LOCUS       sequence-32046-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Carlson HJ, Cotton DW, Campbell RE
  TITLE     Circularly permuted monomeric red fluorescent proteins with new 
            termini in the beta-sheet.
  JOURNAL   Protein Sci. 2010 Aug;19(8):1490-9. doi: 10.1002/pro.428.
  PUBMED    20521333
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro"; 
            journalName: "Protein Sci"; date: "2010-08"; volume: "19"; issue: 
            "8"; pages: "1490-9"
COMMENT     Sequence Label: cp193g7
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             77..94
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             98..130
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(134..142,143..157)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(456..474)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     724..743
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tttttatcgc aactctctac tgtttctcca tacccgtttt ttgggctaac aggaggaatt
       61 aaccatgggg ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca
      121 gcaaatgggt cgggatctgt acgacgatga cgataaggat ccgagctcga gggcctacaa
      181 cgtcgacatc aagttggaca tcacctcaca caacgaggac tacaccatcg tggaacagta
      241 cgaacgcgcc gaggtccgcc actccaccgg cggcatggac gagctgtaca agggcggtac
      301 cggcggctcc atggtgagca agggcgtgga ggataacatg gccttcatca aggagttcat
      361 gcgcttcaag gtgcacatgg agggctccgt caacggccac gagttcgaga tcgagggcga
      421 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
      481 ccccctgccc ttcgcctggg acatcctgtc ccctcagctc atgtacggct ccaaggccta
      541 cgtgaagcac cccgccgaca tccccgacta ctggaagctg tccttccccg agggcttcaa
      601 gtgggagcgc gtgatgaact tcgaggacgg aggcgtggtg accgtgaccc aggactcctc
      661 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
      721 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
      781 cgaggacggc gctctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
      841 ctacgacgct gaggtcaaga ccacctaca
//
