LOCUS       sequence-320472-        4824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DEPDC5 expressing vector for mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Lawrence RE, Fromm SA, Fu Y, Yokom AL, Kim DJ, Thelen AM, Young LN, 
            Lim CY, Samelson AJ, Hurley JH, Zoncu R
  TITLE     Structural mechanism of a Rag GTPase activation checkpoint by the 
            lysosomal folliculin complex.
  JOURNAL   Science. 2019 Nov 22;366(6468):971-977. doi: 
            10.1126/science.aax0364. Epub 2019 Oct 31.
  PUBMED    31672913
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aax0364"; journalName: "Science"; date: 
            "2019-11-22- 22"; volume: "366"; issue: "6468"; pages: "971-977"
COMMENT     Sequence Label: pCAG GST DEPDC5
FEATURES             Location/Qualifiers
     source          1..4824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggtaccatgc ggacaacaaa ggtctacaaa ctggtcatcc ataaaaaagg gttcggcggg
       61 agcgacgacg agctggtggt gaatcctaag gtgtttcccc acatcaagct gggcgatatc
      121 gtggagatcg cccaccccaa tgacgagtat tctcccctgc tgctgcaggt gaagtctctg
      181 aaggaggatc tgcagaagga gacaatcagc gtggaccaga cagtgaccca ggtgtttcgg
      241 ctgcggccat accaggacgt gtatgtgaac gtggtggacc ccaaggatgt gacactggat
      301 ctggtggagc tgacctttaa ggatcagtac atcggcagag gcgatatgtg gcggctgaag
      361 aagtccctgg tgtccacatg cgcctacatc acccagaagg tggagttcgc cggcatcaga
      421 gcccaggccg gcgagctgtg ggtgaagaat gagaaggtaa tgtgcggcta catcagcgag
      481 gatacacggg tggtgtttcg gtctacatcc gccatggtgt atatcttcat ccagatgtcc
      541 tgcgagatgt gggacttcga catctatggc gacctgtact tcgagaaggc cgtgaatggc
      601 tttctggccg atctgtttac caagtggaag gagaagaatt gtagccacga ggtgacagtg
      661 gtgctgttta gccggacctt ctatgacgcc aagtctgtgg acgagttccc tgagatcaat
      721 agagcctcta tcagacagga tcacaagggc cggttttacg aggacttcta caaggtggtg
      781 gtgcagaatg agagacggga ggagtggaca tctctgctgg tgaccatcaa gaagctgttc
      841 atccagtacc cagtgctggt gcggctggag caggccgagg gcttccccca gggcgacaat
      901 tccaccagcg cccagggcaa ctacctggag gccatcaacc tgtcctttaa cgtgttcgat
      961 aagcactata tcaacagaaa cttcgaccgg acaggccaga tgtccgtggt aatcacaccc
     1021 ggcgtgggcg tgtttgaggt ggatagactg ctgatgatcc tgaccaagca gcgcatgatc
     1081 gacaatggca tcggcgtgga tctggtgtgc atgggcgagc agcctctgca cgccgtgccc
     1141 ctgttcaagc tgcacaacag gtccgccccc agagacagcc ggctgggcga tgattacaac
     1201 atcccacact ggatcaatca cagcttctac acctctaagt cccagctgtt ttgtaacagc
     1261 ttcacaccac ggatcaagct ggccggcaag aagcctgcca gcgagaaggc caagaacggc
     1321 agagacacca gcctgggcag ccctaaggag tccgagaacg ccctgcctat ccaggtggac
     1381 tacgatgcct acgacgccca ggtgttcagg ctgcccggcc catccagagc ccagtgtctg
     1441 acaacctgca gatctgtgag agagagagag tctcactcca gaaagtccgc ctcctcctgc
     1501 gacgtgtcct cctccccaag cctgccatcc cggaccctgc ccaccgagga ggtgagaagc
     1561 caggccagcg atgattccag cctgggcaag tctgccaata tcctgatgat cccccaccca
     1621 cacctgcacc agtatgaggt gtccagctcc ctgggctaca cctccaccag agacgtgctg
     1681 gagaatatga tggagccacc tcagcgggat tcttccgccc caggcagatt ccacgtgggc
     1741 tccgccgaga gcatgctgca cgtgcggcct ggcggctaca ccccacagag agccctgatc
     1801 aacccattcg ccccttccag aatgccaatg aagctgacaa gcaacagacg caggtggatg
     1861 cacaccttcc ctgtgggccc ctccggcgag gccatccaga tccaccacca gacccgccag
     1921 aacatggccg agctgcaggg ctccggccag cgcgacccaa cccactctag cgccgagctg
     1981 ctggagctgg cctatcacga ggccgccggc cggcacagca actccagaca gccaggcgat
     2041 ggcatgagct tcctgaactt ttccggcaca gaggagctgt ccgtgggcct gctgtccaat
     2101 agcggcgccg gcatgaaccc tcgcacccag aacaaggaca gcctggagga ttccgtgtcc
     2161 acctctcccg atccaatcct gacactgtcc gcccccccag tggtgccagg cttttgctgt
     2221 accgtgggcg tggattggaa gtccctgaca accccagcct gtctgcccct gaccaccgat
     2281 tactttcctg atagacaggg cctgcagaac gactacaccg agggctgcta tgatctgctg
     2341 ccagaggccg acatcgaccg gagggatgag gatggcgtgc agatgaccgc ccagcaggtg
     2401 ttcgaggagt tcatctgcca gagactgatg cagggctacc agatcatcgt gcagcctaag
     2461 acccagaagc ctaatccagc cgtgccaccc ccactgtctt cctccccact gtactccaga
     2521 ggcctggtgt ccagaaaccg gccagaggag gaggaccagt actggctgag catgggccgg
     2581 acctttcaca aggtgaccct gaaggataag atgatcacag tgacaagata tctgccaaag
     2641 tacccatacg agtccgccca gatccactat acctattctc tgtgccctag ccactccgat
     2701 tccgagttcg tgagctgctg ggtggagttt agccacgaga ggctggagga gtataagtgg
     2761 aactatctgg accagtatat ctgtagcgcc ggctccgagg atttctccct gatcgagtcc
     2821 ctgaagtttt ggcgcaccag atttctgctg ctgccagcct gcgtgaccgc cacaaagcgc
     2881 atcaccgagg gcgaggccca ctgcgatatc tatggcgatc ggcctagagc cgacgaggat
     2941 gagtggcagc tgctggatgg ctttgtgaga tttgtggagg gcctgaacag aatcaggcgg
     3001 cggcacaggt ccgatagaat gatgcggaag ggcaccgcca tgaagggcct gcagatgacc
     3061 ggccccatct ccacccactc cctggagtct accgcccccc cagtgggcaa gaagggcaca
     3121 agcgccctgt ctgccctgct ggagatggag gccagccaga agtgcctggg cgagcagcag
     3181 gccgccgtgc acggcggcaa gagctccgcc cagagcgccg agtccagctc tgtggccatg
     3241 acccccacat atatggactc cccacgcaag gatggcgcct tctttatgga gtttgtgcgg
     3301 tctccaagaa ccgcctcctc cgccttctat ccacaggtgt ccgtggatca gacagccacc
     3361 ccaatgctgg atggcacaag cctgggcatc tgtacaggcc agagcatgga tagaggcaac
     3421 tctcagacct tcggcaacag ccagaatatc ggcgagcagg gctattcctc cacaaactcc
     3481 tccgactcct cctcccagca gctggtggcc tccagcctga caagctcctc caccctgaca
     3541 gagatcctgg aggccatgaa gcacccatct accggcgtgc agctgctgtc tgagcagaag
     3601 ggcctgtccc catactgctt catctccgcc gaggtggtgc actggctggt gaatcacgtg
     3661 gagggcatcc agacccaggc catggccatc gacatcatgc agaagatgct ggaggagcag
     3721 ctgatcaccc acgcctctgg cgaggcctgg agaaccttta tctacggctt ttacttctac
     3781 aagatcgtga cagataagga gcccgatagg gtggccatgc agcagcctgc cacaacctgg
     3841 cacaccgccg gcgtggatga ttttgccagc tttcagcgga agtggttcga ggtggccttt
     3901 gtggccgagg agctggtgca cagcgagatc ccagcctttc tgctgccttg gctgccatct
     3961 cgcccagcct cttatgccag cagacactcc agcttctcca ggagcttcgg cggcaggagc
     4021 caggccgccg ccctgctggc cgccacagtg ccagagcaga gaaccgtgac actggacgtg
     4081 gacgtgaata acaggaccga cagactggag tggtgttcct gctactatca cggcaacttc
     4141 tccctgaatg ccgccttcga gatcaagctg cactggatgg ccgtgaccgc cgccgtgctg
     4201 ttcgagatgg tgcagggctg gcaccggaag gccacctctt gtggctttct gctggtgccc
     4261 gtgctggagg gcccatttgc cctgccatcc tacctgtacg gcgatcccct gagagcccag
     4321 ctgtttatcc ccctgaacat ctcctgtctg ctgaaggagg gctctgagca cctgtttgac
     4381 agcttcgagc ctgagaccta ctgggacaga atgcacctgt ttcaggaggc catcgcccac
     4441 aggttcggct ttgtgcagga taagtactcc gccagcgcct ttaatttccc cgccgagaat
     4501 aagccacagt acatccacgt gacaggcacc gtgttcctgc agctgccata ctccaagcgg
     4561 aagttttccg gccagcagcg gaggcggaga aattccacca gctctacaaa ccagaacatg
     4621 ttctgtgagg agagagtggg ctacaattgg gcctacaaca ccatgctgac aaagacatgg
     4681 agatcctctg ccacaggcga cgagaagttc gccgatcggc tgctgaagga ctttacagac
     4741 ttctgcatca atcgggacaa tcgcctggtg accttctgga caagttgcct ggaaaaaatg
     4801 cacgcctccg ccccctgact cgag
//
