LOCUS       sequence-32041-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Kwon HJ, Abi-Mosleh L, Wang ML, Deisenhofer J, Goldstein JL, Brown 
            MS, Infante RE
  TITLE     Structure of N-terminal domain of NPC1 reveals distinct subdomains 
            for binding and transfer of cholesterol.
  JOURNAL   Cell. 2009 Jun 26. 137(7):1213-24.
  PUBMED    19563754
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jun 26. 137(7):1213-24."
COMMENT     Sequence Label: pFAST NPC1(NTD)-3Q
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     90..152
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
     CDS             165..182
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             204..224
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gtttcgtaca gttttgtata aaaaaaccta taaatattcc ggattattca taccgtccca
       61 ccatcgggcg cggatctcgg tccgaaacca tgaaattctt agtcaacgtt gcccttgttt
      121 ttatggtcgt atacatttct tacatctatg cggattcgta ctaccatcac catcaccatc
      181 acgattacga tatcccaacg accgaaaacc tgtattttca gggcgcccag tcctgtgttt
      241 ggtatggaga gtgtggaatt gcatatgggg acaagaggta caattgcgaa tattctggcc
      301 caccaaaacc attgccaaag gatggatatg acttagtgca ggaactctgt ccaggattct
      361 tctttggcca ggtcagtctc tgttgtgatg ttcggcagct tcagacacta aaagacaacc
      421 tgcagctgcc tctacagttt ctgtccagat gtccatcctg tttttataac ctactgaacc
      481 tgttttgtga gctgacatgt agccctcgac agagtcagtt tttgcaggtt acagctactg
      541 aagattatgt tgatcctgtt acaaaccaga cgaaaacaaa tgtgaaagag ttacaatact
      601 acgtcggaca gagttttgcc aatgcaatgt acaatgcctg ccgggatgtg gaggccccct
      661 caagtaatga caaggccctg ggactcctgt gtgggaagga cgctgacgcc tgtcaggcca
      721 ccaactggat tgaatacatg ttcaataagg acaatggaca ggcacctttt accatcactc
      781 ctgtgttttc agattttcca gtccatggga tggagcccat gaacaatgcc accaaaggct
      841 gtgacgagtc tgtggatgag gtcacagcac catgta
//
