LOCUS       sequence-320556-       10356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This insert, when expressed in the same cell with other split Cre 
            component, forms Cre recombinase through protein trans-splicing 
            using split intein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10356)
  AUTHORS   Luan H, Kuzin A, Odenwald WF, White BH
  TITLE     Cre-assisted fine-mapping of neural circuits using orthogonal split 
            inteins.
  JOURNAL   Elife. 2020 Apr 14;9. pii: 53041. doi: 10.7554/eLife.53041.
  PUBMED    32286225
REFERENCE   2  (bases 1 to 10356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-04-14"; volume: "9. pii: 53041. doi"; pages: " 
            10.7554/eLife.53041"
COMMENT     Sequence Label: HJP-178-IVS-Syn21-CreAB-NrdJ-1N-WPREw
FEATURES             Location/Qualifiers
     source          1..10356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..125
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(150..155,156..173,174..180)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             234..893
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(300..319)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1235..1540
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1419..1438
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1581..1705)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          1744..1810
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     misc_feature    2921..3509
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2974..2994)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3392..3403)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3770..3786)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3770..3786)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(4797..5411,5482..5613,5817..6132,6194..6848,
                     6923..7196,7598..7669))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     8121..8139
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    8364..8433
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(8520..9108)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8600..8619)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9279..10070,10071..10139))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9902..9921
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10140..10244)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10311..10329
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
       61 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      121 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
      181 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
      241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      361 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      421 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      481 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      541 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      601 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      661 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
      721 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
      781 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
      841 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
      901 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
      961 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     1021 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1081 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1141 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     1201 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     1261 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     1321 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     1381 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     1441 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     1501 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     1561 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     1621 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     1681 gttcagcttt cttgtacaaa gtggtgataa acggccggcc agatctggta ccgctagaaa
     1741 aaggtaggtt caaccactga tgcctaggca caccgaaacg actaacccta attcttatcc
     1801 tttacttcag ggctagcaac ttaaaaaaaa aaatcaaaat gagtaacttg ctcacggtac
     1861 accaaaattt gccggccctg cctgtggacg ccacgtccga cgaggtccga aagaatctga
     1921 tggacatgtt ccgtgacagg caggccttta gcgagcacac ctggaagatg ctgctctcgg
     1981 tgtgcaggtc ctgggccgct tggtgcaagc tgaacaaccg aaagtggttt cccgcggagc
     2041 ccgaggacgt ccgcgattat ctgctgtacc tgcaggctcg cggactggcc gtcaaaacaa
     2101 tacagcaaca tttgggtcag ctgaacatgc tccacaggcg cagcggactg ccccggccat
     2161 ccgatagcaa tgcagtgagc ctggtaatgc gtcgaatacg caaggaaaac gtggacgccg
     2221 gcgagagggc caaacaggcg ttggctttcg aacgcaccga tttcgatcag gtgcggtcgt
     2281 tgatggagaa cagcgatcga tgccaggaca tccggaactt ggcgtttttg ggcattgcct
     2341 ataacaccct ccttcgcatc gccgagatag cgcgtatccg agttaaagac atttcccgta
     2401 cagacggagg ccgcatgctt attcacatcg gacgcacgaa aaccctggtc agcactgctg
     2461 gcgtggagaa agcactttcc ctgggtgtga cgaaattggt cgagcgatgg atcagtgtga
     2521 gtggcgtcgc cgatgacccc aacaattatc tgttctgcag ggtgcgcaaa aatggagtgg
     2581 cagccccctg cctggtgggc agcagcgaga tcatcacccg caactacggc aagaccacca
     2641 tcaaggaggt ggtggagatc ttcgataacg ataagaacat ccaggtgctg gccttcaaca
     2701 cccacaccga taacatcgag tgggccccca tcaaggccgc ccagctgacc cgccccaacg
     2761 ccgagctggt ggagctggag atcgataccc tgcacggcgt gaagaccatc cgctgcaccc
     2821 ccgatcaccc cgtgtacacc aagaaccgcg gctacgtgcg cgccgatgag ctgaccgatg
     2881 atgatgagct ggtggtggcc atctaaaccg gtgtttaaac aatcaacctc tggattacaa
     2941 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     3001 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     3061 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     3121 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     3181 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3241 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3301 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     3361 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3421 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3481 tcggatctcc ctttgggccg cctccccgcc tgaagcttat cgataccgtc gactaaagcc
     3541 aaatagaaaa ttattcagtt cctggcttaa gtttttaaaa gtgatattat ttatttggtt
     3601 gtaaccaacc aaaagaatgt aaataactaa tacataatta tgttagtttt aagttagcaa
     3661 caaattgatt ttagctatat tagctacttg gttaataaat agaatatatt tatttaaaga
     3721 taattgcgtt tttattgtca gggagtgagt ttgcttaaaa actcgtttag atccactagt
     3781 tctagagcgg ccgctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc
     3841 taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt
     3901 gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt
     3961 tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga
     4021 ggcgcgccct agttccagtg aaatccaagc attttctaaa ttaaatgtat tcttattatt
     4081 atagttgtta tttttgatat atataaacaa cactattatg cccaccattt ttttgagatg
     4141 catctacaca aggaacaaac actggatgtc actttcagtt caaattgtaa cgctaatcac
     4201 tccgaacagg tcacaaaaaa ttaccttaaa aagtcataat attaaattag aataaatata
     4261 gctgtgaggg aaatatatac aaatatattg gagcaaataa attgtacata caaatattta
     4321 ttactaattt ctattgagac gaaatgaacc actcggaacc atttgagcga accgaatcgc
     4381 gcggaactaa cgacagtcgc tccaaggtcg tcgaacaaaa ggtgaatgtg ttgcggagag
     4441 cgggtgggag acagcgaaag agcaactacg aaacgtggtg tggtggaggt gaattatgaa
     4501 gagggcgcgc gatttgaaaa gtatgtatat aaaaaatata tcccggtgtt ttatgtagcg
     4561 ataaacgagt ttttgatgta aggtatgcag gtgtgtaagt cttttggtta gaagacaaat
     4621 ccaaagtcta cttgtgggga tgttcgaagg ggaaatactt gtattctata ggtcatatct
     4681 tgtttttatt ggcacaaata taattacatt agctttttga gggggcaata aacagtaaac
     4741 acgatggtaa taatggtaaa aaaaaaaaac aagcagttat ttcggatata tgtcggctac
     4801 tccttgcgtc gggcccgaag tcttagagcc agatatgcga gcacccggaa gctcacgatg
     4861 agaatggcca gacccacgta gtccagcggc agatcggcgg cggagaagtt aagcgtctcc
     4921 aggatgacct tgcccgaact ggggcacgtg gtgttcgacg atgtgcagct aatttcgccc
     4981 ggctccacgt ccgcccattg gttaatcagc agaccctcgt tggcgtaacg gaaccatgag
     5041 aggtacgaca accatttgag gtatactggc accgagcccg agttcaagaa gaagccgcca
     5101 aagagcagga atggtatgat aaccggcgga cccacagaca gcgccatcga ggtcgaggag
     5161 ctggcgcagg atattagata tccgaaggac gttgacacat tggccaccag agtgaccagc
     5221 gccaggcagt tgaagaagtg cagcactccg gcccgcagtc cgatcatcgg ataggcaatc
     5281 gccgtgaaga ccagtggcac tgtgagaaaa agcggcaatt cggcaatcgt tttgcccaga
     5341 aagtatgtgt cacagcgata aagtcgactt cgggcctccc tcataaaaac tggcagctct
     5401 gaggtgaaca cctaaatcga atcgattcat tagaaagtta gtaaattatt gaaatgcaaa
     5461 tgtattctaa acatgactta catttatcgt ggcaaagacg ttttgaaagg tcatgttggt
     5521 caggaagagg aagatggctc cgttgatatt catcacaccc acttgcgtga gttgttggcc
     5581 caaaaagatg aggccaatca agatggcaac catctgcaaa ttaaaatgtt actcgcatct
     5641 cattaatatt cgcgagttaa atgaaattta tttatcttct gcaaaactat aaactataca
     5701 tctcattgaa aaaaactaag aagggtgtgg aatcaggcaa ttctatctaa aatctagcga
     5761 atttgtttcc aagaattgta agcgttatat catttgtttc cactggaacc actcaccgtt
     5821 gtctgaataa gtcgcacttt tacgaggagt ggttccttga gcaccgacag ccaggatcgc
     5881 cacaggaccg cccggaactg catgaaccag gtggccttgt aggtgtaccc attctccggc
     5941 tgctccagtg gcttctccag atttttggtg gccaacaact gctccatatc ccgggctact
     6001 ttgctaatgg caaaattgtc gcatatcttg gcgatccgat cacgggactc gatctcccgt
     6061 ccgggcacaa cggccaacac ctgtacgtaa aagtccgccg gattgtagtt ggtaggacac
     6121 tgggcaccca cgctggatag gagttgagat gttatgtaat actagatacc cttaataaac
     6181 acatcgaact cactaggaaa agaagtcgac ggcttcgctg ggagtgccca agaaagctac
     6241 cctgccctcg gccatcagaa ggatcttgtc aaagagctca aacagctcgg aagacggctg
     6301 atgaatggtc aggatgacgg tcttgccctt ctgcgacagc ttcttcagca cctggacgac
     6361 gctgtgggcg gtaaaggagt ccagtccgga ggtgggctca tcgcagatca gaagcggcgg
     6421 atcggttaga gcctcggagg cgaatgccag acgcttcctt tctccgccgg acagaccttt
     6481 caccctgccg ggcacaccga tgatcgtgtg ctgacatttg ctgagcgaaa gctcctggat
     6541 cacctgatcc acgcgggcca ctcgctgccg ataggtcaga tgtcgtggca tccgcaccat
     6601 ggcttggaaa atcaggtgtt ccctggccgt tagggagccg ataaagaggt catcctgctg
     6661 gacataggcg cacctggcct gcatctcctt ggcgtccaca ggttggccat tgagcagtcg
     6721 catcccggat ggcgatactt ggatgccctg cggcgatcga aaggcaaggg cattcagcag
     6781 ggtcgtcttt ccggcaccgg aactgcccat cacggccaaa agttcgcccg gataggccac
     6841 gccgcaaact gagtttcaaa ttggtaattg gaccctttat taagatttca cacagatcag
     6901 ccgactgcga atagaaactc accgttcttg agcaaatgtt tcctgggcgc cggtatgtgt
     6961 cgctcgttgc agaatagtcc gcgtgtccgg ttgaccagct gccgccatcc ggagcccggc
     7021 tgattgaccg ccccaaagat gtccatattg tgccaggcat aggtgaggtt ctcggctagt
     7081 tggccgctcc ctgaaccgga gtcctccggc ggactgggtg gccggagcgt gccgtagttt
     7141 ttggcctgcc cgaagccctg gttaatgcag ctctgcgaag ccgctccgct gtcaccctgc
     7201 aatgataggg gatctcaaat atcaactaca agcgttatgc tcatctaacc ccgaacaaaa
     7261 agtaccccga agtatcctac gaagtaggtt tatactttta tttatttttt gtgcatctag
     7321 gatcagctta aaatatctgg ttgttatatt ttttgtaaaa aagaatatag tcgaaaatga
     7381 atgcctttag atgtcttgat catgatatga tctcaaaaat tgtcttatat agcgagaaca
     7441 gctaccagaa taatctgttt cgtgtcacta tttgtttgtg caattgcggt ttgggatttt
     7501 tgtgggtcgc agttctcacg ccgcagacaa tttgatgttg caatcgcagt tcctatagat
     7561 caagtgaact taagatgtat gcacatgtac tactcacatt gttcagatgc tcggcagatg
     7621 ggtgtttgct gcctccgcga attaatagct cctgatcctc ttggcccatt gccgggattt
     7681 ttcacacttt cccctgctta cccacccaaa accaatcacc accccaatca ctcaaaaaac
     7741 aaacaaaaat aagaagcgag aggagttttg gcacagcact ttgtgtttaa ttgatggcgt
     7801 aaaccgcttg gagcttcgtc acgaaaccgc tgacaaaatg caactgaagg cggacattga
     7861 cgctacgtaa cgctacaaac ggtggcgaaa gagatagcgg acgcagcggc gaaagagacg
     7921 gcgatatttc tgtggacaga gaaggaggca aacagcgctg actttgagtg gaatgtcatt
     7981 ttgagtgaga ggtaatcgaa agaacctggt acatcaaata cccttggatc gaagtaaatt
     8041 taaaactgat cagataagtt caatgatatc cagtgcagta aaaaaaaaaa atgttttttt
     8101 tatctacttt ccgcaaaaat gggttttatt aacttacata catactaggc gcgcccatat
     8161 gttcggcttg tcgacatgcc cgccgtgacc gtcgagaacc cgctgacgct gccccgcgta
     8221 tccgcacccg ccgacgccgt cgcacgtccc gtgctcaccg tgaccaccgc gcccagcggt
     8281 ttcgagggcg agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta ccgccacctc
     8341 gacccgttca tcatgatgga ccagatgggt gaggtggagt acgcgcccgg ggagcccaag
     8401 ggcacgccct ggcacccgca ccgcggcttc gagaccgtga cctacatcgt cgacggtaac
     8461 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8521 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8581 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8641 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8701 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8761 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8821 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8881 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8941 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9001 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9061 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9121 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9181 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     9241 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     9301 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9361 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9421 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9481 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9541 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9601 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9661 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9721 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9781 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9841 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9901 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9961 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    10021 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    10081 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    10141 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    10201 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    10261 gtgccacctg agtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
    10321 tcacgaggcc ctttcgtctt caagaattcg tttatc
//
