LOCUS       sequence-320372-       11106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian CG-to-GC base editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, 
            Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, 
            Adamson B, Liu DR
  TITLE     Efficient C*G-to-G*C base editors developed using CRISPRi screens, 
            target-library analysis, and machine learning.
  JOURNAL   Nat Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z.
  PUBMED    34183861
REFERENCE   2  (bases 1 to 11106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z."
COMMENT     Sequence Label: pCMV_UdgX-Anc689-UdgX-NG-nCas9-RBMX
FEATURES             Location/Qualifiers
     source          1..11106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             445..465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2692..6792
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8092..8112
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8121..8138
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8161..8178)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8167..8391
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8462..8478)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8462..8478)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8475..8497)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8486..8502
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8510..8516,8517..8534,8535..8540))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8555..8576
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8693..8710)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8864..9452)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8944..8963)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9623..10414,10415..10483))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10246..10265
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10484..10588)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10667..10686)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(10858..11106,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcatggc cggagcacag
      481 gattttgtcc cacatactgc cgatctggct gagctggctg ccgccgctgg agagtgtaga
      541 ggatgcgggc tgtaccggga tgccacacag gccgtgttcg gagcaggcgg caggagcgcc
      601 cgcatcatga tgatcggcga gcagccaggc gataaggagg acctggccgg cctgccattt
      661 gtgggaccag caggccggct gctggacaga gccctggagg ccgccgacat cgatagggac
      721 gccctgtacg tgaccaacgc cgtgaagcac ttcaagttta cacgcgccgc aggaggcaag
      781 aggagaatcc acaagacccc ctctcggaca gaggtggtgg cctgcagacc ttggctgatc
      841 gccgagatga ccagcgtgga gccagatgtg gtggtgctgc tgggagcaac agcagcaaag
      901 gccctgctgg gcaatgactt cagggtgacc cagcacaggg gagaggtgct gcacgtggac
      961 gatgtgccag gcgatcctgc cctggtggca accgtgcacc ctagctccct gctgagaggc
     1021 ccaaaggagg agcgggaatc cgccttcgct gggctggtgg acgacctgag agtggctgcc
     1081 gatgtgagac cttctggagg atctagcgga ggctcctctg gcagcgagac accaggaaca
     1141 agcgagtcag caacaccaga gagcagtggc ggcagcagcg gggggtcatc ctcagagact
     1201 gggcctgtcg cagtggaccc aaccctgagg agacggattg agccccatga atttgaagtg
     1261 ttctttgacc caagggagct gaggaaggag acatgcctgc tgtacgagat caagtggggc
     1321 acaagccaca agatctggcg ccacagctcc aagaacacca caaagcacgt ggaagtgaat
     1381 ttcatcgaga agtttacctc cgagcggcac ttctgcccct ctaccagctg ttccatcaca
     1441 tggtttctgt cttggagccc ttgcggcgag tgttccaagg ccatcaccga gttcctgtct
     1501 cagcacccta acgtgaccct ggtcatctac gtggcccggc tgtatcacca catggaccag
     1561 cagaacaggc agggcctgcg cgatctggtg aattctggcg tgaccatcca gatcatgaca
     1621 gccccagagt acgactattg ctggcggaac ttcgtgaatt atccacctgg caaggaggca
     1681 cactggccaa gatacccacc cctgtggatg aagctgtatg cactggagct gcacgcagga
     1741 atcctgggcc tgcctccatg tctgaatatc ctgcggagaa agcagcccca gctgacattt
     1801 ttcaccattg ctctgcagtc ttgtcactat cagcggctgc ctcctcatat tctgtgggcc
     1861 acaggcctga agtctggagg atctagcgga ggatcctctg gcagcgagac accaggaaca
     1921 agcgagtcag caacaccaga gagcagtggc ggcagcagcg gcggcagcat ggccggagca
     1981 caggattttg tcccacatac tgccgatctg gctgagctgg ctgccgccgc tggagagtgt
     2041 agaggatgcg ggctgtaccg ggatgccaca caggccgtgt tcggagcagg cggcaggagc
     2101 gcccgcatca tgatgatcgg cgagcagcca ggcgataagg aggacctggc cggcctgcca
     2161 tttgtgggac cagcaggccg gctgctggac agagccctgg aggccgccga catcgatagg
     2221 gacgccctgt acgtgaccaa cgccgtgaag cacttcaagt ttacacgcgc cgcaggaggc
     2281 aagaggagaa tccacaagac cccctctcgg acagaggtgg tggcctgcag accttggctg
     2341 atcgccgaga tgaccagcgt ggagccagat gtggtggtgc tgctgggagc aacagcagca
     2401 aaggccctgc tgggcaatga cttcagggtg acccagcaca ggggagaggt gctgcacgtg
     2461 gacgatgtgc caggcgatcc tgccctggtg gcaaccgtgc accctagctc cctgctgaga
     2521 ggcccaaagg aggagcggga atccgccttc gctgggctgg tggacgacct gagagtggct
     2581 gccgatgtga gacctagcgg tggtagttct ggcggtagct caggatctga aactcccggc
     2641 acatctgaaa gcgcgactcc cgaatcatct ggtggatctt caggtggaag tgacaagaag
     2701 tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag
     2761 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
     2821 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
     2881 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
     2941 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc
     3001 ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac
     3061 gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac
     3121 agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc
     3181 cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg
     3241 ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc
     3301 ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat
     3361 ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg
     3421 agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg
     3481 cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac
     3541 cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac
     3601 atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga
     3661 tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct
     3721 gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac
     3781 ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac
     3841 ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc
     3901 ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg
     3961 cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg
     4021 accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg
     4081 atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag
     4141 ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac
     4201 gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg
     4261 accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag
     4321 aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg
     4381 aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa
     4441 gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag
     4501 gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca
     4561 ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac
     4621 gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg
     4681 aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag
     4741 tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt
     4801 aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt
     4861 gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg
     4921 gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc
     4981 agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc
     5041 gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc
     5101 cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg
     5161 gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag
     5221 agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg
     5281 ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg
     5341 cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag
     5401 agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc
     5461 cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac
     5521 gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc
     5581 gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc
     5641 cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg
     5701 gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag
     5761 agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac
     5821 tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag
     5881 acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg
     5941 aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc
     6001 ttcagcaaag agtctatcag gcccaagagg aacagcgata agctgatcgc cagaaagaag
     6061 gactgggacc ctaagaagta cggcggcttc gtcagcccca ccgtggccta ttctgtgctg
     6121 gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg
     6181 gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc
     6241 aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc
     6301 gagctggaaa acggccggaa gagaatgctg gcctctgcca gattcctgca gaagggaaac
     6361 gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag
     6421 ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac
     6481 tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac
     6541 gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag
     6601 caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc tagggccttc
     6661 aagtactttg acaccaccat cgaccggaag gtgtacagga gcaccaaaga ggtgctggac
     6721 gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag
     6781 ctgggaggtg actctggagg atctagcgga ggatcctctg gcagcgagac accaggaaca
     6841 agcgagtcag caacaccaga gagcagtggc ggcagcagcg gcggcagcat ggttgaagca
     6901 gatcgcccag gaaagctctt cattggtggg cttaatacgg aaacaaatga gaaagctctt
     6961 gaagcagtat ttggcaaata tggacgaata gtggaagtac tcttgatgaa agaccgtgaa
     7021 accaacaaat caagaggatt tgcttttgtc acctttgaaa gcccagcaga cgctaaggat
     7081 gcagccagag acatgaatgg aaagtcatta gatggaaaag ccatcaaggt ggaacaagcc
     7141 accaaaccat catttgaaag tggtagacgt ggaccgcctc cacctccaag aagtagaggc
     7201 cctccaagag gtcttagagg tggaagagga ggaagtggag gaaccagggg acctccctca
     7261 cggggaggac acatggatga cggtggatat tccatgaatt ttaacatgag ttcttccagg
     7321 ggaccactcc cagtaaaaag aggaccacca ccaagaagtg ggggtcctcc tcctaagaga
     7381 tctgcacctt caggaccagt tcgcagtagc agtggaatgg gaggaagagc tcctgtatca
     7441 cgtggaagag atagttatgg aggtccacct cgaagggaac cgctgccctc tcgtagagat
     7501 gtttatttgt ccccaagaga tgatgggtat tctactaaag acagctattc aagcagagat
     7561 tacccaagtt ctcgtgatac tagagattat gcaccaccac cacgagatta tacttaccgt
     7621 gattatggtc attccagttc acgtgatgac tatccatcaa gaggatatag cgatagagat
     7681 ggatatggtc gtgatcgtga ctattcagat catccaagtg gaggttccta cagagattca
     7741 tatgagagtt atggtaactc acgtagtgct ccacctacac gagggccccc gccatcttat
     7801 ggtggaagca gtcgctatga tgattacagc agctcacgtg acggatatgg tggaagtcga
     7861 gacagttact caagcagccg aagtgatctc tactcaagtg gtcgtgatcg ggttggcaga
     7921 caagaaagag ggcttccccc ttctatggaa agggggtacc ctcctccacg tgattcctac
     7981 agcagttcaa gccgcggagc accaagaggt ggtggccgtg gaggaagccg atctgataga
     8041 gggggaggca gaagcagata caaaagaacc gccgacggca gcgaattcga gcccaagaag
     8101 aagaggaaag tctaaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag
     8161 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     8221 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     8281 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     8341 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     8401 cggaaagaac cagctggggc tcgataccgt cgacctctag ctagagcttg gcgtaatcat
     8461 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8521 ccggaagcat aaagtgtaaa gcctagggtg cctaatgagt gagctaactc acattaattg
     8581 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8641 tcggccaacg cgcgggaaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8701 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8761 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8821 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8881 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8941 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9001 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9061 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9121 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9181 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9241 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9301 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9361 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9421 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9481 ctgacactca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9541 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9601 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9661 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9721 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9781 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9841 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9901 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9961 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10021 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10081 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10141 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10201 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10261 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10321 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10381 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10441 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10501 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10561 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    10621 acggatcggg agatcgatct cccgatcccc tagggtcgac tctcagtaca atctgctctg
    10681 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
    10741 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
    10801 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
    10861 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
    10921 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
    10981 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
    11041 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
    11101 tgtatc
//
