LOCUS       sequence-320604-       20460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Polycistronic expression of ekivR, luc, esyn1 using P2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20460)
  AUTHORS   Schuetze T, Meyer V
  TITLE     Polycistronic gene expression in Aspergillus niger.
  JOURNAL   Microb Cell Fact. 2017 Sep 25;16(1):162. doi: 
            10.1186/s12934-017-0780-z.
  PUBMED    28946884
REFERENCE   2  (bases 1 to 20460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2017-09-25"; volume: "16(1):162. doi"; pages: " 
            10.1186/s12934-017-0780-z"
COMMENT     Sequence Label: pTS37.13
FEATURES             Location/Qualifiers
     source          1..20460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          245..360
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             join(378..995,996..1001,1002..1037,1038..1040,1041..1076,
                     1077..1079,1080..1115,1116..1124)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    join(1472..1490,1491..1513,1514..1532,1533..1555,1556..1574,
                     1575..1597,1598..1616,1617..1639,1640..1658,1659..1681,
                     1682..1700,1701..1723,1724..1742)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     intron          1812..1927
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             3327..3383
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5131..5187
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(17266..17285)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(17267..17285)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(17292..17309)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(17292..17308)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17301..17323)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(17449..17904)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(17586..17607)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     17798..17817
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        17931..18035
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(18036..18104,18105..18896)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(18254..18273)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      19067..19655
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     19556..19575
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     19809..19826
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    19943..19964
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(19979..19984,19985..20002,20003..20009)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    20017..20033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     20022..20044
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     20041..20057
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     20041..20057
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        20078..20096
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     20124..20140
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     20125..20141
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caagctggca gtcgacccat ccggtgctct gcactcgacc tgctgaggtc cctcagtccc
       61 tggtaggcag ctttgccccg tctgtccgcc cggtgtgtcg gcggggttga caaggtcgtt
      121 gcgtcagtcc aacatttgtt gccatatttt cctgctctcc ccaccagctg ctcttttctt
      181 ttctctttct tttcccatct tcagtatatt catcttccca tccaagaacc tttatttccc
      241 ctaagtaagt actttgctac atccatactc catccttccc atcccttatt cctttgaacc
      301 tttcagttcg agctttccca cttcatcgca gcttgactaa cagctacccc gcttgagcag
      361 acatcaccga attcaccatg tctagactgg acaagagcaa agtcataaac ggcgctctgg
      421 aattactcaa tggagtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg
      481 gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc
      541 tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat
      601 ggcaagactt tctgcggaac aacgccaagt cattccgctg tgctctcctc tcacatcgcg
      661 acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa
      721 atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt
      781 ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa
      841 aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg
      901 agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat
      961 gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc ggccgacgcc cttgacgatt
     1021 ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat atgctgcctg
     1081 ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtaactaagt aaggatccac
     1141 tagtacagca gaagaatctc tctccgctgt tgcttcagtg tctgccatgc attaacttca
     1201 tcctactgtc ctacccgcag tacccattca catttgcggc agataccagg ttgttttcta
     1261 ttccctcggt tcttcagttc ttcagatatt acattactcg agaaattggc ggcgatgcag
     1321 gagtttggtt ggttttaatt gtttcagtct ccttgacctg atttgtatga catgcaatgt
     1381 ttcgacggaa gactatctcg gtgaatatac gtcatgaatc atgcggccgc gcgtatcacg
     1441 aggccctttc gtcttcactc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     1501 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     1561 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     1621 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     1681 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     1741 gaaaagtgaa agtcgagctc cccatcttca gtatattcat cttcccatcc aagaaccttt
     1801 atttccccta agtaagtact ttgctacatc catactccat ccttcccatc ccttattcct
     1861 ttgaaccttt cagttcgagc tttcccactt catcgcagct tgactaacag ctaccccgct
     1921 tgagcagaca tcaccgttta aacaccatgg aaaagcctgt ggcctcacaa gtccacccca
     1981 gctggctaac agctcttcta cctgaatcca gaataccccc caagctgttt gcctggtcct
     2041 cagccaacat ccgcccagca agctcctcca agctcggagg ctctgccacc aacatccacc
     2101 atgcagagtg ttcacatcca accccaaact ccacagcaag ctctgagcag cgcatcttca
     2161 tcgttggtcc cggcaacata ggtaggctct acgcaagcca catggtccgc cgcccaaatc
     2221 ccctccccat cactctcgtc gtccccagaa aggaactcct atcgcaatgg gcaggctgtg
     2281 aaggcgtggg ccttatagac ccgggtcgcg gcaaggtcct caaaagcaag cgcttcaccg
     2341 tcgagtggtg gacggagact agaccacagt atggtcctgt gagagaggtg gcagacggaa
     2401 agaagctgcg caacgtgttc atatccacaa aggcatccgc tgggctggcc gaggcagatc
     2461 gtcttcgtcg gtatttgggc aagtgtagtt ctgtcgtctt tgcgcagaac ggagtatgca
     2521 agctgtggcc tcctcatggg ccgttatacg tcgccggtcg ataccctcct ggtgatgctc
     2581 ctaccttctc atcctgcgtc gtgaatcatg gcgttgcatc agcggggcct tttctgagca
     2641 ttcacgcagc tccggcagat gcatccatcg gacctgtcct ctggccttcc catccgatgc
     2701 cgcacaagaa acccctgaac gacttcttca caagatacat tgctactaca cccttcctaa
     2761 acacaaagca agtttcctct ggtgagctct ggcttattca gctggagaaa cttgttacca
     2821 atgccgccat caaccccctc acagctttgc tacgctgcaa aacgggacag ctgttcacaa
     2881 cccatgaccc cacagaccct cttacgcgcg tgctggacag gttgctatgg caggccagcg
     2941 ccgtgattca gggtcttatc aaccacgacg cgagtgtcga catcgtcgct tcctacgccg
     3001 agcacctcca ccagaccgaa ccaagcagcg actcaagcct tgtcgcagta cggaccaagt
     3061 taacagagcg gttctcccag cctaccttga gagcgaagct attcgccttt ggtcgcaaga
     3121 tatacgagca tcgcagctcg atgcttcagg atgttgaggc cggcaggaaa acggagataa
     3181 gagatttcaa cgggtgggtt gtggatatgg cgagttttct ggggacgggg ctgaatgtga
     3241 gcgtgcatca gagtttgatt gtgctgattg aacgcggtga ggttttgaac aaggatgagt
     3301 tggctaggag gacattaggc tccggcgcca ccaacttctc cctcctgaag caggccggtg
     3361 acgtcgagga gaaccccggc cccatggagg acgccaagaa catcaagaag ggccccgccc
     3421 ccttctaccc cctcgaggac ggcaccgccg gcgagcagct ccacaaggcc atgaagcgct
     3481 acgccctcgt ccccggcacc atcgccttcg taggtttcct ccagctctcg cctccagcac
     3541 ccgaggcaca cctcgggcat cttcacaaca acagacactg acatctcatt ctcacagacc
     3601 gacgcccaca tcgaggtcaa catcacctac gccgagtact tcgagatgtc cgtccgcctc
     3661 gccgaggcca tgaagcgcta cggcctcaac accaaccacc gcatcgtcgt ctgctccgag
     3721 aactccctcc agttcttcat gcccgtcctc ggcgccctct tcatcggcgt cgccgtcgcc
     3781 cccgccaacg acatctacaa cgagcgcgag ctcctcaact ccatgaacat ctcccagccc
     3841 accgtcgtct tcgtctccaa gaagggcctc cagaagatcc tcaacgtcca gaagaagctc
     3901 cccatcatcc agaagatcat catcatggac tccaagaccg actaccaggg cttccagtcc
     3961 atgtacacct tcgtcacctc ccacctcccc cccggcttca gcgagtacga cttcgtcccc
     4021 gagtccttcg accgcgacaa gaccatcgcc ctcatcatga actcctccgg ctccaccggc
     4081 ctccccaagg gcgtcgccct cccccaccgc accgcctgcg tccgcttctc ccacgcccgc
     4141 gaccccatct tcggcaacca gatcatcccc gacaccgcca tcctctccgt cgtccccttc
     4201 caccacggct tcggcatgtt caccaccctc ggctacctca tctgcggctt ccgcgtcgtc
     4261 ctcatgtacc gcttcgagga ggagctcttc ctccgctccc tccaggacta caagatccag
     4321 tccgccctcc tcgtccccac cctcttctcc ttcttcgcca agtccaccct catcgacaag
     4381 tacgacctct ccaacctcca cgagatcgcc tccggcggcg cccccctctc caaggaggtc
     4441 ggcgaggccg tcgccaagcg cttccacctc cccggcatcc gccagggcta cggcctcacc
     4501 gagaccacct ccgccatcct catcaccccc gagggcgacg acaagcccgg cgccgtcggc
     4561 aaggtcgtcc ccttcttcga ggccaaggtc gtcgacctcg acaccggcaa gaccctcggc
     4621 gtcaaccagc gcggcgagct ctgcgtccgc ggccccatga tcatgtccgg ctacgtcaac
     4681 aaccccgagg ccaccaacgc cctcatcgac aaggacggct ggctccactc cggcgacatc
     4741 gcctactggg acgaggacga gcacttcttc atcgtcgacc gcctcaagtc cctcatcaag
     4801 tacaagggct accaggtcgc ccccgccgag ctcgagtcca tcctcctcca gcaccccaac
     4861 atcttcgacg ccggcgtcgc cggcctcccc gacgacgacg ccggcgagct ccccgccgcc
     4921 gtcgtcgtcc tcgagcacgg caagaccatg accgagaagg agatcgtcga ctacgtcgcc
     4981 tcccaggtca ccaccgccaa gaagctccgc ggcggcgtcg tcttcgtcga cgaggtcccc
     5041 aagggcctca ccggcaagct cgacgcccgc aagatccgcg agatcctcat caaggccaag
     5101 aagggcggca agtccaagct cggctccggc gccaccaact tctccctcct gaagcaggcc
     5161 ggtgacgtcg aggagaaccc cggccccatg tcactccaca ccccaagtga cggtcaacaa
     5221 gacccagcgt tagcctccaa aaccctctgt gagcaaatct cgcgagccct cggcctcggc
     5281 caggacaaga ttgagaatat cttcccaggg acacccttcc agcgggacgt catagactgc
     5341 gccgcagacg acaagcagcg cgccgtgggc cacgctgtct ttgaaattcc caaagatata
     5401 gacgcggccc gcctggcggc tgcgtggaag gagaccgtgc ttcacacccc tgctctgcgg
     5461 acctgcacgt ttacgtccaa atctggggat gtgcttcagg tggtcctgag agatagcttt
     5521 gtcttttcgt ggatgtctgg gccgtctgtg gatcttaagg aggcggttgt gcaagacgag
     5581 gctgctgctg ctttggctgg cccgcgctgt aaccgcttcg tccttcttga ggaccctgat
     5641 acaaaggaac gtcagctcat ctggacgttc agccatgctc tcgttgatag taccttccag
     5701 gaacgtatcc ttcgacgagt cctcaaagct tacaaggatg ccaatgacga acatcctcgc
     5761 cagttcgaga caccagattc ttcccaggcc acgcccgagg aggatttaca gcccaatcca
     5821 tccaagatgc tcaagatccc tcaagccgca gatatggacc gtgctgttga gttttggaag
     5881 gatcatctaa gtggcctgaa tgcttctgcc ttcccgcatt tgtcctctca tctgtctatg
     5941 ccacatccag atgccaaggc agagcataga atctcctact cgtcttcagc acagcaaaaa
     6001 atgtccagtg ctactatttg ccgaactgcc ctggcaattc tgttatctcg ttatacgcac
     6061 tctcctgaag cactctttgg cattgtaact gagcaaactc ctctactgga ggagcaactc
     6121 atgcttgatg gcccaacacg tacagtcgta ccaatccgcg tatcctgtgc ttctgaacag
     6181 tcagtgtcag atatcatgag cacgattgac tcctacgatc agaccatgcg gcagtttgcc
     6241 cacgctggtc ttcgcaacat cgccagtgct ggagacgatg aatctgccgc ctgcgggttc
     6301 cagaccgttc tcttggtctc agatggagac gcccagccag catctacttg ggaaattctc
     6361 aagaaaactg aagagcctga gggtttcatc ccctgtacga accgtgctct gcttctaagt
     6421 tgccagatga ccagctctgg agcacaccta accgccagat acgaccagag tatcatcgac
     6481 gctgagcaaa tggcccgact cctgcgacaa ctaggccatc tgatccaaaa tcttcaaacc
     6541 tccacagatc tacccgtgga aaaggtggac atgatgacgc aagaagactg gttagagatt
     6601 gagaggtgga actcagactc catcgacgca caagacacgc ttatccacag cgagatgttg
     6661 aagtggactt ctcagtcgcc taacaaagct gccgtcgccg cgtgggatgg agagtggacg
     6721 tatgctgagc tggacaatgt atcttcgcgc ctggctcagc atatcaactc tattgacctg
     6781 ggcaaggagc acgcgatagt acccatctac tttgagaaat cgaaatgggt cgttgcttcg
     6841 atgctggctg tgctcaaggc tggccatgcg tttactctca ttgaccccag tgatccacca
     6901 gctcgaacag cacaggtcgt gcagcaaacc tctgcgacag ttgctcttac ttccaagctc
     6961 catcgcgaga ctgttcaatc aactgtcggg cgctgcattg ttgtcgacga agagtttgtc
     7021 aaatccctcc cccagtccag cgaactctca gcgtctgtaa aggcacatga cctggcatat
     7081 gtcatcttca cctcgggcag cacaggcatt ccaaagggaa tcatgattga gcaccgctca
     7141 ttttcatcct gcgccatcaa attcggtccc gcgcttggaa taacctccga cacacgcgct
     7201 cttcagtttg gatctcatgc ctttggcgcg tgtatcctcg agatcatgac gacgctcatc
     7261 cacggaggct gtgtctgtat cccctctgac gatgaccgca tgaacaacgt ccttgagttc
     7321 atcaaccgga ctaacgtcaa ctgggtcatg gcaacgcctt cgtacatggg gacattccag
     7381 ccagaggttg ttcctggttt gaagaccttg gtattggtag gtgagcagat gtctgcttct
     7441 gtgaatgagg tctgggctcc tcgtgttcaa ctcctgaacg gttacggaca gagcgagagt
     7501 tcttctatct gctgcgtagc caagatcagc cctggttcat ctgagccaaa caatattggc
     7561 cacgcagtag gcgcacattc ttggatcgtt gaccctgagg atcccaatcg cttggctccc
     7621 atcggtgccg ttggcgagct ggtcatcgag agtgcaggta tcgcacgcga ttacatcgtt
     7681 gcgccaactc aggataagtc tcctttcatt aagactgctc ctacgtggta tccggccaag
     7741 cagctcccgg acggcttcaa gatctacaga acaggcgatc ttgcatgcta cgcatctgat
     7801 ggcagtatag tgtgccttgg ccgtatggac tcccaggtca agatcagagg acagcgtgtt
     7861 gagctgggtg cagtggagac tcatcttcga cagcagatgc ctgatgatat gaccatcgtt
     7921 gttgaagctg tcaagttctc tgattcctcc agtactacag ttttgacagc cttcttgatt
     7981 ggtgcagggg agaagaacag tcatatcttg gaccagcgtg ctacaaggga gatcaatgcc
     8041 aagatggagc aagttcttcc tcgccattcc atccccgcgt tctacatctc tatgaacaac
     8101 ctcccccaaa cagccactgg taaggtcgat cgacgaaaac ttcgtatcat gggtagcaag
     8161 atactgagcc aaaagacgca ttccacccca tctcagcaga gtcaggccgc catctcatct
     8221 ggaaccgaca cagagaccaa gctggagagc atctggatca caagcttgga cctcgagccc
     8281 ggctctgcaa acatgtcagc tacattcttc gagatgggcg gaaactccat catcgcaatc
     8341 aagatggtca acatggcgcg ctccaatggc atagagctta aggtctcgga catttaccag
     8401 aaccccacgc tcgctggtct caaggctatt gtcattggta cttcgctgcc atacagcctt
     8461 attcccaagg ttacccgcca aggccctgtt agcgagcagt cttatgcgca aaacagaatg
     8521 tggttcctgg atcagttgtc tgagggtgct tcatggtatc tgattccttt cgctgtgcgc
     8581 atgcgaggtc cggtggatgt tgatgcgttg acgcgtgctt tgctcgctct tgaacagcgt
     8641 catgagacac tacgaactac atttgagaac caagatggtg ttggagtcca gatcatccat
     8701 gatagactct ccaaagagct acaagtcatc gatgccttgg atggtgacga gggtggtctc
     8761 aagacactct acaaagtaga gaccaccaca ttcgacatca catccgaagc aggctggagc
     8821 tcaaccctca tccgcctcgg caaagacgat cacattctgt ctatcgtcat gcaccacatc
     8881 atctccgacg gctggtccat cgacgttctc cgccgcgaac tcatccaact ctacgccgcc
     8941 gctctgcagg gcaaggatcc ttcctccgca ctaacccccc tacccatcca gtacagcgac
     9001 ttcgccgtgt ggcagaagca ggaggcccaa gcagctgagc acgagaggca gctccagtac
     9061 tggaagaagc aactcgcaga tagttcacct gccaagatcc ctaccgactt cccccgtcca
     9121 gatctcctgt ccggtgacgc aggcgttgtg cccgttgcca tcgacggcga gctgtatcag
     9181 aaactaaggg gcttctgcaa caagcacaac agcactgcct tctccatcct gctcgctgct
     9241 ttccgcgcgg cgcattaccg tctcacagcc gttgacgacg ccgtgatcgg catccccatt
     9301 gcaaaccgta accgctggga gctggagaac atgattggtt tctttgtcaa cacgcagtgt
     9361 atgcgcatcg ccgttgacga gacggataca tttgagagtc tggtgcgcca ggtcagatct
     9421 accactacag ctgcgtttgc gcacgaggat gtccccttcg agcgtgtcgt ttcagcgctt
     9481 cagcctggcc atagagatct ctcgcgaaca ccgctggcac agataatgtt tgctgttcac
     9541 tcgcagaagg accttggacg tttcgagctg gagggtatcc agtctgagcc tatcgccagc
     9601 aaggcctaca ccagattcga tgtcgagttc catctgttcc aacaggcaga cggactgaag
     9661 ggcagttgca actttgccac agatctcttc aagcccgaaa ctatccagaa tgttgtcagc
     9721 gtgtttttcc agattctacg ccatggcctt gaccagcctg agacgtgtat ctcggttctt
     9781 ccactgactg atggagtcga ggagcttcgc aggttggatc tgctggaaat caagaggact
     9841 aactaccctc gcgattcgag cgtggtagat gtcttccgcg aacaagctgc cgccaaccca
     9901 gaggttatcg ctgttaccga ctcatcttct cgtctgactt atgcagagct ggacaataag
     9961 tctgagctgc tctcacgatg gcttcgacga cgtaacttga cgccagagac gctggtcagt
    10021 gttcttgctc cccggtcttg cgagactatc gttgcctatg ttggtatcct caaggcgaac
    10081 ctggcgtatc ttcctcttga cgtgaggtcc ccggtgactc gtatgaagga catcttgtcg
    10141 agcgtgtctg gaaacaccat agttcttatg ggctctgggg tagaggatcc tggctttgac
    10201 ttaccgcaac tagagctcgt acgcatcacc gacactttcg atgagaccat cgaggacgtg
    10261 caagactctc cccaaccgtc tgccacaagc ctcgcctacg tcgtcttcac atccggttca
    10321 actggtaaac ccaagggcgt catgatcgag caccgggcca ttgtgcgtct cgtcaagagt
    10381 gacaactttc ctggcttccc ctcccccgct cgcatgtcaa atgtcttcaa ccctgccttc
    10441 gacggagcca tctgggagat caactggatg ctcctgaacg gcggaacagt cgtctgcatc
    10501 gactacctga ccaccctgga cggcaaagag ctcgctgctg tgttcgccaa agagcgcgtc
    10561 aacgccgcct tcttcgcacc tgcgatgctc aagctttacc tcgttgatgc gcgcgaggct
    10621 ttgaagaatc ttgacttcct tattgttgga ggtgagaggt tcgataccaa ggaagccgtg
    10681 gaggccatgc cgcttgtgag aggcaagatt gccaatatct atggtccgac tgaggctgga
    10741 ataatcagca cgtgctataa catccccaag gatgaggctt acaccaatgg tgttcccatt
    10801 ggtggaagta tctacaactc tggtgcctac gtcatggatc ctaaccagca acttgtcggc
    10861 cttggcgtca tgggagagct tgttgttacc ggagacggtg ttggtcgagg ctacactaac
    10921 cccgagctca acaagaaccg cttcatcgac atcaccatcg agggcaagac tttcaaggct
    10981 taccgtactg gtgaccggat gcgtgcacga gtaggcgacg gtctccttga gttcttcggc
    11041 cgcatggaca accagttcaa gatccgcggc aaccgtatcg aagcagggga agttgagtct
    11101 gccatgctca gcctcaagaa tgtccttaac gccgccattg tcgtccgcgg gggcggagaa
    11161 gatgaagggc cactcgagat ggtcggattc atcgtcgcgg acgacaagaa tgataccacg
    11221 gaggaggaag agacaggcaa ccaagttgag ggctggcagg accatttcga gagtggtatg
    11281 tactcggata tcagcaccgc cgtggaccaa tctgccattg gaaacgactt taagggctgg
    11341 acttctatgt acgatggtaa ggatatcgac aagggcgaga tgcaggagtg gttggacgac
    11401 gctattcaca ccctgcataa cggccagatc cctcgcgatg tcctcgagat cggtaccggt
    11461 agtggtatga tcctgttcaa cctcaacccg ggcctcaaca gctacgttgg tcttgatcca
    11521 tccaagtcag cagtcgagtt cgtcaacaga gccgtcgagt cctctcccaa gttcgcagga
    11581 aaagccaagg tccacgtcgg catggccaca gacgtcaaca aactcggcga agtacacccc
    11641 gatctcgtgg tcttcaactc cgttgttcaa tacttcccca cacccgagta tctcgccgag
    11701 gtcatcgatg gcctcattgc catccccagc gtcaagcgca tcttccttgg cgatatcaga
    11761 tcatatgcta ccaacggaca cttcctcgcc gcacgcgcca tccacacgct cggcaccaat
    11821 aacaacgcca ccaaggatag ggtgcgccag aagatccagg agctggagga tcgagaggaa
    11881 gagtttctcg ttgagcctgc cttcttcacc actctgaagg agcgacgtcc agatgttgtc
    11941 aagcatgttg agatcatccc caagaacatg aaggccacca acgaactcag cgcctatcgc
    12001 tacacggctg ttgtgcatct gcgggatgaa acggacgaac ctgtgtatca tattgagaag
    12061 gatagctggg ttgactttga ggcgaagcag atggacaaga cggctcttct tgaccacctg
    12121 cgcctctcca aggatgctat gagtgtggcg gttagcaaca tcacctacgc ccacactgcc
    12181 tttgaacgtc gtatcgttga gtctctcgat gaggatagca aggatgacac caagggtaca
    12241 ctcgatggtg cagcctggct ctcagcagtt cgctccgaag ccgaaaaccg cgcctcactc
    12301 accgtccccg acatcctgga gatcgccaaa gaggctggtt tccgagttga agtcagcgct
    12361 gctcgccagt ggtcccaaag tggtgcttta gacgcagtct tccaccactt tccaccctcc
    12421 agcactgacc gcactctaat ccagttcccc acggacaacg agcttcgatc atcactcacc
    12481 ctcgccaacc gccctctcca gaagctgcag cgccgtcgtg ccgctctgca agtccgcgag
    12541 aagctccaga cgctggtccc gtcttacatg gttcctccga atatcgtggt gctggacacg
    12601 atgcctctca atactaacgg caagatcgac agaaaggagc ttacgcgtag agcacgaaca
    12661 ctgccgaagc agcagactgc ggcgcctgtg ccggacttcc ctatctctga tatcgagatc
    12721 acgctgtgcg aggaggcaac tgaggtcttt ggaatgaagg ttgaaatcag cgatcacttc
    12781 ttccagctcg gcggtcactc tctcctcgct acgaaactca tttctcgcat ccagcaccgt
    12841 ctccatgtgc gggttactgt gaaggacgta ttcgacagcc ctgtctttgc cgatctggca
    12901 gtcatcatcc gtcaaggact tgctatgcag aaccctgttg ctgaaggaca ggacaagcaa
    12961 ggctggtcct cgagagttgc ccctcgtaca gaagtcgaga agatgctgtg cgaggagttc
    13021 gcagctggtc ttggtgtccc ggttggtatc actgacaact tcttcgatct cggtggtcac
    13081 tcgctcatgg ctactaagct agctgtgcga attggccgtc gccttgatac cgccatcaca
    13141 gtcaaggaca tcttcgacta ccctgtgctt ttccaattgg cgaagaagtt ggagtcttcg
    13201 cattccaaga gctacgagga gtctggcgac gatatccaga tggccgatta cactgcattc
    13261 cagctcctcg atctggaaga cccccaagac tttgttcagt cccagattcg gcctcaactg
    13321 gactcctgct acggcaccat ccaggatgtc tacccgtcta cgcagatgca aaaggccttc
    13381 ctcttcgatc ccacgaccgg cgagccccga ggtcttgtgc ctttctatat cgacttcccc
    13441 agcaatgcag atgccgagac cctcaccaag gctatcggag ctctagttga caagctcgat
    13501 atgttcagaa ctgtcttcct ggaggccgca ggcgatctgt accaagttgt cgttgagcac
    13561 ctcaacttgc ctattgagac catcgagact gagaagaacg tcaacactgc aaccggtgac
    13621 tacctggatg ttcatggaaa ggaccctgtc cgtctaggcc acccgtgcat ccaattcgcc
    13681 atcctgaaga ctgcctcctc tgtacgtgtt ctcctgcgaa tgtcccacgc tctgtatgat
    13741 ggtttgagtt ttgagtacat cgtccgtggt ctccacgttc tctacagcgg tagaaacctc
    13801 cccccaccaa cacagtttgc gcgatacatg cagtatgctg cacacagtcg tgaagaaggt
    13861 tatcccttct ggcgcgaggt tctgcagaac gcgcccatga cagttctaca cgacaccaat
    13921 aacggtatgt ctgagcaaga gatgccagcc tccaaggcgg tacacctgtc agaggtcgtc
    13981 aacgttccag cgcaagctat tcgaaacagt accaacaccc aagcgaccgt cttcaacacc
    14041 gcctgtgccc ttgtcctagc caaggaatcc ggctcacagg atgtcgtctt cggccgtatt
    14101 gtctctggtc gacaaggtct accagtcgtc tggcaggaca tcatcggccc ctgcacaaac
    14161 gccgtgcccg tccacgcacg cgtcgacgat ggaaaccccc aacgcatcat ccgcgaccta
    14221 cgcgaccaat acctccgcac tctgcccttc gaatcgcttg gcttcgagga aatcaagcgt
    14281 aactgcacgg actggcccga agaattgacc aacttctctg tctgcgtgac gtaccacaac
    14341 ttcgagtacc accccgagag cgaagttgac aaccaaaagg ttgagatggg agttttggca
    14401 aagtatgttg agttgagtga aaacgagccg ctgtacgatc ttgctattgc gggagaggtt
    14461 gaggcggatg gagttaacct gaaggtcact gttgttgcaa aggcaaggct ttacaatgag
    14521 gcgaggatta gacatgtgct tgaggaagtt tgcaaaactt tcaatggttt gaacgaggct
    14581 ttgtaagttt aaacagtaga tgccgaccgg gatccactta acgttactga aatcatcaaa
    14641 cagcttgacg aatctggata taagatcgtt ggtgtcgatg tcagctccgg agttgagaca
    14701 aatggtgttc aggatctcga taagatacgt tcatttgtcc aagcagcaaa gagtgccttc
    14761 tagtgattta atagctccat gtcaacaaga ataaaacgcg tttcgggttt acctcttcca
    14821 gatacagctc atctgcaatg cattaatgca ttggacctcg caaccctagt acgcccttca
    14881 ggctccggcg aagcagaaga atagcttagc agagtctatt ttcattttcg ggagacgaga
    14941 tcaagcagat caacggtcgt caagagacct acgagactga ggaatccgct cttggctcca
    15001 cgcgggcgcg cctcggtcgc tcactgttcc tttacggata tgtgtactcc ggcaagccgc
    15061 ggggtcgcgg tcgtttgtac ggcagcttct acttgcttgc acagggagct ctctggggct
    15121 tgacgtcttt tggagttgcg agggagttga tttcctactt ctaagtttgg actgaatccg
    15181 tggtgtgatt gaggtgattg gcgatgtttg gctataccag ctatatgtaa taatctctac
    15241 tgtatactac tattcaacgc attttactat gcgtgctgct agggtcggca atgacaatgg
    15301 caatctgact gacgtggtct atttctccat gtgcagcagg gaatacgagc tccaatggac
    15361 ctcgggagtg gcacagtcaa tggcaaggaa actccgcctt tgcaggtgtg gctgaacccc
    15421 acgggtcgga ggcggagcaa tccacccccg atgtggctgg tgcgtggagg ggctcgcgat
    15481 gattttactg agcttgcttt tcttgtcgac attgaacatt gtccttggtc ttccttcaga
    15541 tttaagggtc agtcactgct acatttctca gtagtatccg cgcacgtctc tggatttacg
    15601 aatcagggtc caccagtcga aacttcgaac tactctcatt atacaatcct ctttccattc
    15661 ccgcattaac ccctccatca acaccatgtc ctccaagtcg caattgacct acactgcccg
    15721 tgccagcaag catcccaatg ctctggcgaa gaggctgttc gagattgccg aggccaagaa
    15781 gaccaatgtg actgtctcgg ctgacgttac caccactaag gagctactag atcttgctga
    15841 ccgtaggccg acccgctact ctgcctgatt atgctgcatg caaacttatt aacggtgata
    15901 ccggactgca ggtctcggtc cctacattgc cgtgatcaaa acccacatcg atatcctctc
    15961 tgatttcagc aacgagacca ttgagggact taaggctctc gcgcagaagc acaactttct
    16021 catcttcgag gaccgcaagt tcattgacat cggcaacacg gtccagaagc aataccacgg
    16081 cggtaccctc cgtatctcgg aatgggccca catcatcaac tgcagcattc tccctggtga
    16141 gggtatcgtc gaggctctcg ctcagacggc gtctgcaccg gacttcgcat acggccccga
    16201 acgcggtctg ttgatcttgg cagagatgac ctctaagggc tccttggcta ccggccagta
    16261 cactacttcc tcggtcgatt atgcccggaa atacaagaac ttcgttatgg gattcgtgtc
    16321 gacgcgcgcg ttgggtgagg tgcagtcgga agtcagctct ccttcggatg aggaggactt
    16381 tgtggtcttc acgactggtg tgaacatttc ttccaaggga gataagcttg gtcagcagta
    16441 ccagacgccc ggatcggcta tcggccgggg tgctgacttc attatcgcgg gtcgcggtat
    16501 ctacgccgcg ccggatcgat ccggtgcagg ctgcgcaaca gtatcagaag gaggggtggg
    16561 aagcctacct ggcccgtgtc ggcggaaact aatactataa aaggaggatc gaagttctga
    16621 tggttatgaa tgatatagaa atgcaacttg ccgcaacgga tacggaagcg gaaacggacc
    16681 aatgtcgagc acgggtagtc agactgcggc atcggatgtc caaacggtat tgatcctgca
    16741 ggctactatg gtgtggcaca aggatcaatg cggtacgacg atttgatgca gataagcagg
    16801 ctgcgaagta gtaactcttg cgtagagaaa atggcgacgg gtggctgata agggcggtga
    16861 taagcttaat tgtcatcgca gataagcact gctgtcttgc atccaagtca gcgtcagcag
    16921 aaatacggga cttccgaaag tatatggcaa aattaaagaa cttgactctc cagcaatgtt
    16981 ttgccctgac cgtcgctaaa acgttactac ccctataccc gtctgtttgt cccagcccga
    17041 ggcattaggt ctgactgaca gcacggcgcc atgcgggctt gggacgccat gtccgtcgcg
    17101 tgataagggt tgatccatgc agctactatc cttccatcgt tccattccca tccttgtcct
    17161 atctccatcc ttgaaacttt actagtttag ttggatgctc gagcttgctc tcggctactc
    17221 cgtcggcgcg ccttgcggcc gccaccgcgg tggagctcca attcgcccta tagtgagtcg
    17281 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
    17341 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
    17401 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa attgtaagcg
    17461 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
    17521 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
    17581 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
    17641 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
    17701 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
    17761 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
    17821 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
    17881 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
    17941 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    18001 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    18061 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    18121 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    18181 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    18241 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
    18301 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    18361 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    18421 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    18481 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    18541 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    18601 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    18661 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    18721 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    18781 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    18841 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    18901 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    18961 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    19021 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    19081 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    19141 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    19201 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    19261 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    19321 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    19381 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    19441 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    19501 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    19561 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    19621 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    19681 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    19741 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    19801 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    19861 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    19921 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    19981 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    20041 caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac
    20101 aaaagctggt accgggcccc ccctcgaggt cgacggtatc gataagcttc ggagaatatg
    20161 gagcttcatc gaatcaccgg cagtaagcga aggagaatgt gaagccaggg gtgtatagcc
    20221 gtcggcgaaa tagcatgcca ttaacctagg tacagaagtc caattgcttc cgatctggta
    20281 aaagattcac gagatagtac cttctccgaa gtaggtagag cgagtacccg gcgcgtaagc
    20341 tccctaattg gcccatccgg catctgtagg gcgtccaaat atcgtgcctc tcctgctttg
    20401 cccggtgtat gaaaccggaa aggccgctca ggagctggcc agcggcgcag accgggaaca
//
