LOCUS       sequence-320573-        4182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the DRT type 4 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG012 (DRT type 4)
FEATURES             Location/Qualifiers
     source          1..4182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..546)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      2628..2675
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2895..3554)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3469..3488
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3555..3657)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4049..4066)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg
      121 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc
      181 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
      241 acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      301 acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag
      361 ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc
      421 gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa
      481 ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga
      541 tctcaagaag atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc
      601 tcttcaaatg tagcacctga agtcagcccc atacgatata agttgtaatt ctcatgttag
      661 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc
      721 gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc aagcttagca
      781 tcggagcaaa gtaactcaat accgaacaat aaatatgagc ccttcgtgaa accgggtaag
      841 gtcaaactca taaaccaaca aaaggggaaa agtgggatat gtgaggcgtg tatgattttt
      901 atttattggg cttcgttaaa aatggtgatt taatagccct ttaaatttat cactttttaa
      961 ctaactccga gggtttatgg tcatttttga cgagaagcga cacctgtacg aggcactgct
     1021 gcggcataac tacttcccta atcagaaagg ctctatttcc gaaatccccc cttgtttcag
     1081 ctcccggacc tttacaccag agatcgccga gctgatctct agcgatacct ccggccggag
     1141 atctctgcag ggctacgact gcgtggagta ctatgccacc aggtataaca atttcccacg
     1201 cacactgagc atcatccacc ccaaggccta ctccaagctg gccaagcaca tccacgacaa
     1261 ttgggaggag atcaggttta tcaaggagaa cgagaacagc atgatcaagc ccgatatgca
     1321 cgccgacggc aggatcatca tcatgaatta cgaggatgcc gagaccaaga caatcaggga
     1381 gctgaacgac ggattcggca ggcgctttaa ggtgaacgcc gatatcagcg gctgtttcac
     1441 caatatctat tctcacagca tcccttgggc cgtgatcggc gtgaacaatg ccaagatcgc
     1501 cctgaacaca aaggtgaaga atcaggacaa gcactggtct gataagctgg actactttca
     1561 gcggcaggcc aagagaaacg agacccacgg agtgcctatc ggaccagcca catcctctat
     1621 cgtgtgcgag atcatcctga gcgccgtgga taagaggctg cgcgacgatg gcttcctgtt
     1681 tcggagatac atcgacgatt acacctgcta ttgtaagaca cacgacgatg ccaaggagtt
     1741 cctgcacctg ctgggcatgg agctgagcaa gtataagctg tccctgaacc tgcacaagac
     1801 caagatcaca aatctgcctg gcaccctgaa cgacaattgg gtgtctctgc tgaacgtgaa
     1861 tagcccaacc aagaagcggt tcacagatca ggacctgaac aagctgagct cctctgaagt
     1921 gatcaacttc ctggattacg ccgtgcagct gaacacacaa gtgggcggcg gctccatcct
     1981 gaagtacgcc atcagcctgg tcatcaacaa tctggatgag tataccatca cacaggtgta
     2041 cgactatctg ctgaatctgt cctggcacta ccccatgctg atcccttatc tgggcgtgct
     2101 gatcgagcac gtgtacctgg acgatggcga cgagtataag aacaagttca atgagatcct
     2161 gtctatgtgc gccgagaaca agtgcagcga tggcatggcc tggaccctgt acttctgtat
     2221 caagaacaat atcgacatcg acgatgacgt gatcgagaag atcatctgct ttggcgattg
     2281 tctgtccctg tgcctgctgg atagctccga catctatgag gagaagatca acaatttcgt
     2341 gtctgatatc atcaagctgg actacgagta tgatatcgac cggtactggc tgctgtttta
     2401 tcagagattc tttaaggaca aggccccaag cccctacaac gataagtgtt tcgacatcat
     2461 gaagggctat ggcgtggact tcatgcctga cgagaattac aagacaaagg ccgagtccta
     2521 ttgccacgtg gtgaacaacc cctttctgga agacggagac gagattgtga gtttcaacga
     2581 ctacatggct atcgcatgac ttttaggcct cattgaattc gcaataacta gcataacccc
     2641 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa acctcaggca tttgagaagc
     2701 acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg
     2761 ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc
     2821 attcatccgc ttattatcac ttattcaggc gtagcaccag gcgtttaagg gcaccaataa
     2881 ctgccttaaa aaaattacgc cccgccctgc cactcatcgc aatactgttg taattcattt
     2941 aacattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc
     3001 atcagcacct tgtcgccttg cgtataatat ttgcccatag tgaaaacggg ggcgaagaag
     3061 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     3121 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     3181 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     3241 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     3301 catatcacca gctcaccgtc tttcatcgcc atacggaact ctggatgagc attcatcagg
     3361 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     3421 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     3481 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     3541 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     3601 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
     3661 tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac accaggattt
     3721 atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt
     3781 gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc
     3841 tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag
     3901 caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg cactgatgag
     3961 ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga
     4021 atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
     4081 gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga
     4141 agatacttaa cagggaagtg agagggccgc ggcaaagccg tt
//
