LOCUS       sequence-32038-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Chivers CE, Crozat E, Chu C, Moy VT, Sherratt DJ, Howarth M
  TITLE     A streptavidin variant with slower biotin dissociation and increased
            mechanostability.
  JOURNAL   Nat Methods. 2010 May;7(5):391-3. doi: 10.1038/nmeth.1450. Epub 2010
            Apr 11.
  PUBMED    20383133
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1450"; journalName: "Nat Methods"; date: "2010-05"; 
            volume: "7"; issue: "5"; pages: "391-3"
COMMENT     Sequence Label: pET21a-Core Traptavidin
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             461..478
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(531..549)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      545..592
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(716..735)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgggaattcc ctctgaatat tttgtttact ttaagaagga gatatacata tggctgaagc
       61 tggtatcacc ggcacctggt acaaccagct gggatccacc ttcatcgtta ccgctggtgc
      121 tgacggtgct ctgaccggta cctacgaatc cgctgttggt aacgctgaag gcgattacgt
      181 tctgaccggt cgttacgact ccgctccggc taccgacggt tccggaaccg ctctgggttg
      241 gaccgttgct tggaaaaaca actaccgtaa cgctcactcc gctaccacct ggtctggcca
      301 gtacgttggt ggtgctgaag ctcgtatcaa cacccagtgg ttgttgacct ccggcaccac
      361 cgaagctaac gcgtggaaat ccaccctggt tggtcacgac accttcacca aagttaaacc
      421 gtccgctgct tcctaataaa agcttgcggc cgcactcgag caccaccacc accaccactg
      481 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      541 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
      601 aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
      661 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
      721 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
      781 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
      841 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
      901 agtg
//
