LOCUS       sequence-320603-        5869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses miniMAVS (the sequence starting from M142 of the MAVS CDS)
            with an N-terminal 3X flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Brubaker SW, Gauthier AE, Mills EW, Ingolia NT, Kagan JC
  TITLE     A Bicistronic MAVS Transcript Highlights a Class of Truncated 
            Variants in Antiviral Immunity.
  JOURNAL   Cell. 2014 Feb 13;156(4):800-11. doi: 10.1016/j.cell.2014.01.021.
  PUBMED    24529381
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.01"; journalName: "Cell"; date: "2014-02-13- 
            13"; volume: "156"; issue: "4"; pages: "800-11"
COMMENT     Sequence Label: p3XFlag-CMV7-miniMAVS(KaganE48)
FEATURES             Location/Qualifiers
     source          1..5869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        178..557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        558..761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     712..732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             791..856
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    2070..2692
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2269..2288)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2716..2736)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2721..3050
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2901..3036
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3088..3107)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3089..3107)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3121..3137)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3121..3137)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3134..3156)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3145..3161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3169..3175,3176..3193,3194..3199))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3214..3235
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3352..3369)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3523..4111)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3603..3622)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4282..5073,5074..5142))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4905..4924
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5143..5247)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5274..5729
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5361..5380)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5571..5592
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     join(5855..5869,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(5869,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagctgatct atacattgaa tcaatattgg caattagcca
       61 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
      121 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac
      181 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      421 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      481 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
      541 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
      601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      661 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
      781 attcaccatg gactacaaag accatgacgg tgattataaa gatcatgata tcgattacaa
      841 ggatgacgat gacaagcttc ctgtccagga gacccaggcg ccagagtccc caggagagaa
      901 ttcagagcaa gccctgcaga cgctcagccc cagagccatc ccaaggaatc cagatggtgg
      961 ccccctggag tcctcctctg acctggcagc cctcagccct ctgacctcca gcgggcatca
     1021 ggagaaggac acagaactgg gcagtaccca cacagcaggt gcgacctcca gcctcacacc
     1081 atcccgtggg cctgtgtctc catctgtctc cttccagccc ctggcccgtt ccacccccag
     1141 ggcaagccgc ttgcctggac ccacagggtc agttgtatct actggcacct ccttctcctc
     1201 ctcatcccct ggcttggcct ctgcaggggc tgcagagggt aaacagggtg cagagagtga
     1261 ccaggccgag cctatcatct gctccagtgg ggcagaggca cctgccaact ctctgccctc
     1321 caaagtgcct accaccttga tgcctgtgaa cacagtggcc ctgaaagtgc ctgccaaccc
     1381 agcatctgtc agcacagtgc cctccaagtt gccaactagc tcaaagcccc ctggtgcagt
     1441 gccttctaat gcgctcacca atccagcacc atccaaattg cccatcaact caacccgtgc
     1501 tggcatggtg ccatccaaag tgcctactag catggtgctc accaaggtgt ctgccagcac
     1561 agtccccact gacgggagca gcagaaatga ggagacccca gcagctccaa cacccgccgg
     1621 cgccactgga ggcagctcag cctggctaga cagcagcttt gagaataggg gccttgggtc
     1681 ggagctgagt aagcctggcg tgctggcatc ccaggtagac agcccgttct cgggctgctt
     1741 cgaggatctt gccatcagtg ccagcacctc cttgggcatg gggccctgcc atggcccaga
     1801 ggagaatgag tataagtccg agggcacctt tgggatccac gtggctgaga accccagcat
     1861 ccagctcctg gagggcaacc ctgggccacc tgcggacccg gatggcggcc ccaggccaca
     1921 agccgaccgg aagttccagg agagggaggt gccatgccac aggccctcac ctggggctct
     1981 gtggctccag gtggctgtga caggggtgct ggtagtcaca ctcctggtgg tgctgtaccg
     2041 gcggcgtctg cactagtcta gaggatcccg ggtggcatcc ctgtgacccc tccccagtgc
     2101 ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa
     2161 gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg
     2221 tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac
     2281 caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag
     2341 cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca
     2401 gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca
     2461 actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt
     2521 gaaccactgc tcccttccct gtccttctga ttttaaaata actataccag caggaggacg
     2581 tccagacaca gcataggcta cctggccatg cccaaccggt gggacatttg agttgcttgc
     2641 ttggcactgt cctctcatgc gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc
     2701 ggccagcttg gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     2761 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     2821 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     2881 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     2941 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     3001 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcctcg
     3061 aggaactgaa aaaccagaaa gttaattccc tatagtgagt cgtattaaat tcgtaatcat
     3121 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     3181 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     3241 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     3301 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     3361 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3421 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3481 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3541 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3601 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3661 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3721 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3781 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3841 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3901 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3961 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4021 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4081 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4141 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4201 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4261 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     4321 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     4381 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     4441 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     4501 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     4561 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     4621 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     4681 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     4741 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     4801 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     4861 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     4921 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4981 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5041 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5101 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5161 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     5221 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc
     5281 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5341 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5401 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5461 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5521 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5581 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5641 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5701 ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg caactgttgg
     5761 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     5821 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgtt
//
