LOCUS       sequence-320422-         567 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP cDNA and KRAS G12V cDNA in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Carver J, Dexheimer TS, Hsu D, Weng MT, Smith JL, Guha R, Jadhav A, 
            Simeonov A, Luo J
  TITLE     A high-throughput assay for small molecule destabilizers of the KRAS
            oncoprotein.
  JOURNAL   PLoS One. 2014 Aug 5;9(8):e103836. doi: 
            10.1371/journal.pone.0103836. eCollection 2014.
  PUBMED    25093678
REFERENCE   2  (bases 1 to 567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-08-5"; volume: "9(8):e103836. doi"; pages: " 
            10.1371/journal.pone.0103836. eCollection 2014"
COMMENT     Sequence Label: pInducer10b-EGFP-KRAS G12V
FEATURES             Location/Qualifiers
     source          1..567
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..33,34..36,37..567)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
ORIGIN
        1 atgactgaat ataaacttgt ggtagttgga gctgttggcg taggcaagag tgccttgacg
       61 atacagctaa ttcagaatca ttttgtggac gaatatgatc caacaataga ggattcctac
      121 aggaagcaag tagtaattga tggagaaacc tgtctcttgg atattctcga cacagcaggt
      181 caagaggagt acagtgcaat gagggaccag tacatgagga ctggggaggg ctttctttgt
      241 gtatttgcca taaataatac taaatcattt gaagatattc accattatag agaacaaatt
      301 aaaagagtta aggactctga agatgtacct atggtcctag taggaaataa atgtgatttg
      361 ccttctagaa cagtagacac aaaacaggct caggacttag caagaagtta tggaattcct
      421 tttattgaaa catcagcaaa gacaagacag ggtgttgatg atgccttcta tacattagtt
      481 cgagaaattc gaaaacataa agaaaagatg agcaaagatg gtaaaaagaa gaaaaagaag
      541 tcaaagacaa agtgtgtaat tatgtaa
//
