LOCUS       sequence-320574-        4114 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the DRT type 2 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG010 (DRT type 2)
FEATURES             Location/Qualifiers
     source          1..4114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2018..2065
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2285..2944)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2859..2878
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2945..3047)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(3439..3456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(3573..4114,1..4))
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3653..3672)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaagaagat catcttatta atcagataaa atatttctag atttcagtgc aatttatctc
       61 ttcaaatgta gcacctgaag tcagccccat acgatataag ttgtaattct catgttagtc
      121 atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga
      181 gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgcaa cacgatataa
      241 aaccatctca ttgcttgctg ggttaactga gttgctgaat ttttttctag aatttcgcaa
      301 aatttaatag gtaaaccttg tttttttaaa tttacgatga tataaaaata atgccctaaa
      361 caaaggttta ggggtattgt acaggttgtc aagcctccca caggtcttgg tgaaaccaat
      421 cactgtgacg acggtaagca acacttggat gatattcata attgactcca cgctactgat
      481 tacattatac agcatatcta acatttgcgg cgaggttcac aatttgtatt taggtactga
      541 ttgtggatga gaaggttgga gaaagaccac ttggttaagc cggaggatgt gtcctagaat
      601 tgtcgctatt ctgtcatcct ccggttttgc taatttcatt cagggaatat aatgaataat
      661 gatgattacc catggttcag aaaacgtggt tatttgcatt tcgatgaacc tgtttcatta
      721 aaaaaagcgg ttaaatatgt ttcctctcca gaaaaaataa taaaacattc ttttctgcca
      781 tttttaagct ttgaagtaaa atcgtttaaa atcaaaaaag acaaatcaac aaaacaatta
      841 agtaaaactg aaaaattaag acctattgcc tattcctcac atttggatag tcatatttat
      901 gcattttacg cagaatatct tactggacat tatgaattat tgatccaaga aaacaattta
      961 cacgagaaca tccttgcctt cagatcttta aataaaagca atatagaatt tgccaagaga
     1021 gcatttgata caattactga aatgggtgag tgtagcgctg ttgcattaga tctttctggt
     1081 ttttttgaca atttagatca tcaaattttg aaacaccagt ggtgcaaagt tattgggact
     1141 gaagcgttgc cgcaagacca ttttgccata tacaaaagta taacaagata ttctaaagtt
     1201 gataaaaata gagcgtatga gattttaggt atatcaaaga ataaccccaa gtataataga
     1261 cgcaagatct gcacccctgt tgattttaga aataagatta gaaaaaatgg tcttattata
     1321 gttaataatt cccaaaaagg tataccccaa ggctcgccaa ttagtgctct actttcaaat
     1381 atatatatgc ttgactttga tattgaaatg agagattacg cgcaggaacg tggtggccat
     1441 tattatcgct attgtgatga tatgctattc attgtaccaa ctaagtataa taaaactcta
     1501 gcaggtgatg tagcccagcg gattaagcat cttaaggtag aactcaatac taagaaaact
     1561 gagattcgag attttatata caaagacagt accttagtgg caaatatgcc tttacagtat
     1621 cttgggttta tttttgatgg gagtaatata ttattacgtt catcttctct cgcaagatat
     1681 tcggaacgaa tgaaaagagg tgtccgctta gcaaaagcta caatggacag caagaatagg
     1741 attagagaaa ataaaggtga agctttaaaa gctttattta agaaaaaatt atatgccaga
     1801 tattcacata ttggaagaag gaattttttg acttatggtt atcgcgccgc gaagatcatg
     1861 aattcgaaag ctataaaaag acagttaaaa ccattgcaga aaagattgga aaatgaaata
     1921 ctaaaataaa tatttgctgg cccgaatcat acagggccac aatacagttg aaaacaagct
     1981 ataataaaca acatctaatt tttatatacc gcaataacta gcataacccc ttggggcctc
     2041 taaacgggtc ttgaggggtt ttttgctgaa acctcaggca tttgagaagc acacggtcac
     2101 actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg
     2161 accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc
     2221 ttattatcac ttattcaggc gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa
     2281 aaaattacgc cccgccctgc cactcatcgc aatactgttg taattcattt aacattctgc
     2341 cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct
     2401 tgtcgccttg cgtataatat ttgcccatag tgaaaacggg ggcgaagaag ttgtccatat
     2461 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     2521 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     2581 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     2641 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     2701 gctcaccgtc tttcatcgcc atacggaact ctggatgagc attcatcagg cgggcaagaa
     2761 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     2821 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     2881 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     2941 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     3001 atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc
     3061 gccaaaagtt ggcccagggc ttcccggtat caacagggac accaggattt atttattctg
     3121 cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca
     3181 acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc
     3241 agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga
     3301 catcagcgct agcggagtgt atactggctt actatgttgg cactgatgag ggtgtcagtg
     3361 aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata
     3421 caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg
     3481 agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa
     3541 cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag
     3601 catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac
     3661 caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
     3721 ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg
     3781 gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg
     3841 cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact
     3901 ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag
     3961 gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca
     4021 aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt
     4081 cagagcaaga gattacgcgc agaccaaaac gatc
//
