LOCUS       sequence-320454-        9957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination vector with bidirectional CMV-SFFV promoter for 
            high expression of gene of interest and GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   van Galen P, Kreso A, Wienholds E, Laurenti E, Eppert K, Lechman ER,
            Mbong N, Hermans K, Dobson S, April C, Fan JB, Dick JE
  TITLE     Reduced lymphoid lineage priming promotes human hematopoietic stem 
            cell expansion.
  JOURNAL   Cell Stem Cell. 2014 Jan 2;14(1):94-106. doi: 
            10.1016/j.stem.2013.11.021.
  PUBMED    24388174
REFERENCE   2  (bases 1 to 9957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.11"; journalName: "Cell Stem Cell"; date: 
            "2014-01-2- 2"; volume: "14"; issue: "1"; pages: "94-106"
COMMENT     Sequence Label: pSMAL
FEATURES             Location/Qualifiers
     source          1..9957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      112..247
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(267..286)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(268..286)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(296..313)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(296..312)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(305..327)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      454..909
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(541..560)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     751..772
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        935..1039
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1040..1108,1109..1900)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1258..1277)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2071..2659
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2560..2579
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2813..2830
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2947..2968
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2983..2988,2989..3006,3007..3013)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3021..3037
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3026..3048
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3045..3061
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3045..3061
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3080..3100
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3082..3100
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        3201..3580
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3581..3784
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             3799..3979
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4026..4151
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4644..4877
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5062..5106
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5373..5490
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    5622..5756
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(5799..6512,6513..6515,6516..6518))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5802..6512,6513..6515,6516..6518))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5844..5865)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6193..6212
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6452..6473
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(6622..6660)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    complement(7094..7127)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    7142..7266
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7291..7296,7297..7314,7315..7321)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7375..8034
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKRNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7441..7460)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8376..8681
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8560..8579
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8722..8846)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8872..8888)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8872..8888)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(8889..8922)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    8928..9516
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8981..9001)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9399..9410)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9603..9836
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    join(9908..9957,1..72)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
       61 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
      121 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
      181 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
      241 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
      301 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      361 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      421 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
      481 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      541 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      601 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      661 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      721 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
      781 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
      841 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
      901 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      961 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1021 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     1081 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     1141 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     1201 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     1261 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     1321 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     1381 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     1441 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1501 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1561 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1621 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1681 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     1741 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     1801 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1861 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1921 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1981 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2041 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2101 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2161 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2221 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2281 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2341 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2401 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2461 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2521 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2581 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2641 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2701 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2761 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2821 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2881 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2941 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     3001 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     3061 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     3121 agcttggcca ttgcatacgt tgtatccata tcataatatg tacatttata ttggctcatg
     3181 tccaacatta ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
     3241 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     3301 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     3361 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     3421 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     3481 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     3541 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     3601 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     3661 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     3721 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     3781 agctcgttta gtgaaccggg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     3841 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     3901 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     3961 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     4021 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     4081 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     4141 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     4201 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     4261 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     4321 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     4381 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     4441 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     4501 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     4561 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     4621 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     4681 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     4741 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     4801 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     4861 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     4921 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     4981 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     5041 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     5101 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     5161 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     5221 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     5281 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     5341 ttagtgaacg gatctcgacg gtatcggtta acttttaaaa gaaaaggggg gattgggggg
     5401 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     5461 caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg agactagcct cgagtgatca
     5521 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     5581 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     5641 ataatggtta caaataaggc aatagcatca caaatttcac aaataaggca tttttttcac
     5701 tgcattctag ttttggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctcaa
     5761 atccctcgga agctgcgcct gtcatcgaat tcaccggtct acttgtacag ctcgtccatg
     5821 ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg
     5881 ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg
     5941 gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc
     6001 tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg
     6061 atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc
     6121 tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc
     6181 acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg
     6241 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
     6301 ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc
     6361 ttgccggtgg tgcagatgaa cttcagggtc agcttaccgt aggtggcatc gccctcgccc
     6421 tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
     6481 accaccccgg tgaacagctc ctcgcccttg ctcaccatgg tggtaccgaa ttccgcggag
     6541 gctggatcgg tcccggtgtc ttctatggag gtcaaaacag cgtggatggc gtctccaggc
     6601 gatctgacgg ttcactaaac gagctctgct tatataggcc tcccaccgta cacgcctacc
     6661 actagtaatg aaagacccca cctgtaggtt tggcaagcta gctgcagtaa cgccattttg
     6721 caaggcatgg aaaaatacca aaccaagaat agagaagttc agatcaaggg cgggtacatg
     6781 aaaatagcta acgttgggcc aaacaggata tctgcggtga gcagtttcgg ccccggcccg
     6841 gggccaagaa cagatggtca ccgcagtttc ggccccggcc cgaggccaag aacagatggt
     6901 ccccagatat ggcccaaccc tcagcagttt cttaagaccc atcagatgtt tccaggctcc
     6961 cccaaggacc tgaaatgacc ctgcgcctta tttgaattaa ccaatcagcc tgcttctcgc
     7021 ttctgttcgc gcgcttctgc ttcccgagct ctataaaaga gctcacaacc cctcactcgg
     7081 cttaattaag atcataactt cgtatagcat acattatacg aagttatccc agcttgatat
     7141 cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
     7201 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
     7261 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat
     7321 gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag
     7381 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     7441 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
     7501 gcctttttaa agaccgtaaa gagaaataag cacaagtttt atccggcctt tattcacatt
     7561 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg
     7621 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     7681 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     7741 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     7801 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     7861 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     7921 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     7981 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaaacgcgt
     8041 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt
     8101 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc
     8161 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt
     8221 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga
     8281 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa
     8341 cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata
     8401 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg
     8461 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg
     8521 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg
     8581 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg
     8641 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac
     8701 acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta
     8761 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
     8821 cgttcagctt tcttgtacaa agtggtgata tcgaattcct gcagcccggg ggatccacta
     8881 gttctagaat aacttcgtat agcatacatt atacgaagtt attcgacaat caacctctgg
     8941 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     9001 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     9061 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     9121 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     9181 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     9241 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     9301 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     9361 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     9421 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     9481 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtaccttt
     9541 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     9601 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     9661 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     9721 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     9781 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     9841 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     9901 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcac
//
