LOCUS       sequence-32037-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Empty backbone for bacterial expression under T7 promoter. Uses 
            infusion based cloning method..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  TITLE     Cloning Vectors
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET15-MHL
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(124..157)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        172..617
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 ttccctctga atattttgtt tactttaaga aggagatata ccatgcatca tcatcatcat
       61 cacagcagcg gcagagaaaa cttgtatttc cagggccata tgagttctcc tcctgaaaga
      121 tccataactt cgtatagcat acattatacg aagttatgcg gccgcgacgt ccacatatac
      181 ctgccgttca ctattattta gtgaaatgag atattatgat attttctgaa ttgtgattaa
      241 aaaggcaact ttatgcccat gcaacagaaa ctataaaaaa tacagagaat gaaaagaaac
      301 agatagattt tttagttctt taggcccgta gtctgcaaat ccttttatga ttttctatca
      361 aacaaaagag gaaaatagac cagttgcaat ccaaacgaga gtctaataga atgaggtcga
      421 aaagtaaatc gcgcgggttt gttactgata aagcaggcaa gacctaaaat gtgtaaaggg
      481 caaagtgtat actttggcgt caccccttac atattttagg tcttttttta ttgtgcgtaa
      541 ctaacttgcc atcttcaaac aggagggctg gaagaagcag accgctaaca cagtacataa
      601 aaaaggagac atgaacgatg aacatcaaaa agtttgcaaa acaagcaaca gtattaacct
      661 ttactaccgc actgctggca ggaggcgcaa ctcaagcgtt tgcgaaagaa acgaaccaaa
      721 agccatataa ggaaacatac ggcatttccc atattacacg ccatgatatg ctgcaaatcc
      781 ctgaacagca aaaaaatgaa
//
