LOCUS       sequence-320475-       11897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full 
            length human O-GlcNAc transferase, WT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11897)
  AUTHORS   Levine ZG, Potter SC, Joiner CM, Fei GQ, Nabet B, Sonnett M, Zachara
            NE, Gray NS, Paulo JA, Walker S
  TITLE     Mammalian cell proliferation requires noncatalytic functions of 
            O-GlcNAc transferase.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2016778118. doi: 
            10.1073/pnas.2016778118.
  PUBMED    33419956
REFERENCE   2  (bases 1 to 11897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-26"; volume: "118(4). pii: 
            2016778118. doi"; pages: " 10.1073/pnas.2016778118"
COMMENT     Sequence Label: pLenti PGK mKate2-OGT WT (Puro)
FEATURES             Location/Qualifiers
     source          1..11897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(106..174,175..966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(324..343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1626..1645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1879..1896
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2013..2034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2049..2054,2055..2072,2073..2079)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2087..2103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2092..2114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2111..2127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2111..2127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2146..2166
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2148..2166
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2194..2420
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2421..2601
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2648..2773
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3266..3499
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3684..3728
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3995..4112
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4158..4668
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4587..4605
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    4723..4747
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4778..5473
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             5480..5503
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5507..5530
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    5546..5570
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8695..8719)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8750..9338
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8803..8823)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9221..9232)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9352..9851
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9387..9410)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9784..9803
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9872..10471
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9872..9891)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10368..10388
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10609..10842
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10914..11035
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10951..10970)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11005..11024
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11075..11210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11230..11249)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11231..11249)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11259..11276)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11259..11275)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11268..11290)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11417..11872
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11504..11523)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11714..11735
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1801 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1861 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     1921 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     1981 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2041 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     2101 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     2161 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     2221 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     2281 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     2341 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     2401 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     2461 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     2521 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     2581 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     2641 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     2701 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     2761 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     2821 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     2881 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     2941 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     3001 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     3061 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     3121 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     3181 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     3241 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     3301 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     3361 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     3421 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     3481 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     3541 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     3601 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     3661 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     3721 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     3781 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     3841 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     3901 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     3961 gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg
     4021 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     4081 tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgagaagc
     4141 ttgatatcga attccacggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg
     4201 cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct
     4261 cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc
     4321 cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg
     4381 tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc
     4441 cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca
     4501 gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta
     4561 gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg
     4621 tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccaggg ggatccaccg
     4681 gttcgtcgac tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa
     4741 gcaggcttta aaggaaccaa ttcagtcgac tggatccatg gtgagcgagc tgattaagga
     4801 gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac
     4861 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggcggtcga
     4921 gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcaa
     4981 aaccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct tccccgaggg
     5041 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
     5101 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc
     5161 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggcctcca ccgagaccct
     5221 gtaccccgct gacggcggcc tggaaggcag agccgacatg gccctgaagc tcgtgggcgg
     5281 gggccacctg atctgcaact tgaagaccac atacagatcc aagaaacccg ctaagaacct
     5341 caagatgccc ggcgtctact atgtggacag aagactggaa agaatcaagg aggccgacaa
     5401 agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa
     5461 actggggcac agaggcggag actacaaaga tgatgacgac aaaggcgact acaaagacga
     5521 tgacgacaag ggcatcgata gatcaacaag tttgtacaaa aaagcaggct ttgcgtcttc
     5581 cgtgggcaac gtggccgaca gcacagggtt agctgagttg gcacatcgag aatatcaggc
     5641 aggagatttt gaggcagctg agagacactg catgcagctc tggagacaag agccagacaa
     5701 tactggtgtg cttttattac tttcatctat acacttccag tgtcgaaggc tggacagatc
     5761 tgctcacttt agcactctgg caattaaaca gaaccccctt ctggcagaag cttattcgaa
     5821 tttggggaat gtgtacaagg aaagagggca gttgcaggag gcaattgagc attatcgaca
     5881 tgcattgcgt ctcaaacctg atttcatcga tggttatatt aacctggcag ccgccttggt
     5941 agcagcgggt gacatggaag gggcagtaca agcttacgtc tctgctcttc agtacaatcc
     6001 tgatttgtac tgtgttcgca gtgacctggg gaacctgctc aaagccctgg gtcgcttgga
     6061 agaagccaag gcatgttatt tgaaagcaat tgagacgcaa ccgaactttg cagtagcttg
     6121 gagtaatctt ggctgtgttt tcaatgcaca aggggaaatt tggcttgcaa ttcatcactt
     6181 tgaaaaggct gtcacccttg acccaaactt tctggatgct tatatcaatt taggaaatgt
     6241 cttgaaagag gcacgcattt ttgacagagc tgtggcagct tatcttcgtg ccctaagttt
     6301 gagtccaaat cacgcagtgg tgcacggcaa cctggcttgt gtatactatg agcaaggcct
     6361 gatagatctg gcaatagaca cctacaggcg ggctatcgaa ctacaaccac atttccctga
     6421 tgcttactgc aacctagcca atgctctcaa agagaagggc agtgttgctg aagcagaaga
     6481 ttgttataat acagctctcc gtctgtgtcc cacccatgca gactctctga ataacctagc
     6541 caatatcaaa cgagaacagg gaaacattga agaggcagtt cgcttgtatc gtaaagcatt
     6601 agaagtcttc ccagagtttg ctgctgccca ttcaaattta gcaagtgtac tgcagcagca
     6661 gggaaaactg caggaagctc tgatgcatta taaggaggct attcgaatca gtcctacctt
     6721 tgctgatgcc tactctaata tgggaaacac tctaaaggag atgcaggatg ttcagggagc
     6781 cttgcagtgt tatacgcgtg ccatccaaat taatcctgca tttgcagatg cacatagcaa
     6841 tctggcttcc attcataagg attcagggaa tattccagaa gccatagctt cttaccgcac
     6901 ggctctgaaa cttaagcctg attttcctga tgcttattgt aacttggctc attgcctgca
     6961 gattgtctgt gattggacag actatgatga gcgaatgaag aagttggtca gtattgtggc
     7021 tgaccagtta gagaagaata ggttgccttc tgtgcatcct catcatagta tgctatatcc
     7081 tctttctcat ggcttcagga aggctattgc tgagaggcac ggcaacctgt gcttagataa
     7141 gattaatgtt cttcataaac caccatatga acatccaaaa gacttgaagc tcagtgatgg
     7201 tcggctgcgt gtaggatatg tgagttccga ctttgggaat catcctactt ctcaccttat
     7261 gcagtctatt ccaggcatgc acaatcctga taaatttgag gtgttctgtt atgccctgag
     7321 cccagacgat ggcacaaact tccgagtgaa ggtgatggca gaagccaatc atttcattga
     7381 tctttctcag attccatgca atggaaaagc agctgatcgc atccatcagg atggaattca
     7441 tatccttgta aatatgaatg gctatactaa gggcgctcga aatgagcttt ttgctctcag
     7501 gccagctcct attcaggcaa tgtggctggg ataccctggg acgagtggtg cgcttttcat
     7561 ggattatatt atcactgatc aggaaacttc gccagctgaa gttgctgagc agtattccga
     7621 gaaattggct tatatgcccc acactttttt tattggtgat catgctaata tgttccctca
     7681 cctgaagaaa aaagcagtca tcgattttaa gtccaatggg cacatttatg acaatcggat
     7741 agttctgaat ggcatcgacc tcaaagcatt tcttgatagt ctaccagatg tgaaaattgt
     7801 caagatgaag tgtcctgatg gaggagacaa tgcagatagc agtaacacag ctcttaatat
     7861 gcctgttatt cctatgaata ctattgcaga agcagttatt gaaatgatta accgaggaca
     7921 gattcaaata acaattaatg gattcagtat tagcaatgga ctggcaacta ctcagatcaa
     7981 caataaggct gcaactggag aggaggttcc ccgtaccatt attgtaacca cccgttctca
     8041 gtacgggtta ccagaagatg ccatcgtata ctgtaacttt aatcagttgt ataaaattga
     8101 cccttctact ttgcagatgt gggcaaacat tctgaagcgt gttcccaata gtgtactctg
     8161 gctgttgcgt tttccagcag taggagaacc taatattcaa cagtatgcac aaaacatggg
     8221 cctgccccag aaccgtatca ttttttcacc tgttgctcct aaagaggaac acgtcaggag
     8281 aggccagctg gctgatgtct gcttggacac tccactctgt aatgggcaca ccacagggat
     8341 ggatgtcctc tgggcaggga cccccatggt gactatgcca ggagagactc ttgcttctcg
     8401 agttgcagca tcccagctca cttgcttagg ttgtcttgag cttattgcta aaaacagaca
     8461 agaatatgaa gacatagctg tgaagctggg aactgatcta gaatacctga agaaagttcg
     8521 tggcaaagtc tggaagcaaa gaatatctag ccctctgttc aacaccaaac aatacacaat
     8581 ggaactagag cggctctatc tacagatgtg ggagcattat gcagctggca acaaacctga
     8641 ccacatgatt aagcctgttg aagtcactga gtcagcataa ctcgagatat ctagacccag
     8701 ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgaca atcaacctct
     8761 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     8821 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     8881 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     8941 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     9001 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     9061 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     9121 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     9181 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     9241 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     9301 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattctac cgggtagggg
     9361 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     9421 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     9481 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     9541 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     9601 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     9661 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     9721 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     9781 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     9841 cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct
     9901 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     9961 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
    10021 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
    10081 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
    10141 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
    10201 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
    10261 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
    10321 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
    10381 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
    10441 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
    10501 cgaaaggagc gcacgacccc atgcatctcg agggcccggt acctttaaga ccaatgactt
    10561 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctag
    10621 ctcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
    10681 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
    10741 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
    10801 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
    10861 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
    10921 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    10981 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    11041 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
    11101 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    11161 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
    11221 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
    11281 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    11341 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    11401 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    11461 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    11521 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    11581 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    11641 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    11701 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    11761 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    11821 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
    11881 cttttcgggg aaatgtg
//
