtgcaccct agctccctgc tgagaggccc aaaggaggag cgggaatccg
    10141 ccttcgctgg gctggtggac gacctgagag tggctgccga tgtgagacct tctggaggat
    10201 ctagcggagg ctcctctggc agcgagacac caggaacaag cgagtcagca acaccagaga
    10261 gcagtggcgg cagcagcggg gggtcatcct cagagactgg gcctgtcgca gtggacccaa
    10321 ccctgaggag acggattgag ccccatgaat ttgaagtgtt ctttgaccca agggagctga
    10381 ggaaggagac atgcctgctg tacgagatca agtggggcac aagccacaag atctggcgcc
    10441 acagctccaa gaacaccaca aagcacgtgg aagtgaattt catcgagaag tttacctccg
    10501 agcggcactt ctgcccctct accagctgtt ccatcacatg gtttctgtct tggagccctt
    10561 gcggcgagtg ttccaaggcc atcaccgagt tcctgtctca gcaccctaac gtgaccctgg
    10621 tcatctacgt ggcccggctg tatcaccaca tggaccagca gaacaggcag ggcctgcgcg
    10681 atctggtgaa ttctggcgtg accatccaga tcatgacagc cccagagtac gactattgct
    10741 ggcggaactt cgtgaattat ccacctggca aggaggcaca ctggccaaga tacccacccc
    10801 tgtggatgaa gctgtatgca ctggagctgc acgcaggaat cctgggcctg cctccatgtc
    10861 tgaatatcct gcggagaaag cagccccagc tgacattttt caccattgct ctgcagtctt
    10921 gtcactatca gcggctgcct cctcatattc tgtgggccac aggcctgaag tctggaggat
    10981 ctagcggagg atcctctggc agcgagacac caggaacaag cgagtcagca acaccagaga
    11041 gcagtggcgg cagcagcggc ggcagcatgg ccggagcaca ggattttgtc ccacatactg
    11101 ccgatc
//
