LOCUS       sequence-32036-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Dreosti E, Odermatt B, Dorostkar MM, Lagnado L
  TITLE     A genetically encoded reporter of synaptic activity in vivo.
  JOURNAL   Nat Methods. 2009 Dec . 6(12):883-9.
  PUBMED    19898484
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Dec . 6(12):883-9."
COMMENT     Sequence Label: CMV::ratSyGCaMP2
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(78..99)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(339..358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             474..911
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA"
ORIGIN
        1 acgagctgta caagggcggt accggaggga gcatggtgag caagggcgag gagctgttca
       61 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      121 tgtccggcga gggtgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      181 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      241 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      301 ccgaaggcta catccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      361 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      421 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacacg cgtgaccaac
      481 tgactgaaga gcagatcgca gaatttaaag aggctttctc cctatttgac aaggacgggg
      541 atgggacaat aacaaccaag gagctgggga cggtgatgcg gtctctgggg cagaacccca
      601 cagaagcaga gctgcaggac atgatcaatg aagtagatgc cgacggtaat ggcacaatcg
      661 acttccctga gttcctgaca atgatggcaa gaaaaatgaa agacacagac agtgaagaag
      721 aaattagaga agcgttccgt gtgtttgata aggatggcaa tggctacatc agtgcagcag
      781 agcttcgcca cgtgatgaca aaccttggag agaagttaac agatgaagag gttgatgaaa
      841 tgatcaggga agcagacatc gatggggatg gtcaggtaaa ctacgaagag tttgtacaaa
      901 tgatgacagc gaa
//
