LOCUS       sequence-320360-       10305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian CG-to-GC base editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, 
            Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, 
            Adamson B, Liu DR
  TITLE     Efficient C*G-to-G*C base editors developed using CRISPRi screens, 
            target-library analysis, and machine learning.
  JOURNAL   Nat Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z.
  PUBMED    34183861
REFERENCE   2  (bases 1 to 10305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z."
COMMENT     Sequence Label: pCMV_RBMX-eA3A-UdgX-NG-nCas9
FEATURES             Location/Qualifiers
     source          1..10305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     246..265
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        246..264
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             314..334
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3017..7117
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7160..7180
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7189..7206
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7229..7246)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7235..7459
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7530..7546)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7530..7546)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7543..7565)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7554..7570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7578..7584,7585..7602,7603..7608))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7623..7644
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7761..7778)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7932..8520)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8012..8031)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8691..9482,9483..9551))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9314..9333
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9552..9656)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9735..9754)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9926..10305
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg
      241 gccgctaata cgactcacta tagggagagc cgccaccatg aaacggacag ccgacggaag
      301 cgagttcgag tcaccaaaga agaagcggaa agtcatggtt gaagcagatc gcccaggaaa
      361 gctcttcatt ggtgggctta atacggaaac aaatgagaaa gctcttgaag cagtatttgg
      421 caaatatgga cgaatagtgg aagtactctt gatgaaagac cgtgaaacca acaaatcaag
      481 aggatttgct tttgtcacct ttgaaagccc agcagacgct aaggatgcag ccagagacat
      541 gaatggaaag tcattagatg gaaaagccat caaggtggaa caagccacca aaccatcatt
      601 tgaaagtggt agacgtggac cgcctccacc tccaagaagt agaggccctc caagaggtct
      661 tagaggtgga agaggaggaa gtggaggaac caggggacct ccctcacggg gaggacacat
      721 ggatgacggt ggatattcca tgaattttaa catgagttct tccaggggac cactcccagt
      781 aaaaagagga ccaccaccaa gaagtggggg tcctcctcct aagagatctg caccttcagg
      841 accagttcgc agtagcagtg gaatgggagg aagagctcct gtatcacgtg gaagagatag
      901 ttatggaggt ccacctcgaa gggaaccgct gccctctcgt agagatgttt atttgtcccc
      961 aagagatgat gggtattcta ctaaagacag ctattcaagc agagattacc caagttctcg
     1021 tgatactaga gattatgcac caccaccacg agattatact taccgtgatt atggtcattc
     1081 cagttcacgt gatgactatc catcaagagg atatagcgat agagatggat atggtcgtga
     1141 tcgtgactat tcagatcatc caagtggagg ttcctacaga gattcatatg agagttatgg
     1201 taactcacgt agtgctccac ctacacgagg gcccccgcca tcttatggtg gaagcagtcg
     1261 ctatgatgat tacagcagct cacgtgacgg atatggtgga agtcgagaca gttactcaag
     1321 cagccgaagt gatctctact caagtggtcg tgatcgggtt ggcagacaag aaagagggct
     1381 tcccccttct atggaaaggg ggtaccctcc tccacgtgat tcctacagca gttcaagccg
     1441 cggagcacca agaggtggtg gccgtggagg aagccgatct gatagagggg gaggcagaag
     1501 cagatactct ggaggatcta gcggaggctc ctctggctct gagacacctg gcacaagcga
     1561 gagcgcaaca cctgaaagca gcgggggcag cagcgggggg tcagaggcaa gcccagcaag
     1621 cggcccacgg cacctgatgg acccccacat tttcaccagc aacttcaaca acggaatcgg
     1681 acgacacaag acctacctgt gctatgaggt ggagaggctg gacaacggca catccgtgaa
     1741 gatggatcag cacagaggct tcctgcacgg ccaggccaag aatctgctgt gcggcttcta
     1801 cggccggcac gcagagctgc gctttctgga cctggtgcca tctctgcagc tggatcccgc
     1861 ccagatctat agggtgacct ggttcatcag ctggtcccct tgctttagct ggggatgtgc
     1921 aggagaggtg agggccttcc tgcaggagaa cacacacgtg aggctgagaa tctttgccgc
     1981 ccggatctac gactatgatc ccctgtacaa ggaggccctg cagatgctga gggacgcagg
     2041 agcccaggtg tctatcatga cctatgatga gttcaagcac tgctgggaca catttgtgga
     2101 tcaccagggc tgtccctttc agccttggga cggcctggac gagcattccc aggcactgag
     2161 cggacggctg agggcaattc tgcagaacca gggcaattct ggcggatcta gcggaggatc
     2221 ctctggcagc gagacaccag gaacaagcga gtcagcaaca ccagagagca gtggcggcag
     2281 cagcggcggc agcatggccg gagcacagga ttttgtccca catactgccg atctggctga
     2341 gctggctgcc gccgctggag agtgtagagg atgcgggctg taccgggatg ccacacaggc
     2401 cgtgttcgga gcaggcggca ggagcgcccg catcatgatg atcggcgagc agccaggcga
     2461 taaggaggac ctggccggcc tgccatttgt gggaccagca ggccggctgc tggacagagc
     2521 cctggaggcc gccgacatcg atagggacgc cctgtacgtg accaacgccg tgaagcactt
     2581 caagtttaca cgcgccgcag gaggcaagag gagaatccac aagaccccct ctcggacaga
     2641 ggtggtggcc tgcagacctt ggctgatcgc cgagatgacc agcgtggagc cagatgtggt
     2701 ggtgctgctg ggagcaacag cagcaaaggc cctgctgggc aatgacttca gggtgaccca
     2761 gcacagggga gaggtgctgc acgtggacga tgtgccaggc gatcctgccc tggtggcaac
     2821 cgtgcaccct agctccctgc tgagaggccc aaaggaggag cgggaatccg ccttcgctgg
     2881 gctggtggac gacctgagag tggctgccga tgtgagacct agcggtggta gttctggcgg
     2941 tagctcagga tctgaaactc ccggcacatc tgaaagcgcg actcccgaat catctggtgg
     3001 atcttcaggt ggaagtgaca agaagtacag catcggcctg gccatcggca ccaactctgt
     3061 gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg
     3121 caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg
     3181 cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa
     3241 gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag
     3301 cttcttccac agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca
     3361 ccccatcttc ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta
     3421 ccacctgaga aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct
     3481 ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc
     3541 cgacaacagc gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt
     3601 cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact
     3661 gagcaagagc agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg
     3721 cctgttcgga aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt
     3781 cgacctggcc gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga
     3841 caacctgctg gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct
     3901 gtccgacgcc atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc
     3961 cctgagcgcc tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa
     4021 agctctcgtg cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa
     4081 gaacggctac gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat
     4141 caagcccatc ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga
     4201 ggacctgctg cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct
     4261 gggagagctg cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa
     4321 ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc
     4381 caggggaaac agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg
     4441 gaacttcgag gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac
     4501 caacttcgat aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga
     4561 gtacttcacc gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa
     4621 gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa
     4681 ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga
     4741 ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga
     4801 tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct
     4861 ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct
     4921 gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata
     4981 caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg
     5041 caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct
     5101 gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca
     5161 gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg
     5221 catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc
     5281 cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa
     5341 cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct
     5401 gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct
     5461 gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta
     5521 cgatgtggac catatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt
     5581 gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt
     5641 gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa
     5701 gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt
     5761 catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga
     5821 ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat
     5881 caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg
     5941 cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc
     6001 cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta
     6061 cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta
     6121 cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga
     6181 gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa
     6241 gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa
     6301 aaagaccgag gtgcagacag gcggcttcag caaagagtct atcaggccca agaggaacag
     6361 cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgtcag
     6421 ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa
     6481 actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa
     6541 gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat
     6601 caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc
     6661 tgccagattc ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct
     6721 gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca
     6781 gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt
     6841 ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa
     6901 gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac
     6961 caatctggga gcccctaggg ccttcaagta ctttgacacc accatcgacc ggaaggtgta
     7021 caggagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta
     7081 cgagacacgg atcgacctgt ctcagctggg aggtgactct ggcggctcaa aaagaaccgc
     7141 cgacggcagc gaattcgagc ccaagaagaa gaggaaagtc taaccggtca tcatcaccat
     7201 caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     7261 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     7321 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     7381 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     7441 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gataccgtcg
     7501 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7561 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctagggtgcc
     7621 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7681 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cgggaagagg cggtttgcgt
     7741 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7801 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7861 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7921 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7981 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8041 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8101 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8161 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8221 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8281 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8341 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8401 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8461 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8521 gaagatcctt tgatcttttc tacggggtct gacactcagt ggaacgaaaa ctcacgttaa
     8581 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8641 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8701 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8761 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8821 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8881 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8941 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9001 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9061 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9121 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9181 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9241 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9301 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9361 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9421 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9481 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9541 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9601 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9661 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
     9721 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     9781 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     9841 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     9901 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     9961 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
    10021 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
    10081 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
    10141 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
    10201 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
    10261 cctacttggc agtacatcta cgtattagtc atcgctatta ccatg
//
