LOCUS       sequence-320470-        1296 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NPRL2 expressing vector for mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Lawrence RE, Fromm SA, Fu Y, Yokom AL, Kim DJ, Thelen AM, Young LN, 
            Lim CY, Samelson AJ, Hurley JH, Zoncu R
  TITLE     Structural mechanism of a Rag GTPase activation checkpoint by the 
            lysosomal folliculin complex.
  JOURNAL   Science. 2019 Nov 22;366(6468):971-977. doi: 
            10.1126/science.aax0364. Epub 2019 Oct 31.
  PUBMED    31672913
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aax0364"; journalName: "Science"; date: 
            "2019-11-22- 22"; volume: "366"; issue: "6468"; pages: "971-977"
COMMENT     Sequence Label: pCAG-TWIN-STREP-FLAG-NPRL2
FEATURES             Location/Qualifiers
     source          1..1296
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             22..45
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             82..105
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             112..135
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atcgatgcca ccatggctag ctggagccac ccgcagttcg agaaaggtgg aggtgcccga
       61 ggtggatcgg gaggtggatc gtggagccac ccgcagttcg aaaaaggatt cgattacaag
      121 gatgacgatg acaagggtac catgggctcc ggctgcagga tcgagtgtat cttcttttct
      181 gagttccacc ctaccctggg cccaaagatc acataccagg tgccagagga cttcatctcc
      241 cgcgagctgt ttgataccgt gcaggtgtat atcatcacaa agcccgagct gcagaacaag
      301 ctgatcaccg tgacagctat ggagaagaag ctgatcggct gccccgtgtg catcgagcac
      361 aagaagtact ctcggaacgc cctgctgttc aatctgggct tcgtgtgcga cgcccaggcc
      421 aagacatgtg ccctggagcc aatcgtgaag aagctggccg gctatctgac cacactggag
      481 ctggagagca gcttcgtgag catggaggag tctaagcaga agctggtgcc catcatgacc
      541 atcctgctgg aggagctgaa cgccagcggc aggtgcaccc tgcctatcga cgagtccaat
      601 acaatccacc tgaaagtgat cgagcagcgc cccgaccccc ctgtggcaca ggagtacgat
      661 gtgcccgtgt tcaccaagga caaggaggat ttctttaact ctcagtggga cctgaccaca
      721 cagcagatcc tgccctatat cgacggcttt cggcacatcc agaagatcag cgccgaggca
      781 gacgtggagc tgaacctggt gagaatcgcc atccagaatc tgctgtacta tggcgtggtg
      841 acactggtga gcatcctgca gtactccaac gtgtactgcc caacccccaa ggtgcaggac
      901 ctggtggacg ataagtctct gcaggaggcc tgtctgagct acgtgaccaa gcagggccac
      961 aagcgggcct ccctgagaga cgtgttccag ctgtattgtt ctttgagccc tggcaccaca
     1021 gtgagggacc tgatcggccg ccacccacag cagctgcagc acgtggatga gaggaagctg
     1081 atccagtttg gcctgatgaa gaatctgatc cggagactgc agaagtaccc agtgagggtg
     1141 acccgcgagg agcagtctca cccagccaga ctgtacacag gctgccacag ctatgatgag
     1201 atctgctgta agaccggcat gtcctaccac gagctggacg agagactgga gaacgatcct
     1261 aatatcatca tctgttggaa gtgactcgag gaattc
//
