LOCUS       sequence-320525-        4925 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses untagged human RDH11. Confers G418 resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Demange P, Joly E, Marcoux J, Zanon PRA, Listunov D, Rulliere P, 
            Barthes C, Noirot C, Izquierdo JB, Rozie A, Pradines K, Hee R, de 
            Brito MV, Marcellin M, Serre RF, Bouchez O, Burlet-Schiltz O, 
            Oliveira MCF, Ballereau S, Bernardes-Genisson V, Maraval V, Calsou 
            P, Hacker SM, Genisson Y, Chauvin R, Britton S
  TITLE     SDR enzymes oxidize specific lipidic alkynylcarbinols into cytotoxic
            protein-reactive species.
  JOURNAL   Elife. 2022 May 10;11. pii: 73913. doi: 10.7554/eLife.73913.
  PUBMED    35535493
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-05-10"; volume: "11. pii: 73913. doi"; pages: " 
            10.7554/eLife.73913"
COMMENT     Sequence Label: pE-N1-RDH11
FEATURES             Location/Qualifiers
     source          1..4925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      66..75
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1151..1272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1188..1207)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1242..1261
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1279..1734)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1416..1437)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1628..1647
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1761..1865
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1867..2224
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1867..2058
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1890..1910)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2075..2210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2259..3053
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2259..3053
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2313..2332)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2923..2942
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3241..3260)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3285..3332
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3661..4249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4150..4169
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4424..4727
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(4728..4925,1..6)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4882..4902
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat ctcgagctca
       61 agcttgccac catggttgag ctcatgttcc cgctgttgct cctccttctg cccttccttc
      121 tgtatatggc tgcgccccaa atcaggaaaa tgctgtccag tggggtgtgt acatcaactg
      181 ttcagcttcc tgggaaagta gttgtggtca caggagctaa tacaggtatc gggaaggaga
      241 cagccaaaga gctggctcag agaggagctc gagtatattt agcttgccgg gatgtggaaa
      301 agggggaatt ggtggccaaa gagatccaga ccacgacagg gaaccagcag gtgttggtgc
      361 ggaaactgga cctgtctgat actaagtcta ttcgagcttt tgctaagggc ttcttagctg
      421 aggaaaagca cctccacgta ttgatcaaca atgcaggagt gatgatgtgt ccgtactcga
      481 agacagcaga tggctttgag atgcacatag gagtcaacca cttgggtcac ttcctcctaa
      541 cccatctgct gctagagaaa ctaaaggaat cagccccatc aaggatagta aatgtgtctt
      601 ccctcgcaca tcacctggga aggatccact tccataacct gcagggcgag aaattctaca
      661 atgcaggcct ggcctactgt cacagcaagc tagccaacat cctcttcacc caggaactgg
      721 cccggagact aaaaggctct ggcgttacga cgtattctgt acaccctggc acagtccaat
      781 ctgaactggt tcggcactca tctttcatga gatggatgtg gtggcttttc tcctttttca
      841 tcaagactcc tcagcaggga gcccagacca gcctgcactg tgccttaaca gaaggtcttg
      901 agattctaag tgggaatcat ttcagtgact gtcatgtggc atgggtctct gcccaagctc
      961 gtaatgagac tatagcaagg cggctgtggg acgtcagttg tgacctgctg ggcctcccaa
     1021 tagactaaag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
     1081 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     1141 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     1201 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     1261 caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     1321 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     1381 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     1441 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     1501 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     1561 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     1621 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     1681 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     1741 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     1801 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     1861 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     1921 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     1981 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     2041 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     2101 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     2161 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     2221 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     2281 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     2341 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     2401 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     2461 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     2521 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     2581 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     2641 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     2701 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     2761 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     2821 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     2881 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     2941 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     3001 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     3061 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     3121 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     3181 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     3241 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     3301 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     3361 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     3421 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     3481 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
     3541 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3601 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3661 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3721 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3781 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3841 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3901 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3961 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4021 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4081 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4141 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4201 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4261 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4321 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
     4381 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     4441 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     4501 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     4561 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     4621 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     4681 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     4741 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     4801 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     4861 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     4921 taagc
//
