LOCUS       sequence-320560-        9136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fast cycling ARF mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9136)
  AUTHORS   Nacke M, Sandilands E, Nikolatou K, Roman-Fernandez A, Mason S, 
            Patel R, Lilla S, Yelland T, Galbraith LCA, Freckmann EC, McGarry L,
            Morton JP, Shanks E, Leung HY, Markert E, Ismail S, Zanivan S, Blyth
            K, Bryant DM
  TITLE     An ARF GTPase module promoting invasion and metastasis through 
            regulating phosphoinositide metabolism.
  JOURNAL   Nat Commun. 2021 Mar 12;12(1):1623. doi: 10.1038/s41467-021-21847-4.
  PUBMED    33712589
REFERENCE   2  (bases 1 to 9136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-12"; volume: "12(1):1623. doi"; pages: " 
            10.1038/s41467-021-21847-4"
COMMENT     Sequence Label: pLEX301-ARF6-T157N-TagRFP
FEATURES             Location/Qualifiers
     source          1..9136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             74..673
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(74..93)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     570..590
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    715..832
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        949..1252
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1253..1456
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1407..1427
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2015..2024
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2021..2752
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     misc_feature    2831..3419
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2884..2904)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3302..3313)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3491..3724
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3802..3923
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3839..3858)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3893..3912
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3963..4098
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4118..4137)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4119..4137)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4147..4164)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4147..4163)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4156..4178)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4305..4760
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4392..4411)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4602..4623
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4786..4890
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4891..4959,4960..5751)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5109..5128)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5922..6510
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6411..6430
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6664..6681
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6798..6819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6834..6839,6840..6857,6858..6864)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6872..6888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6877..6899
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6896..6912
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6896..6912
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6931..6951
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6933..6951
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6979..7205
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7206..7386
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7433..7558
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8051..8284
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8469..8513
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        join(8688..9136,1..52)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9107..9125
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agggggtacc
       61 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
      121 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
      181 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
      241 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
      301 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
      361 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
      421 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
      481 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
      541 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
      601 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
      661 gcccggtgcc tgagaattct agatcttgag acaaatggca gtattcatcc acaattttaa
      721 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
      781 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
      841 ttacagggac agcagagatc cactttggcg ccggctcgag ggggcccggg gttattaata
      901 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      961 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     1021 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     1081 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     1141 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     1201 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     1261 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     1321 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     1381 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     1441 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagatgggga aggtgctatc
     1501 caaaatcttc gggaacaagg aaatgcggat cctcatgttg ggcctggacg cggccggcaa
     1561 gacaacaatc ctgtacaagt tgaagctggg ccagtcggtg accaccattc ccactgtggg
     1621 tttcaacgtg gagacggtga cttacaaaaa tgtcaagttc aacgtatggg atgtgggcgg
     1681 ccaggacaag atccggccgc tctggcggca ttactacact gggacccaag gtctcatctt
     1741 cgtagtggac tgcgccgacc gcgaccgcat cgatgaggct cgccaggagc tgcaccgcat
     1801 tatcaatgac cgggagatga gggacgccat aatcctcatc ttcgccaaca agcaggacct
     1861 gcccgatgcc atgaaacccc acgagatcca ggagaaactg ggcctgaccc ggattcggga
     1921 caggaactgg tatgtgcagc cctcctgtgc caactcaggg gacggactct atgagggcct
     1981 gacctggctg accagcaact acaaatctcc ggtcgccacc atggtgtcta agggcgaaga
     2041 gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtga acaaccacca
     2101 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
     2161 caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat
     2221 gtacggcagc agaaccttca tcaaccacac ccagggcatc cccgatttct ttaagcagtc
     2281 cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac
     2341 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
     2401 ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccaa
     2461 caccgagatg ctgtaccccg ctgacggcgg cctggaaggc agaaccgaca tggccctgaa
     2521 gctcgtgggc gggggccacc tgatctgcaa cttcaagacc acatacagat ccaagaaacc
     2581 cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac cacagactgg aaagaatcaa
     2641 ggaggccgac aaagagacct acgtcgagca gcacgaggtg gctgtggcca gatactgcga
     2701 cctccctagc aaactggggc acaaacttaa tggcatggac gagctgtaca agtccggact
     2761 cagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc cggtggcgcg
     2821 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     2881 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     2941 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     3001 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     3061 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     3121 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     3181 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     3241 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     3301 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     3361 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     3421 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     3481 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     3541 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     3601 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     3661 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     3721 agcagtacgt atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     3781 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     3841 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3901 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     3961 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4021 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     4081 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     4141 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     4201 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     4261 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     4321 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4381 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4441 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4501 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4561 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4621 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4681 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4741 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4801 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4861 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4921 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4981 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5041 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5101 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5161 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5221 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5281 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5341 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5401 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5461 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5521 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5581 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5641 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5701 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5761 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5821 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5881 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5941 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6001 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6061 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6121 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6181 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6241 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6301 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6361 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6421 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6481 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6541 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     6601 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     6661 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     6721 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     6781 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     6841 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     6901 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     6961 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     7021 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     7081 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     7141 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     7201 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7261 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7321 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7381 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     7441 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     7501 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     7561 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     7621 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     7681 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     7741 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     7801 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     7861 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     7921 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     7981 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     8041 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     8101 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     8161 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     8221 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     8281 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     8341 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     8401 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     8461 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     8521 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     8581 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     8641 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccttg cgccttttcc
     8701 aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca
     8761 gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct
     8821 tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag
     8881 gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta
     8941 ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg
     9001 ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg
     9061 aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct
     9121 gcaagcctcc ggagcg
//
