LOCUS       sequence-32047-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Carlson HJ, Cotton DW, Campbell RE
  TITLE     Circularly permuted monomeric red fluorescent proteins with new 
            termini in the beta-sheet.
  JOURNAL   Protein Sci. 2010 Aug;19(8):1490-9. doi: 10.1002/pro.428.
  PUBMED    20521333
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro"; 
            journalName: "Protein Sci"; date: "2010-08"; volume: "19"; issue: 
            "8"; pages: "1490-9"
COMMENT     Sequence Label: cp204
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..96
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             100..132
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(136..144,145..159)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(449..467)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     717..736
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctttttatc gcaactctct actgtttctc catacccgtt ttttgggcta acaggaggaa
       61 ttaaccatgg ggggttctca tcatcatcat catcatggta tggctagcat gactggtgga
      121 cagcaaatgg gtcgggatct gtacgacgat gacgataagg atccgagctc gagcatgtcc
      181 agctccgggg cactgggcgg tacgaacgag gactacacca tcgtggaaca gtacgaacgc
      241 gccgaggtcc gccactccac cggcggcatg gacgagctgt acaagggtgg cactggcggt
      301 tccatggtga gcaagggcgt ggaggataac atggccttca tcaaggagtt catgcgcttc
      361 aaggtgcaca tggagggctc cgtcaacggc cacgagttcg agatcgaggg cgagggcgag
      421 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
      481 cccttcgcct gggacatcct gtcccctcag ctcatgtacg gctccaaggc ctacgtgaag
      541 caccccgccg acatccccga ctactggaag ctgtccttcc ccgagggctt caagtgggag
      601 cgcgtgatga acttcgagga cggaggcgtg gtgaccgtga cccaggactc ctccctgcag
      661 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
      721 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
      781 ggcgctctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
      841 gctgaggtca agaccaccta c
//
