LOCUS       sequence-32040-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   McNutt MC, Kwon HJ, Chen C, Chen JR, Horton JD, Lagace TA
  TITLE     Antagonism of secreted PCSK9 increases low density lipoprotein 
            receptor expression in HepG2 cells.
  JOURNAL   J Biol Chem. 2009 Apr 17. 284(16):10561-70.
  PUBMED    19224862
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2009 Apr 17. 284(16):10561-70."
COMMENT     Sequence Label: pGEX-EGFAB(H306Y)
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(316..338)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(482..500)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        568..672
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcgggcgc catggggacc aacgaatgct tggacaacaa cggcggctgt tcctacgtct
       61 gcaatgacct taagatcggc tacgagtgcc tgtgccccga cggcttccag ctggtggccc
      121 agcgaagatg cgaagatatc gatgagtgtc aggatcccga cacctgcagc cagctctgcg
      181 tgaacctgga gggtggctac aagtgccagt gtgaggaagg cttccagctg gacccccaca
      241 cgaaggcctg caagtaagaa ttcatcgtga ctgactgacg atctgcctcg cgcgtttcgg
      301 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      361 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      421 gggcgcagcc atgacccagt cacgtagcga tagcggagtg taaattcttg aagacgaaag
      481 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
      541 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      601 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      661 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      721 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
      781 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatcc
//
