LOCUS       sequence-320486-         545 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of gRNA targeting TCRbeta constant locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Lee MN, Meyerson M
  TITLE     Antigen identification for HLA class I- and HLA class II-restricted 
            T cell receptors using cytokine-capturing antigen-presenting cells.
  JOURNAL   Sci Immunol. 2021 Jan 22;6(55). pii: 6/55/eabf4001. doi: 
            10.1126/sciimmunol.abf4001.
  PUBMED    33483338
REFERENCE   2  (bases 1 to 545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2021-01-22"; volume: "6(55). pii: 6/55/eabf4001. 
            doi"; pages: " 10.1126/sciimmunol.abf4001"
COMMENT     Sequence Label: CRISPR_TRBC
FEATURES             Location/Qualifiers
     source          1..545
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     110..129
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        209..284
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    332..449
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
       61 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
      121 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
      181 aaacaccgcg tagaactgga cttgacaggt tttagagcta gaaatagcaa gttaaaataa
      241 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt gaattctaga
      301 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
      361 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
      421 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
      481 tttggcgccg gctcgagtgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
      541 gtccc
//
