LOCUS       sequence-320474-        1752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLCN expressing vector for mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Lawrence RE, Fromm SA, Fu Y, Yokom AL, Kim DJ, Thelen AM, Young LN, 
            Lim CY, Samelson AJ, Hurley JH, Zoncu R
  TITLE     Structural mechanism of a Rag GTPase activation checkpoint by the 
            lysosomal folliculin complex.
  JOURNAL   Science. 2019 Nov 22;366(6468):971-977. doi: 
            10.1126/science.aax0364. Epub 2019 Oct 31.
  PUBMED    31672913
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aax0364"; journalName: "Science"; date: 
            "2019-11-22- 22"; volume: "366"; issue: "6468"; pages: "971-977"
COMMENT     Sequence Label: pCAG-TWIN-STREP-FLAG-FLCN
FEATURES             Location/Qualifiers
     source          1..1752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggtaccatga acgccatcgt ggccctgtgc cacttctgtg agctgcacgg cccacggacc
       61 ctgttttgca cagaggtgct gcacgcacca ctgccacagg gcgacggaaa cgaggatagc
      121 ccaggacagg gagagcaggc agaggaggag gagggcggca tccagatgaa tagccggatg
      181 agagcccact ccccagcaga gggagcctct gtggagagct cctctcctgg cccaaagaag
      241 agcgacatgt gcgagggatg tcggtccctg gccgcaggac accctggcta tatctctcac
      301 gataaggaga caagcatcaa gtacgtgtct caccagcacc ccagccaccc tcagctgttc
      361 tccatcgtgc ggcaggcctg cgtgagatcc ctgtcttgcg aggtgtgccc aggaagagag
      421 ggacctatct tctttggcga cgagcagcac ggcttcgtgt tttctcacac cttctttatc
      481 aaggatagcc tggccagggg cttccagcgg tggtattcca tcatcacaat catgatggac
      541 cggatctacc tgatcaactc ttggcccttt ctgctgggca aggtgagagg catcatcgac
      601 gagctgcagg gcaaggccct gaaggtgttc gaggccgagc agtttggctg tccacagagg
      661 gcccagagga tgaacaccgc cttcacacct tttctgcacc agaggaacgg caatgccgcc
      721 cgctctctga ccagcctgac atccgacgat aatctgtggg cctgcctgca caccagcttt
      781 gcctggctgc tgaaggcctg tggctccagg ctgacagaga agctgctgga gggcgcccct
      841 accgaggaca cactggtgca gatggagaag ctggccgacc tggaggagga gagcgagtcc
      901 tgggataata gcgaggcaga agaggaggag aaggcaccag tgctgccaga gtccaccgag
      961 ggaagggagc tgacacaggg accagccgag agctcctctc tgtctggctg cggcagctgg
     1021 cagcctagaa agctgccagt gttcaagtct ctgaggcaca tgagacaggt gctgggcgcc
     1081 cccagcttta ggatgctggc ctggcacgtg ctgatgggca accaggtcat ctggaagtcc
     1141 cgcgacgtgg atctggtgca gtctgccttc gaggtgctga ggaccatgct gccagtgggc
     1201 tgcgtgcgca tcatccccta cagctcccag tatgaggagg cctacaggtg taatttcctg
     1261 ggcctgagcc ctcacgtgca gatcccacct cacgtgctgt ctagcgagtt tgccgtgatc
     1321 gtggaggtgc acgccgccgc ccggagcacc ctgcacccag tgggctgtga ggacgatcag
     1381 tctctgagca agtatgagtt tgtggtgaca tccggctctc ctgtggcagc agacagggtg
     1441 ggcccaacca tcctgaacaa gatcgaggcc gccctgacaa accagaatct gagcgtggac
     1501 gtggtggatc agtgcctggt gtgcctgaag gaggagtgga tgaataaggt gaaggtgctg
     1561 ttcaagttta ccaaggtgga ctcccgccct aaggaggata cacagaagct gctgtccatc
     1621 ctgggcgcct ctgaggagga taacgtgaag ctgctgaagt tctggatgac cggcctgagc
     1681 aagacataca agtcccacct gatgtctacc gtgcggagcc caacagccag cgagtccaga
     1741 aattgactcg ag
//
