LOCUS       sequence-320568-        8937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR repair template: add insertion site specific homology arms by
            PCR before insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8937)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 8937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1821
FEATURES             Location/Qualifiers
     source          1..8937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     103..120
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(279..306)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(398..484)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     533..555
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     547..564
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     548..564
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1456..1489
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             1499..1528
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3345..3668)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5586..5610
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5613..6638
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5613..6638
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6639..6663)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    7016..7049
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             7071..7160
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     primer_bind     complement(7189..7208)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7190..7208)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7213..7229)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7213..7229)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7331..7350)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7342..8151
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7409..7428)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8298..8886
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8787..8806
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
       61 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
      121 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
      181 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
      241 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
      301 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
      361 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
      421 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
      481 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
      541 cgacgttgta aaacgacggc cagtcttaag ctcgggcccc aaataatgct cttcgatggt
      601 ctccaaagga gaagctgtga tcaaggagtt catgcgcttc aaggttcaca tggaaggaag
      661 catgaatggt cacgagttcg aaatcgaagg agaaggggag ggccgcccgt acgagggaac
      721 tcagaccgcc aagctgaagg tcaccaaggg aggtaagttt gtgataatcc aatttcaatt
      781 cgcaatggtc atcgtttttt caggaccact tccattctca tgggatattc tctccccaca
      841 attcatgtac ggctcccgtg ctttcatcaa acacccagcc gacattccag attactacaa
      901 gcaatctttc ccagaaggct tcaagtggga gcgtgtgatg aacttcgagg atggtggagc
      961 agttacagta actcaggaca cttctctcga ggtaagtttt tactccgctt ttaacaatgg
     1021 ttgtttgaca tcattttttc aggatggcac tttgatctac aaggtcaagc tccgtggtac
     1081 caatttccca ccagatggac cagttatgca gaagaagacg atgggatggg aggcttccac
     1141 cgaacgattg tacccagaag atggagttct caagggagac atcaaaatgg cgttacgcct
     1201 caaggacgga ggtaagtttg atgaaacggt ttcgtcttat atacactaat ggtacttttc
     1261 aggacgttac ctggcggatt tcaagaccac atacaaggca aagaagccag ttcaaatgcc
     1321 aggagcatat aacgttgacc gcaagcttga tattacttcc cataatgaag actacacagt
     1381 tgtagaacaa tacgaacgat ccgagggacg tcattcgacc ggaggaatgg acgagctgta
     1441 caaggtaagt ttaaaataac ttcgtataat gtatactata cgaagttatt ttcagggaga
     1501 gcaaaagctt atctccgagg aggacctcta aggatgatcg acgccaacgt cgttgaattt
     1561 tcaaatttta aatactgaat atttgttttt tttcctatta tttatttatt ctctttgtgt
     1621 tttttttctt gctttctaaa aaattaattc aatccaaatc taaacatttt tttttctctt
     1681 tccgtctccc aattcgtatt ccgctcctct catctgaaca caatgtgcaa gtttatttat
     1741 cttctcgctt tcatttcatt aggacgtggg gggaattggt ggaaggggga aacacacaaa
     1801 aggatgatgg aaatgaaata aggacacaca atatgcaaca acattcaatt cagaaatatg
     1861 gaggaaggtt taaaagaaaa cataaaaata tatagaggag gaaggaaact agctgccctc
     1921 gattgtctat cttccattct caccaagttt caacaacttt attcagtcat tcttctctgg
     1981 ttcatttccc aaccaatctt cccgaataaa gcccatctgt cctttcggcc acattcctca
     2041 cctacatccg tactccttat ctcccgttct tatccagtct tttatcgtct ctgcgtctcg
     2101 cacactgtct cgtcggtaat gtaaaaaaaa aacaaaaaaa acttgaaaac ttgcggaaag
     2161 atttcctaat aaaaggagac gaatccggta ggaaatcgac aagtctctgc ttctcgaaga
     2221 ctaggtgagt tacatttctt tggccgaatt attttgaaat cctaaatttg ataatttcag
     2281 agatgtctgt aaaacttgcg tgttatgtga cggcttcggt cactgtcgcc actcttatgg
     2341 tatgcttcat gaccatgtca accatctact cggaggttga tggattcaga gagaagcttg
     2401 ataccgagat gaatgtgttc agagtgagtt aaattatatt ttgaatttta aataattttt
     2461 aattttctag caatctacca atggattgtg gaaggacata gttgtcatcg gaagatctag
     2521 caagcgtgtc cgttgtcaat atgaagagac caacgctacc ccaactccac atgctgatgg
     2581 atccccatct gctccaccag gtcaaccacc agcagttcca ccagtcttca accagccaaa
     2641 gactccaaat ggagccaatg gaaatggacc aacctgcaac tgcaatgctg ataacaagtg
     2701 cccagctgga ccatccggac caaagggagt tccaggagtt ccaggactcg acggagttcc
     2761 aggacttgac ggtgttccag gagttggagc tgatgatatc gctccacaac gcgagtctgt
     2821 cggatgcttc acttgcccac aaggaccagt tggaccacca ggagctcttg gaagaccagg
     2881 accacgtgga cttccaggac caagaggaca aaatggaaac ccaggaagag atggacaacc
     2941 aggacatcca ggagagcaag gatcatccgg tcaaatcgga aagatcggag agccaggacc
     3001 accaggagag aagggacgcg acgccgagca tccaatcgga agaccaggac caaagggacc
     3061 aagaggagat caaggaccaa caggaccagc tggacagaac ggtcttcacg gaccaccagg
     3121 agagccagga accgttggac cagaaggacc atctggaaag caaggacgtc aaggaccaga
     3181 cggaacccag ggagagactg gaccagacgg aagaccagga aaggatgccg agtactgcca
     3241 gtgcccagac aagtctccac catcagaggc tgtcaacgcc aaccgtggat acagaaatat
     3301 ctaaattgtt ggtgttttct aataaaatat ttgagattca gtgagacttt tttcttggcg
     3361 gcacaataaa gtttcctctt tttgttgggt cactcaacga acacaataca aatatttgag
     3421 cgctcgcatt tttctcaacc tttcgggttt aaagcacttc aaaatggaat tgaagagaga
     3481 ttgtgaaagt acatcagaca gaatcacatt ttgatagcat tcacttcact cagatgcaaa
     3541 cttttcagtg actataaaaa gtcataaaat aataacatta aaaaagcaaa taaattagcg
     3601 agagaatttt ttgacaaaaa gaaagaagag tgatagagaa gaagggaatg cttgaaagga
     3661 ttttgcattt atcttaatca ccgtcttcga gaagacgaac catggcgcca gtttcagaat
     3721 ccaagtttct aatatagttc atcacaatgt tgacgttggt ccaacctccg gcttgcataa
     3781 tctctgggat ggaaactcca gcgcgagcca tgtcacgggc agctccgaca cgggcggaat
     3841 gtccagacca agccaagtaa cgttgtccgg aatcatcctt ggctccgtag atgagacggt
     3901 gggtagcttc gaagattcct gaaaatttat tttcagctta ttcttagcta atatgttgtt
     3961 taaacttacc ttcaagagct cgagtcgaga gttgtgatgt tgcactcggg gcagcaactc
     4021 cgttcttgcg aacacgacag aacaggtagt tgtttggatc atcggcgact ccggaaaccg
     4081 aaatccagcg ctcaacgagc ttggtaactc caagggagag cgctttttcg actccggcag
     4141 tcgagaccag cgtttttgta cgtccgatgt ggatgagcat tcttcctccg tctgtgcgac
     4201 taatatcctt gacgcgaatg cgggcaattt cggcgatacg gagaagtgtg ttgtaagcaa
     4261 ttccgaggaa ggcgaggttt cggatgtcct ggcaacgatc ggaattttcc ataagagaac
     4321 ggacctgatc gaagtcggtt ctgaaaattt cgtcaagtct taggattgaa tacattaata
     4381 tttaaactta cctttcgaag gcgagggctt gcttggctct ctctccagca tcaacatttt
     4441 ccttgcggat acgtctcatt acgagagata cggcattaga atctgatggg cgtggaagtc
     4501 cagatcggcg atggagcata ttgagctgtc cgagatgttg ttggatcgtc ttcaccgcca
     4561 atccacgagc ttggaggtag agcaagtagt cacgaacgtc ttctggctcg gctgggaacc
     4621 acttgcggtt gttaagcttg caccaggcag cccaagaacg gcacactgag agaagcatct
     4681 tccaggtgtg ctcggagaag gcttgtctgt cacggaacat atccatgagg ttcttgcgaa
     4741 cttcatcact tgtcgcgtca actggaagtg ctggaagatt ttgatgaaca gtgagaagat
     4801 ttgacatatt ttcgaagttt tttagatgca ctagaacaaa gcgtgttggc ttcctctgag
     4861 cccgctttcc ttatataccc gcattctgca gccttacaga atgttctaga aggtcctaga
     4921 tgcattcgtt tgaaaatact cccggtgggt gcaaagagac gcagacggaa aatgtatctg
     4981 ggtctcttta ttgtgtacac tacttttcca tgtaccgaat gtgagtcgcc ctccttttgc
     5041 aacaagcagc tcgaatgttc tagaaaaagg tggaaaatag tataaatacc gttgaaaata
     5101 aataccgaac aacatttgct ctaattgtga aattagaaat cttcaaacta taatcatgtc
     5161 actttaccac tatttccgtc cagctcaacg ttccgttttt ggtgatcatt tttgctttcg
     5221 tcgtaaatct acacacgcgt ctcttccgtg cgagagtcca agccagcagc caaattcgtt
     5281 gactgagtat tcaacgttta tacgttgtcg gcaacgagaa ataggaaaat gcatcgggaa
     5341 atgttctttt ttcgattttt tccaaggttt tgacaaattt taccacgaat tttgctatgt
     5401 tttcaattaa aaaatatgtt attcaactgt ttctatgagg aaaataaggc tttgcatgta
     5461 attttcttat tcagcataat ttttaattaa tttgaatttt ctgtcctaac gtttattttg
     5521 ttttcttggt tatgactgat ctgaaattaa tttttgaatt ttaaggtaat atgtcaggcg
     5581 gtgccgcaag tttgtacaaa aaagcaggct ccatgaaaaa gcctgaactc accgcgacgt
     5641 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     5701 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     5761 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     5821 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
     5881 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     5941 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     6001 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     6061 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     6121 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     6181 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     6241 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     6301 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     6361 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     6421 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     6481 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     6541 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     6601 accgacgccc cagcactcgt ccgagggcaa aggaatagac ccagctttct tgtacaaagt
     6661 gggtccaatt actcttcaac atccctacat gctctttctc cctgtgctcc caccccctat
     6721 ttttgttatt atcaaaaaac ttctcttaat ttctttgttt tttagcttct tttaagtcac
     6781 ctctaacaat gaaattgtgt agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa
     6841 aaaattgtgc tccctccccc cattaataat aattctatcc caaaatctac acaatgttct
     6901 gtgtacactt cttatgtttt ttacttctga taaatttttt tgaaacatca tagaaaaaac
     6961 cgcacacaaa ataccttatc atatgttacg tttcagttta tgaccgcaat ttttaataac
     7021 ttcgtataat gtatactata cgaagttatt ttcagggtgg agccggatct gaacaaaaac
     7081 ttatttctga agaggatctt gagcaaaagc tcatctccga ggaggacctc gagcagaagt
     7141 tgatcagcga ggaagacttg acgcgaagag cgccatccag ctgatatccc ctatagtgag
     7201 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     7261 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     7321 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     7381 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     7441 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     7501 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     7561 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     7621 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     7681 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     7741 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     7801 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     7861 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     7921 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     7981 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     8041 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     8101 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     8161 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     8221 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     8281 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8341 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8401 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     8461 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8521 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8581 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8641 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8701 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8761 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     8821 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8881 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gcctttt
//
